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Tissue References Search Tool

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Adrenal
Human
Human
Santana, M. , Chung, K. , Vukicevic, V. , Rosmaninho-Salgado, J. , Kanczkowski, W. , Cortez, V. , Hackmann, K. , Bastos, C. , Mota, A. , Schrock, E. , Bornstein, S. , Cavadas, C. and Ehrhart-Bornstein, M.
Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla. ,
Stem Cells Transl Med
 1 , 783 , 2012
Cells
Adrenal medulla progenitor
Enzymes
Collagenase : 0.2%
Medium
DMEM/F-12
Adrenal
Human
Human
Jeon, Y. , Baek, W. , Chung, S. , Shin, N. , Kim, H. and Lee, S.
Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model. ,
Korean J Anesthesiol
 60 , 357 , 2011
Cells
Chromaffin cells
Enzymes
Collagenase : 0.2%
Medium
Locke's solution
Adrenal
Human
Human
Caroccia, B. , Fassina, A. , Seccia, T. , Recarti, C. , Petrelli, L. , Belloni, A. , Pelizzo, M. and Rossi, G.
Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method. ,
Endocrinology
 151 , 1375 , 2010
Cells
Adrenocortical
Enzymes
Medium
Krebs Ringer
Adrenal
Human
Human, adult
Tischler, A. , DeLellis, R. , Bailes, B. , Nunnemacher, G.,Carabba, V. and Wolfe, H.
Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells ,
Lab Invest
 43 , 399 , 1980
Cells
Chromaffin
Enzymes
Trypsin : 0.25%
Medium
Eagle's MEM
Adrenal
Human
Human, adult and child
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Human

