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Tissue References Search Tool

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Cartilage
Human
Human
Andrews, S. , Kunze, M. , Mulet-Sierra, A. , Williams, L. , Ansari, K. , Osswald, M. and Adesida, A.
Strategies to Mitigate Variability in Engineering Human Nasal Cartilage. ,
Sci Rep
 7 , 6490 , 2017
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human
Muraglia, A. , Nguyen, V. , Nardini, M. , Mogni, M. , Coviello, D. , Dozin, B. , Strada, P. , Baldelli, I. , Formica, M. , Cancedda, R. and Mastrogiacomo, M.
Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support. ,
Front Bioeng Biotechnol
 5 , 66 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Hyaluronidase : 0.1%
Collagenase Type 2 : 1000 u/ml
Collagenase Type 1 : 400 u/ml
Medium
Ham's F12
Cartilage
Human
Human
Tamai, M. , Nagasao, T. , Yanaga, H. , Hamamoto, Y. , Kogure, T. and Tanaka, Y.
Correction of Secondary Deformity after Nuss Procedure for Pectus Excavatum by Means of Cultured autologous Cartilage Cell Injection. ,
Int J Surg Case Rep
 15 , 70-3 , 2015
Cells
Chondrocytes
Enzymes
Collagenase : 0.3%
Medium
DMEM
Cartilage
Human
Human
Capsoni, F. , Ongari, A. , Lonati, C. , Accetta, R. , Gatti, S. and Catania, A.
Alpha-Melanocyte-Stimulating-Hormone (a-MSH) Modulates Human Chondrocyte Activation Induced by Proinflammatory Cytokines. ,
BMC Musculoskelet Disord
 16 , 154 , 2015
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human, 52-82 years
Pallu, S. , Francin, P. , Guillaume, C. , Gegout-Pottie, P. , Netter, P. , Mainard, D. , Terlain, B. and Presle, N.
Obesity Affects the Chondrocyte Responsiveness to Leptin in Patients with Osteoarthritis. ,
Arthritis Res Ther
 12 , R112 , 2010
Cells
Chondrocytes
Enzymes
Pronase : 0.15%
Medium
DMEM/F12
Cartilage
Human
Human
Kim Wan-Uk, Kwok Seung-Ki, Hong Kyung-Hee, Yoo Seung-Ah, Kong Jin-Sun, Choe Jongseon, Cho Chul-Soo.
Soluble Fas ligand inhibits angiogenesis in rheumatoid arthritis ,
Arthritis Res Ther
 9 , R42 , 2007
Cells
Synoviocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human, 26-68 year
Marsano A, Millward-Sadler SJ, Salter DM, Adesida A, Hardingham T, Tognana E, Kon E, Chiari-Grisar C, Nehrer S, Jakob M, Martin I.
Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes ,
Osteoarthritis Cartilage
 15 , 48-58 , 2007
Cells
Meniscus and cartilage
Enzymes
Medium
DMEM
Cartilage
Human
Human, 64-83 yr
Tallheden T, Bengtsson C, Brantsing C, Sjogren-Jansson E, Carlsson L, Peterson L, Brittberg M. and Lindahl A.
Proliferation and Differentiation Potential of Chondrocytes from Osteoarthritic Patients ,
Arthritis Res Ther
 7(3) , R560 , 2005
Cells
Chondrocytes
Enzymes
Medium
DMEM/F12
Cartilage
Human
Human, adult
Liagre B, Vergne-Salle P, Corbiere C, Charissoux JL. and Beneytout JL.
Diosgenin, a Plant Steroid, Induces Apoptosis in Human Rheumatoid Arthritis Synoviocytes with Cyclooxygenase-2 Overexpression ,
Arthritis Res Ther
 6(4) , R373 , 2004
Cells
Synovial
Enzymes
Medium
DMEM
Cartilage
Human
Human, adult
Jakob M, Demarteau O, Schafer D, Stumm M, Heberer M. and Martin I.
Enzymatic Digestion of Adult Human Articular Cartilage Yields a Small Fraction of the Total Available Cells ,
Conn Tissue Res
 44 , 173 , 2003
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human, adults
McEvoy A. , Murphy E. , Ponnio T. , Conneely O. , Bresnihan B. , FitzGerald O. and Murphy E.
