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Tissue References Search Tool

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Endothelial
Bovine
Bovine, adult
Cotta-Pereira, G. , Sage, H. , Bornstein, P. , Ross, R. and Schwartz, S.
Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis ,
J Cell Physiol
 102 , 183 , 1980
Cells
Aorta
Enzymes
Collagenase Type 1 : 125 u/ml
Medium
Dulbecco's PBS with calcium and magnesium
Endothelial
Bovine
Bovine
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Bovine
Bovine
Eskin, S. , Sybers, H. , Trevino, L. , Lie, J. and Chimoskey, J.
Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein ,
In Vitro
 14 , 903 , 1978
Cells
Saphenous Vein
Aorta
Enzymes
Collagenase : 0.01%
Medium
PBS
Endothelial
Bovine
Bovine
Ryan, U.S. , Clements, E. , Habliston, D. and Ryan, J.W.
Isolation And Culture of Pulmonary Artery Endothelial Cells ,
Tissue Cell
 10 , 535 , 1978
Cells
Pulmonary artery
Enzymes
Medium
Puck's solution
Endothelial
Bovine
Bovine
Schwartz, S.M.
Selection and Characterization of Bovine Aortic Endothelial Cells ,
In Vitro
 14 , 966 , 1978
Cells
Endothelial
Thoracic aorta
Saphenous veins
Enzymes
Medium
PBS
Endothelial
Bovine
Calf
Howard, B. , Macarak, E. , Gunson, D. and Kefalides, N.
Characterization of the Collagen Synthesized by Endothelial Cells in Culture ,
Proc Natl Acad Sci U S A
 73 (7) , 2361 , 1976
Cells
Endothelial
Enzymes
Medium
DMEM
Epithelial
Bovine
Bovine, fetal
Schumann, B.L. , Cody, T.E. , Miller, M.L. and Leikauf, G.D.
Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells ,
In Vitro Cell Dev Biol
 24 , 211 , 1988
Cells
Epithelial
Tracheal
Enzymes
Medium
Dissociation medium, CMF
Epithelial
Chicken
Chick, 5 day old
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Eye
Bovine
Bovine
Terlizzi, V. , Kolibabka, M. , Burgess, J. , Hammes, H. and Harmsen, M.
The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2. ,
Stem Cells
 36 , 240-251 , 2018
Cells
Retinal endothelial
Enzymes
Pronase : 0.02%
Medium
DMEM
Eye
Bovine
Bovine
Bryan, B. and D'Amore, P.
Pericyte Isolation and USe is Endothelial/Pericyte Coculture Models ,
Meth Enzymol
 443 , 315 , 2008
Cells
Pericyte
Enzymes
Collagenase : 0.2%
Medium
DMEM
Eye
Bovine
Bovine
Bowman, P. , Betz, A. and Goldstein, G.
Primary Culture of Microvascular Endothelial Cells From Bovine Retina ,
In Vitro
 18 (7) , 626 , 1982
Cells
Microvascular endothelial
Enzymes
Medium
MEM
Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Chicken
Chick embryo
(also rat)
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Heart
Bovine
Bovine
(also rat)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Heart
Chicken
Chicken, embryonic
Blech-Hermoni, Y. and Ladd, A.
Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq. ,
Genom Data
 6 , 74-76 , 2015
Cells
Cardiomyocytes
Enzymes
Medium
HBSS
Heart
Chicken
Chicken embryos, 9-11 day incubated
Eschenhagen, T. , Fink, C. , Remmers, U. , Scholz, H. , Wattchow, J. , Weil, J. and et al.
Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System ,
FASEB J
 11 , 683 , 1997
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.25%
Medium
PBS
Heart
Chicken
Chick, embryos, 10-11 day
Wang, S. , Greaser, M.L. , Schultz, E. , Bulinski, J.C. , Lin, J.J. and Lessard, J.L.
Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin ,
J Cell Biol
 107 , 1075 , 1988
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.17%
Medium
HBSS, CMF
Heart
Chicken
Chick, 10 day
Kim, D. , Okada, A. and Smith, T.W.
Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells ,
Circ Res
 61 , 29 , 1987
Cells
Ventricular
Enzymes
Trypsin : 0.025%
Medium
HBSS, CMF
Heart
Chicken
Chick, embryo, 11 day
Jacob, R. , Lieberman, M. , Murphy, E. and Piwnica-Worms, D.
Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells ,
J Physiol
 387 , 549 , 1987
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.05%
Medium
HBSS, CMF
Heart
Chicken
Chick, embryonic, 11-12-day-old
Murphy, E. , Aiton, J. , Russell, C. and Lieberman, M.
Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury ,
Am J Physiol Cell Physiol
 245 (14) , C316 , 1983
Cells
Myocytes
Enzymes
Trypsin : 0.025%
Medium
CMF solution
Heart
Chicken
Chick, 8 day embryo
(also rat, neonate and mouse)
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Cells
Myocytes
Enzymes
Medium
Rinaldini's buffer solution, CF
Heart
Chicken
Hen, White Leghorn
Dehann, R.
Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture ,
Dev Biol
 16 , 216 , 1967
Cells
Heart
Enzymes
Trypsin : 0.025%
Medium
Medium 199
Heart
Chicken
Chick embryos, 5 day
Steinberg, M.
"ECM": Its Nature, Origin, And Function in Cell Aggregation ,
Exp Cell Res
 30 , 257 , 1963
Cells
Heart
Liver
Enzymes
Trypsin : 3.0%
Medium
Tyrode's solution, CMF
Kidney
Bovine
Bovine
Husted, R. , Hayashi, M. and Stokes, J.
Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
 255 , F1160 , 1988
Cells
Papillary duct
Enzymes
Hyaluronidase : 0.2%
Medium
Keri's buffer
HEPES buffered saline
Liver
Bovine

