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Tissue References Search Tool

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Endothelial
Bovine
Calf
Vender, R.
Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension ,
In Vitro Cell Dev Biol
 28A , 403 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
HEPES
Endothelial
Bovine
Bovine
DeNucci, G. , Gryglewski, R.J. , Warner, T.D. and Vane, J.R.
Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled ,
Proc Natl Acad Sci U S A
 85 , 2334 , 1988
Cells
Endothelial
Aortic
Enzymes
Trypsin : 0.05%
Medium
Krebs Ringer solution
Endothelial
Bovine
Bovine
Scott, D. , Murray, J. and Barnes, M.
Investigation of the Attachment of Bovine Corneal Endothelial Cells ,
Exp Cell Res
 144 , 472 , 1983
Cells
Endothelial
Corneal
Enzymes
Trypsin : 0.2%
Medium
PBS: DMEM
Endothelial
Bovine
Bovine
Robinson, J. and Gospodarowicz, D.
Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells ,
J Cell Physiol
 117 , 368 , 1983
Cells
Endothelial, Corneal
Enzymes
Trypsin : 0.05%
Medium
0.01M Phosphate buffer with 0.02% EDTA
0.9% NaCl (See Reference)
Endothelial
Canine
Dog, mongrel, adult
Ford, J. , Burkel, W. and Kahn, R.
Isolation of Adult Canine Venous Endothelium for Tissue Culture ,
In Vitro
 17 , 44 , 1981
Cells
Endothelium
Jugular vein
Enzymes
Trypsin : 0.1%
Medium
Earle's PBS, CMF
Endothelial
Human
Human
Goolcharran, C. , Cleland, J. , Keck, R. , Jones, A. and Borchardt, R.
Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides ,
AAPS PharmSci
 2 (1) , 5 , 2000
Cells
Endothelial
Enzymes
Trypsin : 2%
Medium
PBS
Endothelial
Human
Human
Wojta J, Gallicchio M, Zoellner H, Filonzi E, Hamilton J, McGrath K.
Interleukin-4 stimulates expression of urokinase-type-plasminogen activator in cultured human foreskin microvascular endothelial cells ,
Blood
 81 , 3285-92 , 1993
Cells
Foreskin microvascular endothelial
Enzymes
Trypsin : 0.3%
Medium
HBSS
Endothelial
Human
Human
Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ.
Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis ,
Am J Physiol
 262 , H1088-95 , 1992
Cells
Vascular endothelial
Enzymes
Trypsin : 0.25%
Medium
M-199
Endothelial
Human
Human
Lee, K, Lawley, T, Xu, Y. and Swerlick, R.
VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells ,
J Invest Dermatol
 98 , 79 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.3.%
Medium
HBSS
(see reference)
Endothelial
Human
Human
Kubota, Y. , Kleinman, H. , Martin, G. and Lawley, T.
Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures ,
J Cell Biol
 107 , 1589 , 1988
Cells
Endothelial/HUVEC
Foreskin & umbilical cord
Enzymes
Trypsin : 0.3%
Medium
HBSS/PBS, Medium 199
(see reference)
Endothelial
Human
Human
Marks, R.M. , Czerniecki, M. and Penny, R.
Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture ,
In Vitro Cell Dev Biol
 21 , 627 , 1985
Cells
Endothelial
Dermal
Enzymes
Trypsin : 0.3%
Medium
PBS
Endothelial
Human
Human
Jaffe, E.A. , Minick, C.R. , Adelman, B. , Becker, C.G. and Nachman, R.
Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells ,
J Exp Med
 144 , 209 , 1976
Cells
Umbilical vein
Enzymes
Trypsin : 100 µg/ml
Medium
Tris-HCl,0.2 M
Endothelial
Porcine
Porcine
Shasby, S.
Endothelial Cells Grown On Permeable Membrane Supports ,
J Tiss Cul Meth
 14 , 247 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
Medium 199
Endothelial
Porcine
Porcine
Vischer, P. and Buddecke, E.
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells ,
Exp Cell Res
 158 , 15 , 1985
Cells
Endothelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Porcine
Porcine, 60-100 days
Coulson, W.F.
The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin ,
Biochim Biophys Acta
 237 , 378 , 1971
Cells
Aorta
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer
(see reference)
Endothelial
Rat
Rat, 300 G, and pig, 30-40 Kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS
Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Chicken
Chick embryo
(also rat)
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Eye
Fish

Zebrafish, Danio rerio

Middel, C. , Dietrich, N. , Hammes, H. and Kroll, J.
Analysis of the Morphology of Retinal Vascular Cells in Zebrafish , Front Cell Dev Biol Vol. 11 , 1267232 , 2023
Cells
Retinal vascular
Enzymes
Trypsin : 0.3%
Medium
PBS
Eye
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Eye
Mouse
Mouse, 4-8 week
Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y.
New Technique for Culturing Corneal Epithelial Cells of Normal Mice. ,
Mol Vis
 15 , 1589-93 , 2009
Cells
Corneal epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Eye
Mouse
Mouse, adult
Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
 24 , 2714-22 , 2006
Cells
Stromal
Enzymes
Trypsin : 0.05%
Collagenase : 78 u/ml
Hyaluronidase : 38 u/ml
Medium
DMEM/F-12
Eye
Rabbit
Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.
Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
 45 , 807-12 , 2004
Cells
Corneal keratocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.5%
Medium
PBS
Eye
Rabbit
Rabbit, New Zealand, adult, male, albino,
2 kg
Johnson-Muller, B. and Gross, J.
Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions ,
Proc Natl Acad Sci U S A
 75 (9) , 4417 , 1978
Cells
Epithelial
Enzymes
Trypsin : 0.25%
Medium
HBSS/DMEM
Eye
Rat
Rat, embryonic 17 day
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.