Tissue References Search Tool

Rat

Rat, Wistar, male, 130 g

Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B.

Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. ,
Adv Dent Res
14 , 62 , 2000

Cells

Acinar

Enzymes

Collagenase : 75 u/ml

Hyaluronidase : 153 u/ml

Medium

RPMI 1640

Rat

Rat, SD, 100-120 g

D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL.

Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes ,
J Histochem Cytochem
45 , 965-73 , 1997

Cells

Parotid acinar cells

Enzymes

Collagenase Type 2 : 90 u/ml

Medium

Krebs-Henseleit Bicarbonate

Rat

Rat, SD, male

Prasad, K.N. , Edwards-Prasad, J. , Carvaiho, I. , LaRosa, F.G. , Baibinder, E. , Meyers, A. and Quissell, D.

Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments ,
In Vitro Cell Dev Biol
28 , 493 , 1992

Cells

Epithelial

Enzymes

Collagenase : 50-75 u/ml

Hyaluronidase : 0.1%

Medium

HBSS CF

Rat

Rat, SD, male, 100-150 g

Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.

Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
27 , 707 , 1991

Cells

Parotid acinar

Enzymes

Trypsin : 0.001%

Medium

F12 medium

Rat

Rat, Wistar, male

Foskett, J. , Roifman, C. and Wong, D.

Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
266 , 2778 , 1991

Cells

Parotid acinar

Enzymes

Trypsin : 0.02%

Medium

Solution B

(See reference)

Rat

Takuma, T. and Ichida, T.

Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP ,
J Invest Dermatol
103 , 95 , 1988

Cells

Parotid

Enzymes

Hyaluronidase : 0.025%

Medium

HBSS with 20mM HEPES

Rat

Rat, Wistar, male, 250 - 350 g

Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R.

A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells ,
Biochem Biophys Res Commun
145 , 754 , 1987

Cells

Acinar, parotid

Enzymes

Hyaluronidase : 0.015%

Medium

Earle's MEM

Rat

Rat, SD, male, 50 - 125 g

Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.

Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
23 , 465 , 1987

Cells

Acinar

Exorbital lacrimal, parotid, pancreas

Enzymes

Trypsin : 0.01%

Medium

HBSS, CMF

Rat

Rat, SD, albino, male, 150-200 g

Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.

Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
229 (3) , 560 , 1975

Cells

Acinar, parotid

Enzymes

Trypsin : 0.01%

Collagenase : 40-50 u/ml

Hyaluronidase : 0.10%

Medium

HBSS CMF

Bovine

Bovine, adult

Nygren, P. , Gylfe, E. , Larsson, R. , Johansson, H. , Juhlin, C. , Klareskoq, L. , Akerstrom, G. and Rastad, J.

Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism ,
Biochim Biophys Acta
968 , 253 , 1988

Cells

Parathyroid

Enzymes

Deoxyribonuclease I : 0.005%,

Medium

HEPES Ham's F10

Bovine

Wallace, J. and Scarpa, A.

Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations ,
J Biol Chem
257 , 10613 , 1982

Cells

Parathyroid glands

Enzymes

Deoxyribonuclease I : 0.0075%

Medium

HEPES buffer

Bovine

Brown, E. , Hurwitz, S. and Aurbach, G.

Preparation of Viable Isolated Bovine Parathyroid Cells ,
Endocrinology
99 , 1582 , 1976

Cells

Parathyroid

Enzymes

Deoxyribonuclease I : 0.004%

Medium

Eagle's #2 medium without bicarbonate

Bovine

Bovine
(also porcine)

Tong, W.

The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
32 , 745 , 1974

Cells

Thyroid

Enzymes

Trypsin : 0.004%

Medium

EBSS

Chicken

Chick embryo

Hilfer, S. and Brown, J.

Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
65 , 246 , 1971

Cells

Thyroid

Muscle

Heart

Enzymes

Collagenase : 0.25%

Medium

Tyrode's saline, potassium free

Chicken

Chicken, Rhode Island Red, embryo

Spooner, B.

The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture ,
J Cell Physiol
75 , 33 , 1970

Cells

Thyroid follicular

Enzymes

Collagenase : 0.2%

Medium

Tyrode's solution, CMF

Human

Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y.

Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022

Cells

Thyroid normal and tumor

Enzymes

Trypsin : 0.125%

Collagenase Type 4 : 300 u/ml

Medium

RPMI-1640

Human

Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.

Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
284 , E168 , 2003

Cells

Endothelial

Enzymes

Collagenase Type 2 : 0.1%

Medium

DMEM

Human

Gianoukakis, A. , Cao, H. , Jennings, T. and Smith, T.

Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells. ,
Am J Physiol Cell Physiol
280 , C701 , 2001

Cells

Thyrocytes

Enzymes

Collagenase Type 1 : 130 u/ml

Neutral Protease : 0.5 u/ml

Medium

HBSS

Human

Howie, A. , Walker, S. , Akesson, B. , Arthur, J. and Beckett, G.

Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis. ,
Biochem J
308 ( Pt 3) , 713 , 1995

Cells

Thyrocytes

Enzymes

Neutral Protease : 0.5%

Trypsin : 0.25%

Collagenase : 0.1%

Medium

EBSS

Human

Miller, R. , Hiraoka, T. , Nakamura, N. , Tenou, H. , Kopecky, K. , Jones, M. and Gould, M.

In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology. ,
J Radiat Res
26 , 269 , 1985

Cells

Thyroid

Enzymes

Collagenase : 300 u/ml

Medium

Ham's F-12/MEM

Mouse

Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.

Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
33 , 1657-69 , 2015

Cells

Thyroid tumor

Enzymes

Collagenase Type 1 : 300 u/ml

Hyaluronidase : 100 u/ml

Deoxyribonuclease I : 0.01%

Medium

DMEM

Mouse

Mouse, 6 week

Martin, A. , Coronel, E. , Sano, G. , Chen, S. , Vassileva, G. , Canasto-Chibuque, C. , Sedgwick, J. , Frenette, P. , Lipp, M. , Furtado, G. and Lira, S.

A Novel Model for Lymphocytic Infiltration of the Thyroid Gland Generated by Transgenic Expression of the CC Chemokine CCL21. ,
J Immunol
173 , 4791 , 2004

Cells

Thyroid

Enzymes

Neutral Protease : 0.0012 u/ml

Collagenase Type 2 : 0.25 u/ml

Medium

RPMI 1640

Ovine

Sheep

Kerkof, P.

Preparation of Primary Cultures of Ovine Thyroid Gland Cells ,
J Tiss Cul Meth
7 , 23 , 1982

Cells

Thyroid

Enzymes

Collagenase : 0.2%

Medium

Puck's Saline F

Porcine

Porcine
(also bovine)

Tong, W.

The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
32 , 745 , 1974

Cells

Thyroid

Enzymes

Trypsin : 0.004%

Medium

EBSS

Rat

Rat, Lewis, male, 4 week

Arauchi, A. , Shimizu, T. , Yamato, M. , Obara, T. and Okano, T.

Tissue-Engineered Thyroid Cell Sheet Rescued Hypothyroidism in Rat Models After Receiving Total Thyroidectomy Comparing with Nontransplantation Models. ,
Tissue Eng Part A
15 , 3943 , 2009

Cells

Thyroid

Enzymes

Collagenase Type 2 : 0.15%

Collagenase Type 4 : 0.15%

Medium

DMEM

Canine

Canine, adult

Petrouškova, P. , Hudáková, N. , Almášiová, V. , Valenčáková, A. , Horňáková, L. , Huniadi, M. and Čížková, D.

Establishment of Primary Cell Cultures from Canine Mammary Gland Malignant Tumours: A Preliminary Study. , J Vet Res Vol. 69 , 159-168 , 2025

Cells

Mammary gland tumor

Enzymes

Collagenase Type 4 : 0.05%

Medium

EBSS

Hamster

Hamster, 90-100 g

Gonzalez, A. , Oberley, T. , Schultz, J. , Ostrom, J. and Li, J.

In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney ,
In Vitro Cell Dev Biol
29A , 562 , 1993

Cells

Tumor

Enzymes

Hyaluronidase : 0.1%

Medium

Waymouth's MB

Hamster

Hamster, 6 week old

Min, B. , Kim, K. , Cherrick, H. and Park, N.

Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation ,
In Vitro Cell Dev Biol
27A , 128 , 1991

Cells

Buccal pouch

Enzymes

Neutral Protease : 0.24%

Medium

CMF HBSS

Human

Song, H. , Weinstein, H. , Allegakoen, P. , Wadsworth, M. , Xie, J. , Yang, H. , Castro, E. , Lu, K. , Stohr, B. , Feng, F. , Carroll, P. , Wang, B. , Cooperberg, M. , Shalek, A. and Huang, F.

Single-cell Analysis of Human Primary Prostate Cancer Reveals the Heterogeneity of Tumor-associated Epithelial Cell States. ,
Nat Commun
13 , 141 , 2022

Cells

Prostate cells

Enzymes

Collagenase Type 4 : 1,000 u/ml

Medium

RPMI

Human

Jeske, S. , Schuler, P. , Doescher, J. , Theodoraki, M. , Laban, S. , Brunner, C. , Hoffman, T. and Wigand, M.

Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma ,
Immun Ageing
17 , 3 , 2020

Cells

Tumor infiltrating lympocytes

Enzymes

Collagenase Type 1 : 200 u/ml

Medium

RPMI

Human

Ayuso, J. , Vitek, R. , Swick, A. , Skala, M. , Wisinski, K. , Kimple, R. , Lambert, P. and Beebe, D.

Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells ,
Sci Rep
9 , 12480 , 2019

Cells

Fibroblasts

Enzymes

Collagenase Type 1 : 0.5%

Neutral Protease : 0.1%

Deoxyribonuclease I : 1,000 u/ml

Medium

FM Fibroblast media

Human

Phan, N. , Hong, J. , Tofig, B. , Mapua, M. , Elashoff, D. , Moatamed, N. , Huang, J. , Memarzadeh, S. , Damoiseaux, R. and Soragni, A.

A Simple High-Throughput Approach Identifies Actionable Drug Sensitivities in Patient-Derived Tumor Organoids. ,
Commun Biol
2 , 78 , 2019

Cells

Tumor

Enzymes

Collagenase Type 4 : 200 u/ml

Medium

DMEM

Human

Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al

Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
175 , 1972 , 2018

Cells

Organoids

Enzymes

Collagenase Type 4 : 200-300 u/ml

Medium

DMEM

Human

Jiang, C. , Zhang, Q. , Shanti, R. , Shi, S. , Chang, T. , Carrasco, L. , Alawi, F. and Le, A.

Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells. ,
Stem Cells
35 , 2083-2094 , 2017

Cells

Ameloblastoma epithelial

Enzymes

Collagenase : 0.1%

Neutral Protease : 50 u/ml

Medium

MEM