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Tissue References Search Tool

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Muscle
Chicken
Chick, embryonic
Hilfer, S.
Collagenase Treatment of Chick Heart and Thyroid , Tissue Cult Methods & Applications , Kruse, P., and Patterson, M. , 246 , 1971
Cells
Muscle
Enzymes
Trypsin : 0.1%
Medium
CMF HBSS
Muscle
Chicken
Chick embryonic
Rinaldini, L.
An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells ,
Exp Cell Res
 16 , 477 , 1959
Cells
Various tissues (heart, liver, skeletal, cardiac)
Enzymes
Trypsin : various grades
Medium
CMF Tyrode's solution
Muscle
Ovine
Sheep, adult and neonatal
Driska S. , Laudadio R. , Wolfson M. and Shaffer T.
A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
 86(1) , 427 , 1999
Cells
Tracheal smooth muscle cells
Enzymes
Papain : 0.2%
Medium
MOPS-PSS
Neural
Chicken
Chick, White Leghorn, embryos
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Neural
Chicken
Chicken, White Leghorn, embryos, 17-21 day
Raman, I. and Trussell, L.
The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus ,
Neuron
 9 , 173 , 1992
Cells
Neurons
Enzymes
Papain : 40 u/ml
Medium
HEPES
Neural
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Neural
Chicken
Chick, White Leghorn, embryo
(also rat, SD, embryo)
Schnaar, R. and Schaffner, A.
Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions ,
J Neurosci
 1 , 204 , 1981
Cells
Spinal cord
Enzymes
Trypsin : 0.05%
Medium
Phosphate buffer
(see reference)
Neural
Chicken
Chick
Choi, D. and Fiscbach, G.
GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture ,
J Neurophysiol
 45 , 605 , 1981
Cells
Dorsal root ganglion neurons
Spinal cord
Enzymes
Trypsin : 0.1%
Medium
Puck's saline, CMF
Neural
Chicken
Chick, White Leghorn embryo, 8 day
Bottenstein, J. , Skaper, S. , Varon, S. and Sato, G.
Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium ,
Exp Cell Res
 125 , 183 , 1980
Cells
Neurons, ganglia
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Chicken
Chicken, White Leghorn, fertile eggs
Tuttle, J. , Suszkiw, J. and Ard, M.
Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture ,
Brain Res
 183 , 161 , 1980
Cells
Ciliary ganglion neurons
Enzymes
Trypsin : 0.25%
Medium
Eagle's MEM
Neural
Chicken
Chicken, embryos, 9-10 days old
Mudge, A. , Leeman, S. and Fischbach, G.
Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration ,
Proc Natl Acad Sci U S A
 76 (1) , 526 , 1979
Cells
Dorsal root ganglia neurons
Enzymes
Collagenase : 0.01%
Medium
Eagle's MEM
Neural
Chicken
Chick embryos
McCarthy, K. and Partlow, L.
Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates ,
Brain Res
 114 , 391 , 1976
Cells
Ganglion chains, sympathetic ganglia
Enzymes
Trypsin : 0.25%
Medium
Krebs Phosphosaline
Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Neural
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Reproductive
Chicken

Chicken, male, 1 day

Huang, H. , Wang, M. , Hou, L. , Lin, X. , Pan, S. , Zheng, P. and Zhao, Q.
A Potential Mechanism Associated with Lead-induced Spermatogonia and Leydig Cell Toxicity and Mitigative Effect of Selenium in Chicken. , Ecotoxicol Environ Saf Vol. 209 , 111671 , 2021
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Trypsin : 0.1%
Medium
DMEM
Reproductive
Chicken
Chicken, Gallus Domesticus,20-30 week
Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong.
An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles ,
J Reprod Dev
 52 , 569-76 , 2006
Cells
Primary follicles
Enzymes
Trypsin : 0.15%
Medium
Dulbecco's phosphate buffered saline
Reproductive
Ovine
Sheep
Salamonsen, L. , Sum O, W. , Doughton, B. and Findlay, J.
The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium ,
Endocrinology
 117 (5) , 2148 , 1985
Cells
Epithelial
Enzymes
Collagenase : 125 - 190 u/ml
Medium
DMEM
Thyroid/Parathyroid
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Thyroid/Parathyroid
Chicken
Chicken, Rhode Island Red, embryo
Spooner, B.
The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture ,
J Cell Physiol
 75 , 33 , 1970
Cells
Thyroid follicular
Enzymes
Collagenase : 0.2%
Medium
Tyrode's solution, CMF
Thyroid/Parathyroid
Ovine
Sheep
Kerkof, P.
Preparation of Primary Cultures of Ovine Thyroid Gland Cells ,
J Tiss Cul Meth
 7 , 23 , 1982
Cells
Thyroid
Enzymes
Collagenase : 0.2%
Medium
Puck's Saline F

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.