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Lung
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Human
Human
Zhu, Y. , Skold, C. , Liu, X. , Wang, H. , Kohyama. , Wen, F. , Ertl, R. and Rennard, S.
Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture ,
Respir Res
 2 (5) , 295 , 2001
Cells
Lung
Fibroblasts
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Human
Human, 16-24 week therapeutic abortions
Liley, H. , Ertsey, R. , Gonzales, L. , Odom, M. , Hawgood, S. , Dobbs, L. and Ballard, P.
Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung ,
Biochim Biophys Acta
 961 , 86 , 1988
Cells
Alveolar type II
Fetal
Enzymes
Trypsin : 50 ug/ml
Medium
Ham's F-12, Eagle's MEM
Lung
Human
Human
Lechner, J. , Haugen, A. , McClendon, I. and Pettis, E.
Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium ,
In Vitro
 18 (7) , 633 , 1982
Cells
Epithelial
Enzymes
Trypsin : 0.02%
Medium
Medium 199
Lung
Human
Human, 12-16 wk old embryo
Kan, M. and Yamane, I.
In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium ,
J Cell Physiol
 111 , 155 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.01%
Medium
Eagle's MEM
Lung
Mouse
Mouse, 6 week
Lewis, K. , Hall, J. , Kiyotake, E. , Christensen, T. , Balasubramaniam, V. and Anseth, K.
Epithelial-Mesenchymal Crosstalk Influences Cellular Behavior in a 3D Alveolus-Fibroblast Model System. ,
Biomaterials
 155 , 124 , 2018
Cells
Alveolar epithelial
Enzymes
Medium
DMEM/F-12
Lung
Mouse
Mouse, embryo, day 18
Trotter, A. , Kipp, M. , Schrader, R. and Beyer, C.
Combined Application of 17Beta-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice. ,
Int J Pediatr
 2009 , 170491 , 2009
Cells
Alveolar and fibroblast
Enzymes
Trypsin : 2.5%
Collagenase Type 1 : 1250 u/ml
Medium
MEM
Lung
Rabbit
Rabbit, New Zealand, white
Scott, J.
The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung ,
Exp Lung Res
 12 , 181 , 1987
Cells
Alveolar type II
Enzymes
Trypsin : 0.05%
Medium
HBSS
Lung
Rabbit
Rabbit, New Zealand white, adult,male
Finkelstein, J. , Maniscalco, W. and Shapiro, D.
Properties of Freshly Isolated Type II Alveolar Epithelial Cells ,
Biochim Biophys Acta
 762 , 398 , 1983
Cells
Alveolar type II
Enzymes
Trypsin : 0.0025%
Medium
Joklik's MEM
Lung
Rat
Rat, embryonic day 15
Fraslon-Vanhulle, C, Bourbon, J. and Batenburg, J
Culture of Fetal Alveolar Epithelial Type II Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 13A:2.1 , 1995
Cells
Fetal alveolar epithelial type II
Enzymes
Trypsin : 0.1%
Medium
DMEM
Lung
Rat
Rat, Wistar, female, virgin
Fraslon, C. , Rolland, G. , Bourbon, J. , Rieutort, M. and Valenza, C.
Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 843 , 1991
Cells
Alveolar epithelial type II
Enzymes
Trypsin : 1%
Medium
Eagle's MEM
Lung
Rat
Rat, Wistar
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS
Lung
Rat
Rat, SD, 8 day
Berk, J. , Franzblau, C. and Goldstein, R.
Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype ,
J Biol Chem
 266 , 3192 , 1991
Cells
Interstitial
Enzymes
Trypsin : 1.125%
Medium
HEPES buffer
Lung
Rat
Rat, Wistar, adult, male and pregnant female (known gestation)
Batenburg, J. , Otto-Verberne, C. , Have-Opbroek, A. and Klazinga, W.
Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture ,
Biochim Biophys Acta
 960 , 441 , 1988
Cells
Alveolar type II
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640
Lung
Rat
Rat, SD, male, 150 - 200 g
Brown, L. and Longmore, W.
Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats ,
Biochim Biophys Acta
 878 , 258 , 1986
Cells
Pneumocytes type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, SD, male
Weller, N. and Karnovksy, M.
Isolation of Pulmonary Alveolar Type I Cells From Adult Rats ,
Am J Pathol
 124 , 448 , 1986
Cells
Alveolar type I
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Rat
Rat, fetus, 19 day
Post, M. , Torday, J. and Smith, B.
Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor ,
Exp Lung Res
 7 , 53 , 1984
Cells
Fibroblasts & type II
Enzymes
Trypsin : 0.05%
Medium
HBSS: serum free MEM, CMF
Lung
Rat
Rat, SD, male/female, 180-250 g
Mason, R. , Williams, M. , Greenleaf, R. and Clements, J.