Activation of Nuclear Orphan Receptor NURR1 Transcription by NF-kappa B and Cyclic Adenosine 5'-Monophosphate Response Element-binding Protein in Rheumatoid Arthritis Synovial Tissue ,
J Immunol
 168(6) , 2979 , 2002
Cells
Synoviocytes
Enzymes
Medium
RPMI
Cartilage
Human
Human, 15-60 years
Rotter N, Bonassar LJ, Tobias G, Lebl M, Roy AK, Vacanti CA.
Age dependence of cellular properties of human septal cartilage: implications for tissue engineering ,
Arch Otolaryngol Head Neck Surg
 127 , 1248-52 , 2001
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human
Sarkissian M, Lafyatis R.
Integrin engagement regulates proliferation and collagenase expression of rheumatoid synovial fibroblasts ,
J Immunol
 162 , 1772-9 , 1999
Cells
Synovial fibroblasts
Enzymes
Collagenase : 0.4%
Medium
DMEM
Cartilage
Human
Human
Dunham B P, Koch R J.
Basic fibroblast growth factor and insulinlike growth factor I support the growth of human septal chondrocytes in a serum-free environment ,
Arch Otolaryngol Head Neck Surg
 124 , 1325-30 , 1998
Cells
Septal chondrocytes
Enzymes
Medium
DMEM/F12
Cartilage
Human
Human w/ rheumatoid arthritis
Dayer, J. , Krane, S. , Russell, R. and Robinson, D.
Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells ,
Proc Natl Acad Sci U S A
 73 (3) , 945 , 1976
Cells
Synovial tissue
Enzymes
Trypsin : 0.05%
Medium
DMEM
(see reference)
Cartilage
Human
Human, 13-62 years
Srivastava, V.M.L. , Malemud, C.J. , Hough, A.J. , Bland, J.H. and Sokoloff, L.
Preliminary Experience with Cell Culture of Human Articular Chondrocytes ,
Arthritis Rheum
 17 , 165 , 1974
Cells
Articular chondrocytes
Enzymes
Trypsin : 0.2%
Medium
BSS
Cartilage
Human
Human, 26-84 years
Manning, W.K. and Bonner, W.M.
Isolation and Culture of Chondrocytes From Human Adult Articular Cartilage ,
Arthritis Rheum
 10 , 235 , 1967
Cells
Chondrocytes
Medium
GBSS
Pancreas
Human
Human
Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L.
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. ,
Cell Rep
 22 , 2667-2676 , 2018
Cells
Islets
Enzymes
Collagenase Type 1 : 1,600u/isolation
Neutral Protease : 200 u/isolation
Deoxyribonuclease I : 12,000 u/isolation
Medium
RPMI
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Human
Human
Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D.
Identification of Pancreatic Cancer Stem Cells. ,
Cancer Res
 67 , 1030 , 2007
Cells
Pancreatic cancer stem cells
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
medium 199
Pancreas
Human
Human, 25-55 yrs old
(also porcine)
Contractor, H. , Johnson, P. , Chadwick, D. , Robertson, G. and London, N.
The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas ,
Cell Transplant
 4 , 615 , 1995
Cells
Islets
Enzymes
Collagenase : 0.4%
Medium
HBSS
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Pancreas
Human
Human, adult organ donors
Warnock, G. , Rajotte, R. , Evans, M. , Ellis, D. , DeGroot, T. and Dawidson, I.
Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas ,
Transplant Proc
 XIX (4) , 3466 , 1987
Cells
Islets
Enzymes
Collagenase : 0.6%
Medium
Eurocollins solution
Pancreas
Human
Human
Izumi, R. , Konishi, K. , Ueno, K. , Shimizu, K. , Hirosawa, H. , Takahashi, N. and Miyazaki, I.
Isolation of Human Pancreatic Islets from Cryopreserved Pancreas ,
Transplant Proc
 XVII , 383 , 1985
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human
Gray, D. , McShane, P. , Grant, A. and Morris, P.
A Method for Isolation of Islets of Langerhans from the Human Pancreas ,
Diabetes
 33 , 1055 , 1984
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human, infant, age 1 day-1 year
Sutherland, D. , Matas, A. , Steffes, M. and Najarian, J.
Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation ,
Diabetes
 25 (12) , 1123 , 1976
Cells
Islets
Enzymes
Collagenase : 170-210 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.