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Adipose/Fat
Bovine

Belgian Blue, 1-7 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Bovine
Bovine
Yang, Y. and Baldwin, R.
Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue ,
J Dairy Sci
 56 , 350 , 1973
Cells
Adipocytes
Enzymes
Collagenase Type 1 : 40 u/ml
Medium
Krebs-Ringer bicarbonate
Adipose/Fat
Gerbil
Gerbil of unknown age
(also rat, hamster, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, 5 week-12 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Bovine
Bovine
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Bovine
Bovine, 6 month
Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B.
Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core ,
Cell Transplant
 15 , 121-33 , 2006
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Hahm, S. , Chen, Y. , Vinson, C. and Eiden, L.
A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization. ,
Mol Pharmacol
 64 , 1503 , 2003
Cells
Chromaffin cells
Enzymes
Collagenase : 0.1%
Medium
DMEM
Adrenal
Bovine
Bovine
Ortega, J. , Sagen, J. and Pappas, G.
Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS ,
Cell Transplant
 1 , 33 , 1992
Cells
Chromaffin
Enzymes
Collagenase : 0.2%
Medium
Locke's solution
Adrenal
Bovine
Bovine
Zhu, J. , Li, W. , Toews, M. and Hexum, T.
Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process ,
J Pharmacol Exp Ther
 263 (3) , 1479 , 1992
Cells
Chromaffin
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
(also rat)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Adrenal
Bovine
Bovine
Dahmer, M. , Hart, P. and Perlman, R.
Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells ,
J Neurochem
 54 (3) , 931 , 1990
Cells
Chromaffin
Enzymes
Medium
DMEM
Adrenal
Bovine
Bovine
Higgins, L. and Berg, D.
Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells ,
J Neurosci
 7 (6) , 1792 , 1987
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Aunis, D. , Rotllan, P. and Miras-Portugal, M.
Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture ,
Neurochem Int
 7 , 89 , 1985
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CMF
Adrenal
Bovine
Bovine
Almazan, G. , Aunis, D. , Garcia, A. , Montiel, C. , Nicolas, G. and Sanchez-Garcia, P.
Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells ,
Br J Biomed Sci
 81 , 599 , 1984
Cells
Chromaffin
Enzymes
Collagenase : 0.15%
Medium
Kreb's, CMF
Adrenal
Bovine
Bovine
Pollard, H. , Pazoles, C. , Creutz, C. , Scott, J. , Zinder, O. and Hotchkiss, A.
An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells ,
J Biol Chem
 259 , 1114 , 1984
Cells
Chromaffin
Enzymes
Collagenase : 0.1%
Medium
(see reference)
Adrenal
Bovine
Bovine
Cena, V. , Garcia, A. , Montiel, C. and Sanchez-Garcia, P.
Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells ,
Br J Pharmacol
 81 , 119 , 1984
Cells
Chromaffin
Enzymes
Medium
CF Kreb's
Adrenal
Bovine
Bovine
Knight, D. and Baker, P.
Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells ,
Q J Exp Physiol
 68 , 123 , 1983
Cells
Medulla
Enzymes
Hyaluronidase : 0.2%
Medium
Saline w/BSA 0.5%
Adrenal
Bovine
Bovine
Waymire, J. , Bennett, W. , Boehme, R. , Hankins, L. , Gilmer-Waymire, K. and Haycock, J.
Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture ,
J Neurosci Methods
 7 , 329 , 1983
Cells
Chromaffin
Enzymes
Medium
HBSS, modified
Adrenal
Bovine
Bovine
Greenberg, A. and Zinder, O.
alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells ,
Cell Tissue Res
 226 , 655 , 1982
Cells
Medulla
Enzymes
Collagenase : 0.2%
Medium
Krebs-Ringer bicarbonate buffer, CMF
Adrenal
Bovine
Bovine
Wilson, S.P. and Viveros, O.H.
Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium ,
Exp Cell Res
 133 , 159 , 1981
Cells
Chromaffin
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
Medium A
(see reference)
Adrenal
Bovine
Bovine
Baker, P. and Knight., D
Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells ,
Phil Trans R Soc Lond
 296 , 83 , 1981
Cells
Medulla
Enzymes
Protease : 0.2%
Medium
Saline
Adrenal
Bovine
Bovine
Kilpatrick, D. , Ledbetter, F. , Carson, K. , Kirshner, A. , Slepetis, R. and Kirshner, N.
Stability of Bovine Adrenal Medulla Cells in Culture ,
J Neurochem
 35 (3) , 679 , 1980
Cells
Medulla
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CF
Adrenal
Bovine
Bovine
Aunis, D. , Guerold, B. , Bader, M-F. and Cieselski-Treska, J.
Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells ,
Neuroscience
 5 , 2261 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
DMEM
Adrenal
Bovine
Bovine
Trifaro, J.M. and Lee, R.W.
Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures ,
Neuroscience
 5 , 1533 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CMF
Adrenal
Bovine
Bovine, adult
(also rat, Hanover-Wistar, young; guinea-pig, newborn)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Bovine
Bovine
Kumakura, K. , Karoum, F. , Guidotti, A. and Costa, E.
Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells ,
Nature
 283 , 489 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.25%
Medium
F-12 medium
Adrenal
Bovine
Bovine, adult
Unsicker, K. and Griesser, G.
Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures ,
Neuroscience
 5 , 1445 , 1980
Cells
Medulla
Enzymes
Medium
HBSS
Adrenal
Bovine
Bovine
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Bovine
Bovine
Hersey, R. and DiStefano, V.
Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells ,
J Pharmacol Exp Ther
 209 (1) , 147 , 1979
Cells
Medullary
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
Fenwick, E. , Fajdiga, P. , Howe, N. and Livett, B.
Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell ,
J Cell Biol
 76 , 12 , 1978
Cells
Medulla
Enzymes
Medium
Kreb's, CF
Adrenal
Bovine
Bovine
Brooks, J.C.
The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion ,
Endocrinology
 101 , 1369 , 1977
Cells
Chromaffin
Enzymes
Hyaluronidase : 0.2%
Medium
HEPES, CF
Adrenal
Bovine
Bovine, male
Schneider, A. , Herz, R. and Rosenheck, K.
Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla ,
Proc Natl Acad Sci U S A
 74 , 5036 , 1977
Cells
Chromaffin
Enzymes
Medium
Kreb's Ringer bicarbonate buffer, CF
Adrenal
Hamster
Hamster (Mesocricetus auratus) 100-150 g
Liang, B.T. and Perlman, R.L.
Catecholamine Secretion by Hamster Adrenal Cells ,
J Neurochem
 32 , 927 , 1979
Cells
Adrenal
Chromaffin
Enzymes
Hyaluronidase : 0.20%
Medium
Kreb's Ringer bicarbonate buffer
Bone
Bovine
Bovine
Buschmann, M. , Gluzband, Y. , Grodzinsky, A. and Hunziker, E.
Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture ,
J Cell Sci
 108 , 1497 , 1995
Cells
Chondrocytes
Enzymes
Medium
DMEM
Brain
Bovine
Bovine
Kanda, T. , Yoshino, H. , Ariga, T. , Yamawaki, M. and Yu, R.
Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy ,
J Cell Biol
 126 (1) , 235 , 1994
Cells
Microvascular endothelial
Enzymes
Neutral Protease : 0.005%
Medium
Medium 199

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.