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Tissue References Search Tool

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Eye
Porcine
Porcine, adult
Xu, N. , Chen, Y. , Dean, K. , Lu, X. , Liu, X. , Wang, W. , Dean, D. , Kaplan, H. , Gao, L. , Dong, F. and Liu, Y.
Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. ,
Stem Cells
 35 , 1579-1591 , 2017
Cells
Muller glia
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Porcine
Porcine, 5-60 kg
Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M.
Growth of Cultured Porcine Retinal Pigment Epithelial Cells ,
Acta Opthalmol Scand
 81(2) , 170 , 2003
Cells
Retinal pigment epithelial cells
Enzymes
Medium
DMEM
Heart
Human
Human
Holt-Casper, D. , Theisen, J. , Moreno, A. , Warren, M. , Silva, F. , Grainger, D. , Bull, D. and Patel, A.
Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds. ,
J Transl Med
 13 , 194 , 2015
Cells
Cardiac
Enzymes
Collagenase : 0.045%
Pancreatin : 0.1%
Medium
DPBS
Heart
Human
Human
Sharma, S. , Mishra, R. , Simpson, D. , Wehman, B. , Colletti, E. , Deshmukh, S. , Datla, S. , Balachandran, K. , Guo, Y. , Chen, L. , Siddiqui, O. , Kaushal, S. and Kaushal, S.
Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome. ,
Stem Cells
 33 , 1213-29 , 2015
Cells
Cardiospheres
Enzymes
Medium
Iscove modified DMEM
Heart
Human
Human
Avolio, E. , Gianfranceschi, G. , Cesselli, D. , Caragnano, A. , Athanasakis, E. , Katare, R. , Meloni, M. , Palma, A. , Barchiesi, A. , Vascotto, C. , Toffoletto, B. , Mazzega, E. , Finato, N. , Aresu, G. and Livi, U.
Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. ,
Stem Cells
 32 , 2373 , 2014
Cells
Cardiac stem
Enzymes
Medium
Joklik modified Eagle’s
Heart
Human
Human
Voigt, N. , Zhou, X. and Dobrev, D.
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. ,
J Vis Exp
 , e50235 , 2013
Cells
Atrial myocytes
Enzymes
Collagenase Type 1 : 286 u/ml
Protease : 5 u/ml
Medium
see references
Heart
Human
Human
Vukusic, K. , Jonsson, M. , Brantsing, C. , Dellgren, G. , Jeppsson, A. , Lindahl, A. and Asp, J.
High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis. ,
Biomed Res Int
 2013 , 2013
Cells
Cardiac
Enzymes
Medium
DMEM
Heart
Human
Human
Anderson, E. , Rodriguez, E. , Anderson, C. , Thayne, K. , Chitwood, W. and Kypson, A.
Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways. ,
Am J Physiol Heart Circ Physiol
 300 , H118 , 2011
Cells
Myofibers
Enzymes
Medium
see reference
Heart
Human
Human
Voigt, N. , Makary, S. , Nattel, S. and Dobrev, D.
Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart. ,
Methods Enzymol
 484 , 653 , 2010
Cells
Atrial cardiomyocytes
Enzymes
Collagenase Type 1 : 286 u/ml
Protease : 5 u/ml
Medium
see reference
Heart
Human
Human
Jensen, B. , Swigart, P. , Laden, M. , DeMarco, T. , Hoopes, C. and Simpson, P.
The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries. ,
J Am Coll Cardiol
 54 , 1137 , 2009
Cells
Coronary artery smooth muscle
Enzymes
Elastase : 0.05%
Medium
DMEM
Heart
Human
Human
Mathew, S. , Tustison, K. , Sugatani, T. , Chaudhary, L. , Rifas, L. and Hruska, K.
The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD. ,
J Am Soc Nephrol
 19 , 1092 , 2008
Cells
Smooth muscle aortic
Enzymes
Medium
DMEM
Heart
Human
Human (n=16, age: 60 &plusmin;3 years)
Mukerjee, R. , Multani, M. , Sample, J. , Dowdy, K. , Zellner, J. , Hoover, D. and Spinale, F.
Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response ,
J Cardiovasc Pharmacol
 7 (4) , 235 , 2002
Cells
Myocardial
Enzymes
Hyaluronidase : 0.05%
Medium
Medium 199
Heart
Human
Human
Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N.
Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 283 , H990-5 , 2002
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.013%
Medium
HEPES solution
Heart
Human
Human
Shirai, T. , Rao, V. , Weisel, R. , Ikonomidis, J. , Li, R. , Tumiati, L. , Merante, F. and Mickle, D.
Preconditioning Human Cardiomyocytes and Endothelial Cells. ,
J Thorac Cardiovasc Surg
 115 , 210-9 , 1998
Cells
Cardiomyocytes and endothelial
Enzymes
Trypsin : 0.2%
Collagenase : 0.1%
Medium
PBS
Heart
Human
Human
Hoppe, U. , Jansen, E. , Sudkamp, M. and Beukelmann, D.
Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts ,
Circulation
 97 , 55 , 1998
Cells
Heart
Myocytes
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
Tyrode's solution
Heart
Human
Human
Van Wagoner, D. , Pond, A. , McCarthy, P. , Trimmer, J. and Nerbonne, J.
Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation. ,
Circ Res
 80 , 772-81 , 1997
Cells
Atrial myocytes
Enzymes
Protease Type XIV : 0.04%
Medium
see reference
Heart
Human
Human, fetal, 14.5 week gestation
Goldman, B. and Wurzel, J.
Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes ,
In Vitro Cell Dev Biol
 28 , 109 , 1992
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS with Calcium
Heart
Human
Human, both sexes, 18-81 years
Smith, D. , Glover, J. , Townsend, L. and Maupin, E.
A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor ,
In Vitro Cell Dev Biol
 27 , 914 , 1991
Cells
Myocardial
Atrial
Enzymes
Collagenase : 0.14%
Medium
HBSS, CMF
Heart
Human
Human, adult
Bugaisky, L.B.
Biology of Isolated Adult Cardiac Myocytes , Isolation and Culture of Human Adult Cardiac Myocytes , (Clark,Decker,Borg,eds) , Elsevier Science Publishing Co., Inc. , 1988
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
Joklik's MEM
Heart
Human
Human
Hassler, O. , Wiren, M. and Herbertsson, S.
The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase ,
Acta Pathol Microbiol Scand
 57 , 15 , 1963
Cells
Thoracic aorta
Enzymes
Collagenase : 0.2%
Medium
Phosphate buffer w/NaCl
Heart
Porcine
Porcine
Butcher, J. and Nerem, R.
Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells. ,
J Heart Valve Dis
 13 , 478 , 2004
Cells
Aortic smooth muscle and intersitial cells
Enzymes
Collagenase : 600 u/ml
Medium
PBS
Heart
Porcine
Porcine
Christ, M. , Gunther, A. , Heck, M. , Schmidt, B. , Falkenstein, E. and Wehling, M.
Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells ,
Circulation
 99 , 1485 , 1999
Cells
Coronary vascular smooth muscle (PCVSMCs)
Enzymes
Trypsin : 0.037%
Medium
HEPES
Heart
Porcine
Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise
Bowles, D. , Hu, Q. , Laughlin, M. and Sturek, M.
Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle ,
Am J Physiol
 275 (44) , H2159 , 1998
Cells
Coronary smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Krebs bicarbonate solution
Intestine
Human
Human
Trapecar, M. , Khan, S. , Roan, N. , Chen, T. , Telwatte, S. , Deswal, M. , Pao, M. , Somsouk, M. , Deeks, S. , Hunt, P. , Yukl, S. and Sanjabi, S.
An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. ,
AIDS Res Hum Retroviruses
 33 , S31-S39 , 2017
Cells
Lymphocytes
Enzymes
CLSPA : 0.05%
Deoxyribonuclease I : 0.00075%
Medium
RPMI-1640
Intestine
Human
Human
Kessler, S. , Rho, H. , West, G. , Fiocchi, C. , Drazba, J. and de la Motte, C.
