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Tissue References Search Tool

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Parotid
Mouse
Mouse
Ogawa, M. , Oshima, M. , Imamura, A. , Sekine, Y. , Ishida, K. , Yamashita, K. , Nakajima, K. , Hirayama, M. , Tachikawa, T. and Tsuji, T.
Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ. ,
Nat Commun
 4 , 2498 , 2013
Cells
Parotid and sublingual glandular
Enzymes
Neutral Protease : 50 u/ml
Collagenase Type 1 : 100 u/ml
Medium
PBS
Parotid
Rat
Rat, Wistar, male, 130 g
Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B.
Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. ,
Adv Dent Res
 14 , 62 , 2000
Cells
Acinar
Enzymes
Collagenase : 75 u/ml
Hyaluronidase : 153 u/ml
Medium
RPMI 1640
Parotid
Rat
Rat, SD, 100-120 g
D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL.
Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes ,
J Histochem Cytochem
 45 , 965-73 , 1997
Cells
Parotid acinar cells
Enzymes
Collagenase Type 2 : 90 u/ml
Medium
Krebs-Henseleit Bicarbonate
Parotid
Rat
Rat, SD, male
Prasad, K.N. , Edwards-Prasad, J. , Carvaiho, I. , LaRosa, F.G. , Baibinder, E. , Meyers, A. and Quissell, D.
Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments ,
In Vitro Cell Dev Biol
 28 , 493 , 1992
Cells
Epithelial
Enzymes
Collagenase : 50-75 u/ml
Hyaluronidase : 0.1%
Medium
HBSS CF
Parotid
Rat
Rat, SD, male, 100-150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Parotid
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Parotid
Rat
Rat
Takuma, T. and Ichida, T.
Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP ,
J Invest Dermatol
 103 , 95 , 1988
Cells
Parotid
Enzymes
Hyaluronidase : 0.025%
Medium
HBSS with 20mM HEPES
Parotid
Rat
Rat, Wistar, male, 250 - 350 g
Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R.
A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells ,
Biochem Biophys Res Commun
 145 , 754 , 1987
Cells
Acinar, parotid
Enzymes
Hyaluronidase : 0.015%
Medium
Earle's MEM
Parotid
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Parotid
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Villarreal, E. , Hewgley, W. , Lang, W. , Morton, C. , Mao, S. , Wu, J. and Sandoval, J.
In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation? ,
J Surg Res
 218 , 209-216 , 2017
Cells
Speen
Enzymes
Medium
HBSS
Spleen
Mouse
Mouse
Klebanoff, C. , Spencer, S. , Torabi-Parizi, P. , Grainger, J. , Roychoudhuri, R. , Ji, Y. , Sukumar, M. , Muranski, P. , Scott, C. , Hall, J. , Ferreyra, G. , Leonardi, A. and Borman, Z. et al.
Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells. ,
J Exp Med
 210 , 1961 , 2013
Cells
Splenic
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Yi, T. and Cyster, J.
EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture. ,
Elife
 2 , e00757 , 2013
Cells
Splenocytes
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Flano, E. , Jewell, N. , Durbin, R. and Durbin, J.
Methods Used to Study Respiratory Virus Infection. , Curr Protoc Cell Biol Vol. Chapter 26 , Unit 26.3 , 2009
Cells
Dendritic
Enzymes
Medium
HBSS
Spleen
Mouse
Mouse, female, 6-8 week
Abou Fakher, F. , Rachinel, N. , Klimczak, M. , Louis, J. and Doyen, N.
TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions. ,
J Immunol
 182 , 1386 , 2009
Cells
Dendritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Kang, H. , Liu, M. and Datta, S.
Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells ,
J Immunol
 178 , 7849-58 , 2007
Cells
Splenic dendritic
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Mueller, S. , Matloubian, M. , Clemens, D, Sharpe, A. , Freeman, G. , Gangappa, S. , Larsen, C. and Ahmed, R.
Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection. ,
Proc Natl Acad Sci U S A
 104 , 15430 , 2007
Cells
Splenic
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse
Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F.
