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Tissue References Search Tool

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Kidney
Mouse
Mouse, male, 2 month
Sindic, A. , Velic, A. , Basoglu, C. , Hirsch, J. , Edemir, B. , Kuhn, M. and Schlatter, E.
Uroguanylin and Guanylin Regulate Transport of Mouse Cortical Collecting Duct Independent of Guanylate Cyclase C. ,
Kidney Int
 68 , 1008 , 2005
Cells
Cortical collecting duct
Enzymes
Collagenase : 54-178 u/ml
Protease : see reference
Medium
MEM
Kidney
Mouse
Mouse, male, 12-16 week
Cunningham, R. , Xiaofei, E. , Steplock, D. , Shenolikar, S. and Weinman, E.
Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media. ,
Am J Physiol Renal Physiol
 289 , F933 , 2005
Cells
Renal proximal tubule cells
Enzymes
Medium
DMEM/F-12
Kidney
Mouse
Mouse, 6 week
Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE.
Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis ,
J Biol Chem
 277(30) , 27535 , 2002
Cells
Glomerular mesangial cells
Enzymes
Medium
RPMI 1640
Kidney
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Kidney
Mouse
Mouse
Taub, M.
Renal Tubule Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley & Sons, Ltd. , 14B:6.1 , 1995
Cells
Epithelial
Enzymes
Medium
DMEM /F-12
Kidney
Mouse
Mouse, naive SJL (H-2S)
Haverty, T. , Kelly, C. , Hines, W. , Amenta, P. , Watanabe, M. , Harper, R. , Kefalides, N. and Neilson, E.
Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis ,
J Cell Biol
 107 , 1359 , 1988
Cells
Proximal tubular epithelial
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
RPMI 1640
Liver
Canine
Dog, 13 years old
(also rat, guinea-pig, rabbit, human)
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Canine
Dog, adult
Vickrey, H. , Ramon, J. and McCann, D.
Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions ,
In Vitro
 15 , 120 , 1979
Cells
Hepatocytes
Enzymes
Trypsin : 0.1%
Medium
HBSS, CMF
Liver
Mouse

Mouse

Bahrami, E. , Geiger, T. , Steixner-Kumar, A. , Santacruz, D. , Viollet, C. and Dick, A.
An Optimized Protocol for Isolation of Hepatic Leukocytes Retrieved from Murine and NASH Liver Biopsies. ,
Star Protocols
 4 , 102597 , 2023
Cells
Leukocytes
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
RPMI-1640
Liver
Mouse

7-10 week

Plata-Gómez, A. , Crespo, M. , Arregui, C. , Prado-Rivas, L. , Sabio, G. and Efeyan, A.
Protocol for the Assessment of mTOR Activity in Mouse Primary Hepatocytes. , STAR Protoc Vol. 2 , 100918 , 2021
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Mouse

