For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Muscle
Guinea-Pig
Guinea-pig
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate
Muscle
Guinea-Pig
Guinea pig, 2-4 wk old, male, female
Firth, T. , Mawe, G. and Nelson, M.
Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle ,
Am J Physiol Cell Physiol
 278 , C1031 , 2000
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
Krebs solution
Muscle
Guinea-Pig
Guinea-pig, 200-380 g
Schnitzler, M. , Derst, C. , Daut, J. and Preisig-Muller, R.
ATP-Sensitive Potassium Channels in Capillaries Isolated From Guinea-Pig Heart ,
J Physiol
 525 (2) , 307 , 2000
Cells
Capillaries
Myocytes
Enzymes
Medium
CF solution
Muscle
Guinea-Pig
Guinea-pig, adult, 250-350 g
Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.
Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle ,
Am J Physiol
 277 , G1017 , 1999
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES
Muscle
Guinea-Pig
Guinea-pig, Dunkin-Hartley, female
Ryder, K. , Bryant, S. and Hart, G.
Membrane Current Changes in Left Ventricular Myocytes Isolated From Guinea-Pigs After Abdominal Aortic Coarctation ,
Cardiovasc Res
 27 , 1278 , 1993
Cells
Myocytes
Medium
DMEM
Muscle
Guinea-Pig
Guinea-pig
(also rat, rabbit)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Guinea-Pig
Guinea-pig, 200-300 g
Ohya, Y. and Sperelakis, N.
ATP Regulation of the Slow Calcium Channels in Vascular Smooth Muscle Cells ,
Circ Res
 64 , 145 , 1989
Cells
Smooth muscle
Mesenteric artery
Enzymes
Collagenase : 0.3%
Medium
CF solution
Muscle
Guinea-Pig
Guinea-pig, 200-400 g
(also rabbit, frog, dogfish)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Guinea-Pig
Guinea-pig, prepubertal
Ross, R.
The Smooth Muscle Cell . II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers ,
J Cell Biol
 50 , 172 , 1971
Cells
Smooth muscle
Aortic
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt modification of Eagle's
Muscle
Rabbit
Rabbit, New Zealand
Al-Shboul, O. , Mahavadi, S. , Sriwai, W. , Grider, J. and Murthy, K.
Differential Expression of Multidrug Resistance Protein 5 and Phosphodiesterase 5 and Regulation of cGMP Levels in Phasic and Tonic Smooth Muscle. ,
Am J Physiol Gastrointest Liver Physiol
 305 , G314-24 , 2013
Cells
Gastric smooth muscle
Enzymes
Medium
DMEM
Muscle
Rabbit
Rabbit, New Zealand White
Croons Valerie, Martinet Wim, Herman Arnold G, Timmermans Jean-Pierre, De Meyer Guido R Y.
Selective clearance of macrophages in atherosclerotic plaques by the protein synthesis inhibitor cycloheximide ,
J Pharmacol Exp Ther
 320 , 986-93 , 2007
Cells
Aortic smooth muscle
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham's
Muscle
Rabbit
Rabbit, New Zealand, male, 2.5-3.5 Kg
Santos, M. , Nguyen, B.. and Thompson, J.
Factors Affecting in Vitro Growth of Harvested Enterocytes ,
Cell Transplant
 1 , 299 , 1992
Cells
Enterocytes
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640 w/ 1% fetal bovine serum
PBS
Muscle
Rabbit
Rabbit
(also rat, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rabbit
Rabbit, adult, 1-2 kg
Benham, C. , Bolton, T. , Byrne, N. and Large, W.
Action of Extremely Applied Adenosine Triphosphate O Single Smooth Muscle Cells Dispersed From Rabbit Ear Artery ,
J Physiol
 387 , 473 , 1987
Cells
Smooth muscle
Enzymes
Elastase : 0.17 - 0.25%
Medium
Saline
Muscle
Rabbit
Rabbit, New Zealand white, 1500 g
Knodle, S. , Anderson, S. and Papaioannou, S.
Large Scale Preparation of Rabbit Aortic Smooth Muscle Cells For Use in Calcium Uptake Studies ,
In Vitro Cell Dev Biol
 22 , 23 , 1986
Cells
Smooth muscle, aortic
Enzymes
Trypsin : 0.038%
Medium
MEM
Muscle
Rabbit
Rabbit, adult, 1-2 Kg
Benham, C.D. and Bolton, T.B.
Spontaneous Transient Outward Currents in Single Visceral and Vascular Smooth Muscle Cells of the Rabbit ,
J Physiol
 381 , 385 , 1986
Cells
Smooth muscle, ear artery
Enzymes
Trypsin : 0.1%
Medium
CF solution
(see reference)
Muscle
Rabbit
Rabbit, 0.5-1 Kg
(also guinea-pig, frog, dogfish)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Rabbit
Rabbit, white New Zealand, adult, male, 2 Kg
Ives, H. , Schultz, G. , Galardy, R. and Jamieson, J.
Preparation of Functional Smooth Muscle Cells from the Rabbit Aorta ,
J Exp Med
 148 , 1400 , 1978
Cells
Smooth muscle, aorta
Enzymes
Trypsin : 0.1%
Medium
Krebs Ringer HEPES solution
Muscle
Rabbit
Rabbits, New Zealand white albino, 5-6 months
(also human, Rhesus monkey, rabbit)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Muscle
Rabbit
Rabbit, chinchilla, 5-8 month, virgin, female, 2-3 kg
Peters, T. , Muller, M. and deDuve, C.
Lysosomes of the Arterial Wall. I. Isolation and Subcellular Fractionation of Cells from Normal Rabbit Aorta ,
J Exp Med
 136 , 1117 , 1972
Cells
Smooth muscle, aorta
Enzymes
Hyaluronidase : 800 u/ml
Medium
HBSS
Muscle
Rabbit
Rabbit, New England, albino
Day, A. , Phil, D. and Newman, H.
Synthesis of Phospholipid by Foam Cells Isolated from Rabbit Atherosclerotic Lesions ,
Circ Res
 XIX , 122 , 1966
Cells
Thoracic aorta
Enzymes
Elastase : 0.008%
Medium
Kreb's Ringer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.