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Liver
Rat
Rat, SD, male, 200 g
Rubin, K. , Kjellen, L. and Oslashbrink, B.
Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel ,
Exp Cell Res
 109 , 413 , 1977
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
Buffers 1 & 2
(see reference)
Liver
Rat
Rat (WAG/RIJ), female, 24, 30, and 37 months
VanBezodijen, C. , Grell, T. and Knook, D.
Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells ,
Mech Ageing Dev
 6 , 293 , 1977
Cells
Parenchymal
Enzymes
Collagenase Type 1 : 0.05% - 0.06%
Medium
HEPES buffer
Liver
Rat
Rat, Fisher, adult, male
Williams, G. , Bermudez, E. and Scaramuzzino, D.
Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium ,
In Vitro
 13 (12) , 809 , 1977
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
HBSS
(see reference)
Liver
Rat
Rat, Fischer, male, adult, 170-265 g
Laishes, B. and Williams, G.
Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin ,
In Vitro
 12 , 521 , 1976
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%-0.10%
Medium
Williams E
Liver
Rat
Rat, SD, male, 200-250 g
Witters, L. , Alberico, L. and Acruch, J.
Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte ,
Biochem Biophys Res Commun
 69 (4) , 997 , 1976
Cells
Hepatocytes
Enzymes
Medium
MEM
Liver
Rat
Rat, embryos, 1-3 days
Gerschenson, L. , Berliner, J. and Davidson, M.
The Isolation and Culture of Liver Cells , Vol. 32 , 733 , 1974
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.10%
Medium
HBSS
Liver
Rat
Rat, Fischer, adult, 200-250 g
Williams, G. and Gunn, J.
Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
 89 , 139 , 1974
Cells
Epithelial
Enzymes
Hyaluronidase : 0.0075%
Medium
KCl-NaCl HEPES Buffer
Liver
Rat
Rat, neonate, 3 day
Bausher, J. and Schaeffer, W.
A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1 ,
In Vitro
 9 , 286 , 1974
Cells
Hepatocytes
Enzymes
Trypsin : 0.25%
Medium
PBS, CMF
Liver
Rat
Rat, SD, albino, male
Bonney, R. , Becker, J. , Walker, P. and Potter, V.
Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis ,
In Vitro
 9 , 399 , 1974
Cells
Parenchymal
Enzymes
Hyaluronidase : 0.1%
Medium
Hank's solution, CMF
Liver
Rat
Rat, SD, adult, male, 200-250 g
Howard, R. , Lee, J. and Pesch, L.
The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques ,
J Cell Biol
 57 , 642 , 1973
Cells
Parenchymal
Enzymes
Hyaluronidase : 0.10%
Medium
HBSS, CF
Liver
Rat
Rat, SD, adult, male, 180-300 g
Bissell, D. , Hammaker, L. and Meyer, U.
Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies ,
J Cell Biol
 59 , 722 , 1973
Cells
Parenchymal
Enzymes
Medium
Hank's solution, CF
Liver
Rat
Rat, Wistar, male, 260-310 g
Seglen, P.
Preparation of Rat Liver Cells ,
Exp Cell Res
 74 , 450 , 1972
Cells
Hepatocytes
Enzymes
Collagenase : 0.01 - 0.08%
Medium
HEPES
Liver
Rat
Rat, Wistar, 200-250 g
Berg, T. , Boman, D. and Seglen, P.O.
Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone ,
Exp Cell Res
 72 , 571 , 1972
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.10%
Medium
HBSS, CF
Liver
Rat
Rat, SD, female, 130-160 g
Johnson, M. , Das, N. , Butcher, F. and Fain, J.
The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids ,
J Biol Chem
 247 , 3229 , 1972
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.08%
Medium
HBSS, CF
Liver
Rat
Rat, Fisher 344, pregnant (19-21 days gestation),
200-250 g
Leffert, H. and Paul, D.
Studies on Primary Cultures of Differentiated Fetal Liver Cells ,
J Cell Biol
 52 , 559 , 1972
Cells
Hepatocytes
Enzymes
Collagenase : 0.3%
Medium
Modified Eagle's w/ Serum
Liver
Rat
Rat, Fisher, 10 day
Williams, G. , Weisburger, E. and Weisburger, J.
Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver ,
Exp Cell Res
 69 , 106 , 1971
Cells
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
PBS
Liver
Rat
Rat, Wistar, male, 6-8 weeks, 80-160 G
Iype, P.
Cultures from Adult Rat Liver Cells. 1.Establishment of Monolayer Cell-Cultures from Normal Lver ,
J Cell Physiol
 78 , 281 , 1971
Cells
Hepatocytes
Enzymes
Hyaluronidase : 1.0%
Medium
HBSS, CMF
Liver
Rat
Rat, 100-200 g
Haung, Y. and Ebner, K.
Induction of Tyrosine Aminotransferase in Isolated Liver Cells ,
Biochim Biophys Acta
 191 , 161 , 1969
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.10%
Medium
HBSS, CF
Liver
Rat
Rat, SD, adult, 200-300 g
Berry, M. and Friend, D.