Human

Klauzen, P. , Semenova, D. , Kostina, D. , Uspenskiy, V. and Malashicheva, A.
Purinergic Signaling in Pathologic Osteogenic Differentiation of Aortic Valve Interstitial Cells from Patients with Aortic Valve Calcification. , Biomedicines Vol. 11 , 2023
Cells
Valve interstitial and endothelial
HUVEC
Osteoblast
Enzymes
Medium
ECM
Endothelial
Human
Human
Ganguly, A. , Zhang, H. , Sharma, R. , Parsons, S. and Patel, K.
Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions. ,
J Vis Exp
 66 , e4032 , 2012
Cells
Vascular endothelial
Enzymes
Medium
HBSS
Endothelial
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Endothelial
Human
Human
Moss, S. , Bates, M. , Parrino, P. and Woods, TC.
Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue. ,
Ochsner J
 7 , 133 , 2007
Cells
Endothelial and vascular smooth muscle
Enzymes
Medium
HBSS
Endothelial
Human
Human
Davis, J. , Crampton, S. and Hughes. C.
Isolation of Human Umbilical Vein Endothelial Cells (HUVEC). ,
J Vis Exp
 3 , 183 , 2007
Cells
HUVEC
Enzymes
Medium
HBSS
Endothelial
Human
Human
Silva AP, Kaufmann JE, Vivancos C, Fakan S, Cavadas C, Shaw P, Brunner HR, Vischer U. and Grouzmann E.
Neuropeptide Y expression, localization and cellular transducing effects in HUVEC ,
Biol Cell
 97(6) , 457 , 2005
Cells
HUVEC
Enzymes
Medium
RPMI 1640
Endothelial
Human
Human
Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.
Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
 284 , E168 , 2003
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Human
Human
Rafiee, P. , Ogawa, H. , Heidemann, J. , Li, M. , Aslam, M. , Lamirand, T. , Fisher, P. , Graewin, S. , Dwinell, M. , Johnson, C. , Shaker, R. and Binion, D.
Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation. ,
Am J Physiol Gastrointest Liver Physiol
 285 , G1277 , 2003
Cells
Esophageal microvascular endothelial
Enzymes
Medium
MCDB-131
Endothelial
Human
Human
Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM. and Kirkpatrick CJ.
Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro ,
Exp Mol Pathol
 72 , 221 , 2002
Cells
Pulmonary vascular endothelial cells
Enzymes
Neutral Protease : 1.18 u/ml
Elastase : 10 u/ml
Medium
M199
Endothelial
Human
Human
Takano Manabu, Meneshian Avedis, Sheikh Emran, Yamakawa Yasuhiko, Wilkins Kirsten Bass, Hopkins Elise A, Bulkley Gregory B.
Rapid upregulation of endothelial P-selectin expression via reactive oxygen species generation ,
Am J Physiol Heart Circ Physiol
 283 , H2054-61 , 2002
Cells
HUVEC
Enzymes
Medium
PBS
Endothelial
Human
Human
Wang D. , Lehman R. , Donner D. , Matli M. , Warren R. and Welton M.
Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells ,
Am J Physiol/Gastro
 282 , G1088 , 2002
Cells
Colonic endothelial cells
Enzymes
Medium
HBSS/5%FBS
Endothelial
Human
Human
Goolcharran, C. , Cleland, J. , Keck, R. , Jones, A. and Borchardt, R.
Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides ,
AAPS PharmSci
 2 (1) , 5 , 2000
Cells
Endothelial
Enzymes
Trypsin : 2%
Medium
PBS
Endothelial
Human
Human
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY. and Koh GY.
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells ,
Circulation
 101(19) , 2317 , 2000
Cells
HUVEC, porcine pulmonary arterial endothelial cells
Enzymes
Medium
DMEM
Endothelial
Human
Human
Schonbeck U, Sukhova GK, Graber P, Coulter S, Libby P.
Augmented expression of cyclooxygenase-2 in human atherosclerotic lesions ,
Am J Pathol
 155 , 1281-91 , 1999
Cells
Vascular endothelial cells
Enzymes
Collagenase : 0.1%
Medium
DMEM
Endothelial
Human
Human
Sanyal AJ. and Mirshahi F.
A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts ,
Lab Invest
 78(11) , 1469 , 1998
Cells
Hepatic endothelial cells
Enzymes
Collagenase : 0.2%
Medium
DMEM
Endothelial
Human
Human
Wojta J, Gallicchio M, Zoellner H, Filonzi E, Hamilton J, McGrath K.
Interleukin-4 stimulates expression of urokinase-type-plasminogen activator in cultured human foreskin microvascular endothelial cells ,
Blood
 81 , 3285-92 , 1993
Cells
Foreskin microvascular endothelial
Enzymes
Trypsin : 0.3%
Medium
HBSS
Endothelial
Human
Human
Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ.
Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis ,
Am J Physiol
 262 , H1088-95 , 1992
Cells
Vascular endothelial
Enzymes
Trypsin : 0.25%
Medium
M-199
Endothelial
Human
Human
Lee, K, Lawley, T, Xu, Y. and Swerlick, R.
VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells ,
J Invest Dermatol
 98 , 79 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.3.%
Medium
HBSS
(see reference)
Endothelial
Human
Human
Grant, D.S. , Lelkes, P.I. , Fukuda, K. and Kleinman, H.K.
Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro ,
In Vitro Cell Dev Biol
 27 , 327 , 1991
Cells
Umbilical vein
HUVEC
Enzymes
Collagenase : 0.1%
Medium
Cord Buffer
(See Reference)
Endothelial
Human
Human
Whitehead, R. and Eeden, P.
A Method For the Prolonged Culture of Colonic Epithelial Cells ,
J Tiss Cul Meth
 13 , 103 , 1991
Cells
Crypt cells
Enzymes
Collagenase : 125 u/ml
Medium
RMPI 1640
Endothelial
Human
Human
Fischer, E. , Stingl, A. and Kirkpatrick, C.
Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids ,
J Immunol Methods
 128 , 235 , 1990
Cells
Endothelial
Enzymes
Medium
Medium 199
Endothelial
Human
Human
Muller WA, Ratti CM, McDonnell SL, Cohn ZA.
A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions ,
J Exp Med
 170 , 399-414 , 1989
Cells
Human umbilical vein endothelial cells
Enzymes
Collagenase Type 2 : 75 u/ml
Medium
M199
Endothelial
Human
Human
Kubota, Y. , Kleinman, H. , Martin, G. and Lawley, T.
Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures ,
J Cell Biol
 107 , 1589 , 1988
Cells
Endothelial/HUVEC
Foreskin & umbilical cord
Enzymes
Trypsin : 0.3%
Medium
HBSS/PBS, Medium 199
(see reference)
Endothelial
Human
Human
Hoshi, H. , Kan, M. , Chen, J. and McKeehan, W.
Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells ,
In Vitro Cell Dev Biol
 24 (4) , 309 , 1988
Cells
Umbilical cord
Smooth muscle
Enzymes
Collagenase : 0.1%
Medium
HEPES
Endothelial
Human
Human
Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R.
Cutlured Human and Canine Endothelial Cells from Brain Microvessels ,
Brain Res Bull
 21 , 785 , 1988
Cells
Microvessels
Enzymes
Medium
DMEM
Endothelial
Human
Human
Hoshi, H. and McKeehan, W.
Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium ,
In Vitro Cell Dev Biol
 22 (1) , 51 , 1986
Cells
Endothelial
Enzymes
Collagenase : 0.1%
Medium
HEPES
Endothelial
Human
Human
Sharefkin, J.B. , Fairchild, K.D. , Albus, R.A. , Cruess, D.F. and Rich, N.M.
The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques ,
J Surg Res
 41 , 463 , 1986
Cells
Endothelial
Saphenous vein
Enzymes
Medium
PBS, CMF
Endothelial
Human
Human
Marks, R.M. , Czerniecki, M. and Penny, R.
Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture ,
In Vitro Cell Dev Biol
 21 , 627 , 1985
Cells
Endothelial
Dermal
Enzymes
Trypsin : 0.3%
Medium
PBS
Endothelial
Human
Human
Gordon, P. , Sussman, I. and Hatcher, V.
Long-Term Culture of Human Endothelial Cells ,
In Vitro
 19 , 661 , 1983
Cells
Fibroblasts
Foreskin
Enzymes
Hyaluronidase : 0.10%
Medium
DMEM
Endothelial
Human
Human
Glassberg, M. , Bern, M. , Coughlin, S. , Haudenschild, C. , Hoyer, L. and Antoniades, H.
Cultured Endothelial Cells Derived from the Human Iliac Arteries ,
In Vitro
 18 , 859 , 1982
Cells
Iliac arteries
Enzymes
Collagenase : 0.25%
Medium
PBS w/Ca++, Mg++, & BSA
(see reference)
Endothelial
Human
Human, adult
Ashida, E. , Johnson, A. and Lipsky, P.
Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro ,
J Clin Invest
 67 , 1490 , 1981
Cells
Peripheral blood mononuclear
Monocytes
T cells
Endothelial
Enzymes
Collagenase : 0.25%
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.