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Tissue References Search Tool

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Stem
Human
Human, 8-12 year
Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F.
Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche ,
Nat Med
 13 , 1219-27 , 2007
Cells
Tendon stem/progenitor
Enzymes
Medium
DMEM
Stem
Human
Human
Steigman, S. and Fauza, D.
Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1E.2 , 2007
Cells
Mesenchymal stem
Enzymes
Neutral Protease : see reference
Medium
CMF-DPBS
Stem
Human
Human, male
Lei, L. , Liao, W. , Sheng, P. , Fu, M. , He, A. and Huang, G.
Biological Character of Human Adipose-Derived Adult Stem Cells and Influence of Donor Age on Cell Replication in Culture. ,
Sci China C Life Sci
 50 , 320 , 2007
Cells
Adipose derived adult stem cells
Enzymes
Medium
DMEM
Stem
Human
Human
Kilroy, G. , Foster, S. , Wu, X. , Ruiz, J. , Sherwood, S. , Heifetz, A. , Ludlow, J. , Stricker, D. , Potiny, S. , Green, P. , Halvorsen, Y. , Cheatham, B. , Storms, R. and Gimble, J.
Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors. ,
J Cell Physiol
 212 , 702-9 , 2007
Cells
Adipose derived stem cells
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D.
Identification of Pancreatic Cancer Stem Cells. ,
Cancer Res
 67 , 1030 , 2007
Cells
Pancreatic cancer stem cells
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
medium 199
Stem
Human
Human
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei.
Isolation of a novel population of multipotent adult stem cells from human hair follicles ,
Am J Pathol
 168 , 1879-88 , 2006
Cells
Hair follicular epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Stem
Human
Human
Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho.
Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK ,
J Lipid Res
 47 , 653-64 , 2006
Cells
Mesenchymal stem
Enzymes
Medium
HBSS
Stem
Human
Human
Kern Susanne, Eichler Hermann, Stoeve Johannes, Kluter Harald, Bieback Karen.
Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue ,
Stem Cells
 24 , 1294-301 , 2006
Cells
Mesenchymal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Boquest Andrew C, Shahdadfar Aboulghassem, Brinchmann Jan E, Collas Philippe.
Isolation of stromal stem cells from human adipose tissue ,
Methods Mol Biol
 325 , 35-46 , 2006
Cells
Stromal stem cells
Enzymes
Medium
HBSS
Stem
Human
Human
Portmann-Lanz CBettina, Schoeberlein Andreina, Huber Alexander, Sager Ruth, Malek Antoine, Holzgreve Wolfgang, Surbek DanielV.
Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration ,
Am J Obstet Gynecol
 194 , 664-73 , 2006
Cells
Placental mesenchymal stem
Enzymes
Collagenase Type 2 : 270 u/ml
Neutral Protease : 2.4 u/ml
Medium
MEM
Stem
Human
Human, adult
Mitchell James B, McIntosh Kevin, Zvonic Sanjin, Garrett Sara, Floyd Z Elizabeth, Kloster Amy, Di Halvorsen Yuan, Storms Robert W, Goh Brian, Kilroy Gail, Wu Xiying, Gimble Jeffrey M.
Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers ,
Stem Cells
 24 , 376-85 , 2006
Cells
Adipose-derived adult stem
Enzymes
Medium
DMEM/F-12 Ham's
Stem
Human
Human, female
Guilak, F. , Lott, K. , Awad, H. , Cao, Q. , Hicok, K. , Fermor, B. and Gimble, J.
Clonal Analysis of the Differentiation Potential of Human Adipose-Derived Adult Stem Cells. ,
J Cell Physiol
 206 , 229 , 2006
Cells
Adipose derived adult stem cells
Enzymes
Medium
DMEM/F-12
Stem
Human
Human
Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E.
Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture ,
Mol Biol Cell
 16 , 1131-41 , 2005
Cells
Adipocytes, stromal vascular
Enzymes
Collagenase : 0.2%
Medium
HBSS
Stem
Human
Human
Devireddy, R. , Thirumala, S. and Gimble, J.
Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress. ,
J Biomech Eng
 127 , 1081 , 2005
Cells
Adult stem cells
Enzymes
Medium
PBS
Stem
Human
Human
Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A.
Improvement of postnatal neovascularization by human adipose tissue-derived stem cells ,
Circulation
 110 , 349-55 , 2004
Cells
Stromal vascular, adipocytes, stem
Enzymes
Collagenase : 300 u/ml
Medium
PBS
Stem
Human
Human, neonatal
Li, A. , Pouliot, N. , Redvers, R. and Kaur, P.
Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny ,
J Clin Invest
 113 , 390-400 , 2004
Cells
Keratinocytes
Enzymes
Medium
DMEM
Stem
Human
Human, female
Aust, L. , Devlin, B. , Foster, S. , Halvorsen, Y. , Hicok, K. , du Laney, T. , Sen, A. , Willingmyre, G. and Gimble, J.
Yield of Human Adipose-Derived Adult Stem Cells from Liposuction Aspirates. ,
Cytometry
 6 , 7-14 , 2004
Cells
Adipose derived adult stem cells
Enzymes
Medium
DMEM-Ham's F-12
Stem
Human
Human
Covas DT, Siufi JL C, Silva AR L, Orellana MD.
Isolation and culture of umbilical vein mesenchymal stem cells ,
Braz J Med Biol Res
 36 , 1179-83 , 2003
Cells
Umbilical vein mesenchymal stem cells
Enzymes
Medium
PBS
Stem
Human
Human, adult, male
Papini S, Cecchetti D, Campani D, Fitzgerald W, Grivel J, Chen S, Margolis L, Revoltella R.
Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture ,
Stem Cells
 21 , 481 , 2003
Cells
Human epidermal keratinocyte stem cells
Enzymes
Medium
DMEM
Stem
Human
Human, 7-8 yr
Miura, M. , Gronthos, S, Zhao, M, Lu, B, Fisher, L, Robey, P. and Shi, S.
SHED: Stem Cells from Human Exfoliated Deciduous Teeth ,
Proc Natl Acad Sci U S A
 100 , 5807 , 2003
Cells
Stem cells Human exfoliated deciduous teeth
Enzymes
Medium
see reference
Stem
Human
Human, fetal
Malhi, H. , Irani, A. , Gagandeep, S. and Gupta, S.
Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes. ,
J Cell Sci
 115 , 2679 , 2002
Cells
Epithelial progenitor
Enzymes
Collagenase : 0.03%
Medium
DMEM
Stem
Human
Human
Zuk, P. , Zhu, M. , Ashjian, P. , De Ugarte, D. , Huang, J. , Mizuno, H. , Alfonso, Z. , Fraser, J. , Benhaim, P. and Hedrick, M.
Human adipose tissue is a source of multipotent stem cells ,
Mol Biol Cell
 13 , 4279-95 , 2002
Cells
Processed lipoaspirate cells
Enzymes
Medium
PBS
Stem
Human
Human
Zhang, S. , Wernig, M. , Duncan, I. , Brustle, O. and Thomson, J.
In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells ,
Nat Biotechnol
 19 , 1129 , 2001
Cells
Stem, embryonic
Enzymes
Neutral Protease : 0.01% - 0.02%
Medium
DMEM
Stem
Human
Human, adult
Kambe, N, Kambe, M, Kochan, J. and Schwartz ,L.
Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes ,
Blood
 97 , 2045 , 2001
Cells
Human skin mast cells
Enzymes
Medium
HBSS
Stem
Human
Human
Uchida, N. , Buck, D. , He, D. , Reitsma, M. , Masek, M. , Phan, T. , Tsukamoto, A. , Gage, F. and Weissman, I.
Direct Isolation of Human Central Nervous System Stem Cells. ,
Proc Natl Acad Sci U S A
 97 , 14720 , 2000
Cells
Central nervous system stem
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
HBSS
Stem
Human
Human
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM.
Embryonic stem cell lines derived from human blastocysts ,
Science
 282 , 1145-7 , 1998
Cells
Embryonic stem
Enzymes
Medium
DMEM
Stem
Human
Human
Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio.
Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages ,
Lancet
 364 , 1872-83
Cells
Muscle-derived stem cells
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Thymus
Human
Human
Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K.
Human thymus contains 2 distinct dendritic cell populations ,
Blood
 97 , 1733-41 , 2001
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Thyroid/Parathyroid
Human