High Yield Preparation of Isolated Rat Liver Parenchymal Cells ,
J Cell Biol
 43 , 506 , 1969
Cells
Parenchymal
Enzymes
Medium
HBSS, CF
Lung
Rat
Rat
Jones, R. , Capen, D. , Cohen, K. , Munn, L. , Jain, R. and Duda, D.
A Protocol for Phenotypic Detection and Characterization of Vascular Cells of Different Origins in a Lung Neovascularization Model in Rodents. ,
Nat Protoc
 3 , 388-97 , 2008
Cells
Lung
Enzymes
Medium
PBS
Lung
Rat
Rat, SD, 250-300g
Chen J. , Chen Z. , Narasaraju T. , Jin N. and Liu L.
Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs ,
Lab Invest
 84 , 727 , 2004
Cells
Alveolar epithelial
Enzymes
Elastase : 3-4.5 u/ml
Medium
RPMI 1640
Lung
Rat
Rat, SD, 125-150 g
Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.
Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
 52 , 759 , 2004
Cells
Alveolar type I & II
Enzymes
Elastase : 2.5-8 u/ml
Medium
DMEM/F12
Lung
Rat
Rat, SD, 300-400 g
King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F. and Stevens T
Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes ,
Microvasc Res
 67 , 139 , 2004
Cells
Pulmonary endothelial cells
Enzymes
Collagenase Type 2 : 1000 u/ml
Medium
DMEM/F-12
Lung
Rat
Rat, SD, 200-225 g
Sunil VR, Connor AJ, Guo Y, Laskin JD. and Laskin DL
Activation of type II alveolar epithelial cells during acute endotoxemia ,
Am J Physiol Lung Cell Mol Physiol
 282 , L872 , 2002
Cells
Type II alveolar epithelial cells
Enzymes
Elastase : 4.2 u/ml
Medium
DMEM
Lung
Rat
Rat, SD, male, adult
Kemp, P. , Kim, K. , Borok. and Crandall, E.
Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels ,
J Physiol
 536 (3) , 693 , 2001
Cells
Alveolar
Enzymes
Elastase : 2.0 - 2.5 u/ml
Medium
DMEM/Ham's F-12
(see reference)
Lung
Rat
Rat, Lewis, male, 200-250g
Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A.
Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages ,
Am J Respir Cell Mol Biol
 22 , 481-90 , 2000
Cells
Interstitial lung macrophages
Enzymes
Medium
RPMI-1640
Lung
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Lung
Rat
Rat, male, 200-250g
Bakhramov, A, Evans, M. and Kozlowski, R
Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
 83 , 337-47 , 1998
Cells
Pulmonary arterial myocytes
Enzymes
Collagenase : 0.15%
Papain : 0.15%
Elastase : 0.05%
Medium
PBS
Lung
Rat
Rat, embryonic day 15
Fraslon-Vanhulle, C, Bourbon, J. and Batenburg, J
Culture of Fetal Alveolar Epithelial Type II Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 13A:2.1 , 1995
Cells
Fetal alveolar epithelial type II
Enzymes
Trypsin : 0.1%
Medium
DMEM
Lung
Rat
Rat, SD, male, 125-175 g
Kim, K. , Suh, D. , Lubman, R. , Danto, S. , Borok, Z. and Crandall, E.
Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers ,
J Tiss Cul Meth
 14 , 187 , 1992
Cells
Alveolar epithelial
Enzymes
Elastase : 2 u/ml
Medium
EBSS
Lung
Rat
Rat, Wistar, female, virgin
Fraslon, C. , Rolland, G. , Bourbon, J. , Rieutort, M. and Valenza, C.
Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 843 , 1991
Cells
Alveolar epithelial type II
Enzymes
Trypsin : 1%
Medium
Eagle's MEM
Lung
Rat
Rat, Wistar
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS
Lung
Rat
Rat, SD, 8 day
Berk, J. , Franzblau, C. and Goldstein, R.
Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype ,
J Biol Chem
 266 , 3192 , 1991
Cells
Interstitial
Enzymes
Trypsin : 1.125%
Medium
HEPES buffer
Lung
Rat
Rat, Fischer 344, male
Mangum, J. , Everitt, J. , Bonner, J. , Moore, L. and Brody, A.
Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins ,
In Vitro Cell Dev Biol
 26 , 1135 , 1990
Cells
Alveolar type II pneumocytes
Enzymes
Elastase : 40 u/ml
Medium
HEPES buffer
(see reference)
Lung
Rat
Rat, SD, male, 250-400 g
Ma, J. , LaCagnin, L. , Bowman, L. and Miles, P.
Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells ,
Biochim Biophys Acta
 1003 , 136 , 1989
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
Phosphate-buffered medium
(see reference)
Lung
Rat
Rat, Wistar, adult, male and pregnant female (known gestation)
Batenburg, J. , Otto-Verberne, C. , Have-Opbroek, A. and Klazinga, W.
Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture ,
Biochim Biophys Acta
 960 , 441 , 1988
Cells
Alveolar type II
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.