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Tissue References Search Tool

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Endothelial
Porcine
Porcine, 6 month
Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
 10 , e0130749 , 2015
Cells
Valvular endothelial
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine
Gould, R. and Butcher, J.
Isolation of Valvular Endothelial Cells. ,
J Vis Exp
 46 , e2158 , 2010
Cells
Valvular endothelial
Enzymes
Collagenase Type 2 : 300-600 u/ml
Medium
DMEM
Endothelial
Porcine
Porcine
(also bovine)
Nugent, H. and Edelman, E.
Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury ,
J Surg Res
 99 , 228 , 2001
Cells
Endothelial
Medium
DMEM w/ 10% calf serum
Endothelial
Porcine
Porcine
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY. and Koh GY.
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells ,
Circulation
 101(19) , 2317 , 2000
Cells
HUVEC, porcine pulmonary arterial endothelial cells
Enzymes
Medium
DMEM
Endothelial
Porcine
Porcine, 6-7 month
Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM. and Tisher CC.
Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV ,
J Am Soc Nephrol
 10(3) , 481 , 1999
Cells
Porcine pulmonary endothelial
Enzymes
Medium
RPMI 1640
Endothelial
Porcine
Porcine
Shasby, S.
Endothelial Cells Grown On Permeable Membrane Supports ,
J Tiss Cul Meth
 14 , 247 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
Medium 199
Endothelial
Porcine
Porcine
Vischer, P. and Buddecke, E.
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells ,
Exp Cell Res
 158 , 15 , 1985
Cells
Endothelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Endothelial
Porcine
Porcine
Dickinson, E. and Slakey, L.
Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta ,
In Vitro
 18 , 63 , 1982
Cells
Endothelial
Aortic
Enzymes
Medium
Dulbecco-Vogt MEM w/o serum
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Porcine
Porcine, 20-30 week
Slater, D.N. and Sloan, J.M.
The Porcine Endothelial Cell in Tissue Culture ,
Atherosclerosis
 21 , 259 , 1975
Cells
Endothelial
Aortas
Veins
Enzymes
Collagenase : 0.1%
Medium
Medium 199 w/BSS and HEPES or NaHCO3
Endothelial
Porcine
Porcine, 60-100 days
Coulson, W.F.
The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin ,
Biochim Biophys Acta
 237 , 378 , 1971
Cells
Aorta
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer
(see reference)
Pancreas
Porcine
Porcine, juvenile
Van der Burg Michael P M, Graham John M.
Iodixanol Density Gradient Preparation in University of Wisconsin solution for porcine islet purification ,
ScientificWorldJournal
 3 , 1154-9 , 2003
Cells
Islets
Enzymes
Collagenase : see reference
Medium
Univ of Wisconsin solution
Pancreas
Porcine
Porcine, 3 month, 15-20 kg
Zhao, X. , Han, J. and Tang, C.
Primary Culture of Porcine Pancreatic Acinar Cells ,
JOP
 2 (2) , 78 , 2001
Cells
Acinar
Enzymes
Collagenase Type 3 : 200 u/ml
Medium
RPMI 1640
Pancreas
Porcine
Porcine, 1-3 year, 1.5-2.0 kg, either sex
Korbutt, G. , Elliott, J. , Ao, Z. , Smith, D. , Warnock, G. and Rajotte, R.
Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells ,
J Clin Invest
 97 (9) , 2119 , 1996
Cells
Islets
Enzymes
Collagenase : 0.25%
Medium
HBSS
Pancreas
Porcine
Porcine, female
Brandhorst, D. , Brandhorst, H. , Hering, B. , Federlin, K. and Bretzel, R.
Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience ,
Exp Clin Endocrinol
 103 , 3 , 1995
Cells
Islets
Enzymes
Collagenase : 0.1%
Medium
HBSS
Pancreas
Porcine
Porcine
Johnson, P. , van Suylichem, P. , Roberts, D. , Vos-Scheperkeuter, G. , White, S. , van Schlifgaarde, R. and London, N.
Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation ,
Xenotransplantation
 2 , 165 , 1995
Cells
Islets
Enzymes
Collagenase : 0.1%-0.2%
Medium
HBSS
Pancreas
Porcine
Porcine, <2 year, 200-250 kg
Heiser, A. , Ulrichs, K. and Muller-Ruchholtz, W.
Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields ,
J Clin Lab Anal
 8 , 407 , 1994
Cells
Islets
Enzymes
Collagenase : 0.1%
Medium
HBSS
Pancreas
Porcine
Porcine, 10-36 months, 200-300 kg
Ricordi, C. , Socci, C. , Davalli, A. , Staudacher, C. , Baro, P. , Vertova, A. , Sassi, I. , Gavazzi, F. , Pozza, G. and Di Carlo, V.
Isolation of the Elusive Pig Islet ,
Surgery
 107 (6) , 688 , 1990
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
HBSS
Pancreas
Porcine
Porcine
Iwatsuki, N. and Peterson, O.
Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording ,
J Membr Biol
 86 , 139 , 1985
Cells
Acinar
Enzymes
Collagenase : 100 u/ml
Medium
Saline

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.