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Tissue References Search Tool

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Parotid
Mouse
Mouse
Ogawa, M. , Oshima, M. , Imamura, A. , Sekine, Y. , Ishida, K. , Yamashita, K. , Nakajima, K. , Hirayama, M. , Tachikawa, T. and Tsuji, T.
Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ. ,
Nat Commun
 4 , 2498 , 2013
Cells
Parotid and sublingual glandular
Enzymes
Neutral Protease : 50 u/ml
Collagenase Type 1 : 100 u/ml
Medium
PBS
Parotid
Rat
Rat, Wistar, male, 130 g
Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B.
Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. ,
Adv Dent Res
 14 , 62 , 2000
Cells
Acinar
Enzymes
Collagenase : 75 u/ml
Hyaluronidase : 153 u/ml
Medium
RPMI 1640
Parotid
Rat
Rat, SD, 100-120 g
D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL.
Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes ,
J Histochem Cytochem
 45 , 965-73 , 1997
Cells
Parotid acinar cells
Enzymes
Collagenase Type 2 : 90 u/ml
Medium
Krebs-Henseleit Bicarbonate
Parotid
Rat
Rat, SD, male
Prasad, K.N. , Edwards-Prasad, J. , Carvaiho, I. , LaRosa, F.G. , Baibinder, E. , Meyers, A. and Quissell, D.
Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments ,
In Vitro Cell Dev Biol
 28 , 493 , 1992
Cells
Epithelial
Enzymes
Collagenase : 50-75 u/ml
Hyaluronidase : 0.1%
Medium
HBSS CF
Parotid
Rat
Rat, SD, male, 100-150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Parotid
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Parotid
Rat
Rat
Takuma, T. and Ichida, T.
Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP ,
J Invest Dermatol
 103 , 95 , 1988
Cells
Parotid
Enzymes
Hyaluronidase : 0.025%
Medium
HBSS with 20mM HEPES
Parotid
Rat
Rat, Wistar, male, 250 - 350 g
Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R.
A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells ,
Biochem Biophys Res Commun
 145 , 754 , 1987
Cells
Acinar, parotid
Enzymes
Hyaluronidase : 0.015%
Medium
Earle's MEM
Parotid
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Parotid
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Villarreal, E. , Hewgley, W. , Lang, W. , Morton, C. , Mao, S. , Wu, J. and Sandoval, J.
In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation? ,
J Surg Res
 218 , 209-216 , 2017
Cells
Speen
Enzymes
Medium
HBSS
Spleen
Mouse
Mouse
Klebanoff, C. , Spencer, S. , Torabi-Parizi, P. , Grainger, J. , Roychoudhuri, R. , Ji, Y. , Sukumar, M. , Muranski, P. , Scott, C. , Hall, J. , Ferreyra, G. , Leonardi, A. and Borman, Z. et al.
Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells. ,
J Exp Med
 210 , 1961 , 2013
Cells
Splenic
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Yi, T. and Cyster, J.
EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture. ,
Elife
 2 , e00757 , 2013
Cells
Splenocytes
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Flano, E. , Jewell, N. , Durbin, R. and Durbin, J.
Methods Used to Study Respiratory Virus Infection. , Curr Protoc Cell Biol Vol. Chapter 26 , Unit 26.3 , 2009
Cells
Dendritic
Enzymes
Medium
HBSS
Spleen
Mouse
Mouse, female, 6-8 week
Abou Fakher, F. , Rachinel, N. , Klimczak, M. , Louis, J. and Doyen, N.
TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions. ,
J Immunol
 182 , 1386 , 2009
Cells
Dendritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Kang, H. , Liu, M. and Datta, S.
Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells ,
J Immunol
 178 , 7849-58 , 2007
Cells
Splenic dendritic
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Mueller, S. , Matloubian, M. , Clemens, D, Sharpe, A. , Freeman, G. , Gangappa, S. , Larsen, C. and Ahmed, R.
Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection. ,
Proc Natl Acad Sci U S A
 104 , 15430 , 2007
Cells
Splenic
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse
Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F.
Specific remodeling of splenic architecture by cytomegalovirus ,
PLoS Pathog
 2 , e16 , 2006
Cells
Splenic stromal
Enzymes
Collagenase Type 3 : 100-400 u/ml
Medium
HBSS
Spleen
Mouse
Mouse
Siragam, V. , Crow, A. , Brinc, D. , Song, S. , Freedman, J. and Lazarus, A.
Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells. ,
Nat Med
 12 , 688 , 2006
Cells
Leukocytes
Enzymes
Collagenase Type 4 : 43 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse, 4-6 week
McLellan, A. , Kapp, M. , Eggert, A. , Linden, C. , Bommhardt, U. , Brocker, E. , Kammerer, U. and Kampgen, E.
Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression ,
Blood
 99 , 2084-93 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI-1640
Spleen
Mouse
Mouse
Stagg, AJ, Burke, F, Hill, S. and Knight, S.
Isolation of Mouse Spleen Dendritic Cells. ,
Methods Mol Med
 64 , 9-22 , 2001
Cells
Dentritic cells
Enzymes
Medium
HEPES/RPMI 1640
Spleen
Mouse
Mouse
Brasel K, De Smedt T, Smith JL, Maliszewski CR.
Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures ,
Blood
 96 , 3029-39 , 2000
Cells
Dentritic
Enzymes
Collagenase : 100 u/ml
Medium
HBSS
Thyroid/Parathyroid
Bovine
Bovine, adult
Nygren, P. , Gylfe, E. , Larsson, R. , Johansson, H. , Juhlin, C. , Klareskoq, L. , Akerstrom, G. and Rastad, J.
Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism ,
Biochim Biophys Acta
 968 , 253 , 1988
Cells
Parathyroid
Enzymes
Medium
HEPES Ham's F10
Thyroid/Parathyroid
Bovine
Bovine
Wallace, J. and Scarpa, A.
Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations ,
J Biol Chem
 257 , 10613 , 1982
Cells
Parathyroid glands
Enzymes
Medium
HEPES buffer
Thyroid/Parathyroid
Bovine
Bovine
Brown, E. , Hurwitz, S. and Aurbach, G.
Preparation of Viable Isolated Bovine Parathyroid Cells ,
Endocrinology
 99 , 1582 , 1976
Cells
Parathyroid
Enzymes
Medium
Eagle's #2 medium without bicarbonate
Thyroid/Parathyroid
Bovine
Bovine
(also porcine)
Tong, W.
The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
 32 , 745 , 1974
Cells
Thyroid
Enzymes
Trypsin : 0.004%
Medium
EBSS
Thyroid/Parathyroid
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Thyroid/Parathyroid
Chicken
Chicken, Rhode Island Red, embryo
Spooner, B.
The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture ,
J Cell Physiol
 75 , 33 , 1970
Cells
Thyroid follicular
Enzymes
Collagenase : 0.2%
Medium
Tyrode's solution, CMF
Thyroid/Parathyroid
Human

Human

Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y.
Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022
Cells
Thyroid normal and tumor
Enzymes
Trypsin : 0.125%
Collagenase Type 4 : 300 u/ml
Medium
RPMI-1640
Thyroid/Parathyroid
Human
Human
Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.
Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
 284 , E168 , 2003
Cells
Endothelial
Enzymes
Medium
DMEM
Thyroid/Parathyroid
Human
Human
Gianoukakis, A. , Cao, H. , Jennings, T. and Smith, T.
Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells. ,
Am J Physiol Cell Physiol
 280 , C701 , 2001
Cells
Thyrocytes
Enzymes
Collagenase Type 1 : 130 u/ml
Neutral Protease : 0.5 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.