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Tissue References Search Tool

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Adipose/Fat
Porcine
Porcine, crossbred, 1-3 day
Suryawan, A. , Swanson, L. and Hu, C.
Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture ,
J Anim Sci
 75 , 105 , 1997
Cells
Adipose, Stromal-vascular
Enzymes
Medium
DMEM/F12
Adipose/Fat
Rat
Rat, 9 week
Ohta, Y. , Hamaguchi, A. , Ootaki, M. , Watanabe, M. , Takeba, Y. , Iiri, T. , Matsumoto, N. and Takenaga, M.
Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury. ,
Cytotherapy
 19 , 839-848 , 2017
Cells
Adipose stromal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Adipose/Fat
Rat
Rat, SD, 2-3 month
Dayer, D. , Tabar, M. , Moghimipour, E. , Tabandeh, M. , Ghadiri, A. , Bakhshi, E. , Orazizadeh, M. and Ghafari, M.
Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells ,
Cytotherapy
 19 , 937-946 , 2017
Cells
Mesenchymal stromal
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat
Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G. and Karaoz, E
Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model. ,
Cytotherapy
 17 , 543-59 , 2015
Cells
Mesenchymal stem
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat
Ogasawara, J. , Izawa, T. , Sakurai, T. , Shirato, K. , Ishibashi, Y. , Ohira, Y. , Ishida, H. , Ohno, H. and Kizaki, T.
Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes. ,
Biochem Biophys Res Commun
 464 , 348-53 , 2015
Cells
Adipocytes
Enzymes
Medium
Krebs-Ringer
Adipose/Fat
Rat
Rat, SD, 5 month
Veronesi, F, Torricelli, P, Della, B, Pagani, S. and Fini, M
In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 215-23 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat, adult
Leblanc, A. , Nguyen, Q. , Touroo, J. , Aird, A. , Chang, R. , Ng, C. , Hoying, J. and Williams, S.
Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct. ,
Stem Cells Transl Med
 2 , 896-905 , 2013
Cells
Stromal vascular
Enzymes
Medium
PBS
Adipose/Fat
Rat
Rat, male, 300g
Thompson, A. , Nunez, M. , Davidson, R. , Horm, T. , Schnittker, K. , Hart, M. , Suarez, A. and Tsao, T.
Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue. ,
J Lipid Res
 53 , 2797 , 2012
Cells
Adipocytes
Enzymes
Medium
RPMI 1640
Adipose/Fat
Rat
Rat, Lewis, male
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Adipose/Fat
Rat
Rat
Wei, X. , Du, Z. , Zhao, L. , Feng, D. , Wei, G. , He, Y. , Tan, J. , Lee, W. , Hampel, H. , Dodel, R. , Johnstone, B. , March, K. , Farlow, M. and Du, Y.
IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats. ,
Stem Cells
 27 , 478 , 2009
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat
Tomiyama, K. , Murase, N. , Stolz, D. , Toyokawa, H. , O'Donnell, D. , Smith, D. , Dudas, J. , Rubin, J. and Marra, K.
Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue. ,
Stem Cells
 26 , 330 , 2008
Cells
Adipose tissue-derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat, Wistar, 4 week
Aoki, S. , Toda, S. , Sakemi, T. and Sugihara, H.
Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro ,
Cell Struct Funct
 28 , 55 , 2003
Cells
Adipocytes
Enzymes
Collagenase : 0.2%
Medium
Ham's F12
Adipose/Fat
Rat
Rat, SD
Mora, S. , Yang, C. , Ryder, J. , Boeglin, D. and Pessin, J
The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency ,
Endocrinology
 142 , 1999 , 2001
Cells
Adipocytes
Enzymes
Medium
KRHB
Adipose/Fat
Rat
Rat, SD, male, 4-7 weeks
Omatsu-Kanbe, M. and Matsuura, H.
Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes ,
J Physiol
 521 (3) , 601 , 1999
Cells
Brown adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat
Serrero, G.
Primary Culture in Defined Medium of Adipocyte Precursors , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 11B:6.1 , 1995
Cells
Adipocytes
Enzymes
Medium
DMEM /F-12
Adipose/Fat
Rat
Rat, SD, male, 130-160 g
Charron, M.J. and Kahn, B.B.
Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo ,
J Biol Chem
 265 , 7994 , 1990
Cells
Adipose
Epididymal fat pads
Enzymes
Collagenase : 0.3%
Medium
Kreb's-Ringer bicarbonate buffer modified
Adipose/Fat
Rat
Rat, 3 day
Gaben-Cogneville, A. , Poussin, B. , Chamblier, M. , Forgue-Fafitte, M. and Rosselin, G.
Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture ,
Biochim Biophys Acta
 968 , 231 , 1988
Cells
Preadipocytes
Enzymes
Medium
Parker Medium 199
Adipose/Fat
Rat
Rat, SD, various weights and ages
Woodward, Julie A. and Saggerson, E.
Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline ,
Biochem J
 238 , 395 , 1986
Cells
Brown adipocytes
Interscapular & cervical depots
Enzymes
Medium
Krebs Ringer bicarbonate buffer
Adipose/Fat
Rat
Rat, CD, male, 150-200 g
Pessin, J.E. , Gitomer, W. , Oka, Y. , Oppenheimer, C.L. and Czech, M.P.
ß-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes ,
J Biol Chem
 258 , 7386 , 1983
Cells
Adipocytes
Epididymal fat pads
Enzymes
Collagenase : 0.1%
Medium
Krebs Ringer bicarbonate buffer
Adipose/Fat
Rat
Rat, Wistar, albino, male, 100-140 g
Green, A. and Newsholme, E.
Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites ,
Biochem J
 180 , 365 , 1979
Cells
Adipocytes
Epididymal-fat pads
Enzymes
Collagenase : 0.3%
Medium
Kreb's Ringer
Adipose/Fat
Rat
Rat, Fischer, 344, male, 9 - 13 week
Stiles, J.W. , Francendese, A.A. and Masoro, E.J.
Influence of Age on Size and Number of Fat Cells in the Epididymal Depot ,
Am J Physiol
 229 , 1561 , 1975
Cells
White fat
Enzymes
Collagenase : 0.3%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Rat
Rat, Wistar, male, 5 week-16 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Rat
Rat (CFE), albino, female
Fain, J. , Reed, N. and Saperstein, R.
Isolation and Metabolism of Brown Fat Cells ,
J Biol Chem
 242 , 1887 , 1967
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Rat
Rat, SD, male, 160-210 g
Rodbell, M.
Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis ,
J Biol Chem
 239 (2) , 375 , 1964
Cells
Fat
Enzymes
Collagenase : 0.3%
Medium
Albumin-bicarbonate buffer
Adipose/Fat
Squirrel
Squirrel
McFarlane, S. , Mathers, K. and Staples, J.
Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator. ,
Am J Physiol Regul Integr Comp Physiol
 312 , R434-R442 , 2017
Cells
Brown adipocytes
Enzymes
Medium
Krebs-Ringer phosphate
Reproductive
Bovine
Bovine
Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R.
Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis ,
Endocrinology
 142 , 5254-60 , 2001
Cells
Corpus leuteal cells
Enzymes
Collagenase Type 4 : 420 u/ml
Medium
M-199
Reproductive
Bovine
Bovine, female
Tsang PC, Poff JP, Boulton EP, Condon WA.
Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro ,
Biol Reprod
 53 , 1160-8 , 1995
Cells
Leuteal
Enzymes
Medium
Ham's F-12
Reproductive
Bovine
Calf, mid to late gestational
Coplen, D. , Howard, P. , Duckett, J. , Snyder, H. and Macarak, E.
Characterization of a Fibroblast Cell From the Urinary Bladder Wall ,
In Vitro Cell Dev Biol
 30A , 604 , 1994
Cells
Fibroblasts
Enzymes
Collagenase : 0.1%
Medium
Medium 199
Reproductive
Bovine
Bovine
Munson, L. , Chandler, S. and Schlafer, D.
Long-Term Culture of Bovine Trophoblastic Cells ,
J Tiss Cul Meth
 11 (3) , 123 , 1988
Cells
Epithelial
Endometrial
Enzymes
Medium
DMEM/EBSS
Reproductive
Bovine
Bovine
(also porcine, human)
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Enzymatic Dissociation of Ovarian and Uterine Tissues ,
Endocr Res
 10 , 151 , 1984
Cells
Interna & corpus luteum
Endometrium
Ovarian
Uterine
Enzymes
Pronase : 0.1%
Medium
Moscona's BSS
Reproductive
Canine
Canine
Park, S. , Seo, M. , Kim, H. and Kang, K.
Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells. ,
PLoS ONE
 7 , e44693 , 2012
Cells
Amniotic membrane MSC
Enzymes
Trypsin : 0.25%
Medium
LG-DMEM
Reproductive
Chicken