Specific remodeling of splenic architecture by cytomegalovirus ,
PLoS Pathog
 2 , e16 , 2006
Cells
Splenic stromal
Enzymes
Collagenase Type 3 : 100-400 u/ml
Medium
HBSS
Spleen
Mouse
Mouse
Siragam, V. , Crow, A. , Brinc, D. , Song, S. , Freedman, J. and Lazarus, A.
Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells. ,
Nat Med
 12 , 688 , 2006
Cells
Leukocytes
Enzymes
Collagenase Type 4 : 43 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse, 4-6 week
McLellan, A. , Kapp, M. , Eggert, A. , Linden, C. , Bommhardt, U. , Brocker, E. , Kammerer, U. and Kampgen, E.
Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression ,
Blood
 99 , 2084-93 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI-1640
Spleen
Mouse
Mouse
Stagg, AJ, Burke, F, Hill, S. and Knight, S.
Isolation of Mouse Spleen Dendritic Cells. ,
Methods Mol Med
 64 , 9-22 , 2001
Cells
Dentritic cells
Enzymes
Medium
HEPES/RPMI 1640
Spleen
Mouse
Mouse
Brasel K, De Smedt T, Smith JL, Maliszewski CR.
Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures ,
Blood
 96 , 3029-39 , 2000
Cells
Dentritic
Enzymes
Collagenase : 100 u/ml
Medium
HBSS
Stem
Canine
23-33 kg
Williams, S. , Kosnik, P. , Kleinert, L. , Vossman, E. , Lye, K. and Shine, M.
Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts. ,
Tissue Eng Part A
 19 , 1295-302 , 2013
Cells
Stromal vascular
Enzymes
Collagenase : see reference
Medium
Media 199E
Stem
Canine
Canine
Park, S. , Seo, M. , Kim, H. and Kang, K.
Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells. ,
PLoS ONE
 7 , e44693 , 2012
Cells
Amniotic membrane MSC
Enzymes
Trypsin : 0.25%
Medium
LG-DMEM
Stem
Canine
Canine, 20-25 kg
Fischer, L. , McIlhenny, S. , Tulenko, T. , Golesorkhi, N. , Zhang, P. , Larson, R. , Lombardi, J. , Shapiro, I. and DiMuzio, P.
Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. ,
J Surg Res
 152 , 157 , 2009
Cells
Adipose stem cells
Enzymes
Collagenase : see reference
Medium
Media-199
Stem
Equine
Equine
Vidal, M. , Robinson, S. , Lopez, M. , Paulsen, D. , Borkhsenious, O. , Johnson, J. , Moore, R. and Gimble, J.
Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow. ,
Vet Surg
 37 , 713 , 2008
Cells
Adipose derived stem cells
Enzymes
Medium
PBS
Stem
Equine
Equine, 1-5 year
Vidal, M. , Kilroy, G. , Lopez, M. , Johnson, J. , Moore, R. and Gimble, J.
Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells. ,
Vet Surg
 36 , 613 , 2007
Cells
Adipose derived stem cells
Enzymes
Medium
PBS
Stem
Human
Human
Alstrup, T. , Eijken, M. , Bohn, A. , Moller, B. and Damsgaard, T.
Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. ,
Curr Protoc Stem Cell Biol
 48 , e68 , 2019
Cells
Adipose derived stem
Enzymes
Collagenase Type 4 : 150-300 u/ml
Medium
RPMI-1640
Stem
Human
Human
Yam, G. , Teo, E. , Setiawan, M. , Lovatt, M. , Yusoff, N. , Fuest, M. , Goh, B. and Mehta, J.
Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy. ,
J Cell Mol Med
 , 2018
Cells
Postnatal periodontal ligament
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Matsumura, E. , Tsuji, K. , Komori, K. , Koga, H. , Sekiya, I. and Muneta, T.
Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 19 , 181-193 , 2017
Cells
Synovial mesenchymal stem
Enzymes
Collagenase : 0.3%
Medium
MEM
Stem
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.