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Tissue References Search Tool

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Epithelial
Human
Human
Gottipamula, S. , Saraswat, S. and Sridhar, K.
Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells. ,
Cytotherapy
 19 , 263-271 , 2017
Cells
Epithelial
Enzymes
Medium
PBS
Epithelial
Human
Human
Hsueh, Y. , Huang, S. , Lai, J. , Ma, S. , Chen, H. , Wu, S. , Wang, T. , Sun, C. , Ma, K. , Chen, J. , Lai, C. and Ma, D.
Preservation of Epithelial Progenitor Cells from Collagenase-Digested Oral Mucosa During Ex Vivo Cultivation. ,
Sci Rep
 6 , 36266 , 2016
Cells
Oral mucosal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Human
Human
VanDussen, K. , Marinshaw, J. , Shaikh, N. , Miyoshi, H. , Moon, C. , Tarr, P. , Ciorba, M. and Stappenbeck, T.
Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. ,
Gut
 64 , 911-20 , 2015
Cells
Gastrointestinal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Human
Human
Widdicombe JH, Sachs LA, Morrow JL. and Finkbeiner WE.
Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function ,
Biotechniques
 39(2) , 249 , 2005
Cells
Human tracheal epithelium
Enzymes
Protease Type XIV : 0.04%
Medium
DMEM/F12
Epithelial
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Epithelial
Human
Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC
Novel enzymatic isolation of an entire viable human limbal epithelial sheet ,
Inv Ophthal Visual Sci
 44(10) , 4275 , 2003
Cells
Corneal limbal epithelial sheet
Enzymes
Medium
see reference
Epithelial
Human
Human
Fahlgren, A. , Hammarstrom, S. , Danielsson, A. and Hammarstrom, M.
Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. ,
Clin Exp Immunol
 131 , 90 , 2003
Cells
Intestinal epithelial
Enzymes
Collagenase Type 4 : 72.5 u/ml
Medium
HBSS
Epithelial
Human
Human
Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T.
A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells ,
Meth Cell Sci
 22 , 133 , 2000
Cells
Gastric epithelial cells
Enzymes
Medium
L-15
Epithelial
Human
Human
Halbert CL, Alexander IE, Wolgamot GM, Miller AD.
Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells ,
J Virol
 69 , 1473-9 , 1995
Cells
Nasal polyp epithelial
Enzymes
Trypsin : 0.1%
Medium
see reference
Epithelial
Human
Human
Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R. and Little, C
The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:10.1 , 1995
Cells
Gastric
Enzymes
Medium
F-12 medium
Epithelial
Human
Human
Sabatini, L. , Allen-Hoffmann, B, Warner, T. and Azen, E.
Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands ,
In Vitro Cell Dev Biol
 27A , 939 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Epithelial
Human
Human
Robinson, C. and Wu, R.
Culture of Conducting Airway Epithelial Cells in Serum-Free Medium ,
J Tiss Cul Meth
 13 , 95 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.2%
Medium
MEM, PBS
Epithelial
Human
Human
Emerman, J. and Wilkinson, D.
Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
 26 , 1186 , 1990
Cells
Epithelial
Enzymes
Collagenase : 2.0%
Medium
DMEM/Ham's F-12
Epithelial
Human
Human
Gruenert, D.C. , Basbaum, C.B. and Widdicombe, J.H.
Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions ,
In Vitro Cell Dev Biol
 26 , 411 , 1990
Cells
Epithelial
Enzymes
Pronase : 0.1%
Medium
PBS
Epithelial
Human
Human
Wood, L. and Neufeld, E.
A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux ,
J Biol Chem
 265 , 12796 , 1990
Cells
Epithelial
Sweat gland
Enzymes
Medium
(see reference)
Epithelial
Human
Human
(also bovine)
Munson, L. , Chandler, S. and Schlafer, D.
Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells ,
J Tiss Cul Meth
 11 (3) , 129 , 1988
Cells
Endometrial epithelial
Medium
DMEM/Ham's F-12
Epithelial
Human
Human
Widdicombe, J.H. , Coleman, D.L. , Finkbeiner, W.E. and Tuet, I.K.
Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa ,
J Appl Physiol
 58 , 1729 , 1985
Cells
Epithelial
Enzymes
Medium
HEPES with 5.9mM Glucose,5mM DTT
Epithelial
Human
Human
Yankaskas, J. , Cotton, C. , Knowles, M. , Gatzy, J. and Boucher, R.
Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports ,
Am Rev Respir Dis
 132 , 1281 , 1985
Cells
Epithelial
Enzymes
Protease Type XIV : 0.1%
Medium
Eagle's MEM
Epithelial
Human
Human, women, 27-49 years
Auersperg, N. , Siemens, C.H. and Myrdal, S.E.
Human Ovarian Surface Epithelium In Primary Culture ,
In Vitro
 20 , 743 , 1984
Cells
Epithelial
Ovary
Enzymes
Trypsin : 0.125%
Medium
HBSS, CMF
Epithelial
Human
Human, infant and neonate
Lechner, J. , Babcock, M. , Marnell, M. , Narayan, K. and Kaighn, M.
Normal Human Prostate Epithelial Cell Cultures ,
Methods Cell Biol
 21 , 195 , 1980
Cells
Epithelial
Prostate
Enzymes
Trypsin : 0.1%
Medium
HBSS
Reproductive
Human
Human
Chen, J. and Roan, N.
Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts. ,
Bio Protoc
 5 , 2015
Cells
Endometrial epithelial and stromal fibroblasts
Enzymes
Hyaluronidase : 62 u/ml
Medium
DMEM
Reproductive
Human
Human
Evans, J. and Salamonsen, L.
Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade. ,
Biol Reprod
 90 , 14 , 2014
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Reproductive
Human
Human
Patel, M. , Ghosh, M. , Fahey, J. and Wira, C.
Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol. ,
PLoS ONE
 7 , e35654 , 2012
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
Reproductive
Human
Human
Steigman, S. and Fauza, D.
Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1E.2 , 2007
Cells
Mesenchymal stem
Enzymes
Neutral Protease : see reference
Medium
CMF-DPBS
Reproductive
Human
Human, female
Lockwood, C. , Arcuri, F. , Toti, P. , Felice, C. , Krikun, G. , Guller, S. , Buchwalder, L. and Schatz, F.
Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis. ,
Am J Pathol
 169 , 1294-302 , 2006
Cells
Decidual
Enzymes
Collagenase : 0.25%
Deoxyribonuclease I : 6.25 u/ml
Medium
DMEM/F12
Reproductive
Human
Human
Spessotto, P. , Bulla, R. , Danussi, C. , Radillo, O. , Cervi, M. , Monami, G. , Bossi, F. , Tedesco, F. , Doliana, R. and Colombatti, A.
EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall. ,
J Cell Sci
 119 , 4574 , 2006
Cells
Extravillanous trophoblasts and decidual stromal
Enzymes
Trypsin : 0.1-0.25%
Deoxyribonuclease I : 0.02-0.05%
Medium
HBSS RPMI
Reproductive
Human
Human, female
Meter, R. , Wira, C. and Fahey, J.
Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture. ,
Fertil Steril
 84 , 191 , 2005
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun.
Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro ,
Chin Med J (Engl)
 118 , 1857-62 , 2005
Cells
Sertoli cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Wang Xin, Athayde Neil, Trudinger Brian.
Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease ,
Am J Obstet Gynecol
 190 , 596-601 , 2004
Cells
Endothelial placental
Enzymes
Medium
DMEM
Reproductive
Human
Human, female, 34-51 yr
Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E.
Clonogenicity of human endometrial epithelial and stromal cells ,
Biol Reprod
 70 , 1738-50 , 2004
Cells
Endometrial epithelial and stromal cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Sun Kang, Myatt Leslie.
Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts ,
Endocrinology
 144 , 5568-77 , 2003
Cells
Amnion epithelial and fibroblast
Enzymes
Trypsin : 0.125%
Collagenase : 0.1%
Medium
PBS
Reproductive
Human
Human
Fahey John V, Wira Charles R.
Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture ,
J Infect Dis
 185 , 1606-13 , 2002
Cells
Uterine epithelial cells
Enzymes
Pancreatin : 0.34%
Collagenase : 0.16%
Hyaluronidase : 0.16%
Medium
HBSS
Reproductive
Human
Human, embryo
Oberlin, E. , Tavian, M. , Blazsek, I. and Peault, B.
Blood-forming potential of vascular endothelium in the human embryo ,
Development
 129 , 4147 , 2002
Cells
Endothelial
Hematopoietic
Stromal
Enzymes
Medium
DMEM
Reproductive
Human
Human, female
Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S.
Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells ,
Hum Reprod
 17 , 2832-8 , 2002
Cells
Mesothelial
Enzymes
Medium
Eagle's MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.