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Tissue References Search Tool

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Parotid
Mouse
Mouse
Ogawa, M. , Oshima, M. , Imamura, A. , Sekine, Y. , Ishida, K. , Yamashita, K. , Nakajima, K. , Hirayama, M. , Tachikawa, T. and Tsuji, T.
Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ. ,
Nat Commun
 4 , 2498 , 2013
Cells
Parotid and sublingual glandular
Enzymes
Neutral Protease : 50 u/ml
Collagenase Type 1 : 100 u/ml
Medium
PBS
Parotid
Rat
Rat, Wistar, male, 130 g
Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B.
Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. ,
Adv Dent Res
 14 , 62 , 2000
Cells
Acinar
Enzymes
Collagenase : 75 u/ml
Hyaluronidase : 153 u/ml
Medium
RPMI 1640
Parotid
Rat
Rat, SD, 100-120 g
D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL.
Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes ,
J Histochem Cytochem
 45 , 965-73 , 1997
Cells
Parotid acinar cells
Enzymes
Collagenase Type 2 : 90 u/ml
Medium
Krebs-Henseleit Bicarbonate
Parotid
Rat
Rat, SD, male
Prasad, K.N. , Edwards-Prasad, J. , Carvaiho, I. , LaRosa, F.G. , Baibinder, E. , Meyers, A. and Quissell, D.
Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments ,
In Vitro Cell Dev Biol
 28 , 493 , 1992
Cells
Epithelial
Enzymes
Collagenase : 50-75 u/ml
Hyaluronidase : 0.1%
Medium
HBSS CF
Parotid
Rat
Rat, SD, male, 100-150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Parotid
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Parotid
Rat
Rat
Takuma, T. and Ichida, T.
Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP ,
J Invest Dermatol
 103 , 95 , 1988
Cells
Parotid
Enzymes
Hyaluronidase : 0.025%
Medium
HBSS with 20mM HEPES
Parotid
Rat
Rat, Wistar, male, 250 - 350 g
Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R.
A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells ,
Biochem Biophys Res Commun
 145 , 754 , 1987
Cells
Acinar, parotid
Enzymes
Hyaluronidase : 0.015%
Medium
Earle's MEM
Parotid
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Parotid
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Pituitary
Bovine
Calf, male, less then 6 months old
Hassan, H. and Merkel, R.
Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells ,
In Vitro Cell Dev Biol
 30A , 435 , 1994
Cells
Pituitary
Enzymes
Collagenase : 0.3%
Medium
DMEM
Pituitary
Bovine
Bovine
Mason, W. and Ingram, C.
Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells , Vol. 124 , 207 , 1986
Cells
Pituitary
Enzymes
Collagenase : 0.1%
Medium
EBSS, CMF
Pituitary
Bovine
Bovine
Ferrara, N. , Goldsmith, P. , Fujii, D. and Weiner, R.
Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis , Vol. 124 , 245 , 1986
Cells
Follicular, anterior pituitary and pars tuberalis
Enzymes
Deoxyribonuclease I : 200 µg/ml
Medium
HBSS, CMF
Pituitary
Bovine
Calf, male, 1-6 week old
Ridgway, E. , Klibanski, A. , Marorana, M. , Milbury, P. , Kieffer, J. and Chin, W.
The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro ,
Endocrinology
 112 (6) , 1937 , 1983
Cells
Pitituary
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM
Pituitary
Mouse
Mouse
Perez Millain, M. , Brinkmeier, M. , Mortensen, A. and Camper, S.
PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells. ,
Elife
 5 , 2016
Cells
Pituitary
Enzymes
Medium
HBSS
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Pituitary
Mouse
Mouse, male
Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A.
Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase ,
Endocrinology
 144 , 188-200 , 2003
Cells
Pituitary
Enzymes
Collagenase : 0.4%
Hyaluronidase : 0.1%
Trypsin : 0.3%
Medium
DMEM/Han's F12
Pituitary
Ovine
Ovine, adult
Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen.
Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes ,
Endocrinology
 143 , 4609-19 , 2002
Cells
Somatotropes
Enzymes
Medium
Medium 199
Pituitary
Rat
Rat, male, 12-15 week
Akieda-Asai, S. , Zaima, N. , Ikegami, K. , Kahyo, T, Yao, I. , Hatanaka, T. , Iemura, S. , Sugiyama, R. , Yokozeki, T. , Eishi, Y. , Koike, M. , Ikeda, K. , Chiba, T. , Yamaza, H. , Shimokawa, I. , Song, S. , Matsuno, A. , Mizutani, A. , Sawabe, M. Chao, N. and Tanaka, M.
SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals. ,
PLoS ONE
 5 , e11755 , 2010
Cells
Pituitary
Enzymes
Medium
DMEM
Pituitary
Rat
Rat, Wistar, male, 2 month old
Zhou, X. , De Schepper, J. , De Craemer, D. , Delhase, M. , Gys, G. , Smitz, J. and Hooghe-Peters, E.
Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats ,
J Endocrinol
 159 , 165 , 1998
Cells
Pituitary
Enzymes
Trypsin : 0.1%
Medium
DMEM
Pituitary
Rat
Rat, SD, female, 200 - 250 g
D'Emden, M. and Wark, J.
Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion ,
In Vitro Cell Dev Biol
 27 , 197 , 1991
Cells
Anterior pituitary gland
Enzymes
Trypsin : 0.1%
Medium
EBSS, CMF
Pituitary
Rat
Rat
Wilfinger, W. , Larsen, W. , Downs, T. and Wilbur, D.
An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells ,
Tissue Cell
 16 (4) , 483 , 1984
Cells
Pituitary
Enzymes
Trypsin : 0.3 %
Medium
HEPES
Pituitary
Rat
Rat, SD, male, 250-450 g
Portanova, R. , Smith, D. and Sayers, G.
A Trypsin for the Preparation of Isolated Rat Anterior Pituitary ,
Proc Soc Exp Biol Med
 133 , 573 , 1970
Cells
Anterior pituitary
Enzymes
Trypsin : 0.25%
Medium
Krebs
Thyroid/Parathyroid
Bovine
Bovine, adult
Nygren, P. , Gylfe, E. , Larsson, R. , Johansson, H. , Juhlin, C. , Klareskoq, L. , Akerstrom, G. and Rastad, J.
Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism ,
Biochim Biophys Acta
 968 , 253 , 1988
Cells
Parathyroid
Enzymes
Medium
HEPES Ham's F10
Thyroid/Parathyroid
Bovine
Bovine
Wallace, J. and Scarpa, A.
Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations ,
J Biol Chem
 257 , 10613 , 1982
Cells
Parathyroid glands
Enzymes
Medium
HEPES buffer
Thyroid/Parathyroid
Bovine
Bovine
Brown, E. , Hurwitz, S. and Aurbach, G.
Preparation of Viable Isolated Bovine Parathyroid Cells ,
Endocrinology
 99 , 1582 , 1976
Cells
Parathyroid
Enzymes
Medium
Eagle's #2 medium without bicarbonate
Thyroid/Parathyroid
Bovine
Bovine
(also porcine)
Tong, W.
The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
 32 , 745 , 1974
Cells
Thyroid
Enzymes
Trypsin : 0.004%
Medium
EBSS
Thyroid/Parathyroid
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Thyroid/Parathyroid
Chicken
Chicken, Rhode Island Red, embryo
Spooner, B.
The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture ,
J Cell Physiol
 75 , 33 , 1970
Cells
Thyroid follicular
Enzymes
Collagenase : 0.2%
Medium
Tyrode's solution, CMF
Thyroid/Parathyroid
Human

