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Tissue References Search Tool

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Endothelial
Porcine
Porcine, 6 month
Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
 10 , e0130749 , 2015
Cells
Valvular endothelial
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine
Gould, R. and Butcher, J.
Isolation of Valvular Endothelial Cells. ,
J Vis Exp
 46 , e2158 , 2010
Cells
Valvular endothelial
Enzymes
Collagenase Type 2 : 300-600 u/ml
Medium
DMEM
Endothelial
Porcine
Porcine
(also bovine)
Nugent, H. and Edelman, E.
Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury ,
J Surg Res
 99 , 228 , 2001
Cells
Endothelial
Medium
DMEM w/ 10% calf serum
Endothelial
Porcine
Porcine
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY. and Koh GY.
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells ,
Circulation
 101(19) , 2317 , 2000
Cells
HUVEC, porcine pulmonary arterial endothelial cells
Enzymes
Medium
DMEM
Endothelial
Porcine
Porcine, 6-7 month
Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM. and Tisher CC.
Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV ,
J Am Soc Nephrol
 10(3) , 481 , 1999
Cells
Porcine pulmonary endothelial
Enzymes
Medium
RPMI 1640
Endothelial
Porcine
Porcine
Shasby, S.
Endothelial Cells Grown On Permeable Membrane Supports ,
J Tiss Cul Meth
 14 , 247 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
Medium 199
Endothelial
Porcine
Porcine
Vischer, P. and Buddecke, E.
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells ,
Exp Cell Res
 158 , 15 , 1985
Cells
Endothelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Endothelial
Porcine
Porcine
Dickinson, E. and Slakey, L.
Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta ,
In Vitro
 18 , 63 , 1982
Cells
Endothelial
Aortic
Enzymes
Medium
Dulbecco-Vogt MEM w/o serum
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Porcine
Porcine, 20-30 week
Slater, D.N. and Sloan, J.M.
The Porcine Endothelial Cell in Tissue Culture ,
Atherosclerosis
 21 , 259 , 1975
Cells
Endothelial
Aortas
Veins
Enzymes
Collagenase : 0.1%
Medium
Medium 199 w/BSS and HEPES or NaHCO3
Endothelial
Porcine
Porcine, 60-100 days
Coulson, W.F.
The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin ,
Biochim Biophys Acta
 237 , 378 , 1971
Cells
Aorta
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer
(see reference)
Muscle
Porcine
Porcine, postnatal
Miersch, C. , Stange, K. and Rontgen, M.
Effects of Trypsinization and of a Combined Trypsin, Collagenase, and DNase Digestion on Liberation and in Vitro Function of Satellite Cells Isolated from Juvenile Porcine Muscles. ,
In Vitro Cell Dev Biol Anim
 54 , 406-412 , 2018
Cells
Muscle satellite
Enzymes
Medium
Eagle MEM
Muscle
Porcine
Porcine, 2 month
Lewis, F. , Henning, B. , Marazzi, G. , Sassoon, D. , Ellison, G. and Nadal-Ginard, B.
Porcine Skeletal Muscle-Derived Multipotent PW1pos/Pax7neg Interstitial Cells: Isolation, Characterization, and Long-Term Culture. ,
Stem Cells Transl Med
 3 , 702-12 , 2014
Cells
Skeletal muscle
Enzymes
Medium
HBSS
Muscle
Porcine
Porcine, male, 25-40 kg
Korzick, D. , Laughlin, M. and Bowles, D.
Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries ,
J Appl Physiol
 96 , 1425-32 , 2004
Cells
Coronary myocytes
Enzymes
Medium
low calcium physiological saline
Muscle
Porcine
Porcine, adult, 35-45 kg
Wamhoff BR, Dixon JL, Sturek M.
Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia ,
J Vasc Res
 39 , 208 , 2002
Cells
Arterial smooth muscle
Enzymes
Collagenase Type 2 : 294 u/ml
Elastase : 6.5 u/ml
Deoxyribonuclease I : 0.4 mg/ml
Medium
MEM
Muscle
Porcine
Porcine, Yorkshire, 30kg
Sirous ZN, Fleming JB, Khalil RA.
Endothelin-1 enhances eicosanoids-induced coronary smooth muscle contraction by activating specific protein kinase C isoforms ,
Hypertension
 37 , 497-504 , 2001
Cells
Coronary smooth muscle
Enzymes
Collagenase Type 2 : 150 u/ml
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate
Muscle
Porcine
Porcine, juvenile, 30kg
Huckle WR, Drag MD, Acker WR, Powers M, McFall RC, Holder DJ, Fujita T, Stabilito II, Kim D, Ondeyka DL, Mantlo NB, Chang RS, Reilly CF, Schwartz RS, Greenlee WJ, Johnson RG.
Effects of subtype-selective and balanced angiotensin II receptor antagonists in a porcine coronary artery model of vascular restenosis ,
Circulation
 93 , 1009-19 , 1996
Cells
Coronary smooth muscle cells
Enzymes
Collagenase : 0.3%
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine
Xiong, Yimin, Xu, Shangzhe. and Slakey, Linda L
Modulation of Response to Adenosine in Vascular Smooth Muscle Cells Cultured in Defined Medium ,
In Vitro Cell Dev Biol
 27 , 355 , 1991
Cells
Smooth muscle, aorta
Enzymes
Collagenase : 0.3%
Medium
DMEM
Muscle
Porcine
Porcine
Breton, M. , Berrou, E. , Deudon, E. and Picard, J.
Changes in Proteoglycans of Cultured Pig Aortic Smooth Muscle Cells During Subculture ,
In Vitro Cell Dev Biol
 26 , 157 , 1990
Cells
Smooth muscle
Aorta
Enzymes
Trypsin : 0.05%
Medium
EDTA 0.02%
Muscle
Porcine
Porcine
Fehr, T. , Dickinson, E. , Goldman, S. and Slakey, L.
Cyclic AMP Efflux is Regulated by Occupancy of The Adenosine Receptor in Pig Aortic Smooth Muscle Cells ,
J Biol Chem
 265 , 10974 , 1990
Cells
Smooth muscle
Aortic medial tissue
Enzymes
Collagenase : 0.30%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.