Isolation and Properties of Type II Alveolar Cells from Rat Lung ,
Am Rev Respir Dis
 115 , 1015 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, Wistar, pathogen free
King, R.
Metabolic Fate of the Apoproteins of Pulmonary Surfactant ,
Am Rev Respir Dis
 115 , 73 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.50%
Medium
Earle's MEM
Lung
Rat
Rat, fetus, 19 days
Douglas, W. and Teel, R.
An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes ,
Am Rev Respir Dis
 113 , 17 , 1976
Cells
Alveolar pneumonocytes, type II
Enzymes
Trypsin : 0.1 %
Medium
HBSS, CMF
Lung
Rat
Rat, SD, male, 100 g
Kikkawa, Y. and Yoneda, K.
Type II Epithelial Cell of the Lung. I. Method of Isolation ,
Lab Invest
 30 , 76 , 1974
Cells
Alveolar type II
Enzymes
Trypsin : 1.0%
Medium
Joklik's medium
Tumor
Human
Human
Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
 , e52532 , 2015
Cells
Colon
Enzymes
Trypsin : 0.1%
Collagenase : 0.1%
Medium
HBSS
Tumor
Human
Human
Hasselbach, L. , Irtenkauf, S. , Lemke, N. , Nelson, K. , Berezovsky, A. , Carlton, E. , Transou, A. , Mikkelsen, T. and deCarvalho, A.
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. ,
J Vis Exp
 , e51088 , 2014
Cells
Glioblastoma tumor
Enzymes
Trypsin : 0.025%
Collagenase Type 4 : 500 u/ml
Medium
HBSS
Tumor
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Tumor
Human
Human
Quintana, E. , Shackleton, M. , Foster, H. , Fullen, D. , Sabel, M. , Johnson, T. and Morrison, S.
Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized. ,
Cancer Cell
 18 , 510 , 2010
Cells
Tumorigenic melanoma
Enzymes
Collagenase Type 4 : 200 u/ml
Trypsin : 0.05%
Deoxyribonuclease I : 50-100 u/ml
Medium
PBS
Tumor
Human
Human
MacLeod, R.
Rapid Monolayer Primary Cell Culture from Tissue Biopsy , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 3E:2.1 , 1995
Cells
Epithelial, fibroblasts
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Tumor
Human
Human
Sacks, P. , Parnes, S. , Gallick, G. , Mansouri, Z. , Lichtner, R. , Satya-Prakash, K. , Pathak, S. and Parsons, D.
Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck ,
Cancer Res
 48 , 2858 , 1988
Cells
Tumor
Enzymes
Trypsin : 0.05%
Medium
DMEM
Tumor
Human
Human
Brattain, M. , Marks, M. , McCombs, J. , Finely, W. and Brattain, D.
Characterization of Human Colon Carcinoma Cell Lines Isolated From a Single Primary Tumour ,
Br J Cancer
 47 , 373 , 1983
Cells
Tumor, colon
Enzymes
Trypsin : 0.25%
Medium
McCoy's
Tumor
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
Tumor
Mouse
Mouse
Chen, X. , Stewart, E. , Shelat, A. , Qu, C. , Bahrami, A. , Hatley, M. , Wu, G. , Bradley, C. , McEvoy, J. , Pappo, A. , Spunt, S. , Valentine, M. , Valentine, V. , Krafcik, F. , Lang, W. , Wierdl, M. and Tsurkan, L.
Targeting Oxidative Stress in Embryonal Rhabdomyosarcoma. ,
Cancer Cell
 24 , 710-24 , 2013
Cells
Rhabdomycosarcoma tumor
Enzymes
Trypsin : 0.012%
Medium
DMEM
Tumor
Mouse
Mouse, BALB/cfC3H/Crgl
Hosick, H.
A Note on Growth Patterns of Epithelial Tumor Cells in Primary Culture ,
Cancer Res
 34 , 259 , 1974
Cells
Neoplastic
Epithelial tumor
Medium
DMEM
Tumor
Rat
Rat
Brennan, M. , Oldberg, A. , Hayman, E. and Ruoslahti, E.
Effect of a Proteoglycan Produced by Rat Tumor Cells on Their Adhesion to Fibronectin-Collagen Substrata ,
Cancer Res
 43 , 4302 , 1983
Cells
Yolk sac tumor
Enzymes
Trypsin : 0.01%
Medium
DMEM
Tumor
Rat
Rat
Gazdar, A. , Chick, W. , Oie, H. , Sims, H. , King, D. , Weir, G. and Lauris, V.
Continuous, Clonal, Insulin-and Somatostatin-Secreting Cell Lines Established from a Transplantable Rat Islet Cell Tumor ,
Proc Natl Acad Sci U S A
 77 (6) , 3519 , 1980
Cells
Tumor, islet
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Tumor
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.