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Mammary
Mouse
Mouse, BALB/cfC3H/Crgl, virgin, female
DeOme, K. , Miyamoto, M. , Osborn, R. , Guzman, R. and Lum, K.
Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice ,
Cancer Res
 38 , 2103 , 1978
Cells
Nodule-transformed
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Mouse
Mouse (BALB/cCrgl), virgin, female, 4-8 month
White, M. , Hu, A. , Hamamoto, S. and Nandi
In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage ,
In Vitro
 14 , 271 , 1978
Cells
Epithelial
Mammary gland
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, BALB/cCrgl, female
(also guinea-pig)
Asch, B. and Medina, D.
Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells ,
J Natl Cancer Inst
 61 (6) , 1423 , 1978
Cells
Swiss 3T3
Enzymes
Medium
PBS CMF
Mammary
Mouse
Mouse, (C3H/Crgl or BALB/cCrgl), 8-10 day pregnant
Emerman, J. , Enami, J. , Pitelka, D. and Nandi, S.
Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes ,
Proc Natl Acad Sci U S A
 74 (10) , 4466 , 1977
Cells
Epithelial
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, lactating
Kerkof, P. and Abraham, S.
Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice ,
Methods Enzymol
 69 , 693 , 1976
Cells
Parenchymal
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Mammary
Mouse
Mouse, female, early pregnancy <10 days
Moore, D. and Lasfargues, E.
Method for the Continuous Cultivation of Mammary Epithelium ,
In Vitro
 7 , 21 , 1971
Cells
Epithelial
Enzymes
Collagenase : 0.1%
Medium
Eagle's MEM
Mammary
Mouse
Mouse, CBA, virgin, 9/10 wk
Prop, F. and Wiepjes, G.
Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse ,
Exp Cell Res
 61 , 451 , 1970
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
BSS, CMF
Mammary
Mouse
Mouse, lactating, 14-18 day
Pitelka, D. , Kerkof, P. , Gagne, H. , Smith, S. and Abraham, S.
Characteristics of Cells Dissociated from Mouse Mammary Glands. I. Method of Separation and Morphology of Parenchymal Cells from Lactating Glands ,
Exp Cell Res
 57 , 43 , 1969
Cells
Parenchymal
Enzymes
Collagenase : 0.33%
Medium
Kreb's buffer
Mammary
Mouse
Mouse, lactating, 14 day
Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I.
Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland ,
Cancer Res
 26 , 1534 , 1966
Cells
Mammary
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, pregnant, 14-17 day
Daniel, C. and DeOme, K.
Growth of Mouse Mammary Glands In Vivo After Monolayer Culture ,
Science
 149 , 634 , 1965
Cells
Mammary
Enzymes
Collagenase : 0.05% - 0.1%
Medium
HBSS
Mammary
Mouse
Mouse, adult, 1-10 days pregnant
Lasfargues, E.
Cultivation and Behavior In Vitro of the Normal Mammary Epithelium of the Adult Mouse. II. Observations on the Secretory Activity ,
Exp Cell Res
 13 , 553 , 1957
Cells
Epithelial
Mammary
Enzymes
Collagenase : 0.02%
Medium
Simm's
Mammary
Rat
Rat
Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.
The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
 115 , 412 , 2010
Cells
Mammary epithelial
Enzymes
Medium
HBSS
Mammary
Rat
Rat, female, 50 day
Maffini M. , Soto A. , Calabro J. , Ucci A. and Sonnenschein C.
The Stroma as a Crucial Target in Rat Mammary Gland Carcinogenesis ,
J Cell Sci
 117 , 1495 , 2004
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat, female
Djuric Z. , Lewis S. , Mayhugh M. , Naegeli L. , Tang N. and Hart R.
Effect of Varying Caloric Restriction Levels on Female Rat Growth and 5-hydroxymethyl-2'-deoxyuridine in DNA ,
Toxicol Sci
 66 , 125 , 2002
Cells
Mammary gland epithelial
Enzymes
Medium
see reference
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12
Mammary
Rat
Rat, SD, 50 day
Varela L. , Darcy K. and Ip M.
The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells ,
Endocrinology
 138 , 3891 , 1997
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat
(also human)
Soriano, J. , Pepper, M. , Nakamura, T. , Orci, L. and Montesano, R.
Hepatocyte Growth Factor Stimulates Extensive Development of Branching Duct-like Structures by Cloned Mammary Gland Epithelial Cells ,
J Cell Sci
 108 , 413-430 , 1995
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Rat
Rat, SD, female, 60-90 day
Laduca, J. and Sinha, D.
In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso-N-Methylurea Using a Collagen Gel Matrix Culture ,
In Vitro Cell Dev Biol
 29A , 789 , 1993
Cells
Epithelial
Enzymes
Medium
EBSS
Mammary
Rat
Rat, Lewis, female, 90 days
Lin, T. , Hom, Y.K. , Richards, J. and Nandi, S.
Effects of Antioxidants and Reduced Oxygen Tension on Rat Mammary Epithelial Cells in Culture ,
In Vitro Cell Dev Biol
 27A , 191 , 1991
Cells
Epithelial
Mammary
Enzymes
Collagenase : 0.05%
Medium
Medium 199
Mammary
Rat
Rat, SD, female, 55 day
Ehmann, U. , Osborn, R. , Guzman, R. and Fajardo, L.