Hyaluronan (HA) Deposition Precedes and Promotes Leukocyte Recruitment in Intestinal Inflammation. ,
Clin Transl Sci
 1 , 57-61 , 2008
Cells
Microvessel endothelial
Enzymes
Medium
HBSS
Intestine
Human
Human
Ebert, Ellen.
CD2 Activation of Human Lamina Propria Lymphocytes Reduces CD3 Responsiveness. ,
Immunology
 117 , 71-7 , 2006
Cells
Lamina propria lymphocytes
Enzymes
Collagenase : 20 u/ml
Medium
Medium
Intestine
Human
Human
Kanai Takanori, Totsuka Teruji, Uraushihara Koji, Makita Shin, Nakamura Tetsuya, Koganei Kazutaka, Fukushima Tsuneo, Akiba Hisaya, Yagita Hideo, Okumura Ko, Machida Utako, Iwai Hideyuki, Azuma Miyuki, Chen Lieping, Watanabe Mamoru.
Blockade of B7-H1 suppresses the development of chronic intestinal inflammation ,
J Immunol
 171 , 4156-63 , 2003
Cells
Lamina propria mononuclear cells
Enzymes
Collagenase : 0.02%
Medium
HBSS
Intestine
Human
Human
Fahlgren, A. , Hammarstrom, S. , Danielsson, A. and Hammarstrom, M.
Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. ,
Clin Exp Immunol
 131 , 90 , 2003
Cells
Intestinal epithelial
Enzymes
Collagenase Type 4 : 72.5 u/ml
Medium
HBSS
Intestine
Human
Human
Stallmach A, Schaffer F, Hoffmann S, Weber S, Muller-Molaian I, Schneider T, Kohne G, Ecker KW, Feifel G, Zeitz M.
Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis ,
Gut
 43 , 499-505 , 1998
Cells
Mucosal mononuclear cells
Enzymes
Medium
RPMI
Kidney
Human
Human
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Kidney
Human
Human, adult
Valente, M. , Henrique, R. , Costa, V. , Jeronimo, C. , Carvalho, F. , Bastos, M. , Guedes de Pinho, P. and Carvalho, M.
A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens. ,
PLoS ONE
 6 , e19337 , 2011
Cells
Renal tumor cells and proximal tubular epithelial
Enzymes
Medium
DMEM/F12
Kidney
Human
Human
Presnell, S. , Bruce, A. , Wallace, S. , Choudhury, S. , Genheimer, C. , Cox, B. , Guthrie, K. , Werdin, E. , Tatsumi-Ficht, P. , Ilagan, R. , Kelley, R. , Rivera, E. , Ludlow, J. , Wagner, B. , Jayo, M. and Bertram, T.
Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys. ,
Tiss Eng
 17 , 261 , 2011
Cells
Renal cells
Enzymes
Collagenase Type 4 : 300 u/ml
Neutral Protease : see reference
Medium
HBSS
Kidney
Human
Human, adult
Johnson, D. , Saunders, H. , Johnson, F. , Huq, S. , Field, M. and Pollock, C.
Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor ,
J Pharmacol Exp Ther
 289 , 535-42 , 1999
Cells
Renal proximal tubule and cortical fibroblasts
Enzymes
Collagenase Type 2 : 383 u/ml
Medium
DMEM/F-12
Kidney
Human
Human
McAteer, J, Kempson, S. and Evan, A
Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
 13 , 143 , 1991
Cells
Renal cortex
Enzymes
Trypsin : 0.1%
Medium
Tissue Culture Grade Water
Kidney
Human
Human
Trifillis, A. and Kahng, M.
Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells ,
In Vitro Cell Dev Biol
 26 , 441 , 1990
Cells
Papillary duct
Enzymes
Collagenase : 400 u/ml
Medium
Eagle's MEM-HEPES buffer w/L-glutamine
Kidney
Human
Human
Heieren, M. , van der Woude, F. and Balfour Jr., H.
Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
 85 , 1642 , 1988
Cells
Mesangial
Enzymes
Trypsin : 0.25%
Medium
DMEM/Ham's F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.