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Tissue References Search Tool

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Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adipose/Fat
Bear
Bear, Ursus arctos
Gehring, J. , Rigano, K. , Evans, H. , Nelson, O. , Robbins, C. and Jansen, H.
A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes. ,
Cytotechnology
 68 , 2177-91 , 2016
Cells
Adipocytes
Enzymes
Medium
HBSS
Epithelial
Frog
Frog, Xenopus laevis, adult, female
Heinke, B. and Clauss, W.
Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium ,
J Comp Physiol [B]
 169 , 148 , 1999
Cells
Colonic epithelial
Enzymes
Medium
Kreb's
Heart
Frog
Frog (Rana esculenta)
Arrio-Dupont, M. and de Nay, D.
High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles ,
Biol Cell
 54 , 164 , 1985
Cells
Myocytes
Enzymes
Trypsin : 0.04%
Medium
CF Ringer
Kidney
Avian
Avian, house sparrows
Goldstein D. , Reddy V. and Plaga K.
Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones ,
Am J Physiol
 276 , R847 , 1999
Cells
Tubule segments
Enzymes
Medium
HBSS
Kidney
Avian
Avian, chicken
Reich, C. and Bonar, R.
Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture ,
Meth Cell Sci
 7 , 97 , 1982
Cells
Tubule and glomeruli
Enzymes
Medium
RPMI 1640
Liver
Avian
Avian, various
Else, P. , Brand, M. , Turner, N. and Hulbert, A
Respiration Rate of Hepatocytes Varies With Body Mass in Birds ,
J Exp Biol
 207 , 2305 , 2004
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Avian
Ducklings, 7 day
Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.
Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
 75(16) , 7651 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.05%
Medium
DMEM/ F12
Muscle
Frog
Frog, Xenopus laevis, embryos stage 17 & 19
Stollberg, J. and Fraser, S.
Acetylcholine Receptors and Con A-Binding Sites on Xenopus Muscle Cells ,
J Cell Biol
 107 , 1397 , 1988
Cells
Muscle
Enzymes
Collagenase : 0.10%
Medium
Steinberg's solution
Muscle
Frog
Frog
Shepherd, N. and Kavaler, F.
Direct Control of Contraction Force of Single Frog Atrial Cells by Extracellular Ions ,
Am J Physiol
 251 , C653 , 1986
Cells
Myocytes
Enzymes
Trypsin : 0.1%
Medium
CF Ringer
Muscle
Frog
Frog, Rana pipiens (50-100 g)
(also guinea-pig, rabbit, dogfish, Squalus acanthias )
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Frog
Frog, Xenopus laevis
Anderson, M.J. , Cohen, M.W. and Zorychta, E.
Effects of Innervation on the Distribution of Acetylcholine Receptors on Cultured Muscle Cells ,
J Physiol
 268 , 731 , 1977
Cells
Muscle
Enzymes
Trypsin : 0.5%
Medium
L15 medium
(see reference)
Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Avian
Chick, White Leghorn, embryos
Temburni, M. , Rosenberg, M. , Pathak, N. , McConnell, R. and Jacob, M.
Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein. ,
J Neurosci
 24 , 6776 , 2004
Cells
Cilary ganglion neurons
Enzymes
Medium
PBS
Neural
Frog
Xenopus, embryonic
Takahashi, T. , Nakajima, Y. , Hirosawa, K. , Nakajima, S. and Onodera, K.
Structure and Physiology of Developing Neuromuscular Synapses in Culture ,
J Neurosci
 7 , 473 , 1987
Cells
Neuron
Enzymes
Medium
Steinberg's solution
Reproductive
Frog

Frog, X. laevis

Lavering, E. , Petros, I. and Weeks, D.
Component Analysis of Nucleolar Protein Compartments using Xenopus laevis Oocytes. , Dev Growth Differ Vol. 64 , 306-317 , 2022
Cells
Xenopus oocytes
Enzymes
Medium
Ovary Ringers Solution
Reproductive
Frog
Xenopus Laevis, female
Nicoll, G, Jawad, A. , Weymouth, R, Zhang, H. and Beg, A.
Pharmacological characterization of the excitatory ‘Cys-loop’ GABA receptor family in Caenorhabditis elegans ,
Br J Pharmacol
 174 , 781 , 2017
Cells
Oocytes
Enzymes
Medium
Barth's solution
Reproductive
Frog
Xenopus laevis
Chatzidaki, A. , D'Oyley, J. , Gill-Thind, J. , Sheppard, T. and Millar, N.
The Influence of Allosteric Modulators and Transmembrane Mutations on Desensitisation and Activation of alpha7 Nicotinic Acetylcholine Receptors. ,
Neuropharmacol
 97 , 75-85 , 2015
Cells
Oocytes
Enzymes
Collagenase : 0.2%
Medium
Barth's solution
Reproductive
Frog
Xenopus
O'Connell, D. , Mruk, K. , Rocheleau, J. and Kobertz, W.
Xenopus laevis Oocytes Infected with Multi-drug-resistant Bacteria: Implications for Electrical Recordings. ,
J Gen Physiol
 138 , 271-7 , 2011
Cells
Oocytes
Enzymes
Medium
see reference
Reproductive
Frog
Xenopus laevis, female
Cohen, S. , Au, S. and Pante, N.
Microinjection of Xenopus Laevis Oocytes. ,
J Vis Exp
 24 , 1106 , 2009
Cells
Oocytes
Enzymes
Collagenase : 0.5%
Medium
Barth's solution, CF
Reproductive
Frog
Xenopus laevis, female
Mruk, K. and Kobertz, W.
Discovery of a Novel Activator of KCNQ1-KCNE1 K Channel Complexes. ,
PLoS ONE
 4 , e4236 , 2009
Cells
Oocytes
Enzymes
Collagenase : 0.2%
Medium
see reference
Reproductive
Frog
Xenopus laevis, female
Pannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio.
Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species ,
J Biol Chem
 277 , 8912-9 , 2002
Cells
Oocytes
Enzymes
Medium
see reference
Reproductive
Frog
Xenopus laevis, female
Alagem, N. , Dvir, M. and Reuveny, E.
Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues ,
J Physiol
 534 (2) , 381 , 2001
Cells
Oocytes
Enzymes
Medium
CF Medium
Reproductive
Frog
Xenopus
Tian, J. , Kim, S. , Heilig, E. and Ruderman, J.
Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation ,
Proc Natl Acad Sci U S A
 97 , 14358 , 2000
Cells
Oocytes
Enzymes
Medium
(see reference)
Reproductive
Frog
Xenopus laevis, female
Karkanias, N. and Papke, R.
Subtype-Specific Effects of Lithium on Glutamate Receptor Function ,
J Neurophysiol
 81 , 1506 , 1999
Cells
Oocytes
Enzymes
Collagenase : 0.1%
Medium
Barth's solution, CF
Reproductive
Frog
Xenopus laevis, mature female
Moriarty, T. , Gillo, B. , Carty, D. , Premont, R. , Landau, E. and Iyengar, R.
Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C ,
Proc Natl Acad Sci U S A
 85 , 8865 , 1988
Cells
Oocytes
Enzymes
Collagenase : 0.2%
Medium
CF Medium
Skin
Frog
Frog
Nishikawa, A. , Shimizu-Nishikawa, K. and Miler, L.
Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis ,
In Vitro Cell Dev Biol
 26 , 1128 , 1990
Cells
Epidermal
Enzymes
Trypsin : 0.18%
Medium
Barth's solution, CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.