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Tissue References Search Tool

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Cartilage
Rabbit
Rabbit, New Zealand, 1.8-2.3kg
Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.
Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion ,
Ann Rheum Dis
 56 , 108 , 1997
Cells
Chondrocytes
Enzymes
Medium
Gey's solution
Cartilage
Rabbit
Rabbit, white, male, 8 weeks
Benya, P.D. , Padilla, S.R. and Nimni, M.E.
The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II ,
Biochemistry
 16 , 865 , 1977
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Gey's BSS
Cartilage
Rabbit
Rabbit, New Zealand white or Dutch, 1 week
(also human, newborn)
Schindler, F.H. , Ose, M.A. and Solursh, M.
The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture ,
In Vitro
 12 , 44 , 1976
Cells
Chondrocytes
Enzymes
Trypsin : 0.1%
Medium
Saline G, CMF
Cartilage
Rabbit
Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg
Green, J.R. and William, T.
Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting ,
Clin Orthop Relat Res
 , 237 , 1976
Cells
Articular chondrocytes
Hyaline
Enzymes
Trypsin : 0.2%
Medium
Gey's BSS
Cartilage
Rabbit
Rabbit, New Zealand white, male, 250-350 g
Bentley, G. and Greer , R.
Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits ,
Nature
 230 , 385 , 1971
Cells
Epiphyseal
Articular cartilage
Enzymes
Trypsin : 0.25%
Medium
Eagle's basal medium
Endothelial
Porcine
Porcine
Shasby, S.
Endothelial Cells Grown On Permeable Membrane Supports ,
J Tiss Cul Meth
 14 , 247 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
Medium 199
Endothelial
Porcine
Porcine
Vischer, P. and Buddecke, E.
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells ,
Exp Cell Res
 158 , 15 , 1985
Cells
Endothelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Porcine
Porcine, 60-100 days
Coulson, W.F.
The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin ,
Biochim Biophys Acta
 237 , 378 , 1971
Cells
Aorta
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer
(see reference)
Epithelial
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Nicosia, S. , Johnson, J. and Streibel, E.
Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) ,
Int J Gynecol Pathol
 3 , 348 , 1984
Cells
Mesothelial and surface epithelial
Ovaries
Enzymes
Trypsin : 0.125%-0.5%
Medium
Medium 199
Eye
Rabbit
Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.
Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
 45 , 807-12 , 2004
Cells
Corneal keratocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.5%
Medium
PBS
Eye
Rabbit
Rabbit, New Zealand, adult, male, albino,
2 kg
Johnson-Muller, B. and Gross, J.
Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions ,
Proc Natl Acad Sci U S A
 75 (9) , 4417 , 1978
Cells
Epithelial
Enzymes
Trypsin : 0.25%
Medium
HBSS/DMEM
Heart
Porcine
Porcine
Christ, M. , Gunther, A. , Heck, M. , Schmidt, B. , Falkenstein, E. and Wehling, M.
Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells ,
Circulation
 99 , 1485 , 1999
Cells
Coronary vascular smooth muscle (PCVSMCs)
Enzymes
Trypsin : 0.037%
Medium
HEPES
Heart
Porcine
Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise
Bowles, D. , Hu, Q. , Laughlin, M. and Sturek, M.
Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle ,
Am J Physiol
 275 (44) , H2159 , 1998
Cells
Coronary smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Krebs bicarbonate solution
Heart
Rabbit
Rabbit, New Zealand, 3-3.5 kg
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Kidney
Rabbit
Rabbit, New Zealand, white
Grenier, F. and Smith, W.
Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae ,
Prostaglandins
 16 , 759 , 1978
Cells
Collecting tubule
Enzymes
Trypsin : 0.05%
Medium
Kreb's Ringer buffer
Lung
Rabbit
Rabbit, New Zealand, white
Scott, J.
The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung ,