Cultured Proliferating Rat Mammary Epithelial Cells ,
In Vitro Cell Dev Biol
 27 , 749 , 1991
Cells
Epithelial
Enzymes
Neutral Protease : 3 u/ml
Medium
Medium 199
Mammary
Rat
Rat, 50-60 days
Hahm, H.A. and Ip, M.M.
Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors ,
In Vitro Cell Dev Biol
 26 , 791 , 1990
Cells
Epithelial
Enzymes
Medium
EBSS
Mammary
Rat
Rat, Fisher 344, virgin, 80-100 day
McGrath, M. , Palmer, S. and Nandi, S.
Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF ,
J Cell Physiol
 125 , 182 , 1985
Cells
Epithelial
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Rat
Rat, LEW, virgin, female, 45-50 day
Ethler, S.
Primary Culture and Serial Passage of Normal and Carcinogen-Treated Rat Mammary Epithelial Cells In Vitro ,
J Natl Cancer Inst
 74 (6) , 1307 , 1985
Cells
Epithelial
Enzymes
Collagenase : 0.1%
Medium
Medium 199
Mammary
Rat
Rat, SD, female
Cohen, L.
Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
 18 , 565 , 1982
Cells
Epithelial, cancer and tumor
Enzymes
Collagenase : 0.1%
Medium
Eagles's MEM
Mammary
Rat
Rat, inbred LEW, female, 50-60 day old
Richards, J. and Nandi, S.
Primary Culture of Rat Mammary Epithelial Cells. I. Effect of Plating Density, Horomones, and Serum on DNA Synthesis ,
J Natl Cancer Inst
 61 (3) , 765 , 1978
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Rat
Rat, Wistar, 13-18 day postpartum
Katz, J. , Wals, P. and Van de Velde, R
Lipogenesis by Acini from Mammary Gland of Lactating Rats ,
J Biol Chem
 249 , 7348 , 1974
Cells
Acini
Enzymes
Collagenase : 0.05%
Medium
HBSS
Mammary
Rat
Rat, SD, 15-20 day postpartum
Martin, R. and Baldwin, R.
Effects of Insulin on Isolated Rat Mammary Cell Metabolism: Glucose Utilization and Metabolite Patterns ,
Endocrinology
 89 , 1263 , 1971
Cells
Mammary
Enzymes
Collagenase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Mammary
Rat
Rat, virgin
Moon, R. , Janns, D. and Young, S.
Preparation of Fat Cell-"Free" Rat Mammary Gland ,
J Histochem Cytochem
 17 (3) , 182 , 1969
Cells
Mammary
Enzymes
Collagenase : 0.35%
Medium
Medium 199
Pancreas
Bovine
Bovine
Cotton, C. and Al-Nakkash, L.
Isolation and Culture of Bovine Pancreatic Duct Epithelial cells ,
Am J Physiol
 272 , G1328 , 1997
Cells
Duct epithelial
Enzymes
Collagenase : 0.1%
Medium
HEPES
Pancreas
Bovine
Bovine (Bos taurus)
Sato, T. , Sato, M. , Hudson, E. and Jones, R.
Characterization of Bovine Pancreatic Ductal Cells Isolated by a Perfusion-Digestion Technique ,
In Vitro
 19 , 651 , 1983
Cells
Ductal
Enzymes
Medium
EBSS
Pancreas
Bovine
Bovine
Stiles, G. and Lefkowitz, R.
Hormone-Sensitive Adneylate Cyclase ,
J Biol Chem
 257 (11) , 6287 , 1982
Cells
Platelets
Medium
(see reference)
Pancreas
Canine
Canine
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Pancreas
Canine
Canine, adult mongrel, 15-25 kg
Noel, J. , Rabinovitch, A. , Olson, L. , Kyriakides, G. , Miller, J. and Mintz, D.
A Method for Large-Scale, High-Yield Isolation of Canine Pancreatic Islets of Langerhans ,
Metabolism
 31 (2) , 184 , 1982
Cells
Islets
Enzymes
Collagenase Type 4 : 600-1100 u/ml
Deoxyribonuclease I : 10 ug/ml
Medium
RPMI 1640
Pancreas
Fish
Fish, Osphronemus gourami, 3-month-old, 0.5 mg
Schrezenmeir, J. , Laue, C. , Sternheim,E. , Wolbert, K. , Darquy, S. , Chicheportiche, D. , Kirchgessner, J. and Reach, G.
Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers ,
Transplant Proc
 24 (6) , 2941 , 1992
Cells
Islets
Enzymes
Collagenase : 0.12 - 0.46 u/ml
Medium
RPMI 1640
Pancreas
Guinea-Pig
Guinea-pig
Schultz, G. , Sarras, Jr, M. , Gunther, G. , Hull, B. , Alicea, H. , Gorelick, F. and Jamieson, J.
Guinea Pig Pancreatic Acini Prepared with Purified collagenase ,
Exp Cell Res
 130 , 49 , 1980
Cells
Acinar
Enzymes
Collagenase Type 3 : 60 u/ml
Medium
Kreb's Ringer
Pancreas
Guinea-Pig
Guinea-pig, Hartley, albino, male, 350-400 g
Gardner, J. , Conlon, T. , Klaeveman, H. , Adams, T. and Ondetti, M.
Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells ,
J Clin Invest
 56 , 366 , 1975
Cells
Acinar
Enzymes
Medium
Kreb's Ringer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.