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Tissue References Search Tool

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Reproductive
Mouse
Mouse, adult, neonatal and fetal
O'Shaughnessy PJ, Fleming LM, Jackson G, Hochgeschwender U, Reed P, Baker PJ.
Adrenocorticotropic hormone directly stimulates testosterone production by the fetal and neonatal mouse testis ,
Endocrinology
 144 , 3279-84 , 2003
Cells
Testicular cells
Enzymes
Medium
DMEM/F-12
Reproductive
Mouse
Mouse, 6 day
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta.
Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse ,
Biol Reprod
 69 , 985-94 , 2003
Cells
Sertoli
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, male, 3.5 months old
Faldikova, L. , Diblikova, I. , Canderle, J. , Zraly, Z. , Veznik, Z. and Sulcova, A.
Effects of Nutrition, Social Factors and Chronic Stress on the Mouse Leydig Cell Testosterone Production ,
Vet Med
 46 (6) , 160 , 2001
Cells
Leydig
Enzymes
Collagenase : 0.06%
Medium
Medium E 199
Reproductive
Mouse
Mouse, male, 12-14-week-old
Lin, Q. , Sirotkin, A. and Skoultchi, A.
Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t ,
Mol Cell Biol
 20 (6) , 2122 , 2000
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, 6-12 day
Eppig JJ, O'Brien MJ.
Development in vitro of mouse oocytes from primordial follicles ,
Biol Reprod
 54 , 197-207 , 1996
Cells
Oocyte-granulosa
Enzymes
Medium
Waymouth
Reproductive
Mouse
Mouse, female,6-8 weeks
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
 27A , 713 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
HBSS
Reproductive
Mouse
Mouse, female, 21 day, 10-12 g
Tsai, P. , Uchima, F. , Hamamoto, S. and Bern, H.
Proliferation and Differentiation of Prepubertal Mouse Vaginal Epithelial Cells In Vitro and the Specificity of Estrogen-Induced Growth Retardation ,
In Vitro Cell Dev Biol
 27A , 461 , 1991
Cells
vaginal epithelial
Enzymes
Collagenase Type 3 : 38 u/ml
Medium
DMEM
Reproductive
Mouse
Mouse, CF1, female
Spindle, A.
In vitro Development of One-Cell Embryos from Outbred Mice: Influence of Culture Medium Composition ,
In Vitro Cell Dev Biol
 26 , 151 , 1990
Cells
Cumulus, one-cell embryos
Enzymes
Hyaluronidase : 0.1%
Medium
PBS, CMF
Reproductive
Mouse
Mouse (BALB/cCRGL), male, 2-3 months
Turner, T. , Bern, H. , Young,P. and Cunha, G.
Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel ,
In Vitro Cell Dev Biol
 26 , 722 , 1990
Cells
Epithelial, prostate gland
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Reproductive
Mouse
Mouse (C57B/T), fetus, 16-17 days old
Thompson, T.C. , Southgate, J. , Kitchener, G. and Land, H.
Multistage Carcinogenesis Induced by ras and myc Oncogenes in a Reconstituted Organ ,
Cell
 56 , 917 , 1989
Cells
Prostate
Enzymes
Trypsin : 1.0%
Medium
HBSS/ DMEM
Reproductive
Mouse
Mouse, outbred, CD-1, 21-23 days old
Tomooka, Y. , DiAugustine, R. and McLachlan, J.
Proliferation of Mouse Uterine Epithelial Cells in Vitro ,
Endocrinology
 118 (3) , 1011 , 1986
Cells
Epithelial
Enzymes
Trypsin : 0.5%
Medium
Medium 199
Reproductive
Mouse
Mouse, BALB/c
Bigsby, R. , Cooke, P. and Cunha, G.
A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells ,
Am J Physiol
 251 , E630 , 1986
Cells
Epithelial
Mesencymal
Enzymes
Trypsin : 1%
Medium
DMEM
Reproductive
Mouse
Mouse, BALB/cCrg, 40 days old
Cooke, P. , Uchima, F. , Fujii, D. , Bern, H. and Cunha, G.
Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma ,
Proc Natl Acad Sci U S A
 83 , 2109 , 1986
Cells
Epithelial
Enzymes
Trypsin : 1%
Medium
Medium 199
Reproductive
Mouse
Mouse, male, 10-13 wk
Stalvcey, J. and Payne, A.
Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains ,
Endocrinology
 112 , 1696 , 1983
Cells
Leydig
Testis
Enzymes
Medium
Medium 199 w/ BSA
Reproductive
Mouse
Mouse (BALB/cCrgl), female, 50 - 60 days
Iguchi, T. , Uchima, F.A. , Ostrander, P. and Bern, H.
Growth of Normal Mouse Vaginal Epithelial Cells in and on Collagen Gels ,
Proc Natl Acad Sci U S A
 80 , 3743 , 1983
Cells
Epithelial, vagina
Enzymes
Medium
HBSS
Reproductive
Ovine
Sheep
Salamonsen, L. , Sum O, W. , Doughton, B. and Findlay, J.
The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium ,
Endocrinology
 117 (5) , 2148 , 1985
Cells
Epithelial
Enzymes
Collagenase : 125 - 190 u/ml
Medium
DMEM
Skin
Mouse
Mouse
Spidale, N. , Malhotra, N. , Frascoli, M. , Sylvia, K. , Miu, B. , Freeman, C. , Stadinski, B. , Huseby, E. and Kang, J.
Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent Spontaneous Atopic Dermatitis ,
Elife
 9 , 5118 , 2020
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
Skin
Mouse
Mouse
Rinkevich, Y. , Walmsley, G. , Hu, M. , Maan, Z. , Newman, A. , Drukker, M. , Januszyk, M. , Krampitz, G. , Gurtner, G. , Lorenz, H. , Weissman, I. and Longaker, M.
Identification and Isolation of a Dermal Lineage with Intrinsic Fibrogenic Potential. ,
Science
 348 , aaa2151 , 2015
Cells
Dermal fibroblasts
Enzymes
Elastase : 0.012%
Medium
DMEM
Skin
Mouse
Mouse
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Mouse
Mouse
Jensen, U. , Ghazizadeh, S. and Owens, D.
Isolation and Characterization of Cutaneous Epithelial Stem Cells. ,
Methods Mol Biol
 989 , 61-9 , 2013
Cells
Epithelial stem
Enzymes
Trypsin : 0.25%
Medium
HBSS
Skin
Mouse
Mouse
Gu, D. , Fan, Q. and Xie, J.
Cell Population Analyses During Skin Carcinogenesis. ,
J Vis Exp
 , e50311 , 2013
Cells
Epidermal
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Antsiferova, M. , Martin, C. , Huber, M. , Feyerabend, T. , Forster, A. , Hartmann, K. , Rodewald, H. , Hohl, D. and Werner, S.
Mast Cells are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis. ,
J Immunol
 191 , 6147-55 , 2013
Cells
Mast
Enzymes
Medium
IMDM
Skin
Mouse
Mouse
Scheibye-Knudsen, M. , Ramamoorthy, M. , Sykora, P. , Maynard, S. , Lin, P. , Minor, R. , Wilson, D. , Cooper, M. , Spencer, R. , de Cabo, R. , Croteau, D. and Bohr, V.
Cockayne Syndrome Group B Protein Prevents the Accumulation of Damaged Mitochondria by Promoting Mitochondrial Autophagy. ,
J Exp Med
 209 , 855 , 2012
Cells
Dermal fibroblasts
Enzymes
Collagenase : 400 u/ml
Medium
AminoMAX II
Skin
Mouse
Mouse, 2-3 day
Farina, G. , York, M. , Di Marzio, M. , Collins, C. , Meller, S. , Homey, B. , Rifkin, I. , Marshak-Rothstein, A. , Radstake, T. and Lafyatis, R.
Poly(I:C) Drives Type I IFN- and TGFβ-Mediated Inflammation and Dermal Fibrosis Simulating Altered Gene Expression in Systemic Sclerosis. ,
J Invest Dermatol
 130 , 2583-93 , 2010
Cells
Dermal fibroblasts
Enzymes
Trypsin : 0.025%
Medium
DME
Skin
Mouse
Mouse
King, I. , Kroenke, M. and Segal, B.
GM-CSF-Dependent, CD103+ Dermal Dendritic Cells Play a Critical Role in Th Effector Cell Differentiation After Subcutaneous Immunization. ,
J Exp Med
 207 , 953 , 2010
Cells
Epidermal and dermal
Enzymes
Medium
PBS
Skin
Mouse
Mouse
Eidsmo, L. , Allan, R. , Caminschi, I. , Van Rooijen, N. , Heath, W. and Carbone, F.
Differential Migration of Epidermal and Dermal Dendritic Cells During Skin Infection. ,
J Immunol
 182 , 3165 , 2009
Cells
Dermal
Enzymes
Medium
HBSS
Skin
Mouse
Mouse, neonatal
Crigler Lauren, Kazhanie Amita, Yoon Tae-Jin, Zakhari Julia, Anders Joanna, Taylor Barbara, Virador VictoriaM.
Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages ,
FASEB J
 21 , 2050-63 , 2007
Cells
Dermal
Enzymes
Collagenase : 0.35%
Deoxyribonuclease I : see reference
Medium
PBS
Skin
Mouse
mouse
Baxter Ruth M, Crowell Thomas P, McCrann Margaret E, Frew Erica M, Gardner Humphrey.
Analysis of the tight skin (Tsk1/+) mouse as a model for testing antifibrotic agents ,
Lab Invest
 85 , 1199-209 , 2005
Cells
Dermal fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Skin
Mouse
Mouse, male 6-24 week
Montanaro F, Liadaki K, Volinski J, Flint A. and Kunkel LM.
Skeletal muscle engraftment potential of adult mouse skin side population cells ,
Proc Natl Acad Sci U S A
 100 , 9336 , 2003
Cells
Skin side population
Enzymes
Medium
PBS
Skin
Mouse
Mouse
Takanami-Ohnishi Yoko, Amano Shinya, Kimura Sadao, Asada Sachie, Utani Atsushi, Maruyama Masumi, Osada Hiroyuki, Tsunoda Hajime, Irukayama-Tomobe Yoko, Goto Katsutoshi, Karin Michael, Sudo Tatsuhiko, Kasuya Yoshitoshi.
Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity ,
J Biol Chem
 277 , 37896-903 , 2002
Cells
Ear epidermal
Enzymes
Trypsin : 0.1%
Collagenase : 0.2%
Medium
PBS
Skin
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Villarreal, E. , Hewgley, W. , Lang, W. , Morton, C. , Mao, S. , Wu, J. and Sandoval, J.
In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation? ,
J Surg Res
 218 , 209-216 , 2017
Cells
Speen
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.