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Tissue References Search Tool

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Adipose/Fat
Guinea-Pig
Guinea-pig, adult
(also rat, hamster, gerbil, rabbit, lamb)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Porcine

Porcine, 2-6 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Porcine
Porcine, female, <1 year
Williams, K. , Picou, A. , Kish, S. , Giraldo, A. , Godke, R. and Bondioli, K
Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells. ,
Cells Tissues Organs
 188 , 251 , 2008
Cells
Adipose mesenchymal stem
Enzymes
Medium
DMEM
Adipose/Fat
Porcine
Porcine, crossbred, male 1-4 day
Ramsay, T.G.
Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes ,
J Anim Sci
 81 , 3008 , 2003
Cells
Adipocytes
Enzymes
Medium
DMEM/F12
Adipose/Fat
Porcine
Porcine, crossbred
Liang, W. and Mills, S.
Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues. ,
J Anim Sci
 80 , 963-70 , 2002
Cells
Adipocytes
Enzymes
Medium
Krebs-Ringer
Adipose/Fat
Porcine
Porcine, neonatal
Wang Y, Fried SK, Petersen RN, Schoknecht PA.
Somatotropin regulates adipose tissue metabolism in neonatal swine ,
J Nutr
 129 , 139-45 , 1999
Cells
Adipocytes
Enzymes
Medium
Krebs-Ringer bicarbonate albumin
Adipose/Fat
Porcine
Porcine, 8-9 week
Ding, S. , McNeel, R. and Mersmann, H.
Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo ,
Comp Biochem Physiol B
 123 , 307 , 1999
Cells
Adipose
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
HEPES
Adipose/Fat
Porcine
Porcine, crossbred, 1-3 day
Suryawan, A. , Swanson, L. and Hu, C.
Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture ,
J Anim Sci
 75 , 105 , 1997
Cells
Adipose, Stromal-vascular
Enzymes
Medium
DMEM/F12
Adrenal
Guinea-Pig
Guinea-pig, 500-700 g
Role, L.W. , Leeman, S.E. and Perlman, R.L.
Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla ,
Neurochem Int
 6 , 1813 , 1981
Cells
Chromaffin
Medulla
Medium
BSS
(see reference)
Adrenal
Guinea-Pig
Guinea-pig
(also rat, Hanover-Wistar, young; newborn; cattle)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Guinea-Pig
Guinea-pig
Hochman, J. and Perlman, R.L.
Catecholamine Secretion by Isolated Adrenal Cells ,
Biochim Biophys Acta
 421 , 168 , 1976
Cells
Adrenal
Chromaffin
Enzymes
Collagenase : 0.05%-0.20%
Medium
Kreb's-Ringer bicarb glucose buffer, CF
Bone
Chicken
Chick, day old
Gay, C. , Lloyd, Q. and Gilman, V.
Characteristics and Culture of Osteoblasts Derived From Avian Long Bone ,
In Vitro Cell Dev Biol
 30A , 379 , 1994
Cells
Osteoblasts
Enzymes
Trypsin : 0.03%
Medium
DMEM
Bone
Chicken
Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus)
Rosselot, G. , Reginato, A.M. and Leach, R.M.
Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes ,
In Vitro Cell Dev Biol
 28A , 235 , 1992
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F12
Bone
Chicken
Chick, embryo
Schiltz, J.R. , Mayne R. and Holtzer, H.
The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture ,
Differentiation
 1 , 97 , 1973
Cells
Vertebrae chondroblasts
Enzymes
Trypsin : 0.25%
Medium
Simm's, CMF
Brain
Guinea-Pig
Guinea-pig, 200-400 g
Kay, A.R. and Wong, R.K.S.
Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems ,
J Neurosci Methods
 16 , 227 , 1986
Cells
Neurons
Enzymes
Trypsin : 0.06-0.08%
Medium
PIPES saline
Brain
Porcine
Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months
Robinson, D.H. , Kang,Y. , Deschner, S.H. and Nielsen, T.B.
Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture ,
In Vitro Cell Dev Biol
 26 , 169 , 1990
Cells
Microvascular
Enzymes
Collagenase : 0.1%
Medium
HBSS
Cartilage
Chicken
Chick embryos, HH stage
Wong, M. and Tuan, R.
Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture ,
In Vitro Cell Dev Biol
 29A , 917 , 1993
Cells
Mesenchymal
Enzymes
Trypsin : 0.1%
Medium
DMEM
(see reference)
Cartilage
Chicken
Chicken, broiler strain, 8-10 weeks
Genge, B. , Wu, L. and Wuthier, R.
Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins ,
J Biol Chem
 265 , 4703 , 1990
Cells
Matrix vesicles
Epiphyseal growth plate
Enzymes
Trypsin : 0.1%
Medium
Tris-buffered saline
Cartilage
Chicken
Chicken, broiler strain, 8-10 week
Genge, B.R. , Wu, L.N.Y. and Wuthier, R.E.
Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles ,
J Biol Chem
 264 , 10917 , 1989
Cells
Matrix vesicles
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Cartilage
Chicken
Chick embryos, White Leghorn, 19 day old
Gionti, E. , Capasso, O. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro ,
J Biol Chem
 258 (11) , 7190 , 1983
Cells
Fibroblasts
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
E 199 medium
Cartilage
Chicken
Chick embryos, 19 day old
Capasso, O. , Gionti, E. , Pontarelli, G. , Ambesi-Impiobato, F. , Nitsch, L. , Tajana, G. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro ,
Exp Cell Res
 142 , 197 , 1982
Cells
Chondrocytes
Enzymes
Trypsin : 0.75%
Medium
Coon's modified F-12
Cartilage
Chicken
Chick embryos, White Leghorn
Ahrens, P. , Solursh, M. and Reiter, R.
Stage-Related Capacity for Limb Chondrogenesis in Cell Culture ,
Dev Biol
 60 , 69 , 1977
Cells
Wing buds
Enzymes
Trypsin : 0.1%
Medium
Saline G
Cartilage
Porcine
Porcine, 1 year
Chowdhury, T. , Schulz, R. , Rai, S. , Thuemmler, C. , Wuestneck, N. , Bader, A. and Homandberg, G
Biomechanical Modulation of Collagen Fragment-Induced Anabolic and Catabolic Activities in Chondrocyte/Agarose Constructs. ,
Arthritis Res Ther
 12 , R82 , 2010
Cells
Chondrocytes
Enzymes
Medium
DEMEM
Cartilage
Porcine
Porcine, 2-4 month
Graff RD, Lazarowski ER, Banes AJ, Lee GM.
ATP release by mechanically loaded porcine chondrons in pellet culture ,
Arthritis Rheum
 43 , 1571-9 , 2000
Cells
Chondrons
Enzymes
Medium
PBS
Colon
Guinea-Pig
Guinea-pig, adult
Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D.
Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. ,
J Physiol
 591 , 5959-75 , 2013
Cells
Enterochromaffin
Enzymes
Trypsin : 0.05%
Medium
DMEM
Colon
Guinea-Pig
Guinea-Pig, male, 200-250g
Kang, M. , Maguma, H. , Smith, T. , Ross, G. , Dewey, W. and Akbarali, H.
The Role of β-arrestin2 in the Mechanism of Morphine Tolerance in the Mouse and Guinea Pig Gastrointestinal Tract. ,
J Pharmacol Exp Ther
 340 , 567-76 , 2012
Cells
Myenteric ganglia
Enzymes
Protease : 0.1%
Medium
Kreb's solution
Endothelial
Guinea-Pig
Guinea pig, female, 300-350g
Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J.
Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium ,
Am J Physiol Heart Circ Physiol
 281 , H1657-66 , 2001
Cells
Coronary endothelial
Enzymes
Medium
see reference
Endothelial
Porcine
Porcine, 6 month
Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
 10 , e0130749 , 2015
Cells
Valvular endothelial
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine
Gould, R. and Butcher, J.
Isolation of Valvular Endothelial Cells. ,
J Vis Exp
 46 , e2158 , 2010
Cells
Valvular endothelial
Enzymes
Collagenase Type 2 : 300-600 u/ml
Medium
DMEM
Endothelial
Porcine
Porcine
(also bovine)
Nugent, H. and Edelman, E.
Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury ,
J Surg Res
 99 , 228 , 2001
Cells
Endothelial
Medium
DMEM w/ 10% calf serum
Endothelial
Porcine
Porcine
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY. and Koh GY.
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells ,
Circulation
 101(19) , 2317 , 2000
Cells
HUVEC, porcine pulmonary arterial endothelial cells
Enzymes
Medium
DMEM
Endothelial
Porcine
Porcine, 6-7 month
Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM. and Tisher CC.
Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV ,
J Am Soc Nephrol
 10(3) , 481 , 1999
Cells
Porcine pulmonary endothelial
Enzymes
Medium
RPMI 1640
Endothelial
Porcine
Porcine
Shasby, S.
Endothelial Cells Grown On Permeable Membrane Supports ,
J Tiss Cul Meth
 14 , 247 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
Medium 199
Endothelial
Porcine
Porcine
Vischer, P. and Buddecke, E.
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells ,
Exp Cell Res
 158 , 15 , 1985
Cells
Endothelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Endothelial
Porcine
Porcine
Dickinson, E. and Slakey, L.
Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta ,
In Vitro
 18 , 63 , 1982
Cells
Endothelial
Aortic
Enzymes
Medium
Dulbecco-Vogt MEM w/o serum
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.