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Tissue References Search Tool

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Muscle
Bovine
Bovine
Lee, E. , Choi, J. , Hyun, J. , Cho, K. , Hwang, I. , Lee, H. , Chang, J. and Choi, I.
Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells. ,
AJAS
 20 , 501 , 2007
Cells
Muscle satellite cells
Enzymes
Collagenase Type 2 : 300 u/ml
Medium
Krebs-Ringer Bicarbonate
Muscle
Bovine
Bovine
Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.
Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. ,
J Appl Physiol
 93 , 1391 , 2002
Cells
Pulmonary artery endothelial and smooth muscle cells
Enzymes
Collagenase : 0.04-0.05%
Medium
RPMI-1640
Muscle
Bovine
Bovine
Absher, M. , Woodcock-Mitchell, J. , Mitchell, J. , Baldor, L. , Low, R. and Warshaw, D.
Characterization of Vascular Smooth Muscle Cell Phenotype in Long-Term Culture ,
In Vitro Cell Dev Biol
 25 (2) , 183 , 1989
Cells
Smooth muscle
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Bovine
Bovine
Warshaw, D. , Szarek, J. , Hubbard, M. and Evans, J.
Pharmacology and Force Development of Single Freshly Isolated Bovine Cartoid Artery Smooth Muscle Cells ,
Circ Res
 58 , 399 , 1986
Cells
Vascular smooth muscle
Enzymes
Elastase Type 3 : 50 u/ml
Medium
PSS
Muscle
Bovine
Bovine
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Muscle
Guinea-Pig
Guinea-pig
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate
Muscle
Guinea-Pig
Guinea pig, 2-4 wk old, male, female
Firth, T. , Mawe, G. and Nelson, M.
Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle ,
Am J Physiol Cell Physiol
 278 , C1031 , 2000
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
Krebs solution
Muscle
Guinea-Pig
Guinea-pig, 200-380 g
Schnitzler, M. , Derst, C. , Daut, J. and Preisig-Muller, R.
ATP-Sensitive Potassium Channels in Capillaries Isolated From Guinea-Pig Heart ,
J Physiol
 525 (2) , 307 , 2000
Cells
Capillaries
Myocytes
Enzymes
Medium
CF solution
Muscle
Guinea-Pig
Guinea-pig, adult, 250-350 g
Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.
Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle ,
Am J Physiol
 277 , G1017 , 1999
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES
Muscle
Guinea-Pig
Guinea-pig, Dunkin-Hartley, female
Ryder, K. , Bryant, S. and Hart, G.
Membrane Current Changes in Left Ventricular Myocytes Isolated From Guinea-Pigs After Abdominal Aortic Coarctation ,
Cardiovasc Res
 27 , 1278 , 1993
Cells
Myocytes
Medium
DMEM
Muscle
Guinea-Pig
Guinea-pig
(also rat, rabbit)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Guinea-Pig
Guinea-pig, 200-300 g
Ohya, Y. and Sperelakis, N.
ATP Regulation of the Slow Calcium Channels in Vascular Smooth Muscle Cells ,
Circ Res
 64 , 145 , 1989
Cells
Smooth muscle
Mesenteric artery
Enzymes
Collagenase : 0.3%
Medium
CF solution
Muscle
Guinea-Pig
Guinea-pig, 200-400 g
(also rabbit, frog, dogfish)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Guinea-Pig
Guinea-pig, prepubertal
Ross, R.
The Smooth Muscle Cell . II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers ,
J Cell Biol
 50 , 172 , 1971
Cells
Smooth muscle
Aortic
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt modification of Eagle's

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.