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Tissue References Search Tool

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Bone
Human

Human

Klauzen, P. , Semenova, D. , Kostina, D. , Uspenskiy, V. and Malashicheva, A.
Purinergic Signaling in Pathologic Osteogenic Differentiation of Aortic Valve Interstitial Cells from Patients with Aortic Valve Calcification. , Biomedicines Vol. 11 , 2023
Cells
Valve interstitial and endothelial
HUVEC
Osteoblast
Enzymes
Medium
ECM
Bone
Human
Human
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Human
Human
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Human
Human
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Human
Human, 22-73 year
Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ.
Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute ,
Cells Tissues Organs
 179 , 158-69 , 2005
Cells
Osteoblasts
Enzymes
Trypsin : 0.5%
Medium
Basal Medium
Bone
Human
Human, 60+ year
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Human
Human
Meikle, M. , Boyd, S. , Hembry, R. , Compston, J. , Croucher, P. and Reynolds, J.
Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines ,
J Cell Sci
 103 , 1093 , 1992
Cells
Osteoblasts
Enzymes
Trypsin : 0.1%
Medium
DMEM
Bone
Human
Human
Fedarko, N.S. , Termine, J.D. , Young, M.F. and Robey, P.G.
Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro ,
J Biol Chem
 265 , 12200 , 1990
Cells
Osteoblasts
Enzymes
Collagenase Type 4 : 250 u/ml
Medium
DMEM
Heart
Human
Human
Holt-Casper, D. , Theisen, J. , Moreno, A. , Warren, M. , Silva, F. , Grainger, D. , Bull, D. and Patel, A.
Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds. ,
J Transl Med
 13 , 194 , 2015
Cells
Cardiac
Enzymes
Collagenase : 0.045%
Pancreatin : 0.1%
Medium
DPBS
Heart
Human
Human
Sharma, S. , Mishra, R. , Simpson, D. , Wehman, B. , Colletti, E. , Deshmukh, S. , Datla, S. , Balachandran, K. , Guo, Y. , Chen, L. , Siddiqui, O. , Kaushal, S. and Kaushal, S.
Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome. ,
Stem Cells
 33 , 1213-29 , 2015
Cells
Cardiospheres
Enzymes
Medium
Iscove modified DMEM
Heart
Human
Human
Avolio, E. , Gianfranceschi, G. , Cesselli, D. , Caragnano, A. , Athanasakis, E. , Katare, R. , Meloni, M. , Palma, A. , Barchiesi, A. , Vascotto, C. , Toffoletto, B. , Mazzega, E. , Finato, N. , Aresu, G. and Livi, U.
Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. ,
Stem Cells
 32 , 2373 , 2014
Cells
Cardiac stem
Enzymes
Medium
Joklik modified Eagle’s
Heart
Human
Human
Voigt, N. , Zhou, X. and Dobrev, D.
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. ,
J Vis Exp
 , e50235 , 2013
Cells
Atrial myocytes
Enzymes
Collagenase Type 1 : 286 u/ml
Protease : 5 u/ml
Medium
see references
Heart
Human
Human
Vukusic, K. , Jonsson, M. , Brantsing, C. , Dellgren, G. , Jeppsson, A. , Lindahl, A. and Asp, J.
High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis. ,
Biomed Res Int
 2013 , 2013
Cells
Cardiac
Enzymes
Medium
DMEM
Heart
Human
Human
Anderson, E. , Rodriguez, E. , Anderson, C. , Thayne, K. , Chitwood, W. and Kypson, A.
Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways. ,
Am J Physiol Heart Circ Physiol
 300 , H118 , 2011
Cells
Myofibers
Enzymes
Medium
see reference
Heart
Human
Human
Voigt, N. , Makary, S. , Nattel, S. and Dobrev, D.
Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart. ,
Methods Enzymol
 484 , 653 , 2010
Cells
Atrial cardiomyocytes
Enzymes
Collagenase Type 1 : 286 u/ml
Protease : 5 u/ml
Medium
see reference
Heart
Human
Human
Jensen, B. , Swigart, P. , Laden, M. , DeMarco, T. , Hoopes, C. and Simpson, P.
The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries. ,
J Am Coll Cardiol
 54 , 1137 , 2009
Cells
Coronary artery smooth muscle
Enzymes
Elastase : 0.05%
Medium
DMEM
Heart
Human
Human
Mathew, S. , Tustison, K. , Sugatani, T. , Chaudhary, L. , Rifas, L. and Hruska, K.
The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD. ,
J Am Soc Nephrol
 19 , 1092 , 2008
Cells
Smooth muscle aortic
Enzymes
Medium
DMEM
Heart
Human
Human (n=16, age: 60 &plusmin;3 years)
Mukerjee, R. , Multani, M. , Sample, J. , Dowdy, K. , Zellner, J. , Hoover, D. and Spinale, F.
Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response ,
J Cardiovasc Pharmacol
 7 (4) , 235 , 2002
Cells
Myocardial
Enzymes
Hyaluronidase : 0.05%
Medium
Medium 199
Heart
Human
Human
Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N.
Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 283 , H990-5 , 2002
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.013%
Medium
HEPES solution
Heart
Human
Human
Shirai, T. , Rao, V. , Weisel, R. , Ikonomidis, J. , Li, R. , Tumiati, L. , Merante, F. and Mickle, D.
Preconditioning Human Cardiomyocytes and Endothelial Cells. ,
J Thorac Cardiovasc Surg
 115 , 210-9 , 1998
Cells
Cardiomyocytes and endothelial
Enzymes
Trypsin : 0.2%
Collagenase : 0.1%
Medium
PBS
Heart
Human
Human
Hoppe, U. , Jansen, E. , Sudkamp, M. and Beukelmann, D.
Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts ,
Circulation
 97 , 55 , 1998
Cells
Heart
Myocytes
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
Tyrode's solution
Heart
Human
Human
Van Wagoner, D. , Pond, A. , McCarthy, P. , Trimmer, J. and Nerbonne, J.
Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation. ,
Circ Res
 80 , 772-81 , 1997
Cells
Atrial myocytes
Enzymes
Protease Type XIV : 0.04%
Medium
see reference
Heart
Human
Human, fetal, 14.5 week gestation
Goldman, B. and Wurzel, J.
Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes ,
In Vitro Cell Dev Biol
 28 , 109 , 1992
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS with Calcium
Heart
Human
Human, both sexes, 18-81 years
Smith, D. , Glover, J. , Townsend, L. and Maupin, E.
A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor ,
In Vitro Cell Dev Biol
 27 , 914 , 1991
Cells
Myocardial
Atrial
Enzymes
Collagenase : 0.14%
Medium
HBSS, CMF
Heart
Human
Human, adult
Bugaisky, L.B.
Biology of Isolated Adult Cardiac Myocytes , Isolation and Culture of Human Adult Cardiac Myocytes , (Clark,Decker,Borg,eds) , Elsevier Science Publishing Co., Inc. , 1988
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
Joklik's MEM
Heart
Human
Human
Hassler, O. , Wiren, M. and Herbertsson, S.
The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase ,
Acta Pathol Microbiol Scand
 57 , 15 , 1963
Cells
Thoracic aorta
Enzymes
Collagenase : 0.2%
Medium
Phosphate buffer w/NaCl

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.