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Tissue References Search Tool

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Muscle
Mouse

Mouse

Ghaibour, K. , Rizk, J. , Ebel, C. , Ye, T. , Philipps, M. , Schreiber, V. , Metzger, D. and Duteil, D.
An Efficient Protocol for CUT&RUN Analysis of FACS-Isolated Mouse Satellite Cells. ,
J Vis Exp
 , 2023
Cells
Satellite cells
Enzymes
Medium
DMEM/F-12
Muscle
Mouse
Huttner,S. , Ahrens, H. , Schmidt, M. , Henze, H. , Jung, M. , Schuler. S. and von Maltzahn, J.
Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle. ,
Methods Mol Biol
 , 2019
Cells
Myofiber
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse
Eckhardt, J. , Bachmann, C. , Sekulic-Jablanovic, M. , Enzmann, V. , Park, K. , Ma, J. , Takeshima, H. , Zorzato, F. and Treves, S.
Extraocular Muscle Function is Impaired in ryr3−/− Mice ,
J Gen Physiol
 151 , 929-943 , 2019
Cells
Extraocular muscles
Enzymes
Elastase : 0.0125-0.1%
Collagenase Type 2 : 0.03-0.2%
Medium
Ringer
Muscle
Mouse

Mouse, adult

Altman, T. , Geller, D. , Kleeblatt, E. , Gradus-Perry, T. and Perlson, E.
An in vitro Compartmental System Underlines the Contribution of Mitochondrial Immobility to the ATP Supply in the NMJ. ,
J Cell Sci
 132 , 2019
Cells
Skeletal muscle
Enzymes
Medium
See reference
Muscle
Mouse
Mouse, 6-8 week
Sachdeva, J. , Mahajan, A. , Cheng, J. , Baeten, J. , Lilly, B. , Kuivaniemi, H. and Hans, C.
Smooth Muscle Cell-Specific Notch1 Haploinsufficiency Restricts the Progression of Abdominal Aortic Aneurysm by Modulating CTGF Expression. ,
PLoS ONE
 12 , e0178538 , 2017
Cells
vascular smooth muscle
Enzymes
Elastase : 0.75 u/ml
Medium
DMEM/F12
Muscle
Mouse
Mouse, GFP transgenic
Birbrair, A, Sattiraju, A, Zhu, D, Zulato, G, Batista, I, Nguyen, V, Messi M, Solingapuram Sai, K, Marini, F, Delbono, O. and Mintz, A.
Novel Peripherally Derived Neural-Like Stem Cells as Therapeutic Carriers for Treating Glioblastomas. ,
Stem Cells Transl Med
 , 2016
Cells
Muscle derived NLSC
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse, 2-4 month
Randolph, M. , Phillips, B. , Choo, H. , Vest, K. , Vera, Y. and Pavlath, G.
Pharyngeal Satellite Cells Undergo Myogenesis Under Basal Conditions and Are Required for Pharyngeal Muscle Maintenance. ,
Stem Cells
 33 , 3581-95 , 2015
Cells
Pharyngeal myofiber
Enzymes
Collagenase Type 1 : 400-800 u/ml
Medium
DMEM
Muscle
Mouse
Mouse, 2 month
Abou-Khalil, R. , Yang, F. , Lieu, S. , Julien, A. , Perry, J. , Pereira, C. , Relaix, F. , Miclau, T. , Marcucio, R. and Colnot, C.
Role of Muscle Stem Cells During Skeletal Regeneration. ,
Stem Cells
 33 , 1501-11 , 2015
Cells
Myoblasts
Enzymes
Collagenase Type 2 : 400 u/ml
Neutral Protease : 0.5 u/ml
Medium
Hams F-10
Muscle
Mouse
Mouse, 6-8 week
Pasut, A. , Jones, A. and Rudnicki, M.
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle. ,
J Vis Exp
 73 , e50074 , 2013
Cells
Myofibers
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse, 10 week
Rezk, B. , Yoshida, T. , Semprun-Prieto, L. , Higashi, Y. , Sukhanov, S. and Delafontaine, P.
Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy. ,
PLoS ONE
 7 , e30276 , 2012
Cells
Diaphragm cells
Enzymes
Collagenase Type 2 : 100 u/ml
Pronase : 0.125%
Medium
PBS
Muscle
Mouse
Mouse
Weisleder, N. , Zhou, J. and Ma, J.
Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers. ,
Methods Mol Biol
 798 , 395 , 2012
Cells
Skeletal muscle fiber
