Tissue References Search Tool

Human

Santana, M. , Chung, K. , Vukicevic, V. , Rosmaninho-Salgado, J. , Kanczkowski, W. , Cortez, V. , Hackmann, K. , Bastos, C. , Mota, A. , Schrock, E. , Bornstein, S. , Cavadas, C. and Ehrhart-Bornstein, M.

Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla. ,
Stem Cells Transl Med
1 , 783 , 2012

Cells

Adrenal medulla progenitor

Enzymes

Collagenase : 0.2%

Medium

DMEM/F-12

Adrenal

Human

Jeon, Y. , Baek, W. , Chung, S. , Shin, N. , Kim, H. and Lee, S.

Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model. ,
Korean J Anesthesiol
60 , 357 , 2011

Cells

Chromaffin cells

Enzymes

Collagenase : 0.2%

Medium

Locke's solution

Adrenal

Human

Caroccia, B. , Fassina, A. , Seccia, T. , Recarti, C. , Petrelli, L. , Belloni, A. , Pelizzo, M. and Rossi, G.

Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method. ,
Endocrinology
151 , 1375 , 2010

Cells

Adrenocortical

Enzymes

Collagenase Type 1 : 0.2%

Deoxyribonuclease I : 0.01%

Medium

Krebs Ringer

Adrenal

Human

Human, adult

Tischler, A. , DeLellis, R. , Bailes, B. , Nunnemacher, G.,Carabba, V. and Wolfe, H.

Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells ,
Lab Invest
43 , 399 , 1980

Cells

Chromaffin

Enzymes

Trypsin : 0.25%

Medium

Eagle's MEM

Adrenal

Human

Human, adult and child

Folkman, J. , Haudenschild, C. C. and Zetter, B. R.

Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
76 , 5217 , 1979

Cells

Foreskin

Enzymes

Collagenase : 0.5%

Medium

Dulbecco's MEM w/10% calf serum

Heart

Human

Holt-Casper, D. , Theisen, J. , Moreno, A. , Warren, M. , Silva, F. , Grainger, D. , Bull, D. and Patel, A.

Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds. ,
J Transl Med
13 , 194 , 2015

Cells

Cardiac

Enzymes

Collagenase : 0.045%

Pancreatin : 0.1%

Medium

DPBS

Heart

Human

Sharma, S. , Mishra, R. , Simpson, D. , Wehman, B. , Colletti, E. , Deshmukh, S. , Datla, S. , Balachandran, K. , Guo, Y. , Chen, L. , Siddiqui, O. , Kaushal, S. and Kaushal, S.

Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome. ,
Stem Cells
33 , 1213-29 , 2015

Cells

Cardiospheres

Enzymes

Collagenase Type 2 : 0.1%

Neutral Protease : 0.08%

Trypsin : 0.05%

Medium

Iscove modified DMEM

Heart

Human

Avolio, E. , Gianfranceschi, G. , Cesselli, D. , Caragnano, A. , Athanasakis, E. , Katare, R. , Meloni, M. , Palma, A. , Barchiesi, A. , Vascotto, C. , Toffoletto, B. , Mazzega, E. , Finato, N. , Aresu, G. and Livi, U.

Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. ,
Stem Cells
32 , 2373 , 2014

Cells

Cardiac stem

Enzymes

Collagenase Type 2 : 0.25%

Medium

Joklik modified Eagle’s

Heart

Human

Voigt, N. , Zhou, X. and Dobrev, D.

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. ,
J Vis Exp
, e50235 , 2013

Cells

Atrial myocytes

Enzymes

Collagenase Type 1 : 286 u/ml

Protease : 5 u/ml

Medium

see references

Heart

Human

Vukusic, K. , Jonsson, M. , Brantsing, C. , Dellgren, G. , Jeppsson, A. , Lindahl, A. and Asp, J.

High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis. ,
Biomed Res Int
2013 , 2013

Cells

Cardiac

Enzymes

Collagenase Type 2 : 0.08%

Medium

DMEM

Heart

Human

Anderson, E. , Rodriguez, E. , Anderson, C. , Thayne, K. , Chitwood, W. and Kypson, A.

Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways. ,
Am J Physiol Heart Circ Physiol
300 , H118 , 2011

Cells

Myofibers

Enzymes

Collagenase Type 1 : 0.3%

Medium

see reference

Heart

Human

Voigt, N. , Makary, S. , Nattel, S. and Dobrev, D.

Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart. ,
Methods Enzymol
484 , 653 , 2010

Cells

Atrial cardiomyocytes

Enzymes

Collagenase Type 1 : 286 u/ml

Protease : 5 u/ml

Medium

see reference

Heart

Human

Jensen, B. , Swigart, P. , Laden, M. , DeMarco, T. , Hoopes, C. and Simpson, P.

The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries. ,
J Am Coll Cardiol
54 , 1137 , 2009

Cells

Coronary artery smooth muscle

Enzymes

Collagenase Type 2 : 0.1%

Elastase : 0.05%

Medium

DMEM

Heart

Human

Mathew, S. , Tustison, K. , Sugatani, T. , Chaudhary, L. , Rifas, L. and Hruska, K.

The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD. ,
J Am Soc Nephrol
19 , 1092 , 2008

Cells

Smooth muscle aortic

Enzymes

Collagenase Type 1 : 0.2%

Collagenase Type 2 : 0.1%

Elastase : 0.025%

Medium

DMEM

Heart

Human

Human (n=16, age: 60 &plusmin;3 years)

Mukerjee, R. , Multani, M. , Sample, J. , Dowdy, K. , Zellner, J. , Hoover, D. and Spinale, F.

Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response ,
J Cardiovasc Pharmacol
7 (4) , 235 , 2002

Cells

Myocardial

Enzymes

Hyaluronidase : 0.05%

Medium

Medium 199

Heart

Human

Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N.

Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes ,
Am J Physiol Heart Circ Physiol
283 , H990-5 , 2002

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 0.05%

Collagenase Type 1 : 0.025%

Protease XIV : 0.013%

Medium

HEPES solution

Heart

Human

Shirai, T. , Rao, V. , Weisel, R. , Ikonomidis, J. , Li, R. , Tumiati, L. , Merante, F. and Mickle, D.

Preconditioning Human Cardiomyocytes and Endothelial Cells. ,
J Thorac Cardiovasc Surg
115 , 210-9 , 1998

Cells

Cardiomyocytes and endothelial

Enzymes

Trypsin : 0.2%

Collagenase : 0.1%

Medium

PBS

Heart

Human

Hoppe, U. , Jansen, E. , Sudkamp, M. and Beukelmann, D.

Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts ,
Circulation
97 , 55 , 1998

Cells

Heart

Myocytes

Enzymes

Collagenase Type 2 : 200 u/ml

Medium

Tyrode's solution

Heart

Human

Van Wagoner, D. , Pond, A. , McCarthy, P. , Trimmer, J. and Nerbonne, J.

Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation. ,
Circ Res
80 , 772-81 , 1997

Cells

Atrial myocytes

Enzymes

Collagenase Type 2 : 0.1%

Protease Type XIV : 0.04%

Medium

see reference

Heart

Human

Human, fetal, 14.5 week gestation

Goldman, B. and Wurzel, J.

Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes ,
In Vitro Cell Dev Biol
28 , 109 , 1992

Cells

Myocytes

Enzymes

Hyaluronidase : 0.1%

Medium

HBSS with Calcium

Heart

Human

Human, both sexes, 18-81 years

Smith, D. , Glover, J. , Townsend, L. and Maupin, E.

A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor ,
In Vitro Cell Dev Biol
27 , 914 , 1991

Cells

Myocardial

Atrial

Enzymes

Collagenase : 0.14%

Medium

HBSS, CMF

Heart

Human

Human, adult

Bugaisky, L.B.

Biology of Isolated Adult Cardiac Myocytes , Isolation and Culture of Human Adult Cardiac Myocytes , (Clark,Decker,Borg,eds) , Elsevier Science Publishing Co., Inc. , 1988

Cells

Myocytes

Enzymes

Trypsin : 0.25%

Medium

Joklik's MEM

Heart

Human

Hassler, O. , Wiren, M. and Herbertsson, S.

The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase ,
Acta Pathol Microbiol Scand
57 , 15 , 1963

Cells

Thoracic aorta