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Tissue References Search Tool

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Adipose/Fat
Hamster
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, 5 week-12 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Hamster
Hamster (Mesocricetus auratus) 100-150 g
Liang, B.T. and Perlman, R.L.
Catecholamine Secretion by Hamster Adrenal Cells ,
J Neurochem
 32 , 927 , 1979
Cells
Adrenal
Chromaffin
Enzymes
Hyaluronidase : 0.20%
Medium
Kreb's Ringer bicarbonate buffer
Brain
Shellfish
Helisoma trivolvis
Hadley, R.D. , Bodnar, D.A. and Kater, S.B.
Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation ,
J Neurosci
 5 , 3145 , 1985
Cells
Neurons
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Saline, Sterile
Epithelial
Hamster
Hamster, Syrian gold, male, 100-120 g
Niles,R. , Kim, K.C. , Hyman, B. , Christensen, T. , Wasano, K. and Brody, J.
Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells ,
In Vitro Cell Dev Biol
 24 , 457 , 1988
Cells
Tracheal
Enzymes
Pronase : 0.1%
Medium
MEM with Hepes, CMF
Epithelial
Hamster
Hamster, Syrian golden (strain CR:RGH)
McDowell, E. and et al.
Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters ,
Am J Pathol
 129 , 511 , 1987
Cells
Tracheal
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12
Epithelial
Hamster
Hamster (strain 1516 EHS and Lakeview), 8-12 weeks
Lee, T. , Wu, R. , Brody, A. , Barrett, J. and Nettesheim, P.
Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture ,
Exp Lung Res
 6 , 27 , 1984
Cells
Tracheal
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Epithelial
Hamster
Hamster, Syrian gold, male, 6 weeks- 4 months
Goldman, W.E. and Baseman, J.B.
Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea ,
In Vitro
 16 , 313 , 1980
Cells
Tracheal
Enzymes
Trypsin : 0.25%
Medium
PBS with EDTA
Heart
Shellfish
Pacific Oysters
Le Duff, R. , Lipart, C. and Renault, T.
Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells ,
J Tiss Cul Meth
 16 , 67 , 1994
Cells
Haemocytes
Medium
L15 medium
Heart
Shellfish
Clams, 3.0 to 4.5 cm
Wen, C. , Kou, G. and Chen, S.
Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding) ,
J Tiss Cul Meth
 15 , 123 , 1993
Cells
Myocytes
Fibroblasts
Enzymes
Medium
L15 medium
Kidney
Hamster
Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)
Husted, R. , Hayashi, M. and Stokes, J.
Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
 255 , F1160 , 1988
Cells
Papillary duct
Enzymes
Hyaluronidase : 0.2%
Medium
Keri's buffer
HEPES buffered saline
Muscle
Hamster
Hamster, male, 60-70 day
Nakamura, T. , Iwata, Y. , Sampaolesi, M. , Hanada, H. , Saito, N. , Artman, M. , Coetzee, W. and Shigekawa, M.
Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters ,
Am J Physiol Cell Physiol
 281 , C690 , 2001
Cells
Satellite
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Quail
Quail, embryo, 10 day
Konigsberg, I.
Skeletal Myoblasts in Culture ,
Methods Enzymol
 LVIII , 511 , 1979
Cells
Myoblasts
Enzymes
Medium
Puck's solution
Neural
Hamster
Hamster, male
Liman ER.
Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons ,
J Physiol
 548 , 777 , 2003
Cells
Vomeronasal organ neurons
Enzymes
Trypsin : 0.02%
Medium
PBS
Neural
Quail
Quail
Sieber-Blum, M. and Cohen, A.
Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells ,
Dev Biol
 80 , 96 , 1980
Cells
Neural crest
Enzymes
Trypsin : 0.05%
Medium
MEM, HBSS
Neural
Shellfish
Aplysia californica
Lee, A. , Decourt, B. and Suter, D.
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones. ,
J Vis Exp
 12 , 662 , 2008
Cells
Neurons
Enzymes
Medium
L-15-ASW
Neural
Shellfish
Snails (Helisoma trivolis), albino, adult
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Neural
Shellfish
Pond snail (Helisoma) albino, adult
Haydon, P.
The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata ,
J Neurosci
 8 , 1032 , 1988
Cells
Somata, buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Antibiotic saline, Leibowitz 50%
Neural
Shellfish
Helisoma trivovlis
Cohan, C. , Haydon, P. and Kater, S.
Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma ,
J Neurosci Res
 13 , 285 , 1985
Cells
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
L-15 medium
Neural
Shellfish
Mollusc, juvenile 1-2 gm or adult 50-100 gm
Schacher, S. and Proshanksy, E.
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment ,
J Neurosci
 3 (12) , 2403 , 1983
Cells
Neurons
LUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Shellfish
Aplysia californica
Camardo, J. , Proshansky, E. and Schacher, S.
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture ,
J Neurosci
 3 (12) , 2614 , 1983
Cells
LUQ cells
RUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Pancreas
Hamster
Hamster
Feldman, J. and Chapman, B.
Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique ,
Acta Diabetol
 12 , 208 , 1975
Cells
Islets
Enzymes
Collagenase Type 4 : 1.3% - 2.0%
Medium
HBSS
Reproductive
Hamster
Hamster, Chinese
Wallis, R. and Drickamer, K.
Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins ,
J Biol Chem
 274 (6) , 3580 , 1999
Cells
Ovary
Enzymes
Trypsin : 5%
Medium
Dialyzed fetal calf serum, 10% and 0.5M Methotrexate
Tumor
Hamster
Hamster, 90-100 g
Gonzalez, A. , Oberley, T. , Schultz, J. , Ostrom, J. and Li, J.
In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney ,
In Vitro Cell Dev Biol
 29A , 562 , 1993
Cells
Tumor
Enzymes
Hyaluronidase : 0.1%
Medium
Waymouth's MB
Tumor
Hamster
Hamster, 6 week old
Min, B. , Kim, K. , Cherrick, H. and Park, N.
Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation ,
In Vitro Cell Dev Biol
 27A , 128 , 1991
Cells
Buccal pouch
Enzymes
Medium
CMF HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.