Tissue References Search Tool

Brain

Rat

Rat, SD, newborn

Pixley, S.K.

The Olfactory Nerve Contains Two Populations of Glia ,
In Vitro
5 , 269 , 1992

Cells

Glial

Enzymes

Trypsin : 0.0625%

Medium

MEM, sterile

Brain

Rat

Rat, 60-72 hours old

Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.

Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
7 , 265 , 1991

Cells

Fibroblasts

Enzymes

Trypsin : 0.2%

Medium

HEPES buffered DMEM

Brain

Rat

Rat, SD, adult

Doron, D. , Jacobowitz, D. , Heldman, E. , Feurerstein, G. , Pollard, H. and Hallenbeck, J.

Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels ,
In Vitro Cell Dev Biol
27A , 689 , 1991

Cells

Endothelial

Enzymes

Collagenase Type 2 : 0.5%

Medium

Medium 199

Brain

Rat

Rat, SD, 5 day

Mattson, M. and Kater, S.

Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
490 , 110 , 1989

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.2%

Deoxyribonuclease I : 10 ug/ml

Medium

HBSS

Brain

Rat

Rat, SD, female

Matsuda, M.

Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture ,
In Vitro Cell Dev Biol
24 (10) , 1031 , 1988

Cells

Fetal rat brain

Enzymes

Collagenase Type 4 : 0.1%

Medium

DMEM

Brain

Rat

Rat, albino, adult and newborn

Giulian, D. and Baker, T.J.

Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain ,
J Neurosci
6 , 2163 , 1986

Cells

Cerebral cortices

Enzymes

Trypsin : 0.25%

Medium

PBS

Brain

Rat

Rat, Wistar, newborn

Goldman, J.E. , Geier, S.S. and Hirano, M.

Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture ,
J Neurosci
6 , 52 , 1986

Cells

Germinal matrix

Enzymes

Trypsin : 0.25%

Medium

HBSS

Brain

Rat

Rat, fetus, 18 day

Rothman, S.

Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death ,
J Neurosci
4 (7) , 1884 , 1984

Cells

Hippocampi

Enzymes

Trypsin : 0.1%

Medium

HBSS

Brain

Rat

Rat, fetus, 18-20 day

Bartlett, W. and Banker, G.

An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
4 , 1944 , 1984

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.25%

Medium

HBSS, CMF

Brain

Rat

Rat, SD, 19-20 days pregnant

Ahmed, Z. , Walker, P. and Fellows, R.

Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture ,
J Neurosci
3 (12) , 2448 , 1983

Cells

Neural

Enzymes

Trypsin : 0.25%

Medium

DMEM

Brain

Rat

Rat, SD, fetal

Peterfreund, R. and Vale, W.

High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell ,
Brain Res
239 , 463 , 1982

Cells

Cerebral cortex

Hypothalmus

Enzymes

Deoxyribonuclease I : 0.001%

Medium

HEPES

Brain

Rat

Rat, fetus

Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.

A Method for Large-scale Production of Mouse Brain Cortical Cultures ,
Brain Res
255 , 361 , 1982

Cells

Cortical

Enzymes

Trypsin : 0.25%

Medium

Puck's D1 (see reference)

Brain

Rat

Rat, Wistar-Kyoto, male, 100 - 200 g

Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J.

Primary Culture of Rat Cerebral Microvascular Endothelial Cells ,
Lab Invest
46 , 554 , 1982

Cells

Endothelial

Cerebral

Enzymes

Collagenase Type 2 : 0.05%

Medium

HBSS

Brain

Rat

Rat, Wistar, pregnant

Abney, E. , Bartlett, P. and Raff, M.

Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain ,
Dev Biol
83 , 301 , 1981

Cells

Glial

Enzymes

Trypsin : 0.05%

Medium

Eagle's MEM/DMEM

Brain

Rat

Rat, SD, one month old

Bowman, P. , Betz, A. , Ar, D. , Wolinsky, J. , Penney, J. , Shivers, R. and Goldstein, G.

Primary Culture of Capillary Endothelium From Rat Brain ,
In Vitro
17 (4) , 353 , 1981

Cells

Capillary endothelium

Pericytes

Enzymes

Neutral Protease : 0.1%

Medium

Medium 199

Brain

Rat

Rat, Wistar, male, 300-500 g

Williams, S. , Gillis, J. , Matthews, M. , Wagner, R. and Bitensky, M.

Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity ,
J Neurochem
35 (2) , 374 , 1980

Cells

Microvessels

Endothelial

Enzymes

Collagenase Type 2 : 0.75%

Medium

Ringers-HEPES buffer

Brain

Rat

Rat, Wistar, adult, 170 g

Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.

Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
129 , 261 , 1979

Cells

Endothelial

Enzymes

Trypsin : 0.5%

Medium

BSS

Brain

Rat

Rat, Holtzmann, 18 day

Banker, G. and Cowan, M.

