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Tissue References Search Tool

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Bone
Human

Human

Klauzen, P. , Semenova, D. , Kostina, D. , Uspenskiy, V. and Malashicheva, A.
Purinergic Signaling in Pathologic Osteogenic Differentiation of Aortic Valve Interstitial Cells from Patients with Aortic Valve Calcification. , Biomedicines Vol. 11 , 2023
Cells
Valve interstitial and endothelial
HUVEC
Osteoblast
Enzymes
Medium
ECM
Bone
Human
Human
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Human
Human
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Human
Human
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Human
Human, 22-73 year
Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ.
Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute ,
Cells Tissues Organs
 179 , 158-69 , 2005
Cells
Osteoblasts
Enzymes
Trypsin : 0.5%
Medium
Basal Medium
Bone
Human
Human, 60+ year
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Human
Human
Meikle, M. , Boyd, S. , Hembry, R. , Compston, J. , Croucher, P. and Reynolds, J.
Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines ,
J Cell Sci
 103 , 1093 , 1992
Cells
Osteoblasts
Enzymes
Trypsin : 0.1%
Medium
DMEM
Bone
Human
Human
Fedarko, N.S. , Termine, J.D. , Young, M.F. and Robey, P.G.
Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro ,
J Biol Chem
 265 , 12200 , 1990
Cells
Osteoblasts
Enzymes
Collagenase Type 4 : 250 u/ml
Medium
DMEM
Pancreas
Human
Human
Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L.
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. ,
Cell Rep
 22 , 2667-2676 , 2018
Cells
Islets
Enzymes
Collagenase Type 1 : 1,600u/isolation
Neutral Protease : 200 u/isolation
Deoxyribonuclease I : 12,000 u/isolation
Medium
RPMI
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Human
Human
Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D.
Identification of Pancreatic Cancer Stem Cells. ,
Cancer Res
 67 , 1030 , 2007
Cells
Pancreatic cancer stem cells
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
medium 199
Pancreas
Human
Human, 25-55 yrs old
(also porcine)
Contractor, H. , Johnson, P. , Chadwick, D. , Robertson, G. and London, N.
The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas ,
Cell Transplant
 4 , 615 , 1995
Cells
Islets
Enzymes
Collagenase : 0.4%
Medium
HBSS
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Pancreas
Human
Human, adult organ donors
Warnock, G. , Rajotte, R. , Evans, M. , Ellis, D. , DeGroot, T. and Dawidson, I.
Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas ,
Transplant Proc
 XIX (4) , 3466 , 1987
Cells
Islets
Enzymes
Collagenase : 0.6%
Medium
Eurocollins solution
Pancreas
Human
Human
Izumi, R. , Konishi, K. , Ueno, K. , Shimizu, K. , Hirosawa, H. , Takahashi, N. and Miyazaki, I.
Isolation of Human Pancreatic Islets from Cryopreserved Pancreas ,
Transplant Proc
 XVII , 383 , 1985
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human
Gray, D. , McShane, P. , Grant, A. and Morris, P.
A Method for Isolation of Islets of Langerhans from the Human Pancreas ,
Diabetes
 33 , 1055 , 1984
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human, infant, age 1 day-1 year
Sutherland, D. , Matas, A. , Steffes, M. and Najarian, J.
Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation ,
Diabetes
 25 (12) , 1123 , 1976
Cells
Islets
Enzymes
Collagenase : 170-210 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.