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Tissue References Search Tool

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Endothelial
Bovine
Bovine
DeNucci, G. , Gryglewski, R.J. , Warner, T.D. and Vane, J.R.
Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled ,
Proc Natl Acad Sci U S A
 85 , 2334 , 1988
Cells
Endothelial
Aortic
Enzymes
Trypsin : 0.05%
Medium
Krebs Ringer solution
Endothelial
Bovine
Bovine
Gospodarowicz, D. , Massoglia, S. , Cheng, J. and Fujii, D.
Effect of Fibroblast Growth Factor and Lipoproteins on the Proliferation of Endothelial Cells Derived From Bovine Adrenal Cortex, Brain Cortex, and Corpus Luteum Capillaries ,
J Cell Physiol
 127 , 121 , 1986
Cells
Endothelial
Enzymes
Collagenase : 0.5%
Medium
DMEM/Ham's F-12
Endothelial
Bovine
Bovine
Carson, M.P. and Haudenschild, C.C.
Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures ,
In Vitro Cell Dev Biol
 22 , 344 , 1986
Cells
Endothelial
Aorta
Enzymes
Medium
PBS
Endothelial
Bovine
Bovine
Kinsella, Michael G. and Wight, Thomas N.
Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration ,
J Cell Biol
 102 , 679 , 1986
Cells
Endothelial
Aortic
Enzymes
Medium
DMEM
Endothelial
Bovine
Bovine
Goetz, I.. , Warren, J. , Estrada, C. , Roberts, E. and Krause, D.
Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain ,
In Vitro Cell Dev Biol
 21 , 172 , 1985
Cells
Endothelial
Brain arteries
Enzymes
Medium
Dulbecco's PBS
Endothelial
Bovine
Calf
Voyta, J. , Via, D. , Butterfield, C. and Zetter, B.
Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein ,
J Cell Biol
 99 , 2034 , 1984
Cells
Endothelial
Smooth muscle
Enzymes
Collagenase : 0.75%
Medium
DMEM
Endothelial
Bovine
Bovine
Scott, D. , Murray, J. and Barnes, M.
Investigation of the Attachment of Bovine Corneal Endothelial Cells ,
Exp Cell Res
 144 , 472 , 1983
Cells
Endothelial
Corneal
Enzymes
Trypsin : 0.2%
Medium
PBS: DMEM
Endothelial
Bovine
Bovine
Robinson, J. and Gospodarowicz, D.
Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells ,
J Cell Physiol
 117 , 368 , 1983
Cells
Endothelial, Corneal
Enzymes
Trypsin : 0.05%
Medium
0.01M Phosphate buffer with 0.02% EDTA
0.9% NaCl (See Reference)
Endothelial
Bovine
Bovine, 2-3 weeks
Lee, S. , Douglas, W. , Deneke, S. and Fanburg, B.
Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro ,
In Vitro
 19 , 714 , 1983
Cells
Endothelial
Pulmonary artery
Enzymes
Medium
RPMI 1640 w/1% Fetal Bovine Serum
Endothelial
Bovine
Bovine
Olander, J. , Marasa, J. , Kimes, R. , Johnston, G. and Feder, J.
An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells ,
In Vitro
 18 , 99 , 1982
Cells
Endothelial
Subclavian vein
Enzymes
Medium
PBS
Endothelial
Bovine
Bovine
Makarski, J. S.
Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells ,
In Vitro
 17 , 450 , 1981
Cells
Aortic
Pulmonary artery
Enzymes
Medium
PBS
Endothelial
Bovine
Calf, fetal, 4-9 months
Rosen, E. , Mueller, S. , Noveral, J. and Levine, E.
Proliferative Characteristics of Clonal Endothelial Cell Strains ,
J Cell Physiol
 107 , 123 , 1981
Cells
Endothelial
Enzymes
Medium
PBS
Endothelial
Bovine
Bovine, young
Ryan, U. , Mortara, M. and Whitaker, C.
Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes ,
Tissue Cell
 12 , 619 , 1980
Cells
Pulmonary artery
Enzymes
Collagenase : 0.1%
Medium
Medium 199
Endothelial
Bovine
Bovine, adult
Cotta-Pereira, G. , Sage, H. , Bornstein, P. , Ross, R. and Schwartz, S.
Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis ,
J Cell Physiol
 102 , 183 , 1980
Cells
Aorta
Enzymes
Collagenase Type 1 : 125 u/ml
Medium
Dulbecco's PBS with calcium and magnesium
Endothelial
Bovine
Bovine
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Bovine
Bovine
Eskin, S. , Sybers, H. , Trevino, L. , Lie, J. and Chimoskey, J.
Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein ,
In Vitro
 14 , 903 , 1978
Cells
Saphenous Vein
Aorta
Enzymes
Collagenase : 0.01%
Medium
PBS
Endothelial
Bovine
Bovine
Ryan, U.S. , Clements, E. , Habliston, D. and Ryan, J.W.
Isolation And Culture of Pulmonary Artery Endothelial Cells ,
Tissue Cell
 10 , 535 , 1978
Cells
Pulmonary artery
Enzymes
Medium
Puck's solution
Endothelial
Bovine
Bovine
Schwartz, S.M.
Selection and Characterization of Bovine Aortic Endothelial Cells ,
In Vitro
 14 , 966 , 1978
Cells
Endothelial
Thoracic aorta
Saphenous veins
Enzymes
Medium
PBS
Endothelial
Bovine
Calf
Howard, B. , Macarak, E. , Gunson, D. and Kefalides, N.
Characterization of the Collagen Synthesized by Endothelial Cells in Culture ,
Proc Natl Acad Sci U S A
 73 (7) , 2361 , 1976
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Rat
Rat, SD, male, 250-300 g
Schwertschlag, U.S. and Whorton, A.R.
Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
 263 , 13791 , 1988
Cells
Smooth muscle, aorta
Enzymes
Medium
HBSS with 0.2 mM Ca++
Endothelial
Rat
Rat, SD, male, 350 - 450 g
Friedman, S. and Roll, F.
Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan ,
Anal Biochem
 161 , 207 , 1987
Cells
Lipocytes
Kupffer
Sinusoidal endothelial
Enzymes
Collagenase : 0.015%
Medium
DMEM/Ham's F-12
Endothelial
Rat
Rat, Wistar, male, 3 mo
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Endothelial
Rat
Rat, Wistar-Kyoto, male, 100 - 200 g
Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J.
Primary Culture of Rat Cerebral Microvascular Endothelial Cells ,
Lab Invest
 46 , 554 , 1982
Cells
Endothelial
Cerebral
Enzymes
Medium
HBSS
Endothelial
Rat
Rat, 300 G, and pig, 30-40 Kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Rat
Rat
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS
Epithelial
Bovine
Bovine, fetal
Schumann, B.L. , Cody, T.E. , Miller, M.L. and Leikauf, G.D.
Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells ,
In Vitro Cell Dev Biol
 24 , 211 , 1988
Cells
Epithelial
Tracheal
Enzymes
Medium
Dissociation medium, CMF
Epithelial
Rat
Rat
Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.
The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
 115 , 412 , 2010
Cells
Mammary epithelial
Enzymes
Medium
HBSS
Epithelial
Rat
Rat, SD, adult, male, 8-10 weeks old
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Epithelial
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, fetal, 14-15 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Epithelial
Rat
Rat, 6 day old
Evans GS, Flint N, Somers AS, Eyden B. and Potten CS.
The development of a method for the preparation of rat intestinal epithelial cell primary cultures ,
J Cell Sci
 101 , 219 , 1992
Cells
Rat intestinal epithelial
Enzymes
Collagenase : 300 u/ml
Medium
DMEM
Epithelial
Rat
Rat, SD, adult, 90-120 day old, 350-450 g
Klinefelter, G.
A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats ,
J Tiss Cul Meth
 14 , 195 , 1992
Cells
Epididymal epithelial
Enzymes
Medium
HBSS
Epithelial
Rat
Rat, adult
(also hamster)
Heimann, T. and Githens, S.
Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix ,
Pancreas
 6 (5) , 514 , 1991
Cells
Interlobular duct fragments
Medium
DMEM/Ham's F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.