For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Liver
Human
Human
Werner, M. , Driftmann, S. , Kleinehr, K. , Kaiser, G. , Mathe, Z. , Treckmann, J. , Paul, A. , Skibbe, K. , Timm, J. , Canbay, A. , Gerken, G. , Schlaak, J. and Broering, R.
All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. ,
PLoS ONE
 10 , e0138655 , 2015
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Human
Human
Lee, S. , Schelcher, C. , Demmel, M. , Hauner, M. and Thasler, W.
Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. ,
J Vis Exp
 , 2013
Cells
Hepatocytes
Enzymes
Collagenase : 0.1-0.15%
Medium
HEPES
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Liver
Human
Human
Torok, E. , Lutgehetmann, M. , Bierwolf, J. , Melbeck, S. , Dullmann, J. , Nashan, B. , Ma, P. and Pollok, J.
Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering. ,
Liver Transpl
 17 , 104 , 2011
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Wauthier, E. , Schmelzer, E. , Turner, W. , Zhang, L. , LeCluyse, E. , Ruiz, J. , Turner, R. , Furth, M. , Kubota, H. , Lozoya, O. , Barbier, C. , McClelland, R. , Yao, H. , Moss, N. , Bruce, A. , Ludlow, J. and Reid, L.
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. ,
Methods Cell Biol
 86 , 137 , 2008
Cells
Hepatic stem cells and heptoblasts
Enzymes
Collagenase Type 4 : 0.014-0.06%
Medium
various
Liver
Human
Human
Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC. and Maurel P
Human hepatocyte culture ,
Methods Mol Biol
 320 , 283 , 2006
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES
Liver
Human
Human
Hughes, R. , Mitry, R. , Dhawan, A. , Lehec, S. , Girlanda, R. , Rela, M. , Heaton, N. and Muiesan, P.
Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation ,
Liver Transpl
 12 , 713 , 2006
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
EBSS
Liver
Human
Human
Hussain, S. , Strom, S. , Kirby, M. , Burns, S. , Langemeijer, S. , Ueda, T. , Hsieh, M. and Tisdale, J.
Side Population Cells Derived from Adult Human Liver Generate Hepatocyte-Like Cells In Vitro. ,
Dig Dis Sci
 50 , 1755 , 2005
Cells
Hepatic side population
Enzymes
Collagenase : 0.02-0.05%
Medium
HBSS
Liver
Human
Human, adult
Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R. and Gupta S.
  Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications ,
Liver Int
 24 , 361 , 2004
Cells
Hepatocytes
Enzymes
Collagenase : 0.6%
Medium
RPMI 1640
Liver
Human
Human, fetal
Malhi, H. , Irani, A. , Gagandeep, S. and Gupta, S.
Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes. ,
J Cell Sci
 115 , 2679 , 2002
Cells
Epithelial progenitor
Enzymes
Collagenase : 0.03%
Medium
DMEM
Liver
Human
Human
Duanmu Z. , Locke D. , Smigelski J. , Dahn M. , Falany C. , Kocarek T. and Runge-Morris M.
Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes ,
Drug Metab Dispos
 30(9) , 997 , 2002
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
(also rat)
Dandri, M. , Burda, M. , Torok, E. , Pollok, J. , Iwanska, A. , Sommer, G. and et al.
Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus ,
Hepatol
 33 , 981 , 2001
Cells
Hepatocytes
Enzymes
Medium
Leffert's buffer
Liver
Human
Human
Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O.
CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers ,
Drug Metab Dispos
 28 , 1321-6 , 2000
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Human
Human
Parzefall, W. , Erber, E. , Sedivy, R. and Schulte-Hermann, R.
Testing for Induction of DNA Synthesis in Human Hepatocyte Primary Cultures by Rat Liver Tumor Promoters ,
Cancer Res
 51 , 1143 , 1991
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Gomez-Lechon, M. , Lopez, P. , Donato, T. , Montoya, A. , Larrauri, A. , Gimenez, P. , Trullenque, R. , Fabra, R. and Castell, J.
Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo ,
In Vitro Cell Dev Biol
 26 , 67 , 1990
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES buffer
(see reference)
Liver
Human
Human, male, 17-40 yrs.
Begue, J. , Baffet, G. , Campion, J. and Guillouzo, A.
Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3 ,
Biol Cell
 63 , 327 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES buffer
Liver
Human
Human, 4 male kidney donors, age between 17 and 31
(also adult rats, male, SD, 180-200 g)
LeBot, M. , Begue, J. , Kernaleguen, D. , Robert, J. , Ratanasavanh, D. , Airiau, J. , Riche, C. and Guillouzo, A.
Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes ,
Biochem Pharmacol
 37 (20) , 3877 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05% & 0.025%
Medium
HEPES
(see reference)
Liver
Human
Human, 3 human kidney donors, age 20, 23, 25
Gugen-Guillouzo, C. , Campion, J. , Brissot, P. , Glaise, D. , Launois, B. , Bourel, M. and Guillouzo, A.
High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver ,
Cell Biol Int Rep
 6 (6) , 625 , 1982
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES
Liver
Human
Human, 51-75 years old
(also rat, guinea-pig, rabbit, dog)
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Human
Human
Kaighn, M.
Human Liver Cells , Tissue Culture Methods / Applications , (Kruse, P., Patterson, M. eds) , 54 , 1973
Cells
Hepatocytes
Enzymes
Trypsin : 0.1%
Medium
HBSS, CMF
Lung
Guinea-Pig
Guinea-pig, male, 250-300 g
Sikpi, M. , Nair, C. , Johns, A. and Das, S.
Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation ,
Biochim Biophys Acta
 877 , 20 , 1986
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
PBS
(see reference)
Lung
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Lung
Human

