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Tissue References Search Tool

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Lung
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Human
Human
Zhu, Y. , Skold, C. , Liu, X. , Wang, H. , Kohyama. , Wen, F. , Ertl, R. and Rennard, S.
Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture ,
Respir Res
 2 (5) , 295 , 2001
Cells
Lung
Fibroblasts
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Human
Human, 16-24 week therapeutic abortions
Liley, H. , Ertsey, R. , Gonzales, L. , Odom, M. , Hawgood, S. , Dobbs, L. and Ballard, P.
Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung ,
Biochim Biophys Acta
 961 , 86 , 1988
Cells
Alveolar type II
Fetal
Enzymes
Trypsin : 50 ug/ml
Medium
Ham's F-12, Eagle's MEM
Lung
Human
Human
Lechner, J. , Haugen, A. , McClendon, I. and Pettis, E.
Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium ,
In Vitro
 18 (7) , 633 , 1982
Cells
Epithelial
Enzymes
Trypsin : 0.02%
Medium
Medium 199
Lung
Human
Human, 12-16 wk old embryo
Kan, M. and Yamane, I.
In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium ,
J Cell Physiol
 111 , 155 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.01%
Medium
Eagle's MEM
Lung
Mouse
Mouse, 6 week
Lewis, K. , Hall, J. , Kiyotake, E. , Christensen, T. , Balasubramaniam, V. and Anseth, K.
Epithelial-Mesenchymal Crosstalk Influences Cellular Behavior in a 3D Alveolus-Fibroblast Model System. ,
Biomaterials
 155 , 124 , 2018
Cells
Alveolar epithelial
Enzymes
Medium
DMEM/F-12
Lung
Mouse
Mouse, embryo, day 18
Trotter, A. , Kipp, M. , Schrader, R. and Beyer, C.
Combined Application of 17Beta-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice. ,
Int J Pediatr
 2009 , 170491 , 2009
Cells
Alveolar and fibroblast
Enzymes
Trypsin : 2.5%
Collagenase Type 1 : 1250 u/ml
Medium
MEM
Lung
Rabbit
Rabbit, New Zealand, white
Scott, J.
The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung ,
Exp Lung Res
 12 , 181 , 1987
Cells
Alveolar type II
Enzymes
Trypsin : 0.05%
Medium
HBSS
Lung
Rabbit
Rabbit, New Zealand white, adult,male
Finkelstein, J. , Maniscalco, W. and Shapiro, D.
Properties of Freshly Isolated Type II Alveolar Epithelial Cells ,
Biochim Biophys Acta
 762 , 398 , 1983
Cells
Alveolar type II
Enzymes
Trypsin : 0.0025%
Medium
Joklik's MEM
Lung
Rat
Rat, embryonic day 15
Fraslon-Vanhulle, C, Bourbon, J. and Batenburg, J
Culture of Fetal Alveolar Epithelial Type II Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 13A:2.1 , 1995
Cells
Fetal alveolar epithelial type II
Enzymes
Trypsin : 0.1%
Medium
DMEM
Lung
Rat
Rat, Wistar, female, virgin
Fraslon, C. , Rolland, G. , Bourbon, J. , Rieutort, M. and Valenza, C.
Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 843 , 1991
Cells
Alveolar epithelial type II
Enzymes
Trypsin : 1%
Medium
Eagle's MEM
Lung
Rat
Rat, Wistar
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS
Lung
Rat
Rat, SD, 8 day
Berk, J. , Franzblau, C. and Goldstein, R.
Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype ,
J Biol Chem
 266 , 3192 , 1991
Cells
Interstitial
Enzymes
Trypsin : 1.125%
Medium
HEPES buffer
Lung
Rat
Rat, Wistar, adult, male and pregnant female (known gestation)
Batenburg, J. , Otto-Verberne, C. , Have-Opbroek, A. and Klazinga, W.
Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture ,
Biochim Biophys Acta
 960 , 441 , 1988
Cells
Alveolar type II
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640
Lung
Rat
Rat, SD, male, 150 - 200 g
Brown, L. and Longmore, W.
Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats ,
Biochim Biophys Acta
 878 , 258 , 1986
Cells
Pneumocytes type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, SD, male
Weller, N. and Karnovksy, M.
Isolation of Pulmonary Alveolar Type I Cells From Adult Rats ,
Am J Pathol
 124 , 448 , 1986
Cells
Alveolar type I
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Rat
Rat, fetus, 19 day
Post, M. , Torday, J. and Smith, B.
Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor ,
Exp Lung Res
 7 , 53 , 1984
Cells
Fibroblasts & type II
Enzymes
Trypsin : 0.05%
Medium
HBSS: serum free MEM, CMF
Lung
Rat
Rat, SD, male/female, 180-250 g
Mason, R. , Williams, M. , Greenleaf, R. and Clements, J.
Isolation and Properties of Type II Alveolar Cells from Rat Lung ,
Am Rev Respir Dis
 115 , 1015 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, Wistar, pathogen free
King, R.
Metabolic Fate of the Apoproteins of Pulmonary Surfactant ,
Am Rev Respir Dis
 115 , 73 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.50%
Medium
Earle's MEM
Lung
Rat
Rat, fetus, 19 days
Douglas, W. and Teel, R.
An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes ,
Am Rev Respir Dis
 113 , 17 , 1976
Cells
Alveolar pneumonocytes, type II
Enzymes
Trypsin : 0.1 %
Medium
HBSS, CMF
Lung
Rat
Rat, SD, male, 100 g
Kikkawa, Y. and Yoneda, K.
Type II Epithelial Cell of the Lung. I. Method of Isolation ,
Lab Invest
 30 , 76 , 1974
Cells
Alveolar type II
Enzymes
Trypsin : 1.0%
Medium
Joklik's medium
Skin
Frog
Frog
Nishikawa, A. , Shimizu-Nishikawa, K. and Miler, L.
Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis ,
In Vitro Cell Dev Biol
 26 , 1128 , 1990
Cells
Epidermal
Enzymes
Trypsin : 0.18%
Medium
Barth's solution, CMF
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human, adult
Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A.
Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. ,
PLoS ONE
 8 , e78365 , 2013
Cells
Epidermal stem cells
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 2.5%
Medium
DMEM
Skin
Human
Human, female, 18-66 yr
Huschtscha, L. , Napier, C. , Noble, J. , Bower, K. , Au, A. , Campbell, H. , Braithwaite, A. and Reddel, R.
Enhanced Isolation of Fibroblasts From Human Skin Explants. ,
Biotechniques
 53 , 239 , 2012
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
DMEM
Skin
Human
Human
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei.
Isolation of a novel population of multipotent adult stem cells from human hair follicles ,
Am J Pathol
 168 , 1879-88 , 2006
Cells
Hair follicular epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Skin
Human
Human
Clark, R. , Chong, B. , Mirchandani, N. , Yamanaka, K. , Murphy, G. , Dowgiert, R. and Kupper, T.
A Novel Method for the Isolation of Skin Resident T cells from Normal and Diseased Human Skin. ,
J Invest Dermatol
 126 , 1059 , 2006
Cells
fibroblasts
Enzymes
Collagenase : 0.5%
Trypsin : 0.25%
Medium
DMEM/F12
Skin
Human
Human
Tuan Tai-Lan, Wu Huayang, Huang Eunice Y, Chong Sheree S N, Laug Walter, Messadi Diana, Kelly Paul, Le Anh.
Increased plasminogen activator inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures ,
Am J Pathol
 162 , 1579-89 , 2003
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human
Hybbinette S, Bostrom M, Lindberg K.
Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation ,
Exp Dermatol
 8 , 30-8 , 1999
Cells
Keratinocytes
Enzymes
Trypsin : 0.17%
Thermolysin : 0.05%
Medium
see reference
Skin
Human
Human
Judd, D. , Battista, P. and Behm, D.
Culture of Human Keratinocytes in Defined Serum-Free Medium ,
Focus
 19 (1) , 2 , 1997
Cells
Keratinocytes
Enzymes
Trypsin : 0.05%
Medium
Dulbecco's PBS
Skin
Human
Human
Harley, C. and Sherwood, S.
, Methods in Molecular Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75 , Pollard, J., and Walker, J. , Humana Press , 23 , 1997
Cells
Epidermis plus dermis
Abdomen or inner forearm
Enzymes
Trypsin : 0.125%
Medium
DMEM, M199
Skin
Human
Human
Regnier, M.
Culture of Human Karatinocytes , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 11B:4.1 , 1995
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, adult, female, breast skin
Hirel, B. , Chesne, C. , Pailgheret, J. and Guillouzo, A.
Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions ,
In Vitro Cell Dev Biol
 30A , 372 , 1994
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human, neonatal foreskin
Hansbrough, J. , Cooper, M. , Cohen, R. , Spielvogel, R. , Greenleaf, G. , Bartel, R. and Naughton, G.
Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice ,
Surgery
 111 (1) , 438 , 1992
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human
Limat, A. , Hunziker, T. , Boillat, C. , Noser, F. and Wiesmann, U.
Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation ,
In Vitro Cell Dev Biol
 26 , 709 , 1990
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
CMF solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.