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Tissue References Search Tool

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Reproductive
Rat
Rat, SD, immature
Ando, M. , Kol, S. , Irahara, M. , Sirois, J. and Adashi, E.
Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process ,
Mol Cell Endocrinol
 157 , 21 , 1999
Cells
Ovary
Enzymes
Collagenase Type 1 : 144 u/ml
Medium
McCoy's 5a
Reproductive
Rat
Rat, Han-Wistar, adult, male, 250 g
Leckie, C. , Welberg, L. and Seckl, J.
11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells ,
J Endocrinol
 159 , 233 , 1998
Cells
Testes
Enzymes
Trypsin : 0.1%
Medium
Medium 199 w/ Hank's salts
Reproductive
Rat
Rat, SD, female, immature, 26 day
(also rat, pregnant and pseudopregnant)
Tellieria, C. , Ou, J. , Sugino, N. , Ferguson, S. and Gibori, G.
The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid ,
Endocrinology
 139 (8) , 3597 , 1998
Cells
Corpus luteum
Enzymes
Neutral Protease : 2.4 u/ml Deoxyribonuclease: 200u/ml
Medium
Serum-free medium
(see reference)
Reproductive
Rat
Rat, SD, 25 day
Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR.
Effects of insulin and insulin-like growth factors on proliferation of rat ovarian theca-interstitial cells ,
Biol Reprod
 56 , 891-7 , 1997
Cells
Ovarian theca-interstitial
Enzymes
Medium
Medium 199
Reproductive
Rat
Rat, SD, male, 90 days
Onoda, M. and Djakiew, D.
Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin ,
In Vitro Cell Dev Biol
 27 , 215 , 1991
Cells
Sertoli
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM
Reproductive
Rat
Rat, SD, male
Abayasekara, D. , Kurlak, L. , Band, A. , Sullivan, M. and Cooke, B.
Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis ,
In Vitro Cell Dev Biol
 27 , 253 , 1991
Cells
Leydig
Enzymes
Trypsin : 0.02%
Medium
DMEM
Reproductive
Rat
Rat, neonatal
Branham, W. , Lyn-Cook, B. , Andrews, A. , McDaniel, M. and Sheehan, D.
Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix ,
In Vitro Cell Dev Biol
 27 , 442 , 1991
Cells
Uterine
Enzymes
Trypsin : 1%
Medium
HBSS
Reproductive
Rat
Rat, Wistar, adult
Pampfer, S. , Vanderheyden, I. , Michiels, B. and DeHertogh, R.
Co-Culture of Two-Cell Rat Embryos on Cell Monolayers ,
In Vitro Cell Dev Biol
 26 , 944 , 1990
Cells
Uterine
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, 10 day
Hadley MA, Weeks BS, Kleinman HK, Dym M.
Laminin promotes formation of cord-like structures by Sertoli cells in vitro ,
Dev Biol
 140 , 318-27 , 1990
Cells
Sertoli cells
Enzymes
Medium
PBS
Reproductive
Rat
Rat, SD, male, 120-160 g
Ng, T. and Liu, W.
Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis ,
In Vitro Cell Dev Biol
 26 , 24 , 1990
Cells
Leydig
Adrenal
Enzymes
Collagenase Type 2 : 0.03% (adrenal)
Medium
Krebs Ringer bicarbonate buffer
Reproductive
Rat
Rat, immature
Rajan, V. and Menon, K.
Differential Uptake and Metabolism of Free and Esterified Cholesterol from High-density Lipoproteins in the Ovary ,
Biochim Biophys Acta
 959 , 206 , 1988
Cells
Luteal, ovaries
Enzymes
Collagenase : 0.3%
Medium
McCoy's
Reproductive
Rat
Rat, SD, female
Glasser, S. , Julian, J. , Decker, G. , Tang, J. and Carson, D.
Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells ,
J Cell Biol
 107 (6) , 2409 , 1988
Cells
Luminal epithelial
Enzymes
Trypsin : 0.5%
Medium
HBSS
Reproductive
Rat
Rat, male, 20 day
Skinner, M. , Fetterolf, P. and Anthony, C.
Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function ,
J Biol Chem
 263 , 2884 , 1988
Cells
Sertoli
Enzymes
Trypsin : 0.15%
Medium
(see reference)
Reproductive
Rat
Rat, Wistar, virgin, female, 250 g
Conti, C. and Tasat, D.
Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone ,
J Steroid Biochem
 24 (3) , 747 , 1986
Cells
Vaginal epithelial
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, female, 21 day
Rajan, V. and Menon, K.
Involvement of Microtubules in Lipoprotein Degradation and Utilization for Steroidogenesis in Cultured Rat Luteal Cells ,
Endocrinology
 117 , 2408 , 1985
Cells
Luteal, ovaries
Enzymes
Medium
McCoy's
Reproductive
Rat
Rat, SD, male, 10 day
Hadley, M. , Byers, S. , Suarez-Quian, C. , Kleinman, H. and Dym, M.
Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro ,
J Cell Biol
 101 , 1511 , 1985
Cells
Sertoli
Enzymes
Trypsin : 0.025%
Medium
DMEM
Reproductive
Rat
Rat, Holtzman, female, immature
Kassis, J. , Walent, J. and Gorski, J.
Estrogen Receptors in Rat Uterine Cell Cultures: Effects of Medium on Receptor Concentration ,
Endocrinology
 115 , 762 , 1984
Cells
Uterine
Enzymes
Medium
DMEM buffered with HEPES
Reproductive
Rat
Rat, SD, adult,male, 50-70 days
Hsueh, A. , Bambino, T. , Zhuang, L. , Welsh, T. and Ling, N.