Human

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Human

Human

Youk, J. , Kim, T. , Evans, K. , Jeong, Y. , Hur, Y. , Hong, S. , Kim, J. , Yi, K. , Kim, S. , Na, K. , Bleazard, T. , Kim, H. , Fellows, M. , Mahbubani, K. , Saeb-Parsy, K. , Kim, S. , Kim, Y. , Koh, G. , Choi, B. , Ju, Y. and Lee, J.
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. , Cell Stem Cell Vol. 27 , 905-919.e10 , 2020
Cells
Aveolar
Enzymes
Medium
DMEM
Lung
Human
Human
Bahmed, K. , Boukhenouna, S. , Karim, L. , Andrews, T. , Lin, J. , Powers, R. , Wilson, M. , Lin, C. , Messier, E. , Reisdorph, N. , Powell, R. , Tang, H. , Mason, R. , Criner, G. and Kosmider, B.
The Effect of Cysteine Oxidation on DJ-1 Cytoprotective Function in Human Alveolar Type II Cells. ,
Cell Death Dis
 10 , 638 , 2019
Cells
Aveolar
Enzymes
Elastase : 12.9 u/ml
Medium
PBS
Lung
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Human
Human
Mackay, L. , Dodd, S. , Dougall, I. , Tomlinson, W. , Lordan, J. , Fisher, A. and Corris, P.
Isolation and Characterisation of Human Pulmonary Microvascular Endothelial Cells from Patients with Severe Emphysema. ,
Respir Res
 14 , 23 , 2013
Cells
Pulmonary endothelial
Enzymes
Medium
RPMI
Lung
Human
Human
Comhair, S. , Xu, W. , Mavrakis, L. , Aldred, M. , Asosingh, K. and Erzurum, S.
Human Primary Lung Endothelial Cells in Culture. ,
Am J Respir Cell Mol Biol
 46 , 723-30 , 2012
Cells
Lung endothelial
Enzymes
Medium
PBS
Lung
Human
Human
Fujino, N. , Kubo, H. , Suzuki, T. , Ota, C. , Hegab, A. , He, M. , Suzuki, S. , Suzuki, T. , Yamada, M. , Kondo, T. , Kato, H. and Yamaya, M.
Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs. ,
Lab Invest
 91 , 363 , 2011
Cells
Lung
Enzymes
Medium
DMEM
Lung
Human
Human
Zhu, Y. , Skold, C. , Liu, X. , Wang, H. , Kohyama. , Wen, F. , Ertl, R. and Rennard, S.
Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture ,
Respir Res
 2 (5) , 295 , 2001
Cells
Lung
Fibroblasts
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Human
Human, 16-24 week therapeutic abortions
Liley, H. , Ertsey, R. , Gonzales, L. , Odom, M. , Hawgood, S. , Dobbs, L. and Ballard, P.
Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung ,
Biochim Biophys Acta
 961 , 86 , 1988
Cells
Alveolar type II
Fetal
Enzymes
Trypsin : 50 ug/ml
Medium
Ham's F-12, Eagle's MEM
Lung
Human
Human
Lechner, J. , Haugen, A. , McClendon, I. and Pettis, E.
Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium ,
In Vitro
 18 (7) , 633 , 1982
Cells
Epithelial
Enzymes
Trypsin : 0.02%
Medium
Medium 199
Lung
Human
Human, 12-16 wk old embryo
Kan, M. and Yamane, I.
In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium ,
J Cell Physiol
 111 , 155 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.01%
Medium
Eagle's MEM
Lung
Human
Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Mammary
Guinea-Pig
Guinea-pig, pregnant, 4-10 day
Turba, F. and Hilpert, N.
Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German ,
Biochem Z
 334 , 501 , 1961
Cells
Mammary gland
Enzymes
Medium
Dulbecco phosphate

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.