Human

Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y.
Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022
Cells
Thyroid normal and tumor
Enzymes
Trypsin : 0.125%
Collagenase Type 4 : 300 u/ml
Medium
RPMI-1640
Thyroid/Parathyroid
Human
Human
Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.
Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
 284 , E168 , 2003
Cells
Endothelial
Enzymes
Medium
DMEM
Thyroid/Parathyroid
Human
Human
Gianoukakis, A. , Cao, H. , Jennings, T. and Smith, T.
Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells. ,
Am J Physiol Cell Physiol
 280 , C701 , 2001
Cells
Thyrocytes
Enzymes
Collagenase Type 1 : 130 u/ml
Neutral Protease : 0.5 u/ml
Medium
HBSS
Thyroid/Parathyroid
Human
Human
Howie, A. , Walker, S. , Akesson, B. , Arthur, J. and Beckett, G.
Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis. ,
Biochem J
 308 ( Pt 3) , 713 , 1995
Cells
Thyrocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.1%
Medium
EBSS
Thyroid/Parathyroid
Human
Human
Miller, R. , Hiraoka, T. , Nakamura, N. , Tenou, H. , Kopecky, K. , Jones, M. and Gould, M.
In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology. ,
J Radiat Res
 26 , 269 , 1985
Cells
Thyroid
Enzymes
Collagenase : 300 u/ml
Medium
Ham's F-12/MEM
Thyroid/Parathyroid
Mouse
Mouse
Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.
Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
 33 , 1657-69 , 2015
Cells
Thyroid tumor
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM
Thyroid/Parathyroid
Mouse
Mouse, 6 week
Martin, A. , Coronel, E. , Sano, G. , Chen, S. , Vassileva, G. , Canasto-Chibuque, C. , Sedgwick, J. , Frenette, P. , Lipp, M. , Furtado, G. and Lira, S.
A Novel Model for Lymphocytic Infiltration of the Thyroid Gland Generated by Transgenic Expression of the CC Chemokine CCL21. ,
J Immunol
 173 , 4791 , 2004
Cells
Thyroid
Enzymes
Neutral Protease : 0.0012 u/ml
Collagenase Type 2 : 0.25 u/ml
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.