Exp Lung Res
 12 , 181 , 1987
Cells
Alveolar type II
Enzymes
Trypsin : 0.05%
Medium
HBSS
Lung
Rabbit
Rabbit, New Zealand white, adult,male
Finkelstein, J. , Maniscalco, W. and Shapiro, D.
Properties of Freshly Isolated Type II Alveolar Epithelial Cells ,
Biochim Biophys Acta
 762 , 398 , 1983
Cells
Alveolar type II
Enzymes
Trypsin : 0.0025%
Medium
Joklik's MEM
Miscellaneous
Porcine
Porcine, 6 month
Kean, T. and Dennis, J.
Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions. ,
PLoS ONE
 10 , e0129961 , 2015
Cells
Synoviocytes
Enzymes
Hyaluronidase : 660 u/ml
Trypsin : 0.25%
Collagenase Type 2 : 583 u/ml
Medium
DMEM
Muscle
Porcine
Porcine, postnatal
Miersch, C. , Stange, K. and Rontgen, M.
Effects of Trypsinization and of a Combined Trypsin, Collagenase, and DNase Digestion on Liberation and in Vitro Function of Satellite Cells Isolated from Juvenile Porcine Muscles. ,
In Vitro Cell Dev Biol Anim
 54 , 406-412 , 2018
Cells
Muscle satellite
Enzymes
Medium
Eagle MEM
Muscle
Porcine
Porcine
Breton, M. , Berrou, E. , Deudon, E. and Picard, J.
Changes in Proteoglycans of Cultured Pig Aortic Smooth Muscle Cells During Subculture ,
In Vitro Cell Dev Biol
 26 , 157 , 1990
Cells
Smooth muscle
Aorta
Enzymes
Trypsin : 0.05%
Medium
EDTA 0.02%
Muscle
Rabbit
Rabbit, New Zealand, male, 2.5-3.5 Kg
Santos, M. , Nguyen, B.. and Thompson, J.
Factors Affecting in Vitro Growth of Harvested Enterocytes ,
Cell Transplant
 1 , 299 , 1992
Cells
Enterocytes
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640 w/ 1% fetal bovine serum
PBS
Muscle
Rabbit
Rabbit
(also rat, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rabbit
Rabbit, New Zealand white, 1500 g
Knodle, S. , Anderson, S. and Papaioannou, S.
Large Scale Preparation of Rabbit Aortic Smooth Muscle Cells For Use in Calcium Uptake Studies ,
In Vitro Cell Dev Biol
 22 , 23 , 1986
Cells
Smooth muscle, aortic
Enzymes
Trypsin : 0.038%
Medium
MEM
Muscle
Rabbit
Rabbit, adult, 1-2 Kg
Benham, C.D. and Bolton, T.B.
Spontaneous Transient Outward Currents in Single Visceral and Vascular Smooth Muscle Cells of the Rabbit ,
J Physiol
 381 , 385 , 1986
Cells
Smooth muscle, ear artery
Enzymes
Trypsin : 0.1%
Medium
CF solution
(see reference)
Muscle
Rabbit
Rabbit, white New Zealand, adult, male, 2 Kg
Ives, H. , Schultz, G. , Galardy, R. and Jamieson, J.
Preparation of Functional Smooth Muscle Cells from the Rabbit Aorta ,
J Exp Med
 148 , 1400 , 1978
Cells
Smooth muscle, aorta
Enzymes
Trypsin : 0.1%
Medium
Krebs Ringer HEPES solution
Reproductive
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Reproductive
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Reproductive
Porcine
Porcine, 3-4 week
Mather, J. , Saez, J. and Haour, F.
Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells ,
Endocrinology
 110 , 933 , 1982
Cells
Leydig
Testis
Enzymes
Trypsin : 0.0003%
Medium
Lebovitz L-15 Medium
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white, mature, 3-4 Kg
Boulet, A. and Fortier, M.
Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment ,
In Vitro Cell Dev Biol
 23 , 93 , 1987
Cells
Myometrial
Enzymes
Trypsin : 0.02%, 0.03%, 0.0375%
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Nicosia, S. , Johnson, J. and Streibel, E.
Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) ,
Int J Gynecol Pathol
 3 , 348 , 1984
Cells
Mesothelial and surface epithelial
Ovaries
Enzymes
Trypsin : 0.125%-0.5%
Medium
Medium 199
Skin
Rabbit

Rabbit, Leporidae

Santos, F. , Carvalho, C. , Almeida, I. , Fagulha, T. , Rammos, F. , Barros, S. , Henriques, M. , Luís, T. and Duarte, M.
Simple Method for Establishing Primary Leporidae Skin Fibroblast Cultures. ,
Cells
 10 , 2021
Cells
Fibroblast
Enzymes
Trypsin : 0.25%
Medium
DMEM/HAMSF12
Stem
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Stem
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.