Enzymes
Medium
Tyrode
Muscle
Mouse
Mouse
Metz, R. , Patterson, J. and Wilson, E.
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization. ,
Methods Mol Biol
 843 , 169 , 2012
Cells
Vascular smooth muscle
Enzymes
Medium
Basal Culture
Muscle
Mouse
Mouse
Shi, H. , Boadu, E. , Mercan, F. , Le, A. , Roth Flach, R. , Zhang, L. , Tyner, K. , Olwin, B. and Bennett, A.
MAP Kinase Phosphatase-1 Deficiency Impairs Skeletal Muscle Regeneration and Exacerbates Muscular Dystrophy. ,
FASEB J
 24 , 2985 , 2010
Cells
Myoblast
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse, 1-25 month
Ieronimakis Nicholas, Balasundaram Gayathri, Reyes Morayma.
Direct isolation, culture and transplant of mouse skeletal muscle derived endothelial cells with angiogenic potential ,
PLoS ONE
 3 , e0001753 , 2008
Cells
Myocytes, endothelial
Enzymes
Medium
PBS
Muscle
Mouse
Mouse, neonatal
Johnson, B. , Scheuer, T. and Catterall, W.
Convergent Regulation of Skeletal Muscle Ca2+ Channels by Dystrophin, the Actin Cytoskeleton, and cAMP-Dependent Protein Kinase. ,
Proc Natl Acad Sci U S A
 102 , 4191 , 2005
Cells
Skeletal muscle myotubes
Enzymes
NCIS kit : per instructions
Medium
L-15
Muscle
Mouse
Mouse, male, 6-14 week
Winitsky, S. , Gopal, T. , Hassanzadeh, S. , Takahashi, H. , Gryder, D. , Rogawski, M. , Takeda, K. , Yu, Z. , Xu, Y. and Epstein, N.
Adult Murine Skeletal Muscle Contains Cells That Can Differentiate into Beating Cardiomyocytes In Vitro. ,
PLoS Biol
 3 , e87 , 2005
Cells
Precursor cells
Enzymes
Medium
DMEM/F12
Muscle
Mouse
Mouse, 15-20 day
Li CX, Liu BH, Tong WD, Zhang LY. and Jiang YP
Dissociation, culture and morphologic changes of interstitial cells of Cajal in vitro ,
World J Gastroenterol
 11 , 2838 , 2005
Cells
Intersitial cells of Cajal
Enzymes
Medium
M199
Muscle
Mouse
Mouse, 6-8 week
Majka S. , Jackson K. , Kienstra K. , Majesky M. , Goodell M. and Hirschi K.
Distinct Progenitor Populations in Skeletal Muscle Are Bone Marrow Derived and Exhibit Different Cell Fates During Vascular Regeneration ,
J Clin Invest
 111 , 71 , 2003
Cells
Skeletal muscle progenitor
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse, 6-8 week
McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.
Muscle-derived hematopoietic stem cells are hematopoietic in origin ,
Proc Natl Acad Sci U S A
 99 , 1341 , 2002
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
HBSS
Muscle
Mouse
Mouse, neonatal
Fukada S. , Miyagoe-Suzuki Y. , Tsukihara H. , Yuasa K. , Higuchi S. , Ono S. , Tsujikawa K. , Takeda S. and Yamamoto H.
Muscle Regeneration by Reconstitution with Bone Marrow or Fetal Liver Cells from Green Fluorescent Protein-gene Transgenic Mice ,
J Cell Sci
 115 , 1285 , 2002
Cells
Myocytes
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Spidale, N. , Malhotra, N. , Frascoli, M. , Sylvia, K. , Miu, B. , Freeman, C. , Stadinski, B. , Huseby, E. and Kang, J.
Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent Spontaneous Atopic Dermatitis ,
Elife
 9 , 5118 , 2020
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
Skin
Mouse
Mouse
Rinkevich, Y. , Walmsley, G. , Hu, M. , Maan, Z. , Newman, A. , Drukker, M. , Januszyk, M. , Krampitz, G. , Gurtner, G. , Lorenz, H. , Weissman, I. and Longaker, M.
Identification and Isolation of a Dermal Lineage with Intrinsic Fibrogenic Potential. ,
Science
 348 , aaa2151 , 2015
Cells
Dermal fibroblasts
Enzymes
Elastase : 0.012%
Medium
DMEM
