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Tissue References Search Tool

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Neural
Chicken
Chick, White Leghorn, embryos
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Neural
Chicken
Chicken, White Leghorn, embryos, 17-21 day
Raman, I. and Trussell, L.
The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus ,
Neuron
 9 , 173 , 1992
Cells
Neurons
Enzymes
Papain : 40 u/ml
Medium
HEPES
Neural
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Neural
Chicken
Chick, White Leghorn, embryo
(also rat, SD, embryo)
Schnaar, R. and Schaffner, A.
Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions ,
J Neurosci
 1 , 204 , 1981
Cells
Spinal cord
Enzymes
Trypsin : 0.05%
Medium
Phosphate buffer
(see reference)
Neural
Chicken
Chick
Choi, D. and Fiscbach, G.
GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture ,
J Neurophysiol
 45 , 605 , 1981
Cells
Dorsal root ganglion neurons
Spinal cord
Enzymes
Trypsin : 0.1%
Medium
Puck's saline, CMF
Neural
Chicken
Chick, White Leghorn embryo, 8 day
Bottenstein, J. , Skaper, S. , Varon, S. and Sato, G.
Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium ,
Exp Cell Res
 125 , 183 , 1980
Cells
Neurons, ganglia
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Chicken
Chicken, White Leghorn, fertile eggs
Tuttle, J. , Suszkiw, J. and Ard, M.
Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture ,
Brain Res
 183 , 161 , 1980
Cells
Ciliary ganglion neurons
Enzymes
Trypsin : 0.25%
Medium
Eagle's MEM
Neural
Chicken
Chicken, embryos, 9-10 days old
Mudge, A. , Leeman, S. and Fischbach, G.
Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration ,
Proc Natl Acad Sci U S A
 76 (1) , 526 , 1979
Cells
Dorsal root ganglia neurons
Enzymes
Collagenase : 0.01%
Medium
Eagle's MEM
Neural
Chicken
Chick embryos
McCarthy, K. and Partlow, L.
Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates ,
Brain Res
 114 , 391 , 1976
Cells
Ganglion chains, sympathetic ganglia
Enzymes
Trypsin : 0.25%
Medium
Krebs Phosphosaline
Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Neural
Fish
Zebrafish, embryonic
Spiro, Z. , Koh, A. , Tay, S. , See, K. and Winkler, C.
Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish. ,
Sci Rep
 6 , 27470 , 2016
Cells
Motorneurons
Enzymes
PDS kit : per instructions
Medium
L-15
Neural
Fish
Zebrafish
Sakowski, S. , Lunn, J. , Busta, A. , Palmer, M. , Dowling, J. and Feldman, E.
A Novel Approach to Study Motor Neurons from Zebrafish Embryos and Larvae in Culture. ,
J Neurosci Methods
 205 , 277-82 , 2012
Cells
Motor neurons
Enzymes
Medium
See reference
Neural
Fish
Zebrafish, Dania rerio, embryo
Won, Y. , Ono, F. and Ikeda, S.
Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons. ,
J Neurophysiol
 105 , 442-53 , 2011
Cells
Rohon-Beard neurons
Enzymes
Trypsin : 0.2%
Medium
L-15/Hepes
Neural
Fish
Zebrafish, Danio rerio, embryo
Cerda, G. , Hargrave, M. and Lewis, K.
RNA Profiling of FAC-sorted Neurons from the Developing Zebrafish Spinal Cord. ,
Dev Dyn
 238 , 150-61 , 2009
Cells
Neurons
Enzymes
PDS kit : with modifications
Medium
EBSS
Neural
Fish
Black ghose knige fish, adult (Apteronotus albifrons)
Anderson, M.J.
Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro ,
In Vitro Cell Dev Biol
 29A , 145 , 1993
Cells
Neurons, spinal cord
Enzymes
Trypsin : 0.4%
Medium
PBS, CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.