Tissue References Search Tool

Chicken

Chick, embryo, 6 day

Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.

Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
180 , 159-68 , 2005

Cells

Retinal cells

Enzymes

Trypsin : 0.005%

Deoxyribonuclease I : 0.005%

Medium

DMEM/F12

Eye

Chicken

Chick embryo

Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.

ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
43 , 3181-9 , 2002

Cells

Corneal epithelia

Enzymes

Collagenase : 0.08%

Trypsin : 0.08%

Medium

HBSS

Eye

Chicken

Chick embryo
(also rat)

Seigel, G.

The Golden Age of Retinal Cell Culture ,
Mol Vis
5 , 4 , 1999

Cells

Retinal

Enzymes

Trypsin : 0.6%

Medium

DMEM

Eye

Chicken

Chick, embryo, 10-14 day

Moyer, M. , Bullrich, F. and Sheffield, J.

Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
26 , 1073 , 1990

Cells

Flat, retina

Enzymes

Trypsin : 0.1%

Medium

Tyrode's solution, CMF

Eye

Fish

Zebrafish, Danio rerio

Middel, C. , Dietrich, N. , Hammes, H. and Kroll, J.

Analysis of the Morphology of Retinal Vascular Cells in Zebrafish , Front Cell Dev Biol Vol. 11 , 1267232 , 2023

Cells

Retinal vascular

Enzymes

Trypsin : 0.3%

Medium

PBS

Eye

Human

Human, 5-65 years

Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.

Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
69 , 97 , 1999

Cells

Retinal pigment epithelial (RPE)

Enzymes

Trypsin : 0.25%

Medium

HBSS

Eye

Mouse

Mouse, 4-8 week

Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y.

New Technique for Culturing Corneal Epithelial Cells of Normal Mice. ,
Mol Vis
15 , 1589-93 , 2009

Cells

Corneal epithelial

Enzymes

Neutral Protease : 1.5%

Trypsin : 0.25%

Medium

DMEM/F12

Eye

Mouse

Mouse, adult

Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.

Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
24 , 2714-22 , 2006

Cells

Stromal

Enzymes

Trypsin : 0.05%

Collagenase : 78 u/ml

Hyaluronidase : 38 u/ml

Medium

DMEM/F-12

Eye

Rabbit

Rabbit, New Zealand

Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.

Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
45 , 807-12 , 2004

Cells

Corneal keratocytes

Enzymes

Trypsin : 0.25%

Collagenase : 0.5%

Medium

PBS

Eye

Rabbit

Rabbit, New Zealand, adult, male, albino,
2 kg

Johnson-Muller, B. and Gross, J.

Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions ,
Proc Natl Acad Sci U S A
75 (9) , 4417 , 1978

Cells

Epithelial

Enzymes

Trypsin : 0.25%

Medium

HBSS/DMEM

Eye

Rat

Rat, embryonic 17 day

Seigel, G.

The Golden Age of Retinal Cell Culture ,
Mol Vis
5 , 4 , 1999

Cells

Retinal

Enzymes

Trypsin : 0.6%

Medium

DMEM

Eye

Rat

Rat, SD, pups

Sarthy PV, Curtis BM. and Catterall WA.

Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
3 (12) , 2532 , 1983

Cells

Retina

Enzymes

Trypsin : 0.25%

Medium

Ham's F-12

Kidney

Fish

Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g

Dickman, K. and Renfro, J.

Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
251 , 424 , 1986

Cells

Renal tubule

Enzymes

Trypsin : 0.2%

Medium

CMF solution

Kidney

Guinea-Pig

Guinea-pig, male, 100 g

Phillips, H.

Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
8 , 101 , 1972

Cells

Single cells

Enzymes

Trypsin : 0.25%

Medium

CF salt solution

Kidney

Human

McAteer, J, Kempson, S. and Evan, A

Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
13 , 143 , 1991

Cells

Renal cortex

Enzymes

Trypsin : 0.1%

Medium

Tissue Culture Grade Water

Kidney

Human

Heieren, M. , van der Woude, F. and Balfour Jr., H.

Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
85 , 1642 , 1988

Cells

Mesangial

Enzymes

Trypsin : 0.25%

Medium

DMEM/Ham's F-12

Kidney

Human

Human, 24 newborn and stillborn
(also rabbit)

De Oca, H. and Malinin, T.

Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
2 , 243 , 1975

Cells

Renal

Enzymes

Trypsin : 0.25%

Medium

See reference

Kidney

Monkey

Melnick, J. and Wallis, C.

Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells ,
Dev Biol
37 , 77 , 1976

Cells

Kidney

Enzymes

Trypsin : 0.01% - 0.00001%

Medium

Eagle's MEM

Kidney

Mouse

Mouse, 3-7 month

Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.

Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
10 , 1569-79 , 1999

Cells

Fibroblasts, mesangial, smooth muscle

Enzymes

Trypsin : 0.25%

Collagenase : see reference

Soybean Trypsin Inhibitor : 0.05%

Medium

DMEM

Kidney

Rabbit

Rabbit, New Zealand, white

Grenier, F. and Smith, W.

Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae ,
Prostaglandins
16 , 759 , 1978

Cells

Collecting tubule

Enzymes

Trypsin : 0.05%

Medium

Kreb's Ringer buffer

Kidney

Rat

Wallach, D. , Anderson, W. and Pastan, I.

Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin ,
J Biol Chem
253 , 24 , 1978

Cells

Fibroblasts

Kidney

Enzymes

Trypsin : up to 0.25%

Medium

Dulbecco-Vogt MEM

Tissue Tables (references, grouped by tissue type and species)

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Tissue References Search Tool

Tissue Tables (references, grouped by tissue type and species)