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Tissue References Search Tool

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Brain
Mouse
Mouse, embryonic and postnatal
Hilgenberg, L. and Smith, M.
Preparation of Dissociated Mouse Cortical Neuron Cultures. ,
J Vis Exp
 10 , 562 , 2007
Cells
Cortical neurons
Enzymes
Papain : 4-10 u/ml
Medium
Neurobasal
Brain
Mouse
Mouse, 1 day
Seaberg, R. , Smukler, S. and Van der Kooy, D.
Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain. ,
Dev Biol
 278 , 71 , 2005
Cells
Neural progenitor
Enzymes
PDS kit : per instructions
Medium
see reference
Brain
Mouse
Mouse
Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R.
Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. ,
Development
 132 , 2425 , 2005
Cells
Granule cell precursors, pre-neoplastic and tumor cells
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal/B27
Brain
Mouse
Mouse
Klein C, Butt SJ, Machold RP, Johnson JE. and Fishell G.
Cerebellum- and forebrain-derived stem cells possess intrinsic regional character ,
Development
 132 , 4497 , 2005
Cells
Neurosperes
Enzymes
PDS kit : per instructions
Medium
DMEM/F12
Brain
Mouse
Mouse
Hernandez, F. , Perez, M. , Lucas, J. , Mata, A. , Bhat, R. and Avila, J.
Glycogen Synthase Kinase-3 plays a Crucial Role in Tau Exon 10 Splicing and Intranuclear Distribution of SC35. Implications for Alzheimer's Disease. ,
J Biol Chem
 279 , 3801 , 2004
Cells
Cortical neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Brain
Mouse
Mouse, 2-5 day
Smeyne Michelle, Smeyne RichardJ.
Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease ,
Brain Res Brain Res Protoc
 9 , 105-11 , 2002
Cells
Postnal substantia nigra
Enzymes
PDS kit : per instructions
Medium
see reference
Brain
Mouse
Mouse, embryonic
Estivill-Torrus, G. , Pearson, H. , Van Heyningen, V. , Price, D. and Rashbass, P.
Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors. ,
Development
 129 , 455 , 2002
Cells
Cortical progenitors
Enzymes
PDS kit : per instructions
Medium
Serum free medium
Brain
Mouse
Mouse, 3 day
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H.
Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia ,
J Biol Chem
 277 , 4816 , 2002
Cells
Microglia
Enzymes
Papain : 90 u/ml
Deoxyribonuclease I : 2000 u/ml
Medium
Eagle's MEM
Brain
Mouse
Mouse, SD, 8-12 week
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL.
IGF-I and microglia/macrophage proliferation in the ischemic mouse brain ,
Glia
 39 , 85 , 2002
Cells
Microglia
Enzymes
Medium
RPMI-1640
Brain
Mouse
Mouse, 1 day
Martin-Aparicio, E. , Yamamoto, A. , Hernandez, F. , Hen, R. , Avila, J. and Lucas, J.
Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease. ,
J Neurosci
 21 , 8772 , 2001
Cells
Neurons and glia
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Brain
Mouse
Mouse
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S. and Ishizaki Y.
A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation ,
J Neurosci
 20 , 5756 , 2000
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 0.435%
Medium
EBSS
Brain
Mouse
Mouse
Lim, D. and Alvarez-Buylla, A.
Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis ,
Proc Natl Acad Sci U S A
 96 , 7526 , 1999
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Brain
Mouse
Mouse, newborn
Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS.
Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice ,
Learn Mem
 5 , 317-30 , 1998
Cells
Hippocampal cells
Enzymes
Papain : 10 u/ml
Medium
DMKM
Brain
Mouse
Mouse, SWR or CF1, 1-3 months
Spielman, A. , Mody, I. , Brand, J. , Whitney, G. , MacDonald, J. and Salter, M.
A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells ,
Brain Res
 503 , 326 , 1989
Cells
Papillae, taste receptor
Enzymes
Pronase E : 0.15%
Medium
Carbonate-Phosphate buffer
(see reference)
Brain
Mouse
Mouse, 0-30 day
Shrier, B. , Wilson, S. and Nirenberg, M.
Cultured Cell Systems and Methods for Neurobiology , Vol. 32 , 765 , 1974
Cells
Neural
Enzymes
Medium
BSS
Brain
Ovine
Ovine
Reddy, R. , Amodei, R. , Estill, C. , Stormshak, F. , Meaker, M. and Roselli, C.
Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. ,
PLoS ONE
 10 , e0129521 , 2015
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Ovine
Ovine
Lepore G, Gadau S, Mura A, Zedda M, Farina V.
Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury. ,
Eur J Histochem
 53 , e28 , 2009
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Brain
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Brain
Porcine
Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months
Robinson, D.H. , Kang,Y. , Deschner, S.H. and Nielsen, T.B.
Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture ,
In Vitro Cell Dev Biol
 26 , 169 , 1990
Cells
Microvascular
Enzymes
Collagenase : 0.1%
Medium
HBSS
Brain
Rat
Rat, SD, 1-2 day
Kurachi, M. , Mikuni, M. and Ishizaki, Y.