Mechanism of the Direct Action of Gonadotropin Releasing Hormone and Its Antagonist on Androgen Biosynthesis by Cultured Rat Testicular Cells ,
Endocrinology
 112 , 1653 , 1983
Cells
Testicular
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
HEPES
Reproductive
Rat
Rat, SD, female, pseudopregnant, 21 day
Rajendran, K. , Hwang, J. and Menon, K.
Binding, Degradation an Utilization of Plasma High Density and Low Density Lipoproteins for Progesterone Production In Cultured Rat Luteal Cells ,
Endocrinology
 112 , 1746 , 1983
Cells
Luteal, ovaries
Enzymes
Hyaluronidase : 0.1%
Medium
EBSS
Reproductive
Rat
Rat, SD, 4-6 day, male
Rich, K. , Bardin, C. , Gunsalus, G. and Mather, J.
Age-Dependent Pattern of Androgen-Binding Protein Secretion from Rat Sertoli Cells in Primary Culture ,
Endocrinology
 113 , 2284 , 1983
Cells
Sertolli, seminiforous tubules
Enzymes
Collagenase : 0.03%
Medium
Serum-free medium
Reproductive
Rat
Rat, SD, female, 26 days
Azhar, S. and Reaven, E.
Effect of Antimicrotubule Agents on Microtubules and Steroidogenesis in Luteal Cells ,
Am J Physiol
 243 , E380 , 1982
Cells
Luteal
Enzymes
Medium
Medium 199
Reproductive
Rat
Rat, SD, male
Ramachandran, J. and Sairam, M.R.
The Effects Of Interstitial Cell-Stimulating Hormone, Its Subunits,and Recombinants on Isolated Rat Leydig Cells ,
Arch Biochem Biophys
 167 , 294 , 1975
Cells
Leydig
Enzymes
Collagenase : 0.1%
Medium
Krebs Ringer bicarbonate buffer
Skin
Rat
Rat, Wistar, 4-8 day
Sugihara, H. and Toda, S
Primary Tissue Intact and Dissociated Cell Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 3A:2.1 , 1995
Cells
Dermal fibroblasts and keratinocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Skin
Rat
Rat, SD, male, 40-60 days
Laurent, S. , Mednieks, M. and Rosenfield, R.
Growth of Sebaceous Cells in Monolayer Culture ,
In Vitro Cell Dev Biol
 28 , 83 , 1992
Cells
Sebaceous
Enzymes
Trypsin : 0.2%
Medium
DMEM with FBS
PBS
Skin
Rat
Rat, neonatal, 12-24 hr post partum
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
 7 , 265 , 1991
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
HEPES buffered DMEM
Skin
Rat
Rat, albino, one day old, CFN
Vaughan, F. , Gray, R. and Bernstein, I.
Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes ,
In Vitro Cell Dev Biol
 22 (3) , 141 , 1986
Cells
Keratinocytes
Enzymes
Trypsin : 1%
Medium
EBSS
Stem
Rat
Rat, 9 week
Ohta, Y. , Hamaguchi, A. , Ootaki, M. , Watanabe, M. , Takeba, Y. , Iiri, T. , Matsumoto, N. and Takenaga, M.
Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury. ,
Cytotherapy
 19 , 839-848 , 2017
Cells
Adipose stromal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Stem
Rat
Rat
Jiang, H. , Zhang, Y. , Tian, K. , Wang, B. and Han, S.
Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells. ,
Sci Rep
 7 , 41837 , 2017
Cells
Placental mesenchymal
Enzymes
Medium
DMEM
Stem
Rat
Rat, SD, 2-3 month
Dayer, D. , Tabar, M. , Moghimipour, E. , Tabandeh, M. , Ghadiri, A. , Bakhshi, E. , Orazizadeh, M. and Ghafari, M.
Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells ,
Cytotherapy
 19 , 937-946 , 2017
Cells
Mesenchymal stromal
Enzymes
Medium
DMEM
Stem
Rat
Rat, 6-8 week
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Rat
Rat
Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G. and Karaoz, E
Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model. ,
Cytotherapy
 17 , 543-59 , 2015
Cells
Mesenchymal stem
Enzymes
Medium
DMEM
Stem
Rat
Rat. SD, 5 month
Veronesi, F, Torricelli, P, Della, B, Pagani, S. and Fini, M
In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 215-23 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
DMEM
Stem
Rat
Rat, adult
Duarte, S. , Momier, D. , Baque, P. , Casanova, V. , Loubat, A. , Samson, M. , Guigonis, J. , Staccini, P. , Saint-Paul, M. , De Lima, M. , Carle, G. and Pierrefite-Carle, V.
Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model. ,
Stem Cells
 31 , 423-32 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Stem
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Stem
Rat
Rat
Wei, X. , Du, Z. , Zhao, L. , Feng, D. , Wei, G. , He, Y. , Tan, J. , Lee, W. , Hampel, H. , Dodel, R. , Johnstone, B. , March, K. , Farlow, M. and Du, Y.
IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats. ,
Stem Cells
 27 , 478 , 2009
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Stem
Rat
Rat, SD, newborn
Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian.
Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord ,
Dev Neurosci
 27 , 20-6 , 2005
Cells
Neutral stem cells, Schwann cells
Enzymes
Trypsin : 0.25%
Collagenase : 0.16%
Medium
DMEM/F12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.