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Tissue References Search Tool

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Tissue

Species

Enzyme(s)

Liver
Rat
Rat
Alston-Smith, J. and Pertoft, H
Isolation of Liver Cells: a System for Obtaining Pure Cells in Monolayer Cultures from a Single Rat Liver , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:14.1 , 1995
Cells
Hepatocytes
Enzymes
Collagenase : 0.04% - 0.06%
Medium
PBS
Liver
Rat
Rat
Matsumoto, K. and Nakamura, T
Techniques for the Isolation and Cultivation of Hepatocytes using Collagenase , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:16.1 , 1995
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
DMEM
Liver
Rat
Rat, SD, male, 200 g
Rana, B. , Mischoulon, D. , Xie, Y. , Bucher, N. and Farmer, S.
Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors ,
Mol Cell Biol
 14 (9) , 5858 , 1994
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Medium 199
Liver
Rat
Rat, SD, male, 250-350 g
Dixit, V. , Arthur, M. and Gitnick, G.
Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats ,
Cell Transplant
 1 , 275 , 1992
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
RPMI 1640
Liver
Rat
Rat, Wistar, adult, male, 100-125 g
Liu, J. , McKim, J. , Liu, Y. and Klaassen, C.
Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures ,
In Vitro Cell Dev Biol
 28 , 320 , 1992
Cells
Hepatocytes
Enzymes
Medium
Serum-free medium
Liver
Rat
Rat, Fisher
Blouin, M. and Marceau, N.
Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells ,
J Tiss Cul Meth
 13 , 117 , 1991
Cells
Epithelial
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.075%
Medium
Eagles's MEM
Liver
Rat
Rat, SD, male, 175-225 g
Reddy, S. , Amick, G. , Cooper, R. and Damun, Z.
Insulin Stimulates the Activity of a Protamine in Isolated Rat Hepatocytes ,
J Biol Chem
 265 , 7748 , 1990
Cells
Hepatocytes
Medium
Kreb's Ringer bicarbonate buffer
Liver
Rat
Rat, SD, 8 - 12 day
Davila, J. , Reddy, C. , Davis, P. and Acosta, D.
Toxicity Assessment of Papaverine Hydrochloride and Papaverine-Derived Metabolites in Primary Cultures of Rat Hepatocytes ,
In Vitro Cell Dev Biol
 26 , 515 , 1990
Cells
Parenchymal hepatocytes
Enzymes
Collagenase Type 4 : 80 u/ml
Medium
HBSS
Liver
Rat
Rat, Wistar, male, 200 g
Kindberg, G. , Gudmundsen, O. and Berg, T.
The Effect of Vandate on Receptor-mediated Endocytosis of Asialoorosomucoid in Rat Liver Parenchymal Cells ,
J Biol Chem
 265 , 8999 , 1990
Cells
Parenchymal
Medium
HEPES, modified
Liver
Rat
Rat, SD, male, 250-350 g
Liu, J. , Kershaw, W. and Klaassen, C.
Rat Primary Hepatocyte Cultures are a Good Model for Examining Metallothionein-Induced Tolerance to Cadmium Toxicity ,
In Vitro Cell Dev Biol
 26 , 75 , 1990
Cells
Hepatocytes
Enzymes
Medium
HBSS, CMF
Liver
Rat
Rat, SD, neonatal, 8 - 10 days
Davila, J. , Lenherr, A. and Acosta, D.
Protective Effect of Flavonoids on Drug-Induced Hepatotoxicity In Vitro ,
Toxicology
 57 , 267 , 1989
Cells
Hepatocytes
Enzymes
Medium
Hanks' BSS, CF
Liver
Rat
Rat, Fisher, male, 125-250 g
Chan, K. , Kost, D. and Michalopoulos, G.
Multiple Sequential Periods of DNA Synthesis and Quiescence in Primary Hepatocyte Cultures Maintained on the DMSO-EGF On/Off Protocol ,
J Cell Physiol
 141 , 584 , 1989
Cells
Hepatocytes
Enzymes
Medium
RPMI 1640
Liver
Rat
Rat, Fischer 344, male, 14 months
Nagy, I. , Ohno-Iwashita, Y. , Ohta, M. , Nagy, V. , Kitani, K. , Ando, S. and Imahori, K.
Effect of Perfringolysin O on the Lateral Diffusion Constant of Membrane Proteins of Hepatocytes as Revealed by Fluorescence Recovery After Photobleaching ,
Biochim Biophys Acta
 939 , 551 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Ringer's biocarbonate buffer
Liver
Rat
Rat, SD, male, 200-300 g
Voss, A. and Sprecher, H.
Metabolism of 6,9,12-Octadecatetraenoic and 6,9,12,15-Octadecatetraenoic Acid ,
Biochim Biophys Acta
 958 , 153 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES
Liver
Rat
Rat, CD strain, albino, male, 140 -180 g
Braun, L. , Mead, J. , Panzica, M. , Mikumo, R. , Bell, G. and Fausto, N.
Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation ,
Proc Natl Acad Sci U S A
 85 , 1539 , 1988
Cells
Hepatocytes and Nonparenchymal
Enzymes
Pronase : 0.1%
Medium
HEPES buffer with calcium
Liver
Rat
Rat, SD, male, 180-200 g
Gupta, C. , Hattori, A. , Betschart, J. , Virji, M. and Shinozuka, H.
Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet ,
Cancer Res
 48 , 1162 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
MEM
Liver
Rat
Rat, Wistar, female, 17 days