Bovine, Holstein, 22-23 month

Testroet, E. , Choudhary, S. , Choudhary, R. , Beitz, D. and Du, M.
Tumor Necrosis Factor Alpha and Palmitate Simulate Bovine Fatty Liver Disease in vitro when Using Abattoir-Derived Primary Bovine Hepatocytes Isolated by a Novel Nonperfusion Method. , JDS Commun Vol. 3 , 456-461 , 2022
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
DMEM
Liver
Chicken
Chicken, adult
Fraslin, J. , Touquette, L. , Douaire, M. , Menezo, Y. , Guillemot, J. and Mallard, J.
Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture ,
In Vitro Cell Dev Biol
 28 , 615 , 1992
Cells
Hepatocytes
Enzymes
Collagenase : 0.02%
Medium
HEPES, CF
Liver
Chicken
Chicken
(also rat, SD, 150-250 g)
Weigel, P. , Schnaar, R. , Kuhlenschmidt, M. , Schmell, E. , Lee, R. , Lee, Y. and Roseman, S.
Adhesion of Hepatocytes to Immobilized Sugars ,
J Biol Chem
 254 (21) , 10830 , 1979
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 6000 units
Medium
HEPES
Liver
Chicken
Chicken, Leghorn, white, 10-15 day
Tarlow, D. , Watkins, P. , Reed, R. , Miller, R. , Zwergel, E. and Lane, M.
Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture ,
J Cell Biol
 73 , 332 , 1974
Cells
Hepatocytes
Enzymes
Deoxyribonuclease I : 0.00125%
Medium
PBS
Liver
Chicken
Chick embryos, 5 day
Steinberg, M.
"ECM": Its Nature, Origin, And Function in Cell Aggregation ,
Exp Cell Res
 30 , 257 , 1963
Cells
Heart
Liver
Enzymes
Trypsin : 3.0%
Medium
Tyrode's solution, CMF
Liver
Chicken
Chick embryonic
Rinaldini, L.
An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells ,
Exp Cell Res
 16 , 477 , 1959
Cells
Various tissues (heart, liver, skeletal, cardiac)
Enzymes
Trypsin : various grades
Medium
CMF Tyrode's solution
Lung
Bovine
Bovine
Del Vecchio, P. , Siflinger-Birnboim, A. , Belloni, P. , Holleran, L. , Lum, H. and Malik, A.
Culture and Characterization of Pulmonary Microvascular Endothelial Cells ,
In Vitro Cell Dev Biol
 28A , 711 , 1992
Cells
Endothelial
Pulmonary
Enzymes
Collagenase : 1000 u/ml
Collagenase Type 2 : 1000 u/ml
Medium
PBS, CMF
Mammary
Bovine
Bovine, lactating Holstein
Miranda, S. , Wang, Y. , Purdie, N. , Osborne, V. , Coomber, B. and Cant, J.
Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture. ,
J Dairy Sci
 92 , 2670 , 2009
Cells
Mammary epithelial
Enzymes
Collagenase Type 3 : 400 u/ml
Hyaluronidase : 100 u/ml
Medium
HBSS
Mammary
Bovine
Bovine, heifer, 200kg
Weber M. , Purup S. , Vestergaard M. , Ellis S. , Scndergard-Andersen J. , Akers R. and Sejrsen K.
Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum ,
J Endocrinol
 161 , 365 , 1999
Cells
Mammary epithelial
Enzymes
Medium
M-199
Mammary
Bovine
Bovine
Gibson, C. , Vega, J. , Baumrucker, C. , Oakley, C. and Welsch, C.
Establishment And Characterization Of Bovine Mammary Epithelial Cell Lines ,
In Vitro Cell Dev Biol
 27 , 585 , 1991
Cells
Epithelial
Enzymes
Hyaluronidase : 0.005%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.