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Tissue References Search Tool

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Cartilage
Porcine
Porcine, 1 year
Chowdhury, T. , Schulz, R. , Rai, S. , Thuemmler, C. , Wuestneck, N. , Bader, A. and Homandberg, G
Biomechanical Modulation of Collagen Fragment-Induced Anabolic and Catabolic Activities in Chondrocyte/Agarose Constructs. ,
Arthritis Res Ther
 12 , R82 , 2010
Cells
Chondrocytes
Enzymes
Medium
DEMEM
Cartilage
Porcine
Porcine, 2-4 month
Graff RD, Lazarowski ER, Banes AJ, Lee GM.
ATP release by mechanically loaded porcine chondrons in pellet culture ,
Arthritis Rheum
 43 , 1571-9 , 2000
Cells
Chondrons
Enzymes
Medium
PBS
Cartilage
Rabbit

Rabbit, New Zealand, 2.7 kg

Khalilifar, M. , Eslaminejad, M. , Ghasemzadeh, M. , Hosseini, S. and Baharvand, H.
In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair. , Cell J Vol. 21 , 150-160 , 2019
Cells
Ear mesenchymal stem
Enzymes
Medium
DMEM
Cartilage
Rabbit
Rabbit, New Zealand, 1.2-1.4 kg
Ju, X. , Deng, M. , Ao, Y. , Yu, C. , Wang, J. , Yu, J. , Cui, G. and Hu, Y.
Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis. ,
Yakugaku Zasshi
 130 , 1053-60 , 2010
Cells
Chondrocytes
Enzymes
Pronase : 0.2%
Medium
DMEM
Cartilage
Rabbit
Rabbit, New Zealand, 1.8-2.3kg
Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.
Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion ,
Ann Rheum Dis
 56 , 108 , 1997
Cells
Chondrocytes
Enzymes
Medium
Gey's solution
Cartilage
Rabbit
Rabbit, New Zealand, white, 4-6 wk & 22-25 wk
Plaas, A. , Sandy, J. and Kimura, J.
Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes ,
J Biol Chem
 263 , 7560 , 1988
Cells
Chondrocytes
Enzymes
Protease XIV : 5 mg/g of tissue
Medium
Ham's F-12
Cartilage
Rabbit
Rabbit, white, male, 8 weeks
Benya, P.D. , Padilla, S.R. and Nimni, M.E.
The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II ,
Biochemistry
 16 , 865 , 1977
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Gey's BSS
Cartilage
Rabbit
Rabbit, New Zealand white or Dutch, 1 week
(also human, newborn)
Schindler, F.H. , Ose, M.A. and Solursh, M.
The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture ,
In Vitro
 12 , 44 , 1976
Cells
Chondrocytes
Enzymes
Trypsin : 0.1%
Medium
Saline G, CMF
Cartilage
Rabbit
Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg
Green, J.R. and William, T.
Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting ,
Clin Orthop Relat Res
 , 237 , 1976
Cells
Articular chondrocytes
Hyaline
Enzymes
Trypsin : 0.2%
Medium
Gey's BSS
Cartilage
Rabbit
Rabbit, New Zealand white, male, 250-350 g
Bentley, G. and Greer , R.
Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits ,
Nature
 230 , 385 , 1971
Cells
Epiphyseal
Articular cartilage
Enzymes
Trypsin : 0.25%
Medium
Eagle's basal medium
Cartilage
Rat
Rat
Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.
Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
 12 , e0176107 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Cartilage
Rat
Rat, SD, young, 100-120 g
Shimomura, Y. , Yoneda, T. and Suzuki, F.
Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
 19 , 179 , 1975
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Ham's F-12 medium
Liver
Avian
Avian, various
Else, P. , Brand, M. , Turner, N. and Hulbert, A
Respiration Rate of Hepatocytes Varies With Body Mass in Birds ,
J Exp Biol
 207 , 2305 , 2004
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Avian
Ducklings, 7 day
Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.
Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
 75(16) , 7651 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.05%
Medium
DMEM/ F12
Liver
Bovine