Extracellular Vesicles from Vascular Endothelial Cells Promote Survival, Proliferation and Motility of Oligodendrocyte Precursor Cells. ,
PLoS ONE
 11 , e0159158 , 2016
Cells
Oligodendrocyte precursor cells
Enzymes
Papain : 16.5 u/ml
Medium
HBSS
Brain
Rat
Rat, SD, E18
Folts, C. , Scott-Hewitt, N. , Proschel, C. , Mayer-Proschel, M. and Noble, M.
Lysosomal Re-acidification Prevents Lysosphingolipid-Induced Lysosomal Impairment and Cellular Toxicity. ,
PLoS Biol
 14 , e1002583 , 2016
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
HBSS
Brain
Rat
Rat, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Rat
Rat, SD, neonatal
Kaiser, O. , Aliuos, P. , Wissel, K. , Lenarz, T. , Werner, D. , Reuter, G. , Kral, A. and Warnecke, A.
Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi After Digestion with Papain. ,
PLoS ONE
 8 , e80490 , 2013
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Neuro medium
Brain
Rat
Rat, Fisher, 7-21 month
Chen, N. , Newcomb, J. , Garbuzova-Davis, S. , Davis Sanberg, C. , Sanberg, P. and Willing, A.
Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro. ,
Aging Dis
 1 , 173 , 2010
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
Hibernate A
Brain
Rat
Rat, SD, 19-21 day
Cao, R. , Li, A. , Cho, H. , Lee, B. and Obrietan, K.
Mammalian Target of Rapamycin Signaling Modulates Photic Entrainment of the Suprachiasmatic Circadian Clock. ,
J Neurosci
 30 , 6302 , 2010
Cells
Suprachiasmatic nucleus neurons
Enzymes
Papain : 100 u/ml
Medium
MEM
Brain
Rat
Rat, SD, 7 day
Tanaka, S. , Shaikh, I. , Chiocca, E. and Saeki, Y.
The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development. ,
PLoS ONE
 4 , e5922 , 2009
Cells
Cerebellar granule neurons
Enzymes
PDS kit : per instructions
Medium
PBS
Brain
Rat
Rat, neonatal
Shigetomi, E. and Khakh, B.
Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes. ,
J Vis Exp
 26 , 1142 , 2009
Cells
Astrocytes
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Rat
Rat, SD
Akanda, N. , Tofighi, R. , Brask, J. , Tamm, C. , Elinder, F. and Ceccatelli, S.
Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells. ,
Cell Cycle
 7 , 3225 , 2008
Cells
Hippocampal
Enzymes
Trypsin : 0.1%
Medium
Neurobasal
Brain
Rat
Rat, neonatal
Richler Esther, Chaumont Severine, Shigetomi Eiji, Sagasti Alvaro, Khakh Baljit S.
Tracking transmitter-gated P2X cation channel activation in vitro and in vivo ,
Nat Methods
 5 , 87-93 , 2008
Cells
Hippocampal neurons
Enzymes
Papain : 10 u/ml
Medium
EBSS
Brain
Rat
Rat, neonatal
Peptan, I. , Hong, L. and Evans, C.
Multiple Differentiation Potentials of Neonatal Dura Mater-Derived Cells. ,
Neurosurgery
 60 , 346 , 2007
Cells
Dura mater
Enzymes
Medium
DMEM
Brain
Rat
Rat, E18
Jekabsons MB, Nicholls DG.
Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation ,
Cell Death Differ
 13 , 1595-610 , 2006
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Rat
Rat, Wistar, 1-3 day
Velasco Myrian, Garci­a Esperanza, Onetti Carlos G.
Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons ,
Cell Mol Neurobiol
 26 , 307-19 , 2006
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.05%
Medium
DMEM
Brain
Rat
Rat, E19
Khakh Baljit S, Fisher James A, Nashmi Raad, Bowser David N, Lester Henry A.
An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy ,
J Neurosci
 25 , 6911-20 , 2005
Cells
Hippocampal neuron
Enzymes
Papain : 10 u/ml
Medium
MEM
Brain
Rat
Rat, Wistar, 14 day
Mizoguchi Y, Kanematsu T, Hirata M, Nabekura J.
A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex ,
J Biol Chem
 278 , 44097 , 2003
Cells
Visual cortical
Enzymes
PDS kit : per instructions
Medium
EBSS
Brain
Rat
Rat, Wistar, male
Floris S, Van den Born J, van der Pol SM, Dijkstra CD, De Vries HE.
Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium ,
J Neuropathol Exp Neurol
 62 , 780 , 2003
Cells
Cerebral endothelial
Enzymes
Medium
MEM
Brain
Rat
Rat, SD, E18
Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I. and Barker, J
GABA(B) receptors mediate motility signals for migrating embryonic cortical cells ,
Cereb Cortex
 11 , 744-53 , 2001
Cells
Cortical cells
Enzymes
Papain : 20 u/ml
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.