Skin
Mouse
Mouse
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Mouse
Mouse
Jensen, U. , Ghazizadeh, S. and Owens, D.
Isolation and Characterization of Cutaneous Epithelial Stem Cells. ,
Methods Mol Biol
 989 , 61-9 , 2013
Cells
Epithelial stem
Enzymes
Trypsin : 0.25%
Medium
HBSS
Skin
Mouse
Mouse
Gu, D. , Fan, Q. and Xie, J.
Cell Population Analyses During Skin Carcinogenesis. ,
J Vis Exp
 , e50311 , 2013
Cells
Epidermal
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Antsiferova, M. , Martin, C. , Huber, M. , Feyerabend, T. , Forster, A. , Hartmann, K. , Rodewald, H. , Hohl, D. and Werner, S.
Mast Cells are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis. ,
J Immunol
 191 , 6147-55 , 2013
Cells
Mast
Enzymes
Medium
IMDM
Skin
Mouse
Mouse
Scheibye-Knudsen, M. , Ramamoorthy, M. , Sykora, P. , Maynard, S. , Lin, P. , Minor, R. , Wilson, D. , Cooper, M. , Spencer, R. , de Cabo, R. , Croteau, D. and Bohr, V.
Cockayne Syndrome Group B Protein Prevents the Accumulation of Damaged Mitochondria by Promoting Mitochondrial Autophagy. ,
J Exp Med
 209 , 855 , 2012
Cells
Dermal fibroblasts
Enzymes
Collagenase : 400 u/ml
Medium
AminoMAX II
Skin
Mouse
Mouse, 2-3 day
Farina, G. , York, M. , Di Marzio, M. , Collins, C. , Meller, S. , Homey, B. , Rifkin, I. , Marshak-Rothstein, A. , Radstake, T. and Lafyatis, R.
Poly(I:C) Drives Type I IFN- and TGFβ-Mediated Inflammation and Dermal Fibrosis Simulating Altered Gene Expression in Systemic Sclerosis. ,
J Invest Dermatol
 130 , 2583-93 , 2010
Cells
Dermal fibroblasts
Enzymes
Trypsin : 0.025%
Medium
DME
Skin
Mouse
Mouse
King, I. , Kroenke, M. and Segal, B.
GM-CSF-Dependent, CD103+ Dermal Dendritic Cells Play a Critical Role in Th Effector Cell Differentiation After Subcutaneous Immunization. ,
J Exp Med
 207 , 953 , 2010
Cells
Epidermal and dermal
Enzymes
Medium
PBS
Skin
Mouse
Mouse
Eidsmo, L. , Allan, R. , Caminschi, I. , Van Rooijen, N. , Heath, W. and Carbone, F.
Differential Migration of Epidermal and Dermal Dendritic Cells During Skin Infection. ,
J Immunol
 182 , 3165 , 2009
Cells
Dermal
Enzymes
Medium
HBSS
Skin
Mouse
Mouse, neonatal
Crigler Lauren, Kazhanie Amita, Yoon Tae-Jin, Zakhari Julia, Anders Joanna, Taylor Barbara, Virador VictoriaM.
Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages ,
FASEB J
 21 , 2050-63 , 2007
Cells
Dermal
Enzymes
Collagenase : 0.35%
Deoxyribonuclease I : see reference
Medium
PBS
Skin
Mouse
mouse
Baxter Ruth M, Crowell Thomas P, McCrann Margaret E, Frew Erica M, Gardner Humphrey.
Analysis of the tight skin (Tsk1/+) mouse as a model for testing antifibrotic agents ,
Lab Invest
 85 , 1199-209 , 2005
Cells
Dermal fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Skin
Mouse
Mouse, male 6-24 week
Montanaro F, Liadaki K, Volinski J, Flint A. and Kunkel LM.
Skeletal muscle engraftment potential of adult mouse skin side population cells ,
Proc Natl Acad Sci U S A
 100 , 9336 , 2003
Cells
Skin side population
Enzymes
Medium
PBS
Skin
Mouse
Mouse
Takanami-Ohnishi Yoko, Amano Shinya, Kimura Sadao, Asada Sachie, Utani Atsushi, Maruyama Masumi, Osada Hiroyuki, Tsunoda Hajime, Irukayama-Tomobe Yoko, Goto Katsutoshi, Karin Michael, Sudo Tatsuhiko, Kasuya Yoshitoshi.
Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity ,
J Biol Chem
 277 , 37896-903 , 2002
Cells
Ear epidermal
Enzymes
Trypsin : 0.1%
Collagenase : 0.2%
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.