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Tissue References Search Tool

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Mammary
Human
Human
Labarge, M. , Garbe, J. and Stampfer, M.
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture. ,
J Vis Exp
 71 , 2013
Cells
Mammary epithelial
Enzymes
Collagenase : 200 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F-12
Mammary
Human
Human, normal biopsy
Ronnov-Jessen L. , Villadsen R. , Edwards J. and Petersen O.
Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts ,
Am J Pathol
 161 , 471 , 2002
Cells
Fibroblasts
Enzymes
Collagenase Type 3 : 900 u/ml
Medium
DMEM/F-12
Mammary
Human
Human
Huss, F. and Kratz, G.
Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue. ,
Cells Tissues Organs
 169 , 361-7 , 2001
Cells
Mammary epithelial cells
Enzymes
Medium
DMEM
Mammary
Human
Human
Ogmundsdottir, H. , Petursdottir, I. , Gudmundsdottir, I. , Amundadottir, L. , Ronnov-Jessen, L. and Petersen, O.
Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures ,
In Vitro Cell Dev Biol
 29A , 936 , 1993
Cells
Epithelial
Fibroblasts
Enzymes
Collagenase Type 1 : 450 IU/ml
Medium
DMEM/Ham's F-12
Mammary
Human
Human
Ronnov-Jessen, L. , VanDeurs, B. , Nielsen, M. and Petersen, O.W.
Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture ,
In Vitro Cell Dev Biol
 28 , 273 , 1992
Cells
Myofibroblasts
Enzymes
Collagenase : 900 IU/ml
Medium
DME - F12
Mammary
Human
Human, female, 15-61 yrs old
Berthon, P. , Pancino, G. , Cremoux, P. , Roseto, A. , Gespach, C. and Calvo, F.
Characterization of Normal Breast Epithelial Cells in Primary Cultures: Differentiation and Growth Factor Receptors Studies ,
J Tiss Cul Meth
 28A , 716 , 1992
Cells
Epithelial
Enzymes
Hyaluronidase : 150 IU/ml
Medium
DMEM
Mammary
Human
Human
Emerman, J. and Wilkinson, D.
Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
 26 , 1186 , 1990
Cells
Epithelial
Enzymes
Collagenase : 2.0%
Medium
DMEM/Ham's F-12
Mammary
Human
Human
Leung, C. and Shiu, R.
Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix ,
In Vitro
 18 , 476 , 1981
Cells
Tumor, breast
Enzymes
Neuraminidase : 0.8 u/ml
Medium
HBSS
Mammary
Human
Human
Stampfer, M. , Hallowes, R. and Hackett, A.
Growth of Normal Human Mammary Cells in Culture ,
In Vitro
 16 (5) , 415 , 1980
Cells
Epithelial
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM/Ham's F-12
Pancreas
Human
Human
Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L.
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. ,
Cell Rep
 22 , 2667-2676 , 2018
Cells
Islets
Enzymes
Collagenase Type 1 : 1,600u/isolation
Neutral Protease : 200 u/isolation
Deoxyribonuclease I : 12,000 u/isolation
Medium
RPMI
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Human
Human
Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D.
Identification of Pancreatic Cancer Stem Cells. ,
Cancer Res
 67 , 1030 , 2007
Cells
Pancreatic cancer stem cells
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
medium 199
Pancreas
Human
Human, 25-55 yrs old
(also porcine)
Contractor, H. , Johnson, P. , Chadwick, D. , Robertson, G. and London, N.
The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas ,
Cell Transplant
 4 , 615 , 1995
Cells
Islets
Enzymes
Collagenase : 0.4%
Medium
HBSS
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Pancreas
Human
Human, adult organ donors
Warnock, G. , Rajotte, R. , Evans, M. , Ellis, D. , DeGroot, T. and Dawidson, I.
Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas ,
Transplant Proc
 XIX (4) , 3466 , 1987
Cells
Islets
Enzymes
Collagenase : 0.6%
Medium
Eurocollins solution
Pancreas
Human
Human
Izumi, R. , Konishi, K. , Ueno, K. , Shimizu, K. , Hirosawa, H. , Takahashi, N. and Miyazaki, I.
Isolation of Human Pancreatic Islets from Cryopreserved Pancreas ,
Transplant Proc
 XVII , 383 , 1985
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human
Gray, D. , McShane, P. , Grant, A. and Morris, P.
A Method for Isolation of Islets of Langerhans from the Human Pancreas ,
Diabetes
 33 , 1055 , 1984
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human, infant, age 1 day-1 year
Sutherland, D. , Matas, A. , Steffes, M. and Najarian, J.
Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation ,
Diabetes
 25 (12) , 1123 , 1976
Cells
Islets
Enzymes
Collagenase : 170-210 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.