Human

Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y.
Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022
Cells
Thyroid normal and tumor
Enzymes
Trypsin : 0.125%
Collagenase Type 4 : 300 u/ml
Medium
RPMI-1640
Thyroid/Parathyroid
Human
Human
Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.
Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
 284 , E168 , 2003
Cells
Endothelial
Enzymes
Medium
DMEM
Thyroid/Parathyroid
Human
Human
Gianoukakis, A. , Cao, H. , Jennings, T. and Smith, T.
Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells. ,
Am J Physiol Cell Physiol
 280 , C701 , 2001
Cells
Thyrocytes
Enzymes
Collagenase Type 1 : 130 u/ml
Neutral Protease : 0.5 u/ml
Medium
HBSS
Thyroid/Parathyroid
Human
Human
Howie, A. , Walker, S. , Akesson, B. , Arthur, J. and Beckett, G.
Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis. ,
Biochem J
 308 ( Pt 3) , 713 , 1995
Cells
Thyrocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.1%
Medium
EBSS
Thyroid/Parathyroid
Human
Human
Miller, R. , Hiraoka, T. , Nakamura, N. , Tenou, H. , Kopecky, K. , Jones, M. and Gould, M.
In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology. ,
J Radiat Res
 26 , 269 , 1985
Cells
Thyroid
Enzymes
Collagenase : 300 u/ml
Medium
Ham's F-12/MEM
Thyroid/Parathyroid
Ovine
Sheep
Kerkof, P.
Preparation of Primary Cultures of Ovine Thyroid Gland Cells ,
J Tiss Cul Meth
 7 , 23 , 1982
Cells
Thyroid
Enzymes
Collagenase : 0.2%
Medium
Puck's Saline F
Tonsil
Human
Human
Muto, M. , Manfroi, B. , Suzuki, H. , Joh, K. , Nagai, M. , Wakai, S. and Righini, C. et al.
Toll-Like Receptor 9 Stimulation Induces Aberrant Expression of a Proliferation-Inducing Ligand by Tonsillar Germinal Center B Cells in IgA Nephropathy. ,
J Am Soc Nephrol
 28 , 1227-1238 , 2017
Cells
Tonsillar
Enzymes
Medium
see reference
Tonsil
Human
Human
Schmidt, S. , Schenkova, K. , Adam, T. , Erikson, E. , Lehmann-Koch, J. , Sertel, S. , Verhasselt, B. , Fackler, O. , Lasitschka, F. and Keppler, O.
SAMHD1's Protein Expression Profile in Humans. ,
J Leukoc Biol
 98 , 5-14 , 2015
Cells
Tonsillar
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.