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Adipose/Fat
Bovine

Belgian Blue, 1-7 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Bovine
Bovine
Yang, Y. and Baldwin, R.
Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue ,
J Dairy Sci
 56 , 350 , 1973
Cells
Adipocytes
Enzymes
Collagenase Type 1 : 40 u/ml
Medium
Krebs-Ringer bicarbonate
Adipose/Fat
Hamster
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, 5 week-12 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Mouse

Mouse, Adult

Hay, A. , Murphy, T. , Popichak, K. , Zabel, M. and Moreno, J.
Adipose-derived mesenchymal stromal cells decrease prion-induced glial inflammation in vitro. ,
Sci Rep
 12 , 22567 , 2022
Cells
Mesenchymal stromal
Enzymes
Stemxyme 1 : 400u/ml
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse
Jones, J. , Rabhi, N. , Orofino, J. , Gamini, R. , Perissi, V. , Vernochet, C. and Farmer, S.
The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. ,
Sci Rep
 10 , 2380 , 2020
Cells
Adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Bowles, A. , Strong, A. , Wise, R. , Thomas, R. , Gerstein, B. , Dutreil, M. , Hunter, R. , Gimble, J. and Bunnell, B.
Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis. ,
Stem Cells
 35 , 532-544 , 2017
Cells
Adipose stromal
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Yao, W. , Lay, Y. , Kot, A. , Liu, R. , Zhang, H. , Chen, H. , Lam, K. and Lane, N.
Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference. ,
Stem Cells
 34 , 2587-2600 , 2016
Cells
Adipose stromal
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse
Maria, O. , Shalaby, M. , Syme, A. , Eliopoulos, N. and Muanza, T.
Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis. ,
Cytotherapy
 18 , 1129-45 , 2016
Cells
Adipose mesenchyal stromal
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Jang, H. , Bhasin, S. , Guarneri, T. , Serra, C. , Schneider, M. , Lee, M. , Guo, W. , Fried, S. , Pencina, K. and Jasuja, R.
The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life. ,
Endocrinology
 156 , 3737 , 2015
Cells
Adipocytes
Enzymes
Collagenase : 0.1%
Medium
Krebs
Adipose/Fat
Mouse
Mouse
Seaman, S. , Tannan, S. , Cao, Y. , Peirce, S. and Lin, K.
Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts. ,
Plast Reconstr Surg
 136 , 189e-199e , 2015
Cells
Adipocytes
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse, 20 week
Kondo, T, Toyoshima, Y. , Ishii, Y. and Kyuwa, S.
Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice. ,
Exp Anim
 62 , 319 , 2013
Cells
Stromal vascular
Enzymes
Medium
RPMI-1640
Adipose/Fat
Mouse
Mouse
Takahashi, H. , Haraguchi, N. , Nishikawa, S. , Miyazaki, S. , Suzuki, Y. , Mizushima, T. , Nishimura, J. , Takemasa, I. , Yamamoto, H. , Mimori, K. , Ishii, H. , Doki, Y. and Mori, M.
Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair. ,
Stem Cells Transl Med
 1 , 803 , 2012
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Han, J. , Koh, Y. , Moon, H. , Ryoo, H. , Cho, C. , Kim, I. and Koh, G.
Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells. ,
Blood
 115 , 957 , 2010
Cells
Stem and progenitor
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Wong, K. , Szeto, F, Zhang, W. , Ye, H. , Kong, J. , Zhang, Z. , Sun, X. and Li, Y
Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins. ,
Am J Physiol/Endo
 296 , 820 , 2009
Cells
White adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Thangarajah, H. , Vial, I. , Chang, E. , El-Ftesi, S. , Januszyk, M. , Chang, E. , Paterno, J. , Neofytou, E. , Longaker, M. and Gurtner, G.
IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia. ,
Stem Cells
 27 , 266 , 2009
Cells
Adipose stromal
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse, 3 week
De Matteis, R. , Zingaretti, M. , Murano, I. , Vitali, A. , Frontini, A. , Giannulis, I. , Barbatelli, G. , Marcucci, F. , Bordicchia, M. , Sarzani, R. , Raviola, E. and Cinti, S.
In Vivo Physiological Transdifferentiation of Adult Adipose Cells. ,
Stem Cells
 27 , 2761 , 2009
Cells
Adipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Cho, K. , Park, H. , Park, H. , Jung, J. , Jeon, S. , Kim, Y. and Roh, H.
IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model. ,
Stem Cells
 27 , 259-65 , 2009
Cells
Adipose tissue-derived stem
Enzymes
Medium
Modified Eagles
Adipose/Fat
Mouse
Mouse, C57Bl/6J
Aoyagi T, Shimba S, Tezuka M.
Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes ,
J Health Sci
 51 , 21 , 2005
Cells
Adipocytes
Enzymes
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse
Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis.
Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives ,
Circulation
 109 , 656-63 , 2004
Cells
Stromal vascular, adipocytes
Enzymes
Collagenase : 0.2%
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse, 3-6 day and 2-3 month
Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T.
Adipose-derived adult stromal cells heal critical-size mouse calvarial defects ,
Nat Biotechnol
 22 , 560-7 , 2004
Cells
Adipose-derived stromal cells
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse, C57BL/6J and FVB, male, 8-9 week
Ruan, H. , Zarnowski, MJ. , Cushman, S. and Lodish, H.
Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes ,
J Biol Chem
 278 , 47585 , 2003
Cells
Adipocytes
Enzymes
Collagenase : see reference
Medium
DMEM
Adipose/Fat
Mouse
Mouse, both sexes
Nadler, S. , Stoehr, J. , Rabaglia, M. , Schueler, K. , Birnbaum, M. and Attie, A.
Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice ,
Am J Physiol/Endo
 281 , E1249 , 2001
Cells
Adipocytes
Enzymes
Collagenase : 0.05%
Medium
Krebs-Ringer Phosphate HEPES (KRPH)
Adipose/Fat
Mouse
Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male
Launder, T. , Gegen, N. , Knedler, A. and Harbeck, R.
The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue ,
J Immunol Methods
 102 , 45 , 1987
Cells
Vascular endothelial
Enzymes
Collagenase : 0.2%
Medium
PBS
Adrenal
Bovine
Bovine
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Bovine
Bovine, 6 month
Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B.
Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core ,
Cell Transplant
 15 , 121-33 , 2006
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Hahm, S. , Chen, Y. , Vinson, C. and Eiden, L.
A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization. ,
Mol Pharmacol
 64 , 1503 , 2003
Cells
Chromaffin cells
Enzymes
Collagenase : 0.1%
Medium
DMEM
Adrenal
Bovine
Bovine
Ortega, J. , Sagen, J. and Pappas, G.
Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS ,
Cell Transplant
 1 , 33 , 1992
Cells
Chromaffin
Enzymes
Collagenase : 0.2%
Medium
Locke's solution
Adrenal
Bovine
Bovine
Zhu, J. , Li, W. , Toews, M. and Hexum, T.
Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process ,
J Pharmacol Exp Ther
 263 (3) , 1479 , 1992
Cells
Chromaffin
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
(also rat)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Adrenal
Bovine
Bovine
Dahmer, M. , Hart, P. and Perlman, R.
Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells ,
J Neurochem
 54 (3) , 931 , 1990
Cells
Chromaffin
Enzymes
Medium
DMEM
Adrenal
Bovine
Bovine
Higgins, L. and Berg, D.
Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells ,
J Neurosci
 7 (6) , 1792 , 1987
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Aunis, D. , Rotllan, P. and Miras-Portugal, M.
Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture ,
Neurochem Int
 7 , 89 , 1985
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CMF
Adrenal
Bovine
Bovine
Almazan, G. , Aunis, D. , Garcia, A. , Montiel, C. , Nicolas, G. and Sanchez-Garcia, P.
Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells ,
Br J Biomed Sci
 81 , 599 , 1984
Cells
Chromaffin
Enzymes
Collagenase : 0.15%
Medium
Kreb's, CMF
Adrenal
Bovine
Bovine
Pollard, H. , Pazoles, C. , Creutz, C. , Scott, J. , Zinder, O. and Hotchkiss, A.
An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells ,
J Biol Chem
 259 , 1114 , 1984
Cells
Chromaffin
Enzymes
Collagenase : 0.1%
Medium
(see reference)
Adrenal
Bovine
Bovine
Cena, V. , Garcia, A. , Montiel, C. and Sanchez-Garcia, P.
Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells ,
Br J Pharmacol
 81 , 119 , 1984
Cells
Chromaffin
Enzymes
Medium
CF Kreb's

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.