Bovine, Holstein, 22-23 month

Testroet, E. , Choudhary, S. , Choudhary, R. , Beitz, D. and Du, M.
Tumor Necrosis Factor Alpha and Palmitate Simulate Bovine Fatty Liver Disease in vitro when Using Abattoir-Derived Primary Bovine Hepatocytes Isolated by a Novel Nonperfusion Method. , JDS Commun Vol. 3 , 456-461 , 2022
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
DMEM
Liver
Canine
Dog, 13 years old
(also rat, guinea-pig, rabbit, human)
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Canine
Dog, adult
Vickrey, H. , Ramon, J. and McCann, D.
Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions ,
In Vitro
 15 , 120 , 1979
Cells
Hepatocytes
Enzymes
Trypsin : 0.1%
Medium
HBSS, CMF
Liver
Chicken
Chicken, adult
Fraslin, J. , Touquette, L. , Douaire, M. , Menezo, Y. , Guillemot, J. and Mallard, J.
Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture ,
In Vitro Cell Dev Biol
 28 , 615 , 1992
Cells
Hepatocytes
Enzymes
Collagenase : 0.02%
Medium
HEPES, CF
Liver
Chicken
Chicken
(also rat, SD, 150-250 g)
Weigel, P. , Schnaar, R. , Kuhlenschmidt, M. , Schmell, E. , Lee, R. , Lee, Y. and Roseman, S.
Adhesion of Hepatocytes to Immobilized Sugars ,
J Biol Chem
 254 (21) , 10830 , 1979
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 6000 units
Medium
HEPES
Liver
Chicken
Chicken, Leghorn, white, 10-15 day
Tarlow, D. , Watkins, P. , Reed, R. , Miller, R. , Zwergel, E. and Lane, M.
Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture ,
J Cell Biol
 73 , 332 , 1974
Cells
Hepatocytes
Enzymes
Deoxyribonuclease I : 0.00125%
Medium
PBS
Liver
Chicken
Chick embryos, 5 day
Steinberg, M.
"ECM": Its Nature, Origin, And Function in Cell Aggregation ,
Exp Cell Res
 30 , 257 , 1963
Cells
Heart
Liver
Enzymes
Trypsin : 3.0%
Medium
Tyrode's solution, CMF
Liver
Chicken
Chick embryonic
Rinaldini, L.
An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells ,
Exp Cell Res
 16 , 477 , 1959
Cells
Various tissues (heart, liver, skeletal, cardiac)
Enzymes
Trypsin : various grades
Medium
CMF Tyrode's solution
Liver
Equine
Equine, 4-13 yr
Bakala A. , Karlik W. and Wiechetek M.
Preparation of Equine Isolated Hepatocytes ,
Toxicol In Vitro
 17(5-6) , 615 , 2003
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Fish
Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g
Blair, J.B. , Miller, M.R. , Pack, D. , Barnes, R. , Teh, S.J. and Hinton, D.E.
Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions ,
In Vitro Cell Dev Biol
 26 , 237 , 1990
Cells
Hepatocytes
Enzymes
Medium
Kreb's-Ringer bicarbonate buffer, CF
Liver
Fish
Trout, male, 100 - 200 g
Lipsky, M. , Sheridan, T. , Bennett, R. and May, E.
Comparison of Trout Hepatocyte Culture on Different Substrates ,
In Vitro Cell Dev Biol
 22 , 360 , 1986
Cells
Hepatocytes
Enzymes
Medium
HBSS with 0.05M HEPES
Liver
Fish
Rainbow trout, (Salmo gairdneri), male, 150-200 g
Klaunig, J. , Ruch, R. and Goldblah, P.
Trout Hepatocyte Culture: Isolation and Primary Culture ,
In Vitro Cell Dev Biol
 21 , 221 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
HBSS/CMF
Liver
Fish
Trout 100g - 5kg
Bailey, G. , Taylor, M. and Selivonchick, D.
Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner ,
Carcinogenesis
 3 , 511 , 1982
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.08%
Medium
See reference
Liver
Fish
Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g
Klaunig, J.
Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies ,
Natl Cancer Inst Monogr
 65 , 163 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 100u/ml
Medium
HBSS
Liver
Guinea-Pig
Guinea-pig, Hartley, male, 250-300 g
Arinze, I. and Kawai, Y.
Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis ,
Arch Biochem Biophys
 225 , 196 , 1983
Cells
Hepatocytes
Medium
Kreb's Ringer bicarbonate buffer
Liver
Guinea-Pig
Guinea-pig, young
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Human

Human

Kucykowicz, S. , Amin, O. , Burton, A. , Swadling, L. , Schmidt, N. , Zakeri, N. , Davies, J. , Aidoo-Micah, G. , Stegmann, K. , Easom, N. , Jeffery-Smith, A. , Maini, M. and Pallett, L.
Isolation of Human Intrahepatic Leukocytes for Phenotypic and Functional Characterization by Flow Cytometry. ,
Star Protocols
 3 , 101356 , 2022
Cells
Intrahepatic leukocytes
Enzymes
Medium
RPMI-1640
Liver
Human
Human
Reuschel, E, Jilg, W. , Seelbach-Goebel, B. and Demi, L.
Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes ,
PLoS ONE
 , 2019
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Human
Human
Pfeiffer, E. , Kegel, V. , Zeilinger, K. , Hengstler, J. , Nussler, A. , Seehofer, D. and Damm, G.
Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells. ,
Exp Biol Med (Maywood)
 240 , 645-56 , 2015
Cells
Hepatocytes and non-parenchymal cells
Enzymes
Medium
HBSS
Liver
Human
Human
Werner, M. , Driftmann, S. , Kleinehr, K. , Kaiser, G. , Mathe, Z. , Treckmann, J. , Paul, A. , Skibbe, K. , Timm, J. , Canbay, A. , Gerken, G. , Schlaak, J. and Broering, R.
All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. ,
PLoS ONE
 10 , e0138655 , 2015
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Human
Human
Lee, S. , Schelcher, C. , Demmel, M. , Hauner, M. and Thasler, W.
Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. ,
J Vis Exp
 , 2013
Cells
Hepatocytes
Enzymes
Collagenase : 0.1-0.15%
Medium
HEPES
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.