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Tissue References Search Tool

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Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Human
Human
Mackay, L. , Dodd, S. , Dougall, I. , Tomlinson, W. , Lordan, J. , Fisher, A. and Corris, P.
Isolation and Characterisation of Human Pulmonary Microvascular Endothelial Cells from Patients with Severe Emphysema. ,
Respir Res
 14 , 23 , 2013
Cells
Pulmonary endothelial
Enzymes
Medium
RPMI
Lung
Human
Human
Comhair, S. , Xu, W. , Mavrakis, L. , Aldred, M. , Asosingh, K. and Erzurum, S.
Human Primary Lung Endothelial Cells in Culture. ,
Am J Respir Cell Mol Biol
 46 , 723-30 , 2012
Cells
Lung endothelial
Enzymes
Medium
PBS
Lung
Human
Human
Fujino, N. , Kubo, H. , Suzuki, T. , Ota, C. , Hegab, A. , He, M. , Suzuki, S. , Suzuki, T. , Yamada, M. , Kondo, T. , Kato, H. and Yamaya, M.
Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs. ,
Lab Invest
 91 , 363 , 2011
Cells
Lung
Enzymes
Medium
DMEM
Lung
Human
Human
Zhu, Y. , Skold, C. , Liu, X. , Wang, H. , Kohyama. , Wen, F. , Ertl, R. and Rennard, S.
Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture ,
Respir Res
 2 (5) , 295 , 2001
Cells
Lung
Fibroblasts
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Human
Human, 16-24 week therapeutic abortions
Liley, H. , Ertsey, R. , Gonzales, L. , Odom, M. , Hawgood, S. , Dobbs, L. and Ballard, P.
Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung ,
Biochim Biophys Acta
 961 , 86 , 1988
Cells
Alveolar type II
Fetal
Enzymes
Trypsin : 50 ug/ml
Medium
Ham's F-12, Eagle's MEM
Lung
Human
Human
Lechner, J. , Haugen, A. , McClendon, I. and Pettis, E.
Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium ,
In Vitro
 18 (7) , 633 , 1982
Cells
Epithelial
Enzymes
Trypsin : 0.02%
Medium
Medium 199
Lung
Human
Human, 12-16 wk old embryo
Kan, M. and Yamane, I.
In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium ,
J Cell Physiol
 111 , 155 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.01%
Medium
Eagle's MEM
Lung
Human
Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Lung
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Lung
Mouse

Mouse

Horn, K. , Fulte, S. , Yang, M. , Lorenz, B. and Clark, S.
Neutrophil Responsiveness to IL-10 Impairs Clearance of Streptococcus pneumoniae from the Lungs. ,
J Leukoc Biol
 , 2023
Cells
Lung
Enzymes
Medium
PBS
Lung
Mouse

