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Tissue References Search Tool

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Cartilage
Bovine
Steers, 1-2 years
Mackintosh, D. and Mason, R.
Pharmacological Actions of 17 Beta-oestradiol on Articular Cartilage Chondrocytes and Chondrosarcoma Chondrocytes in the Absence of Oestrogen Receptors ,
Biochim Biophys Acta
 964 , 295 , 1988
Cells
Chondrocytes
Enzymes
Trypsin : 0.20%
Medium
HBSS
Cartilage
Canine
Canine
Lee, J. , Kim, H. , Kim, J. , Bae, S. , Joo, D. , Huh, K. , Fang, Y. , Jeong, J. , Kim, M. and Kim, Y.
Microencapsulation of Pancreatic Islets with Canine Ear Cartilage for Immunoisolation. ,
Transplant Proc
 44 , 1091-4 , 2012
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F12
Cartilage
Chicken
Chick embryos, HH stage
Wong, M. and Tuan, R.
Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture ,
In Vitro Cell Dev Biol
 29A , 917 , 1993
Cells
Mesenchymal
Enzymes
Trypsin : 0.1%
Medium
DMEM
(see reference)
Cartilage
Chicken
Chicken, broiler strain, 8-10 weeks
Genge, B. , Wu, L. and Wuthier, R.
Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins ,
J Biol Chem
 265 , 4703 , 1990
Cells
Matrix vesicles
Epiphyseal growth plate
Enzymes
Trypsin : 0.1%
Medium
Tris-buffered saline
Cartilage
Chicken
Chicken, broiler strain, 8-10 week
Genge, B.R. , Wu, L.N.Y. and Wuthier, R.E.
Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles ,
J Biol Chem
 264 , 10917 , 1989
Cells
Matrix vesicles
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Cartilage
Chicken
Chick embryos, White Leghorn, 19 day old
Gionti, E. , Capasso, O. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro ,
J Biol Chem
 258 (11) , 7190 , 1983
Cells
Fibroblasts
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
E 199 medium
Cartilage
Chicken
Chick embryos, 19 day old
Capasso, O. , Gionti, E. , Pontarelli, G. , Ambesi-Impiobato, F. , Nitsch, L. , Tajana, G. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro ,
Exp Cell Res
 142 , 197 , 1982
Cells
Chondrocytes
Enzymes
Trypsin : 0.75%
Medium
Coon's modified F-12
Cartilage
Chicken
Chick embryos, White Leghorn
Ahrens, P. , Solursh, M. and Reiter, R.
Stage-Related Capacity for Limb Chondrogenesis in Cell Culture ,
Dev Biol
 60 , 69 , 1977
Cells
Wing buds
Enzymes
Trypsin : 0.1%
Medium
Saline G
Cartilage
Human
Human
Muraglia, A. , Nguyen, V. , Nardini, M. , Mogni, M. , Coviello, D. , Dozin, B. , Strada, P. , Baldelli, I. , Formica, M. , Cancedda, R. and Mastrogiacomo, M.
Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support. ,
Front Bioeng Biotechnol
 5 , 66 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Hyaluronidase : 0.1%
Collagenase Type 2 : 1000 u/ml
Collagenase Type 1 : 400 u/ml
Medium
Ham's F12
Cartilage
Human
Human w/ rheumatoid arthritis
Dayer, J. , Krane, S. , Russell, R. and Robinson, D.
Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells ,
Proc Natl Acad Sci U S A
 73 (3) , 945 , 1976
Cells
Synovial tissue
Enzymes
Trypsin : 0.05%
Medium
DMEM
(see reference)
Cartilage
Human
Human, 13-62 years
Srivastava, V.M.L. , Malemud, C.J. , Hough, A.J. , Bland, J.H. and Sokoloff, L.
Preliminary Experience with Cell Culture of Human Articular Chondrocytes ,
Arthritis Rheum
 17 , 165 , 1974
Cells
Articular chondrocytes
Enzymes
Trypsin : 0.2%
Medium
BSS
Cartilage
Mouse
Mouse, 2-5 day
Otsuru, S. , Hofmann, T. , Raman, P. , Olson, T. , Guess, A. , Dominici, M. and Horwitz, E.
Genomic and Functional Comparison of Mesenchymal Stromal Cells Prepared Using Two Isolation Methods. ,
Cytotherapy
 17 , 262-70 , 2015
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Collagenase Type 1 : 86.5 u/ml
Medium
DMEM
Cartilage
Rabbit
Rabbit, New Zealand, 1.8-2.3kg
Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.
Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion ,
Ann Rheum Dis
 56 , 108 , 1997
Cells
Chondrocytes
Enzymes
Medium
Gey's solution
Cartilage
Rabbit
Rabbit, white, male, 8 weeks
Benya, P.D. , Padilla, S.R. and Nimni, M.E.
The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II ,
Biochemistry
 16 , 865 , 1977
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Gey's BSS
Cartilage
Rabbit
Rabbit, New Zealand white or Dutch, 1 week
(also human, newborn)
Schindler, F.H. , Ose, M.A. and Solursh, M.