Chicken, male, 1 day

Huang, H. , Wang, M. , Hou, L. , Lin, X. , Pan, S. , Zheng, P. and Zhao, Q.
A Potential Mechanism Associated with Lead-induced Spermatogonia and Leydig Cell Toxicity and Mitigative Effect of Selenium in Chicken. , Ecotoxicol Environ Saf Vol. 209 , 111671 , 2021
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Trypsin : 0.1%
Medium
DMEM
Reproductive
Chicken
Chicken, Gallus Domesticus,20-30 week
Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong.
An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles ,
J Reprod Dev
 52 , 569-76 , 2006
Cells
Primary follicles
Enzymes
Trypsin : 0.15%
Medium
Dulbecco's phosphate buffered saline
Reproductive
Fish
Sebastes Schlegel
Ryu, J. , Kim, H. , Bae, S. , Jung, C. and Gong, S.
Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of the Rockfish, Sebastes schlegeli ,
Fish Aquatic Sci
 19 , 2016
Cells
Ovarian
Enzymes
Collagenase Type 1 : 500 u/ml
Medium
L-15
Reproductive
Frog

Frog, X. laevis

Lavering, E. , Petros, I. and Weeks, D.
Component Analysis of Nucleolar Protein Compartments using Xenopus laevis Oocytes. , Dev Growth Differ Vol. 64 , 306-317 , 2022
Cells
Xenopus oocytes
Enzymes
Medium
Ovary Ringers Solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.