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Tissue References Search Tool

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Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Chicken
Chick embryo
(also rat)
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Eye
Fish

Zebrafish, Danio rerio

Middel, C. , Dietrich, N. , Hammes, H. and Kroll, J.
Analysis of the Morphology of Retinal Vascular Cells in Zebrafish , Front Cell Dev Biol Vol. 11 , 1267232 , 2023
Cells
Retinal vascular
Enzymes
Trypsin : 0.3%
Medium
PBS
Eye
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Eye
Mouse
Mouse, 4-8 week
Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y.
New Technique for Culturing Corneal Epithelial Cells of Normal Mice. ,
Mol Vis
 15 , 1589-93 , 2009
Cells
Corneal epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Eye
Mouse
Mouse, adult
Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
 24 , 2714-22 , 2006
Cells
Stromal
Enzymes
Trypsin : 0.05%
Collagenase : 78 u/ml
Hyaluronidase : 38 u/ml
Medium
DMEM/F-12
Eye
Rabbit
Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.
Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
 45 , 807-12 , 2004
Cells
Corneal keratocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.5%
Medium
PBS
Eye
Rabbit
Rabbit, New Zealand, adult, male, albino,
2 kg
Johnson-Muller, B. and Gross, J.
Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions ,
Proc Natl Acad Sci U S A
 75 (9) , 4417 , 1978
Cells
Epithelial
Enzymes
Trypsin : 0.25%
Medium
HBSS/DMEM
Eye
Rat
Rat, embryonic 17 day
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Lung
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Human
Human
Zhu, Y. , Skold, C. , Liu, X. , Wang, H. , Kohyama. , Wen, F. , Ertl, R. and Rennard, S.
Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture ,
Respir Res
 2 (5) , 295 , 2001
Cells
Lung
Fibroblasts
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Human
Human, 16-24 week therapeutic abortions
Liley, H. , Ertsey, R. , Gonzales, L. , Odom, M. , Hawgood, S. , Dobbs, L. and Ballard, P.
Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung ,
Biochim Biophys Acta
 961 , 86 , 1988
Cells
Alveolar type II
Fetal
Enzymes
Trypsin : 50 ug/ml
Medium
Ham's F-12, Eagle's MEM
Lung
Human
Human
Lechner, J. , Haugen, A. , McClendon, I. and Pettis, E.
Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium ,
In Vitro
 18 (7) , 633 , 1982
Cells
Epithelial
Enzymes
Trypsin : 0.02%
Medium
Medium 199
Lung
Human
Human, 12-16 wk old embryo
Kan, M. and Yamane, I.
In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium ,
J Cell Physiol
 111 , 155 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.01%
Medium
Eagle's MEM
Lung
Mouse
Mouse, 6 week
Lewis, K. , Hall, J. , Kiyotake, E. , Christensen, T. , Balasubramaniam, V. and Anseth, K.
Epithelial-Mesenchymal Crosstalk Influences Cellular Behavior in a 3D Alveolus-Fibroblast Model System. ,
Biomaterials
 155 , 124 , 2018
Cells
Alveolar epithelial
Enzymes
Medium
DMEM/F-12
Lung
Mouse
Mouse, embryo, day 18
Trotter, A. , Kipp, M. , Schrader, R. and Beyer, C.
Combined Application of 17Beta-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice. ,
Int J Pediatr
 2009 , 170491 , 2009
Cells
Alveolar and fibroblast
Enzymes
Trypsin : 2.5%
Collagenase Type 1 : 1250 u/ml
Medium
MEM
Lung
Rabbit
Rabbit, New Zealand, white
Scott, J.
The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung ,
Exp Lung Res
 12 , 181 , 1987
Cells
Alveolar type II
Enzymes
Trypsin : 0.05%
Medium
HBSS
Lung
Rabbit
Rabbit, New Zealand white, adult,male
Finkelstein, J. , Maniscalco, W. and Shapiro, D.
Properties of Freshly Isolated Type II Alveolar Epithelial Cells ,
Biochim Biophys Acta
 762 , 398 , 1983
Cells
Alveolar type II
Enzymes
Trypsin : 0.0025%
Medium
Joklik's MEM
Lung
Rat
Rat, embryonic day 15
Fraslon-Vanhulle, C, Bourbon, J. and Batenburg, J
Culture of Fetal Alveolar Epithelial Type II Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 13A:2.1 , 1995
Cells
Fetal alveolar epithelial type II
Enzymes
Trypsin : 0.1%
Medium
DMEM
Lung
Rat
Rat, Wistar, female, virgin
Fraslon, C. , Rolland, G. , Bourbon, J. , Rieutort, M. and Valenza, C.
Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 843 , 1991
Cells
Alveolar epithelial type II
Enzymes
Trypsin : 1%
Medium
Eagle's MEM
Lung
Rat
Rat, Wistar
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS
Lung
Rat
Rat, SD, 8 day
Berk, J. , Franzblau, C. and Goldstein, R.
Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype ,
J Biol Chem
 266 , 3192 , 1991
Cells
Interstitial
Enzymes
Trypsin : 1.125%
Medium
HEPES buffer
Lung
Rat
Rat, Wistar, adult, male and pregnant female (known gestation)
Batenburg, J. , Otto-Verberne, C. , Have-Opbroek, A. and Klazinga, W.
Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture ,
Biochim Biophys Acta
 960 , 441 , 1988
Cells
Alveolar type II
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640
Lung
Rat
Rat, SD, male, 150 - 200 g
Brown, L. and Longmore, W.
Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats ,
Biochim Biophys Acta
 878 , 258 , 1986
Cells
Pneumocytes type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, SD, male
Weller, N. and Karnovksy, M.
Isolation of Pulmonary Alveolar Type I Cells From Adult Rats ,
Am J Pathol
 124 , 448 , 1986
Cells
Alveolar type I
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Rat
Rat, fetus, 19 day
Post, M. , Torday, J. and Smith, B.
Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor ,
Exp Lung Res
 7 , 53 , 1984
Cells
Fibroblasts & type II
Enzymes
Trypsin : 0.05%
Medium
HBSS: serum free MEM, CMF
Lung
Rat
Rat, SD, male/female, 180-250 g
Mason, R. , Williams, M. , Greenleaf, R. and Clements, J.
Isolation and Properties of Type II Alveolar Cells from Rat Lung ,
Am Rev Respir Dis
 115 , 1015 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, Wistar, pathogen free
King, R.
Metabolic Fate of the Apoproteins of Pulmonary Surfactant ,
Am Rev Respir Dis
 115 , 73 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.50%
Medium
Earle's MEM
Lung
Rat
Rat, fetus, 19 days
Douglas, W. and Teel, R.
An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes ,
Am Rev Respir Dis
 113 , 17 , 1976
Cells
Alveolar pneumonocytes, type II
Enzymes
Trypsin : 0.1 %
Medium
HBSS, CMF
Lung
Rat
Rat, SD, male, 100 g
Kikkawa, Y. and Yoneda, K.
Type II Epithelial Cell of the Lung. I. Method of Isolation ,
Lab Invest
 30 , 76 , 1974
Cells
Alveolar type II
Enzymes
Trypsin : 1.0%
Medium
Joklik's medium

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.