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Tissue References Search Tool

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Colon
Human
Human
Moser, A. , Spindelboeck, W. , Strohmaier, H. , Enzinger, C. , Gattringer, T. , Fuchs, S. , Fazekas, F. , Gorkiewicz, G. , Wurm, P. , Hogenauer, C. and Khalil, M.
Mucosal Biopsy Shows Immunologic Changes of the Colon in Patients with Early MS ,
Neurol Neuroimmunol Neuroinflamm
 4 , e362 , 2017
Cells
Lamina propria mononuclear
Enzymes
Medium
HBSS
Colon
Human
Human
Rollo, B. , Zhang, D. , Stamp, L. , Menheniott, T. , Stathopoulos, L. , Denham, M. , Dottori, M. , King, S. , Hutson, J. and Newgreen, D.
Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon ,
Cell Mol Gastroenterol Hepatol
 2 , 92 , 2016
Cells
Colon enteric neural
Enzymes
Medium
Ham's F-12
Colon
Human
Human
Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
 , e52532 , 2015
Cells
Colon
Enzymes
Trypsin : 0.1%
Collagenase : 0.1%
Medium
HBSS
Colon
Human
Human
VanDussen, K. , Marinshaw, J. , Shaikh, N. , Miyoshi, H. , Moon, C. , Tarr, P. , Ciorba, M. and Stappenbeck, T.
Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. ,
Gut
 64 , 911-20 , 2015
Cells
Gastrointestinal epithelial
Enzymes
Medium
DMEM/F12
Colon
Human
Human
Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D.
Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. ,
J Physiol
 591 , 5959-75 , 2013
Cells
Enterochromaffin
Enzymes
Trypsin : 0.05%
Medium
DMEM
Colon
Human
Human
Roig, A. , Eskiocak, U. , Hight, S. , Kim, S. , Delgado, O. , Souza, R. , Spechler, S. , Wright, W. and Shay, J.
Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. ,
Gastroenterol
 138 , 1012 , 2010
Cells
Colonic epithelial
Enzymes
Collagenase : 150 u/ml
Neutral Protease : 0.04 mg/ml
Medium
Basal X media
Colon
Human
Human
Zhou, J. , Belov, L. , Huang, P. , Shin, J. , Solomon, M. , Chapuis, P. , Bokey, L. , Chan, C. , Clarke, C. , Clarke, S. and Christopherson, R.
Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing. ,
J Immunol Methods
 355 , 40 , 2010
Cells
Colorectal cancer
Enzymes
Medium
HBSS
Colon
Human
Human
Huang, E. , Hynes, M. , Zhang, T. , Ginestier, C. , Dontu, G. , Appelman, H. , Fields, J. , Wicha, M. and Boman, B.
Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis. ,
Cancer Res
 69 , 3382-9 , 2009
Cells
Colonic epithelial
Enzymes
Medium
not listed
Colon
Human
Human
Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A.
Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. ,
EMBO Mol Med
 1 , 338-51 , 2009
Cells
Colon cancer
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F12
Colon
Human
Human
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.
Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
 104 , 10158-63 , 2007
Cells
Cancer stem cell
Enzymes
Collagenase Type 3 : 200 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
RPMI-1640
Colon
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Colon
Human
Human
Wang D. , Lehman R. , Donner D. , Matli M. , Warren R. and Welton M.
Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells ,
Am J Physiol/Gastro
 282 , G1088 , 2002
Cells
Colonic endothelial cells
Enzymes
Medium
HBSS/5%FBS
Colon
Human
Human
Emenaker N, Calaf G, Cox D, Basson M. and Qureshi N
Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes ,
J Nutr
 46 , 96-105 , 2001
Cells
Colonocytes
Enzymes
Collagenase : 0.15%
Medium
DMEM/F12
Colon
Human
Human
Hisamatsu, T. , Watanabe, M. , Ogata, H. , Ezaki, T. , Hozawa, S. , Ishii, H. , Kanai, T. and Hibi, T.
Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. ,
Cancer Res
 59 , 5927-31 , 1999
Cells
Epithelial and mucosal lymphocytes
Enzymes
CLSPA : 0.02%
Medium
RPMI 1640
Colon
Human
Human
Gibson, P. , Rosella, O. , Wilson, A. , Mariadason, J. , Rickard, K. , Byron, K. and Barkla, D.
Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate. ,
Carcinogenesis
 20 , 539 , 1999
Cells
Colonic epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase Type 4 : 50 u/ml
Medium
HBSS
Colon
Human
Human
Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y.
High Fas ligand expression on lymphocytes in lesions of ulcerative colitis ,
Gut
 43 , 48-55 , 1998
Cells
Lamina propria lymphocytes
Enzymes
Collagenase : 25 u/ml
Medium
HBSS
Reproductive
Human
Human
Chen, J. and Roan, N.
Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts. ,
Bio Protoc
 5 , 2015
Cells
Endometrial epithelial and stromal fibroblasts
Enzymes
Hyaluronidase : 62 u/ml
Medium
DMEM
Reproductive
Human
Human
Evans, J. and Salamonsen, L.
Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade. ,
Biol Reprod
 90 , 14 , 2014
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Reproductive
Human
Human
Patel, M. , Ghosh, M. , Fahey, J. and Wira, C.
Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol. ,
PLoS ONE
 7 , e35654 , 2012
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
Reproductive
Human
Human
Steigman, S. and Fauza, D.
Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1E.2 , 2007
Cells
Mesenchymal stem
Enzymes
Neutral Protease : see reference
Medium
CMF-DPBS
Reproductive
Human
Human, female
Lockwood, C. , Arcuri, F. , Toti, P. , Felice, C. , Krikun, G. , Guller, S. , Buchwalder, L. and Schatz, F.
Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis. ,
Am J Pathol
 169 , 1294-302 , 2006
Cells
Decidual
Enzymes
Collagenase : 0.25%
Deoxyribonuclease I : 6.25 u/ml
Medium
DMEM/F12
Reproductive
Human
Human
Spessotto, P. , Bulla, R. , Danussi, C. , Radillo, O. , Cervi, M. , Monami, G. , Bossi, F. , Tedesco, F. , Doliana, R. and Colombatti, A.
EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall. ,
J Cell Sci
 119 , 4574 , 2006
Cells
Extravillanous trophoblasts and decidual stromal
Enzymes
Trypsin : 0.1-0.25%
Deoxyribonuclease I : 0.02-0.05%
Medium
HBSS RPMI
Reproductive
Human
Human, female
Meter, R. , Wira, C. and Fahey, J.
Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture. ,
Fertil Steril
 84 , 191 , 2005
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun.
Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro ,
Chin Med J (Engl)
 118 , 1857-62 , 2005
Cells
Sertoli cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Wang Xin, Athayde Neil, Trudinger Brian.
Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease ,
Am J Obstet Gynecol
 190 , 596-601 , 2004
Cells
Endothelial placental
Enzymes
Medium
DMEM
Reproductive
Human
Human, female, 34-51 yr
Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E.
Clonogenicity of human endometrial epithelial and stromal cells ,
Biol Reprod
 70 , 1738-50 , 2004
Cells
Endometrial epithelial and stromal cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Sun Kang, Myatt Leslie.
Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts ,
Endocrinology
 144 , 5568-77 , 2003
Cells
Amnion epithelial and fibroblast
Enzymes
Trypsin : 0.125%
Collagenase : 0.1%
Medium
PBS
Reproductive
Human
Human
Fahey John V, Wira Charles R.
Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture ,
J Infect Dis
 185 , 1606-13 , 2002
Cells
Uterine epithelial cells
Enzymes
Pancreatin : 0.34%
Collagenase : 0.16%
Hyaluronidase : 0.16%
Medium
HBSS
Reproductive
Human
Human, embryo
Oberlin, E. , Tavian, M. , Blazsek, I. and Peault, B.
Blood-forming potential of vascular endothelium in the human embryo ,
Development
 129 , 4147 , 2002
Cells
Endothelial
Hematopoietic
Stromal
Enzymes
Medium
DMEM
Reproductive
Human
Human, female
Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S.
Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells ,
Hum Reprod
 17 , 2832-8 , 2002
Cells
Mesothelial
Enzymes
Medium
Eagle's MEM
Reproductive
Human
Human
Zhang, S. , Wernig, M. , Duncan, I. , Brustle, O. and Thomson, J.
In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells ,
Nat Biotechnol
 19 , 1129 , 2001
Cells
Stem, embryonic
Enzymes
Neutral Protease : 0.01% - 0.02%
Medium
DMEM
Reproductive
Human
Human, 20-40 yr
Arnold, J. , Kaufman, D. , Seppala, M. and Lessey, B.
Endometrial stromal cells regulate epithelial cell grownth in vitro: a new co-culture model ,
Hum Reprod
 16 , 836 , 2001
Cells
Endometrium epithelial and stromal cells
Enzymes
Medium
HBSS
Reproductive
Human
Human
Yusuf RZ, Naeem R.
Cytogenetic studies of spontaneous miscarriages: a seven year study to compare significance of primary vs. secondary culture methods for assessment of fetal karyotype yield and maternal cell contamination ,
Early Pregnancy
 5 , 121-31 , 2001
Cells
Chorionic villi
Enzymes
Trypsin : see reference
Collagenase Type 3 : 100 u/ml
Medium
HBSS
Reproductive
Human
Human, female, 20-40 year
Gargett CE, Bucak K, Rogers PA.
Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells ,
Hum Reprod
 15 , 293-301 , 2000
Cells
Microvascular endothelial cells
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.