Cotariu, D. , Barr-Nea, L. , Papo, N. and Zaidman, J.
Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes ,
Enzyme
 40 , 212 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Wistar, male,3 month, 200 g
Kuiper, J. , Zijlstra, F. , Kamps, J. and vanBerkel, T.
Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells ,
Biochim Biophys Acta
 959 , 143 , 1988
Cells
Parenchymal
Endothelial
Kupffer
Enzymes
Medium
Krebs Henseleit
Liver
Rat
Rat, Wistar, male, 200 - 300 g
Cai, H. , He, Z. and Ding, Y.
Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors ,
Biochim Biophys Acta
 958 , 334 , 1988
Cells
Parenchymal
Kupffer
Enzymes
Medium
HBSS with CaCl2
Liver
Rat
Rat, 250-350 g
(also guinea-pig & rabbit)
Chang, T. and Chang, C.
Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution ,
Biochim Biophys Acta
 942 , 57 , 1988
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 166 u/ml
Medium
DMEM
Liver
Rat
Rat, Fisher 344, 150-200 g
Mathis, G. , Walls, S. and Sirica, A.
Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes ,
Cancer Res
 48 , 6145 , 1988
Cells
Bile ductular epithelial
Enzymes
Collagenase Type 1 : 220 u/ml
Medium
L-15
Liver
Rat
Rat, Wistar, male, 260-310 g
McAbee DD. and Weigel PH
ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes ,
Biochemistry
 27 , 2061 , 1988
Cells
Hepatocytes
Enzymes
Medium
HEPES
Liver
Rat
Rat, SD, male, 350-400 g
Rodriguez de Turco, E. and Spitzer, J.
Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats ,
Biochem J
 253 , 73 , 1988
Cells
Hepatocytes
Medium
HBSS
Liver
Rat
Rat, SD, male, 350 - 450 g
Friedman, S. and Roll, F.
Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan ,
Anal Biochem
 161 , 207 , 1987
Cells
Lipocytes
Kupffer
Sinusoidal endothelial
Enzymes
Collagenase : 0.015%
Medium
DMEM/Ham's F-12
Liver
Rat
Rat, SD, male
Oka, J. and Weigel, P.
Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes ,
J Cell Physiol
 133 , 243 , 1987
Cells
Hepatocytes
Enzymes
Collagenase : 100 - 200 µg/g body weight
Medium
Eagle's
Eagle's w/HEPES
HBSS
Liver
Rat
Rat, SD, male, 200-250 g
Kreamer, B. , Staecker, J. , Sawada, N. , Sattler, G. , Hsia, M. and Pitot, H.
Use of Low-Speed, Iso-Density Percoll Centrifugation Method to Increase the Viability of Isolated Rat Hepatocyte Preparations ,
In Vitro Cell Dev Biol
 22 (4) , 201 , 1986
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Fischer, male, 150-250 g
Francavilla, A. , Ove, P. , Polimeno, L. , Sciascia, C. , Coetzee, M. and Starzi, T.
Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy ,
Cancer Res
 46 , 1318 , 1986
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 125 - 250 u/ml
Medium
MEM
(see reference)
Liver
Rat
Rat, SD, female, 100-180 g
Schwarz, K. , Lanier, S. , Carter, E. , Homcy, C. and Graham, R.
Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture ,
Mol Pharmacol
 27 , 200 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 0.5%
Medium
Krebs Ringer bicarbonate buffer
Liver
Rat
Rat, Lewis, female, 200-250 g
Holstege A, Leser HG, Pausch J, Gerok W.
Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes ,
Eur J Biochem
 149 , 169-73 , 1985
Cells
Hepatocytes, Kupffer, endothelial
Enzymes
Collagenase : 0.05%
Medium
Gey's BSS
Liver
Rat
Rat, Wistar, adult, male, 150-200 g
Okumura, T. and Saito, K.
Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes ,
J. Biochem.
 96 , 429 , 1984
Cells
Hepatocytes
Enzymes
Trypsin : 0.005%
Medium
Williams E
Liver
Rat
Rat, SD, male, 270 - 320 g
Brass, E. , Garrity, M. and Robertson, R.
Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins ,
F.E.B.S. Lett.
 169 , 293 , 1984
Cells
Hepatocytes
Enzymes
Collagenase : 0.04%
Medium
Bicarbonate buffer with calcium added
Liver
Rat
Rat, Wistar, male, 3 month old
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Liver
Rat
Rat, Fischer, Lewis and SD, male 10-18 months
Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.
Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats ,
In Vitro
 19 , 576 , 1983
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS CMF
Liver
Rat
Rat, SD, male/female, 250-300 g
Studer, R. and Borle, A.
Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines ,
J Biol Chem
 257 , 7987 , 1982
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
Krebs Henseleit bicarbonate buffer
Liver
Rat
Rat, Wistar, 12 day
Malan-Shibley, L. and Iype, P.
Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels ,
Exp Cell Res
 131 , 363 , 1981
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS, CMF
Liver
Rat
Rat, SD
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.