Mouse

Jung, Y. , Zhao, M. and Svensson, K.
Isolation, Culture, and Functional Analysis of Hepatocytes from Mice with Fatty Liver Disease. ,
Star Protocols
 1 , 100222 , 2020
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Mouse
Mouse
Cabral, F. , Miller, C. , Kudrna, K. , Hass, B. , Daubendiek, J. , Kellar, B. and Harris, E.
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. ,
J Vis Exp
 , 2018
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Mouse
Mouse
Bigorgne, A. , John, B. , Ebrahimkhani, M. , Shimizu-Albergine, M. , Campbell, J. and Crispe, I.
TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota. ,
PLoS ONE
 11 , e0151063 , 2016
Cells
Hepatic
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, 10-13 week
Sewing, S. , Boess, F. , Moisan, A. , Bertinetti-Lapatki, C. , Minz, T. , Hedtjaern, M. , Tessier, Y. , Schuler, F. , Singer, T. and Roth, A.
Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs. ,
PLoS ONE
 11 , e0159431 , 2016
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 0.2 u/ml
Medium
William's Medium E
Liver
Mouse
Mouse
Weerasinghe, S. , Park, M. , Portney, D. and Omary, M.
Mouse Genetic Background Contributes to Hepatocyte Susceptibility to Fas-mediated Apoptosis. ,
Mol Biol Cell
 27 , 3005-3012 , 2016
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
PBS
Liver
Mouse
Mouse, 12 week
Mederacke, I. , Dapito, D. , Affo, S. , Uchinami, H. and Schwabe, R.
High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers. ,
Nat Protoc
 10 , 305-15 , 2015
Cells
Hepatic stellate
Enzymes
Pronase : 0.04%
Medium
DMEM
Liver
Mouse
Mouse
Guenthner, C. , Luitje, M. , Pyle, L. , Molyneux, P. , Yu, J. , Li, A. , Leise, T. and Harrington, M.
Circadian Rhythms of Per2::Luc in Individual Primary Mouse Hepatocytes and Cultures. ,
PLoS ONE
 9 , e87573 , 2014
Cells
Hepatocytes
Enzymes
Collagenase Type 3 : 100 u/ml
Collagenase Type 4 : 100 u/ml
Medium
DMEM
Liver
Mouse
Mouse
Sin, Y. , Ballantyne, L. , Mukherjee, K. , St Amand, T. , Kyriakopoulou, L. , Schulze, A. and Funk, C.
Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism. ,
PLoS ONE
 8 , e80001 , 2013
Cells
hepatocytes
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
DMEM
Liver
Mouse
Mouse
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, female, 8-10 week
Severgnini, M. , Sherman, J. , Sehgal, A. , Jayaprakash, N. , Aubin, J. , Wang, G. , Zhang, L. , Peng, C. , Yucius, K. , Butler, J. and Fitzgerald, K.
A Rapid Two-Step Method for Isolation of Functional Primary Mouse Hepatocytes: Cell Characterization and Asialoglycoprotein Receptor Based Assay Development. ,
Cytotechnology
 64 , 187-95 , 2012
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Mouse
Mouse
Brundert, M. , Heeren, J. , Merkel, M. , Carambia, A. , Herkel, J. , Groitl, P. , Dobner, T. , Ramakrishnan, R. , Moore, K. and Rinninger, F.
Scavenger Receptor CD36 Mediates Uptake of High Density Lipoproteins in Mice and by Cultured Cells. ,
J Lipid Res
 52 , 745 , 2011
Cells
Hepatocytes & non-parenchymal liver cells
Enzymes
Collagenase : 0.03-0.05%
Medium
DMEM
Liver
Mouse
Mouse
Kang, H. , Okamoto, K. , Kim, Y. , Takeda, Y. , Bortner, C. , Dang, H. , Wada, T. , Xie, W. , Yang, X. , Liao, G. and Jetten, A.
Nuclear Orphan Receptor TAK1/TR4-Deficient Mice are Protected Against Obesity-Linked Inflammation, Hepatic Steatosis, and Insulin Resistance. ,
Diabetes
 60 , 177 , 2011
Cells
Hepatocytes
Enzymes
HIS kit : with modifications
Medium
DMEM
Liver
Mouse
Mouse
Holl, D. , Kuckenberg, P. , Woynecki, T. , Egert, A. , Becker, A. , Huss, S. , Stabenow, D. , Zimmer, A. , Knolle, P. , Tolba, R. , Fischer, H. and Schorle, H.
Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia. ,
PLoS ONE
 6 , e22034 , 2011
Cells
Hepatacytes
Enzymes
Medium
Williams E
Liver
