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Tissue References Search Tool

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Liver
Mouse
Mouse, 10-12 week
Beldi, G. , Wu, Y. , Sun, X. , Imai, M. , Enjyoji, K. , Csizmadia, E. , Candinas, D. , Erb, L. and Robson, S.
Regulated Catalysis of Extracellular Nucleotides by Vascular CD39/ENTPD1 is Required for Liver Regeneration. ,
Gastroenterol
 135 , 1751 , 2008
Cells
Liver sinusoidal endothelial cells
Enzymes
Medium
HEPES
Liver
Mouse
Mouse, male
Oliva, J. , Bardag-Gorce, F. , French, B. , Li, J. , McPhaul, L. , Amidi, F. , Dedes, J. , Habibi, A. , Nguyen, S. and French, S.
Fat10 is an Epigenetic Marker for Liver Preneoplasia in a Drug-Primed Mouse Model of Tumorigenesis. ,
Exp Mol Pathol
 84 , 102 , 2008
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Elastase : 0.1 u/ml
Medium
Williams E
Liver
Mouse
Mouse
Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping.
Isolation and characterization of bipotent liver progenitor cells from adult mouse ,
Stem Cells
 24 , 322-32 , 2006
Cells
Liver epithelial progenitor cells
Enzymes
Medium
DMEM
Liver
Mouse
Mouse
Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev.
Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice ,
Hepatol
 42 , 140-8 , 2005
Cells
Liver sinusodial endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Liver
Mouse
Mouse
Jiang Guoqiang, Li Zhihua, Liu Franklin, Ellsworth Kenneth, Dallas-Yang Qing, Wu Margaret, Ronan John, Esau Christine, Murphy Cain, Szalkowski Deborah, Bergeron Raynald, Doebber Thomas, Zhang Bei.
Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1 ,
J Clin Invest
 115 , 1030-8 , 2005
Cells
Hepatocytes
Enzymes
Medium
Leffert's buffer
Liver
Mouse
Mouse, 8-10 week
Kotton, D. , Fabian, A. and Mulligan, R.
A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity. ,
Blood
 106 , 1574 , 2005
Cells
Liver derived stem cells
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, male, 8 week
Hatano E, Brenner DA.
Akt protects mouse hepatocytes from TNF-alpha- and Fas-mediated apoptosis through NK-kappa B activation. ,
Am J Physiol/Gastro
 282 , G1357 , 2002
Cells
Hepatocytes
Enzymes
Medium
Waymouth's medium
Liver
Mouse
Mouse
Sazani P. , Gemignani F. , Kang S. , Maier M. , Manoharan M. , Persmark M. , Bortner D. and Kole R.
Systemically Delivered Antisense Oligomers Up-regulate Gene Expression in Mouse Tissues ,
Nat Biotechnol
 20 , 1228 , 2002
Cells
Hepatocytes
Enzymes
Medium
DMEM/ F-12
Liver
Mouse
Mouse, male, 4-10 week
Lingohr Melissa K, Bull Richard J, Kato-Weinstein Junko, Thrall Brian D.
Dichloroacetate stimulates glycogen accumulation in primary hepatocytes through an insulin-independent mechanism ,
Toxicol Sci
 68 , 508-15 , 2002
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 100 u/ml
Medium
HBSS
Liver
Mouse
Mouse
Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O.
CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers ,
Drug Metab Dispos
 28 , 1321-6 , 2000
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Mouse
Mouse, 7 week
Angele MK, Knoferl MW, Schwacha MG, Ayala A, Cioffi WG, Bland KI. and Chaudry IH.
Sex steroids regulate pro- and anti-inflammatory cytokine release by macrophages after trauma-hemorrhage ,
Am J Physiol
 277 , C35 , 1999
Cells
Kupffer cells
Enzymes
Medium
HBSS
Liver
Mouse
Mouse
Ling W, Lougheed M, Suzuki H, Buchan A, Kodama T, Steinbrecher UP.
Oxidized or acetylated low density lipoproteins are rapidly cleared by the liver in mice with disruption of the scavenger receptor class A type I/II gene ,
J Clin Invest
 100 , 244-52 , 1997
Cells
Nonparenchymal liver
Enzymes
Medium
Hanks
Liver
Mouse
Mouse, C3H, 6-8 weeks
Lillehaug, J. , Mondal, S. and Heidelberger, C.
Establishment of Epithelial Cell Lines from Mouse Regenerating Liver ,
In Vitro
 15 , 910 , 1979
Cells
Epithelial
Enzymes
Collagenase : 0.10%
Medium
DMEM
Liver
Mouse
Mouse, 20-30 g
Crisp, D. and Pogson, C.
Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver ,
Biochem J
 126 , 1009 , 1972
Cells
Parenchymal and non-parenchymal
Enzymes
Hyaluronidase : 0.1%
Medium
Hank's w/ Insulin, CMF
Liver
Porcine