Mouse

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Qiu, Y. , Xu, Y. , Wang, M. , Miao, M. , Zhou, H. , Xu, J. , Kong, J. , Zheng, D. , Li, R. , Zhang, R. , Guo, Y. , Li, X. , Cui, J. , Qin, C. and Zhou , X.
Flavivirus Induces and Antagonizes Antiviral RNA Interference in Both Mammals and Mosquitoes. ,
Sci. Adv.
 6 , 7989 , 2020
Cells
Fibroblasts
Enzymes
Medium
HBSS
Lung
Mouse
2-3 month
Wang, J. , Niu, N. , Xu, S. and Jin, Z.
A Simple Protocol for Isolating Mouse Lung Endothelial Cells. ,
Sci Rep
 9 , 1458 , 2019
Cells
Lung endothelial
Enzymes
Medium
DMEM
Lung
Mouse
Mouse, 6 week
Alves CarlosRoberto, Souza RaquelSantos de, Charret KarenDos Santos, Côrtes LuziaMonteiro de Castro, Sá-Silva MatheusPereira de, Barral-Veloso Laura, Oliveira LuizFilipe Gonçalves, da Silva FranklinSouza.
Understanding serine proteases implications on Leishmania spp lifecycle. ,
Exp Parasitol
 184 , 67-81 , 2018
Lung
Mouse
Mouse, 6 week
Lewis, K. , Hall, J. , Kiyotake, E. , Christensen, T. , Balasubramaniam, V. and Anseth, K.
Epithelial-Mesenchymal Crosstalk Influences Cellular Behavior in a 3D Alveolus-Fibroblast Model System. ,
Biomaterials
 155 , 124 , 2018
Cells
Alveolar epithelial
Enzymes
Medium
DMEM/F-12
Lung
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Lung
Mouse
Mouse, 8-12 week
Singer, B. , Mock, J. , D'Alessio, F. , Aggarwal, N. , Mandke, P. , Johnston, L. and Damarla, M.
Flow-Cytometric Method for Simultaneous Analysis of Mouse Lung Epithelial, Endothelial, and Hematopoietic Lineage Cells. ,
Am J Physiol Lung Cell Mol Physiol
 310 , L796-801 , 2016
Cells
Epithelial, Endothelial
Enzymes
Medium
PBS
Lung
Mouse
Mouse
Yu, Y. , O'Koren, E. , Holten, D. , Kan, M. , Kojin, D. , Nelson, E. , Que, L. and Gunn, M.
A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflammed Murine Non-Lymphoid Tissues ,
PLoS ONE
 10 , 1371 , 2016
Cells
Lung
Enzymes
Medium
PBS
Lung
Mouse
Mouse
Braza, F. , Dirou, S. , Forest, V. , Sauzeau, V. , Hassoun, D. , Chesne, J. , Cheminant-Muller, M. , Sagan, C. , Magnan, A. and Lemarchand, P.
Mesenchymal Stem Cells Induce Suppressive Macrophages Through Phagocytosis in a Mouse Model of Asthma. ,
Stem Cells
 34 , 1836-45 , 2016
Cells
Lung
Enzymes
Medium
PBS
Lung
Mouse
Mouse
Mohapatra, A. , Van Dyken, S. , Schneider, C. , Nussbaum, J. , Liang, H. and Locksley, R.
Group 2 Innate Lymphoid Cells Utilize the IRF4-IL-9 Module to Coordinate Epithelial Cell Maintenance of Lung Homeostasis. ,
Mucosal Immunol
 , 2015
Cells
Alveolar
Enzymes
Collagenase Type 1 : 300 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Lung
Mouse
Mouse, embryonic
Desai, T. , Brownfield, D. and Krasnow, M.
Alveolar Progenitor and Stem Cells in Lung Development, Renewal and Cancer. ,
Nature
 507 , 190-4 , 2014
Cells
Avelolar progenitor
Enzymes
Elastase : 3 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Kim, H. , Lee, H. , Chang, Y. , Pichavant, M. , Shore, S. , Fitzgerald, K. , Ikawura, Y. , Israel, E. , Bolger, K. , Faul, J. , DeKruyff, R. and Umetsu, D.
IL-17 Producing Innate Lymphoid Cells and the NLRP3 Inflammasone Facilitate Obescity-associated Airway Hyperractivity ,
Nat Med
 20 , 54 , 2014
Cells
Pulmonary
Enzymes
Medium
see reference
Lung
Mouse
Mouse
Misharin, A. , Morales-Nebreda, L. , Mutlu, G. , Budinger, G. and Perlman, H.
Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung. ,
Am J Respir Cell Mol Biol
 49 , 503-10 , 2013
Cells
Lung