Rat Hippocampal Neurons in Dispersed Cell Culture ,
Brain Res
126 , 397 , 1977

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.1%

Medium

HBSS

Brain

Rat

Rat, SD, 2 days

Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B.

Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons ,
Exp Cell Res
104 , 345 , 1977

Cells

Pineal

Enzymes

Trypsin : 0.25%

Medium

DMEM, MEM

Brain

Shellfish

Helisoma trivolvis

Hadley, R.D. , Bodnar, D.A. and Kater, S.B.

Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation ,
J Neurosci
5 , 3145 , 1985

Cells

Neurons

Buccal ganglia

Enzymes

Trypsin : 0.2%

Medium

Saline, Sterile

Cartilage

Rat

Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.

Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
12 , e0176107 , 2017

Cells

Chondrocytes

Enzymes

Collagenase Type 2 : 0.15%

Trypsin : 0.25%

Medium

DMEM/F12

Cartilage

Rat

Rat, SD, young, 100-120 g

Shimomura, Y. , Yoneda, T. and Suzuki, F.

Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
19 , 179 , 1975

Cells

Chondrocytes

Enzymes

Trypsin : 0.2%

Medium

Ham's F-12 medium

Colon

Rat

Rat, adult

Somara, S. , Bashllari, D. , Gilmont, R. and Bitar, K.

Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA. ,
Am J Physiol Gastrointest Liver Physiol
300 , G1022 , 2011

Cells

Colon smooth muscle

Enzymes

Soybean Trypsin Inhibitor : 0.01%

Collagenase Type 2 : 0.1%

Medium

DMEM

Endothelial

Rat

Rat, SD, male, 250-300 g

Schwertschlag, U.S. and Whorton, A.R.

Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
263 , 13791 , 1988

Cells

Smooth muscle, aorta

Enzymes

Soybean Trypsin Inhibitor : 0.25%

Medium

HBSS with 0.2 mM Ca++

Endothelial

Rat

Rat, SD, male, 350 - 450 g

Friedman, S. and Roll, F.

Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan ,
Anal Biochem
161 , 207 , 1987

Cells

Lipocytes

Kupffer

Sinusoidal endothelial

Enzymes

Collagenase : 0.015%

Medium

DMEM/Ham's F-12

Endothelial

Rat

Rat, Wistar, male, 3 mo

Nagelkenke, J. , Barto, K. and VanBerkel, T.

In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
258 , 12221 , 1983

Cells

Endothelial

Kupffer

Parenchymal

Enzymes

Collagenase : 0.05%

Pronase : 0.25%

Medium

HBSS

Endothelial

Rat

Rat, Wistar-Kyoto, male, 100 - 200 g

Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J.

Primary Culture of Rat Cerebral Microvascular Endothelial Cells ,
Lab Invest
46 , 554 , 1982

Cells

Endothelial

Cerebral

Enzymes

Collagenase Type 2 : 0.05%

Medium

HBSS

Endothelial

Rat

Rat, 300 G, and pig, 30-40 Kg

Merrilees, M.J. and Scott, L.

Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
39 , 147 , 1981

Cells

Endothelial

Thoracic aorta

Enzymes

Collagenase Type 4 : 0.025%

Trypsin : 0.05%

Medium

Medium 199 and 0.01M EDTA

Endothelial

Rat

Folkman, J. , Haudenschild, C. C. and Zetter, B. R.

Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
76 , 5217 , 1979

Cells

Foreskin

Enzymes

Collagenase : 0.5%

Medium

Dulbecco's MEM w/10% calf serum

Endothelial

Rat

Rat, Wistar, adult, 170 g

Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.

Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
129 , 261 , 1979

Cells

Endothelial

Enzymes

Trypsin : 0.5%

Medium

BSS

Epithelial

Rat

Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.

The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
115 , 412 , 2010

Cells

Mammary epithelial

Enzymes

Collagenase Type 3 : 0.35%

Medium

HBSS

Epithelial

Rat

Rat, SD, adult, male, 8-10 weeks old

Abou-Haila, A. and Tulsiani, D.

Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
3 (1) , 35 , 2001

Cells

Seminiferous tubules

Enzymes

Trypsin : 0.05%

Medium

Krebs-Ringer bicarbonate buffer

(see reference)

Epithelial

Rat

Rat, male

Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.

Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
112 , 243 , 1999

Cells

Alveolar epithelial

Enzymes

Elastase : 40 u/ml

Medium

DMEM

Epithelial

Rat

Rat, embryonic

Shannon JM, Gebb SA. and Nielsen LD

Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
126 , 1675 , 1999

Cells

Tracheal epithelial

Enzymes

Collagenase Type 4 : 0.05%

Medium

DMEM/F12

Epithelial

Rat

Rat, fetal, 14-15 day

Simon-Assmann, P. and Kedinger, M

Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995

Cells

Intestinal mesenchymal and epithelial

Enzymes

Collagenase : 0.03%

Medium

DMEM

Epithelial

Rat

Rat, 4-14 week

Wang N, Koutz CA. and Anderson RE.

A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
34(1) , 101 , 1993

Cells