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Liver
Mouse
Mouse
Shmarakov, I. , Fleshman, M. , D'Ambrosio, D. , Piantedosi, R. , Riedl, K. , Schwartz, S. , Curley, R. , von Lintig, J. , Rubin, L. , Harrison, E. and Blaner, W.
Hepatic Stellate Cells are an Important Cellular Site for β-Carotene Conversion to Retinoid. ,
Arch Biochem Biophys
 504 , 3-10 , 2010
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, male, 12-14 week
Chung, S. , Timmins, J. , Duong, M. , Degirolamo, C. , Rong, S. , Sawyer, J. , Singaraja, R. , Hayden, M. , Maeda, N. , Rudel, L. , Shelness, G. and Parks, J.
Targeted Deletion of Hepatocyte ABCA1 Leads to VLDL Triglyceride Over-Production and LDL Hypercatabolism ,
J Biol Chem
 285 , 12197 , 2010
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Mouse
Mouse
Bosschaerts, T. , Guilliams, M. , Stijlemans, B. , Morias, Y. , Engel, D. , Tacke, F. , Herin, M. , De Baetselier, P. and Beschin, A.
Tip-DC Development During Parasitic Infection is Regulated by IL-10 and Requires CCL2/CCR2, IFN-gamma and MyD88 Signaling. ,
PLoS Pathog
 6 , e1001045 , 2010
Cells
Liver non-parenchymal
Enzymes
Collagenase Type 3 : 100 u/ml
Medium
HBSS
Liver
Mouse
Mouse, male, adult
Mathijs, K. , Kienhuis, A. , Brauers, K. J, Jennen, D. , Lahoz, A. , Kleinjans, J. and van Delft, J.
Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes. ,
Drug Metab Dispos
 37 , 1305 , 2009
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, 7-10 week
Follenzi, A. , Benten, D. , Novikoff, P. , Faulkner, L. , Raut, S. and Gupta, S.
Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. ,
J Clin Invest
 118 , 935 , 2008
Cells
Liver endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Liver
Mouse
Mouse, 10-12 week
Beldi, G. , Wu, Y. , Sun, X. , Imai, M. , Enjyoji, K. , Csizmadia, E. , Candinas, D. , Erb, L. and Robson, S.
Regulated Catalysis of Extracellular Nucleotides by Vascular CD39/ENTPD1 is Required for Liver Regeneration. ,
Gastroenterol
 135 , 1751 , 2008
Cells
Liver sinusoidal endothelial cells
Enzymes
Medium
HEPES
Liver
Mouse
Mouse, male
Oliva, J. , Bardag-Gorce, F. , French, B. , Li, J. , McPhaul, L. , Amidi, F. , Dedes, J. , Habibi, A. , Nguyen, S. and French, S.
Fat10 is an Epigenetic Marker for Liver Preneoplasia in a Drug-Primed Mouse Model of Tumorigenesis. ,
Exp Mol Pathol
 84 , 102 , 2008
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Elastase : 0.1 u/ml
Medium
Williams E
Liver
Mouse
Mouse
Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping.
Isolation and characterization of bipotent liver progenitor cells from adult mouse ,
Stem Cells
 24 , 322-32 , 2006
Cells
Liver epithelial progenitor cells
Enzymes
Medium
DMEM
Liver
Mouse
Mouse
Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev.
Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice ,
Hepatol
 42 , 140-8 , 2005
Cells
Liver sinusodial endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Liver
Mouse
Mouse
Jiang Guoqiang, Li Zhihua, Liu Franklin, Ellsworth Kenneth, Dallas-Yang Qing, Wu Margaret, Ronan John, Esau Christine, Murphy Cain, Szalkowski Deborah, Bergeron Raynald, Doebber Thomas, Zhang Bei.
Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1 ,
J Clin Invest
 115 , 1030-8 , 2005
Cells
Hepatocytes
Enzymes
Medium
Leffert's buffer
Liver
Mouse
Mouse, 8-10 week
Kotton, D. , Fabian, A. and Mulligan, R.
A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity. ,
Blood
 106 , 1574 , 2005
Cells
Liver derived stem cells
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, male, 8 week
Hatano E, Brenner DA.
Akt protects mouse hepatocytes from TNF-alpha- and Fas-mediated apoptosis through NK-kappa B activation. ,
Am J Physiol/Gastro
 282 , G1357 , 2002
Cells
Hepatocytes
Enzymes
Medium
Waymouth's medium

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.