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Guimond, D. , Cam, N. , Hirve, N. , Duan, W. , Lambris, J. , Croft, M. and Tsoukas, C.
Regulation of Immune Responsiveness In Vivo by Disrupting an Early T-Cell Signaling Event Using a Cell-Permeable Peptide. ,
PLoS ONE
 8 , e63645 , 2013
Cells
Lung
Enzymes
Medium
PBS
Lung
Mouse
Mouse
Vaughan, A. , Halbert, C. , Wootton, S. and Miller, A.
Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation. ,
Mol Cancer Res
 10 , 86 , 2012
Cells
Lung tumor
Enzymes
Neutral Protease : 50 u/ml
Collagenase : 400 u/ml
Medium
DMEM
Lung
Mouse
Mouse
Hardy, C. , LeMasurier, J. , Belz, G. , Scalzo-Inguanti, K. , Yao, J. , Xiang, S. , Kanellakis, P. , Bobik, A. , Strickland, D. , Rolland, J. , O'Hehir, R. and Plebanski, M.
Inert 50-nm Polystyrene Nanoparticles That Modify Pulmonary Dendritic Cell Function and Inhibit Allergic Airway Inflammation. ,
J Immunol
 188 , 1431 , 2012
Cells
Lung
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse, 8-12 week
Driscoll, B. , Kikuchi, A. , Lau, A. , Lee, J. , Reddy, R. , Jesudason, E. , Kim, C. and Warburton, D.
Isolation and Characterization of Distal Lung Progenitor Cells. ,
Methods Mol Biol
 879 , 109 , 2012
Cells
Lung progenitor
Enzymes
Medium
DMEM
Lung
Mouse
Mouse, 8-10 week
Chow, K. , Jun, D. , Helm, K. , Wagner, D. and Majka, S.
Isolation & Characterization of Hoechst(low) CD45(negative) Mouse Lung Mesenchymal Stem Cells. ,
J Vis Exp
 56 , e3159 , 2011
Cells
Lung mesenchymal stem
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Rock, J, Barkauskas, C, Cronc, M, Xue, Y, Harris, J, Liang, J, Noble, P. and Hogan, B.
Multiple Stromal Populations Contribute to Pulmonary Fibrosis Without Evidence for Epithelial to Mesenchymal Transition. ,
Proc Natl Acad Sci U S A
 108 , E1475 , 2011
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Rayamajhi, M. , Redente, E. , Condon, T. , Gonzalez-Juarrero, M. , Riches, D. and Lenz, L.
Non-Surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry. ,
J Vis Exp
 51 , 2702 , 2011
Cells
Lung draining
Enzymes
Collagenase Type 4 : 0.1-0.125%
Medium
HBSS
Lung
Mouse
Mouse
Lancelin, W. and Guerrero-Plata, A.
Isolation of Mouse Lung Dendritic Cells. ,
J Vis Exp
 57 , e3563 , 2011
Cells
Dendritic
Enzymes
Medium
HBSS
Lung
Mouse
Mouse, female, 6-12 week
Breslow, R. , Rao, J. , Xing, W. , Hong, D. , Barrett, N. and Katz, H.
Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells. ,
J Immunol
 184 , 1003 , 2010
Cells
Lung mononuclear
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Ferreira, C. , Antunes, F. , Leonard, V. , Welstead, G. , Richardson, C. and Cattaneo, R.
Measles Virus Infection of Alveolar Macrophages and Dendritic Cells Precedes Spread to Lymphatic Organs in Transgenic Mice Expressing Human Signaling Lymphocytic Activation Molecule (SLAM, CD150). ,
J Virol
 84 , 3033 , 2010
Cells
Lung
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse, 6-7 week
Jungblut, M. , Oeltze, K. , Zehnter, I. , Hasselmann, D. and Bosio, A.
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue Using the GentleMACS Dissociator. ,
J Vis Exp
 29 , 2009
Cells
Leukocytes and endothelial
Enzymes
Collagenase : 0.2%
Deoxyribonuclease I : 40-80 u/ml
Medium
PBS
Lung
Mouse
Mouse
Flano, E. , Jewell, N. , Durbin, R. and Durbin, J.
Methods Used to Study Respiratory Virus Infection. , Curr Protoc Cell Biol Vol. Chapter 26 , Unit 26.3 , 2009
Cells
Dendritic
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.