The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture ,
In Vitro
 12 , 44 , 1976
Cells
Chondrocytes
Enzymes
Trypsin : 0.1%
Medium
Saline G, CMF
Cartilage
Rabbit
Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg
Green, J.R. and William, T.
Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting ,
Clin Orthop Relat Res
 , 237 , 1976
Cells
Articular chondrocytes
Hyaline
Enzymes
Trypsin : 0.2%
Medium
Gey's BSS
Cartilage
Rabbit
Rabbit, New Zealand white, male, 250-350 g
Bentley, G. and Greer , R.
Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits ,
Nature
 230 , 385 , 1971
Cells
Epiphyseal
Articular cartilage
Enzymes
Trypsin : 0.25%
Medium
Eagle's basal medium
Cartilage
Rat
Rat
Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.
Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
 12 , e0176107 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Cartilage
Rat
Rat, SD, young, 100-120 g
Shimomura, Y. , Yoneda, T. and Suzuki, F.
Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
 19 , 179 , 1975
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Ham's F-12 medium
Epithelial
Fish
Winter flounder, 200-500 g
(Pseudopleuronectes americanus)
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Epithelial
Hamster
Hamster, Syrian golden (strain CR:RGH)
McDowell, E. and et al.
Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters ,
Am J Pathol
 129 , 511 , 1987
Cells
Tracheal
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12
Epithelial
Hamster
Hamster (strain 1516 EHS and Lakeview), 8-12 weeks
Lee, T. , Wu, R. , Brody, A. , Barrett, J. and Nettesheim, P.
Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture ,
Exp Lung Res
 6 , 27 , 1984
Cells
Tracheal
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Epithelial
Hamster
Hamster, Syrian gold, male, 6 weeks- 4 months
Goldman, W.E. and Baseman, J.B.
Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea ,
In Vitro
 16 , 313 , 1980
Cells
Tracheal
Enzymes
Trypsin : 0.25%
Medium
PBS with EDTA
Epithelial
Human
Human
Halbert CL, Alexander IE, Wolgamot GM, Miller AD.
Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells ,
J Virol
 69 , 1473-9 , 1995
Cells
Nasal polyp epithelial
Enzymes
Trypsin : 0.1%
Medium
see reference
Epithelial
Human
Human
Sabatini, L. , Allen-Hoffmann, B, Warner, T. and Azen, E.
Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands ,
In Vitro Cell Dev Biol
 27A , 939 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Epithelial
Human
Human
Robinson, C. and Wu, R.
Culture of Conducting Airway Epithelial Cells in Serum-Free Medium ,
J Tiss Cul Meth
 13 , 95 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.2%
Medium
MEM, PBS
Epithelial
Human
Human
(also bovine)
Munson, L. , Chandler, S. and Schlafer, D.
Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells ,
J Tiss Cul Meth
 11 (3) , 129 , 1988
Cells
Endometrial epithelial
Medium
DMEM/Ham's F-12
Epithelial
Human
Human, women, 27-49 years
Auersperg, N. , Siemens, C.H. and Myrdal, S.E.
Human Ovarian Surface Epithelium In Primary Culture ,
In Vitro
 20 , 743 , 1984
Cells
Epithelial
Ovary
Enzymes
Trypsin : 0.125%
Medium
HBSS, CMF
Epithelial
Human
Human, infant and neonate
Lechner, J. , Babcock, M. , Marnell, M. , Narayan, K. and Kaighn, M.
Normal Human Prostate Epithelial Cell Cultures ,
Methods Cell Biol
 21 , 195 , 1980
Cells
Epithelial
Prostate
Enzymes
Trypsin : 0.1%
Medium
HBSS
Epithelial
Mouse
Mouse
Katayama, M. and Kan, M.
Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation ,
In Vitro Cell Dev Biol
 27 , 533 , 1991
Cells
Esophageal
Enzymes
Trypsin : 0.25%
Medium
PBS, CMF
Epithelial
Mouse
Mouse, mature, female, 6-8-wk-old
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
 27A , 713 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
HBSS
Epithelial
Mouse
Mouse, female, 18 - 20 days
(also 20 - 22 days)
Fukamachi, H. and McLachlan, J.
Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum ,
In Vitro Cell Dev Biol
 27A , 907 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.25%
Medium
HBSS
Epithelial
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Nicosia, S. , Johnson, J. and Streibel, E.
Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) ,
Int J Gynecol Pathol
 3 , 348 , 1984
Cells
Mesothelial and surface epithelial
Ovaries
Enzymes
Trypsin : 0.125%-0.5%
Medium
Medium 199
Epithelial
Rat
Rat, SD, adult, male, 8-10 weeks old
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Epithelial
Rat
Rodent, various (see reference)
Robinson, C. and Wu, R.
Culture of Conducting Airway Epithelial Cells in Serum-Free Medium ,
J Tiss Cul Meth
 13 , 95 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.2%
Medium
MEM, PBS
Epithelial
Rat
Rat, Wistar, neonatal
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.