Porcine

Almalla, A. , Elomaa, L. , Bechtella, L. , Daneshgar, A. , Yavvari, P. and Mahfouz, Z.
Papain-Based Solubilization of Decellularized Extracellular Matrix for the Preparation of Bioactive, Thermosensitive Pregels. , Biomacromolecules Vol. 24 , 5620-5637 , 2023
Cells
Decellularized Extracellular Matrix
Enzymes
Papain : 0.05%
Medium
PBS
Liver
Porcine
Porcine, mini-pig, 13 kg
Meng, F. , Chen, Z. , Han, M. , Hu, X. , He, X. , Liu, Y. , He, W. , Huang, W. , Guo, H. and Zhou, P.
Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination. ,
World J Gastroenterol
 16 , 1660 , 2010
Cells
Hepatocytes
Enzymes
Deoxyribonuclease I : see reference
Medium
Williams E
Liver
Porcine
Porcine, Yorkshire, male, 21 day
Terner, M. , Gilmore, W.J. , Lou, Y. and Squires, E.J.
The Role of CYP2A and CYP2E1 in the Metabolism of 3-Methylindole in Primary Cultured Porcine Hepatocytes. ,
Drug Metab Dispos
 34 , 848 , 2006
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Porcine
Porcine, Chinese mini pig, 6-10 kg
Li, J, Li, L. , Chao, H. , Yang, Q. , Liu, X. , Sheng, J. , Yu, H. and Huang, J
Isolation and Short Term Cultivation of Swine Hepatocytes for Bioartificial Liver Support System. ,
Hepatobiliary Pancreat Dis Int
 4 , 249 , 2005
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 125 u/ml
Medium
Williams E
Liver
Porcine
Porcine, male, 2-3 week
Raman Priya, Donkin Shawn S, Spurlock Michael E.
Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures ,
Am J Physiol Regul Integr Comp Physiol
 286 , R206-16 , 2004
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
DMEM
Liver
Porcine
Porcine, one week
Wang Y. , Liu H. , Guo H. , Wen H. and Liu J.
Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich ,
World J Gastroenterol
 10 , 699 , 2004
Cells
Hepatocytes
Enzymes
Medium
RPMI 1640
Liver
Porcine
Porcine, adult
Zhou X, Liu L, Kano J, Mukaiyama T. and Tokiwa T.
Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System ,
Chin Med J
 114 , 946 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
MEM
Liver
Porcine
Porcine, male, 6-40 kg
Gerlach, J. , Brombacher, J. , Smith, M. and Neuhaus, P.
High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight ,
J Surg Res
 62 (1) , 85 , 1996
Cells
Hepatocytes
Enzymes
Collagenase : 0.8%
Medium
PBS
Liver
Rabbit
Rabbit, New Zealand white
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Rat

Rat, 8-14 week

Ge, J. , Zheng, Y. , Tsuchida, T. , Furuya, K. , Isoda, H. , Taniguchi, H. , Ohkohchi, N. and Oda, T.
Hepatic Stellate Cells Contribute to Liver Regeneration through Galectins in Hepatic Stem Cell Nliche. ,
Stem Cell Res Ther
 11 , 425 , 2020
Cells
Parenchymal and non-parenchymal
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Rat

Wistar, male, 200-250 g

Berardo, C. , Ferrigno, A. , Siciliano, V. , Richelmi, P. , Vairetti, M. and Pasqua, L.
Isolation of Rat Hepatocytes for Pharmacological Studies on Metabotropic Glutamate Receptor (mGluR) Subtype 5: A Comparison Between Collagenase I Versus Collagenase IV. ,
Eur J Histochem
 64 , 2020
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat, SD, 500-700g
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Rat
Rat, adult
Shen, L. , Hillebrand, A. , Wang, D. and Liu, M.
Isolation and Primary Culture of Rat Hepatic Cells. ,
J Vis Exp
 64 , e3917 , 2012
Cells
Hepatocytes
Enzymes
Medium
William's
Liver
Rat
Rat, adult
Wen, J. , Olsen, A. , Perepelyuk, M. and Wells, R.
Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion. ,
J Vis Exp
 64 , e3669 , 2012
Cells
Portal fibroblasts
Enzymes
Medium
DMEM/F-12
Liver
Rat
Rat
Budick-Harmelin, N. , Anavi, S. , Madar, Z. and Tirosh, O.
Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes. ,
Lipids Health Dis
 11 , 66 , 2012
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat, SD, 240-320g
Pillai, V. and Mehvar, R.
Inhibition of NADPH-Cytochrome P450 Reductase by Tannic Acid in Rat Liver Microsomes and Primary Hepatocytes: Methodological Artifacts and Application to Ischemia-Reperfusion Injury ,
J Pharm Sci
 100 , 3495 , 2011
Cells
Hepatocytes
Enzymes
HIS kit : per instructions
Medium
Waymouth's MB
Liver
Rat
Rat
Gopalakrishnan, S. and Harris, E.
In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. ,
J Vis Exp
 57 , e3138 , 2011
Cells
Hepatocytes and SEC's
Enzymes
Collagenase Type 1 : 0.1-0.2%
Medium
RPMI
Liver
Rat
Rat, SD, 250-300g
Parasrampuria, R. and Mehvar, R.
Dose-Dependent Inhibition of Transporter-Mediated Hepatic Uptake and Biliary Excretion of Methotrexate by Cyclosporine A in an Isolated Perfused Rat Liver Model. ,
J Pharm Sci
 99 , 5060 , 2010
Cells
Hepatocytes
Enzymes
HIS kit : per instructions
Medium
Krebs-Henseleit
Liver
Rat
Rat, SD
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Cells
Hepatic stellate cells
Enzymes
Pronase : 0.02%
Collagenase : see reference
Medium
Medium 199
Liver
Rat
Rat, Wistar, female
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Cells
Hepatic stellate cells
Enzymes
Collagenase Type 1 : 0.025-0.1%
Pronase : 0.025-0.13%
Medium
DMEM
Liver
Rat
Rat, SD
Chung, C. , Shugrue, C. , Nagar, A. , Doll, J. , Cornwell, M. , Gattu, A. , Kolodecik, T. , Pandol, S. and Gorelick, F.
Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator. ,
Gastroenterol
 136 , 331 , 2009
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Lewis, male
Bettinger, C. , Kulig, K. , Vacanti, J. , Langer, R. and Borenstein, J.
Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture. ,
Tissue Eng Part A
 15 , 1321 , 2009
Cells
Hepatocytes
Enzymes
Medium
DPBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.