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Tissue References Search Tool

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Epithelial
Rat
Rat
Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.
The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
 115 , 412 , 2010
Cells
Mammary epithelial
Enzymes
Medium
HBSS
Epithelial
Rat
Rat, SD, adult, male, 8-10 weeks old
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Epithelial
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, fetal, 14-15 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Epithelial
Rat
Rat, 6 day old
Evans GS, Flint N, Somers AS, Eyden B. and Potten CS.
The development of a method for the preparation of rat intestinal epithelial cell primary cultures ,
J Cell Sci
 101 , 219 , 1992
Cells
Rat intestinal epithelial
Enzymes
Collagenase : 300 u/ml
Medium
DMEM
Epithelial
Rat
Rat, SD, adult, 90-120 day old, 350-450 g
Klinefelter, G.
A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats ,
J Tiss Cul Meth
 14 , 195 , 1992
Cells
Epididymal epithelial
Enzymes
Medium
HBSS
Epithelial
Rat
Rat, adult
(also hamster)
Heimann, T. and Githens, S.
Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix ,
Pancreas
 6 (5) , 514 , 1991
Cells
Interlobular duct fragments
Medium
DMEM/Ham's F-12
Epithelial
Rat
Rat, SD, 200 g
Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S.
Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro ,
Peptides
 12 , 63 , 1991
Cells
Colon
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
(see reference)
Epithelial
Rat
Rat, SD, male, 150 - 250 g
Dial, E. , Kao, Y. and Lichtenberger, L.
Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture ,
In Vitro Cell Dev Biol
 27 , 39 , 1991
Cells
Epithelial
Stomach
Enzymes
Pronase : 0.15%
Medium
Medium 199
Epithelial
Rat
Rodent, various (see reference)
Robinson, C. and Wu, R.
Culture of Conducting Airway Epithelial Cells in Serum-Free Medium ,
J Tiss Cul Meth
 13 , 95 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.2%
Medium
MEM, PBS
Epithelial
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Epithelial
Rat
Rat, Wistar, neonatal
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS
Epithelial
Rat
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat (ACI/MNs) male, 8 weeks
Masuda, A. , Ohtsuka, K. and Matsuyama, M.
Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
 26 , 713 , 1990
Cells
Epthelial
Enzymes
Medium
Eagle's MEM Serum-free
Epithelial
Rat
Rat, Fisher 344, male, 8 wks old,
250-300 g
Chang, L. , Wu, R. and Nettesheim, P.
Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture ,
J Cell Sci
 74 , 283 , 1985
Cells
Tracheal epithelial
Enzymes
Pronase : 0.5%
Medium
DMEM
Epithelial
Rat
Rat, Fischer, male, 4-6 weeks
Babcock, M. , Marino, M. , Gunning, W. and Stoner, G.
Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells ,
In Vitro
 19 , 403 , 1983
Cells
Epithelial
Esophagus
Enzymes
Hyaluronidase : 0.1%
Medium
HEPES BSS
Epithelial
Rat
Rat, Fischer, Lewis and SD, male, 10-18 months
Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.
Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats ,
In Vitro
 19 , 576 , 1983
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS CMF
Epithelial
Rat
Rat, SD, female
Cohen, L.
Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
 18 , 565 , 1982
Cells
Epithelial, cancer and tumor
Enzymes
Collagenase : 0.1%
Medium
Eagles's MEM
Epithelial
Rat
Rat, Wistar, 12 day
Malan-Shibley, L. and Iype, P.
Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels ,
Exp Cell Res
 131 , 363 , 1981
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS, CMF
Epithelial
Rat
Rat, Fischer, adult, 200-250 g
Williams, G. and Gunn, J.
Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
 89 , 139 , 1974
Cells
Epithelial
Enzymes
Hyaluronidase : 0.0075%
Medium
KCl-NaCl HEPES Buffer
Epithelial
Rat
Rat, Fischer, 10 day
Williams, G. , Weisburger, E. and Weisburger, J.
Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver ,
Exp Cell Res
 69 , 106 , 1971
Cells
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
PBS
Reproductive
Rat
Rat
Jiang, H. , Zhang, Y. , Tian, K. , Wang, B. and Han, S.
Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells. ,
Sci Rep
 7 , 41837 , 2017
Cells
Placental mesenchymal
Enzymes
Medium
DMEM
Reproductive
Rat
Rat, SD, 350-450 g
Sharma RS, Pal PC, Rajalakshmi M.
Isolation and Culture of Leydig Cells from Adult Rats ,
Indian J Clinical Biochem
 21 , 27 , 2006
Cells
Leydig cells
Enzymes
Collagenase : .05-0.1%
Medium
Medium 199
Reproductive
Rat
Rat, SD, adult, male, 8 - 10 week
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Reproductive
Rat
Rat, SD, immature
Ando, M. , Kol, S. , Irahara, M. , Sirois, J. and Adashi, E.
Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process ,
Mol Cell Endocrinol
 157 , 21 , 1999
Cells
Ovary
Enzymes
Collagenase Type 1 : 144 u/ml
Medium
McCoy's 5a
Reproductive
Rat
Rat, Han-Wistar, adult, male, 250 g
Leckie, C. , Welberg, L. and Seckl, J.
11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells ,
J Endocrinol
 159 , 233 , 1998
Cells
Testes
Enzymes
Trypsin : 0.1%
Medium
Medium 199 w/ Hank's salts
Reproductive
Rat
Rat, SD, female, immature, 26 day
(also rat, pregnant and pseudopregnant)
Tellieria, C. , Ou, J. , Sugino, N. , Ferguson, S. and Gibori, G.
The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid ,
Endocrinology
 139 (8) , 3597 , 1998
Cells
Corpus luteum
Enzymes
Neutral Protease : 2.4 u/ml Deoxyribonuclease: 200u/ml
Medium
Serum-free medium
(see reference)
Reproductive
Rat
Rat, SD, 25 day
Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR.
Effects of insulin and insulin-like growth factors on proliferation of rat ovarian theca-interstitial cells ,
Biol Reprod
 56 , 891-7 , 1997
Cells
Ovarian theca-interstitial
Enzymes
Medium
Medium 199
Reproductive
Rat
Rat, SD, male, 90 days
Onoda, M. and Djakiew, D.
Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin ,
In Vitro Cell Dev Biol
 27 , 215 , 1991
Cells
Sertoli
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM
Reproductive
Rat
Rat, SD, male
Abayasekara, D. , Kurlak, L. , Band, A. , Sullivan, M. and Cooke, B.
Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis ,
In Vitro Cell Dev Biol
 27 , 253 , 1991
Cells
Leydig
Enzymes
Trypsin : 0.02%
Medium
DMEM
Reproductive
Rat
Rat, neonatal
Branham, W. , Lyn-Cook, B. , Andrews, A. , McDaniel, M. and Sheehan, D.
Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix ,
In Vitro Cell Dev Biol
 27 , 442 , 1991
Cells
Uterine
Enzymes
Trypsin : 1%
Medium
HBSS
Reproductive
Rat
Rat, Wistar, adult
Pampfer, S. , Vanderheyden, I. , Michiels, B. and DeHertogh, R.
Co-Culture of Two-Cell Rat Embryos on Cell Monolayers ,
In Vitro Cell Dev Biol
 26 , 944 , 1990
Cells
Uterine
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, 10 day
Hadley MA, Weeks BS, Kleinman HK, Dym M.
Laminin promotes formation of cord-like structures by Sertoli cells in vitro ,
Dev Biol
 140 , 318-27 , 1990
Cells
Sertoli cells
Enzymes
Medium
PBS
Reproductive
Rat
Rat, SD, male, 120-160 g
Ng, T. and Liu, W.
Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis ,
In Vitro Cell Dev Biol
 26 , 24 , 1990
Cells
Leydig
Adrenal
Enzymes
Collagenase Type 2 : 0.03% (adrenal)
Medium
Krebs Ringer bicarbonate buffer
Reproductive
Rat
Rat, immature
Rajan, V. and Menon, K.
Differential Uptake and Metabolism of Free and Esterified Cholesterol from High-density Lipoproteins in the Ovary ,
Biochim Biophys Acta
 959 , 206 , 1988
Cells
Luteal, ovaries
Enzymes
Collagenase : 0.3%
Medium
McCoy's

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.