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Tissue References Search Tool

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Reproductive
Mouse
Mouse, male, 10-13 wk
Stalvcey, J. and Payne, A.
Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains ,
Endocrinology
 112 , 1696 , 1983
Cells
Leydig
Testis
Enzymes
Medium
Medium 199 w/ BSA
Reproductive
Mouse
Mouse (BALB/cCrgl), female, 50 - 60 days
Iguchi, T. , Uchima, F.A. , Ostrander, P. and Bern, H.
Growth of Normal Mouse Vaginal Epithelial Cells in and on Collagen Gels ,
Proc Natl Acad Sci U S A
 80 , 3743 , 1983
Cells
Epithelial, vagina
Enzymes
Medium
HBSS
Reproductive
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Reproductive
Ovine
Sheep
Salamonsen, L. , Sum O, W. , Doughton, B. and Findlay, J.
The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium ,
Endocrinology
 117 (5) , 2148 , 1985
Cells
Epithelial
Enzymes
Collagenase : 125 - 190 u/ml
Medium
DMEM
Reproductive
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Reproductive
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Reproductive
Porcine
Porcine, female
Ciereszko, R. , Petroff, B. , Ottobre, A. , Guan, Z. , Stokes, B. and Ottobre, J.
Assessment of the Mechanism by Which Prolactin Stimulates Progesterone Production by Early Corpora Lutea of Pigs ,
J Endocrinol
 159 , 201 , 1998
Cells
Corpus Leuteum
Enzymes
Collagenase Type 4 : 600 u/ml
Medium
Medium 199
Reproductive
Porcine
Porcine (also bovine, human)
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Enzymatic Dissociation of Ovarian and Uterine Tissues ,
Endocr Res
 10 , 151 , 1984
Cells
Interna & corpus luteum
Endometrium
Ovarian
Uterine
Enzymes
Pronase : 0.1%
Medium
Moscona's BSS
Reproductive
Porcine
Porcine, 3-4 week
Mather, J. , Saez, J. and Haour, F.
Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells ,
Endocrinology
 110 , 933 , 1982
Cells
Leydig
Testis
Enzymes
Trypsin : 0.0003%
Medium
Lebovitz L-15 Medium
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white, 4-6 month old,
Setrakian, S. , Oliveros-Saunders, B. and Nicosia, S.
Growth Stimulation of Ovarian and Extraovarian Mesothelial Cells by Corpus Luteum Extract ,
In Vitro Cell Dev Biol
 29A , 879 , 1993
Cells
Ovarian
Mesothelial
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white, adult, female (nonpregnant),
3-4 kg
Phillippe, M. , Saunders, T. and Bangalore, S.
Alpha-1,Alpha-2,and Beta Adrenergic Signal Transduction in Cultured Uterine Myocytes ,
In Vitro Cell Dev Biol
 26 , 369 , 1990
Cells
Myocytes, uterine
Enzymes
Deoxyribonuclease I : 200 µg/ml
Medium
HBSS-HEPES buffer
Reproductive
Rabbit
Rabbit, New Zealand, mature, female, 4-5 Kg
Piquette, G. and Timms, B.
Isolation and Characterization of Rabbit Ovarian Surface Epithelium, Granulosa Cells, and Peritoneal Mesothelium in Primary Culture ,
In Vitro Cell Dev Biol
 26 , 471 , 1990
Cells
Ovarian surface epithelial and peritoneal mesothelial
Enzymes
Collagenase Type 1 : 300 IU/ml (280 IU/mg)
Medium
HBSS, CMF
Reproductive
Rabbit
Rabbit, mixed breed
Mulholland, J. , Winterhager, E. and Beier, H.
Changes in Proteins Synthesized by Rabbit Endometrial Epithelial Cells Following Primary Culture ,
Cell Tissue Res
 252 , 123 , 1988
Cells
Endometrial epithelial
Enzymes
Medium
DMEM
Reproductive
Rabbit
Rabbit, New Zealand white, mature, 3-4 Kg
Boulet, A. and Fortier, M.
Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment ,
In Vitro Cell Dev Biol
 23 , 93 , 1987
Cells
Myometrial
Enzymes
Trypsin : 0.02%, 0.03%, 0.0375%
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Nicosia, S. , Johnson, J. and Streibel, E.
Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) ,
Int J Gynecol Pathol
 3 , 348 , 1984
Cells
Mesothelial and surface epithelial
Ovaries
Enzymes
Trypsin : 0.125%-0.5%
Medium
Medium 199
Reproductive
Rat
Rat
Jiang, H. , Zhang, Y. , Tian, K. , Wang, B. and Han, S.
Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells. ,
Sci Rep
 7 , 41837 , 2017
Cells
Placental mesenchymal
Enzymes
Medium
DMEM
Reproductive
Rat
Rat, SD, 350-450 g
Sharma RS, Pal PC, Rajalakshmi M.
Isolation and Culture of Leydig Cells from Adult Rats ,
Indian J Clinical Biochem
 21 , 27 , 2006
Cells
Leydig cells
Enzymes
Collagenase : .05-0.1%
Medium
Medium 199
Reproductive
Rat
Rat, SD, adult, male, 8 - 10 week
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Reproductive
Rat
Rat, SD, immature
Ando, M. , Kol, S. , Irahara, M. , Sirois, J. and Adashi, E.
Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process ,
Mol Cell Endocrinol
 157 , 21 , 1999
Cells
Ovary
Enzymes
Collagenase Type 1 : 144 u/ml
Medium
McCoy's 5a
Reproductive
Rat
Rat, Han-Wistar, adult, male, 250 g
Leckie, C. , Welberg, L. and Seckl, J.
11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells ,
J Endocrinol
 159 , 233 , 1998
Cells
Testes
Enzymes
Trypsin : 0.1%
Medium
Medium 199 w/ Hank's salts
Reproductive
Rat
Rat, SD, female, immature, 26 day
(also rat, pregnant and pseudopregnant)
Tellieria, C. , Ou, J. , Sugino, N. , Ferguson, S. and Gibori, G.
The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid ,
Endocrinology
 139 (8) , 3597 , 1998
Cells
Corpus luteum
Enzymes
Neutral Protease : 2.4 u/ml Deoxyribonuclease: 200u/ml
Medium
Serum-free medium
(see reference)
Reproductive
Rat
Rat, SD, 25 day
Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR.
Effects of insulin and insulin-like growth factors on proliferation of rat ovarian theca-interstitial cells ,
Biol Reprod
 56 , 891-7 , 1997
Cells
Ovarian theca-interstitial
Enzymes
Medium
Medium 199
Reproductive
Rat
Rat, SD, male, 90 days
Onoda, M. and Djakiew, D.
Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin ,
In Vitro Cell Dev Biol
 27 , 215 , 1991
Cells
Sertoli
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM
Reproductive
Rat
Rat, SD, male
Abayasekara, D. , Kurlak, L. , Band, A. , Sullivan, M. and Cooke, B.
Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis ,
In Vitro Cell Dev Biol
 27 , 253 , 1991
Cells
Leydig
Enzymes
Trypsin : 0.02%
Medium
DMEM
Reproductive
Rat
Rat, neonatal
Branham, W. , Lyn-Cook, B. , Andrews, A. , McDaniel, M. and Sheehan, D.
Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix ,
In Vitro Cell Dev Biol
 27 , 442 , 1991
Cells
Uterine
Enzymes
Trypsin : 1%
Medium
HBSS
Reproductive
Rat
Rat, Wistar, adult
Pampfer, S. , Vanderheyden, I. , Michiels, B. and DeHertogh, R.
Co-Culture of Two-Cell Rat Embryos on Cell Monolayers ,
In Vitro Cell Dev Biol
 26 , 944 , 1990
Cells
Uterine
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, 10 day
Hadley MA, Weeks BS, Kleinman HK, Dym M.
Laminin promotes formation of cord-like structures by Sertoli cells in vitro ,
Dev Biol
 140 , 318-27 , 1990
Cells
Sertoli cells
Enzymes
Medium
PBS
Reproductive
Rat
Rat, SD, male, 120-160 g
Ng, T. and Liu, W.
Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis ,
In Vitro Cell Dev Biol
 26 , 24 , 1990
Cells
Leydig
Adrenal
Enzymes
Collagenase Type 2 : 0.03% (adrenal)
Medium
Krebs Ringer bicarbonate buffer
Reproductive
Rat
Rat, immature
Rajan, V. and Menon, K.
Differential Uptake and Metabolism of Free and Esterified Cholesterol from High-density Lipoproteins in the Ovary ,
Biochim Biophys Acta
 959 , 206 , 1988
Cells
Luteal, ovaries
Enzymes
Collagenase : 0.3%
Medium
McCoy's
Reproductive
Rat
Rat, SD, female
Glasser, S. , Julian, J. , Decker, G. , Tang, J. and Carson, D.
Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells ,
J Cell Biol
 107 (6) , 2409 , 1988
Cells
Luminal epithelial
Enzymes
Trypsin : 0.5%
Medium
HBSS
Reproductive
Rat
Rat, male, 20 day
Skinner, M. , Fetterolf, P. and Anthony, C.
Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function ,
J Biol Chem
 263 , 2884 , 1988
Cells
Sertoli
Enzymes
Trypsin : 0.15%
Medium
(see reference)
Reproductive
Rat
Rat, Wistar, virgin, female, 250 g
Conti, C. and Tasat, D.
Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone ,
J Steroid Biochem
 24 (3) , 747 , 1986
Cells
Vaginal epithelial
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, female, 21 day
Rajan, V. and Menon, K.
Involvement of Microtubules in Lipoprotein Degradation and Utilization for Steroidogenesis in Cultured Rat Luteal Cells ,
Endocrinology
 117 , 2408 , 1985
Cells
Luteal, ovaries
Enzymes
Medium
McCoy's
Reproductive
Rat
Rat, SD, male, 10 day
Hadley, M. , Byers, S. , Suarez-Quian, C. , Kleinman, H. and Dym, M.
Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro ,
J Cell Biol
 101 , 1511 , 1985
Cells
Sertoli
Enzymes
Trypsin : 0.025%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.